Thermal Analysis of Sulphamethoxazole - Sugar Physical Mixes

Differential scanning calorimetry and hot stage microscopy have been used to study the interactions between sulphamethoxazole and the sugars maltose, glucose, sucrose and mannitol. Only the sulphamethoxazole-mannitol system appeared to be stable and presented a eutectic containing 90.3% sulphamethoxazole. Heats of fusion for sulphamethoxazole, mannitol and their eutectic were 33.4, 73.6 and 39.9 cal g respectively.     

The labeled maximum matching problem

Given a graph G where a label is associated with each edge, we address the problem of looking for a maximum matching of G using the minimum number of different labels, namely the labeled maximum matching problem. It is a relatively new problem whose application is related to the timetabling problem. We prove it is NP-complete and present four different mathematical formulations. Moreover, we propose an exact algorithm based on a branch-and-bound approach to solve it. We evaluate the performance of our algorithm on a wide set of instances and compare our computational times with the ones required by CPLEX to solve the proposed mathematical formulations. Test results show the effectiveness of our procedure, that hugely outperforms the solver.     

New Linkage with Linear Actuator for Tracking PV Systems with Large Angular Stroke

This paper focuses on the development of an optimized photovoltaic tracking system involving low-cost, relative simple mechanisms, with linear actuators able to insure strokes comparable with those resulted when using gear rotary actuators. Starting with a rhombus linkage, with a linear actuator on the diagonal used for the elevation motion till 90°, a new performance solution is generated. This new linkage allows large angular strokes by using an asymmetric rhombus and an eccentrically positioned linear ac...     

Characterization of interactions between Escherichia coli O157:H7 with epiphytic bacteria in vitro and on spinach leaf surfaces

This study characterized the types of interactions between Escherichia coli O157:H7 and spinach phylloepiphytic bacteria and identified those that influence persistence of E. coli O157:H7 on edible plants. A total of 1512 phylloepiphytic bacterial isolates were screened for their ability to inhibit or to enhance the growth of E. coli O157:H7 in vitro and on spinach leaf surfaces. Fifteen different genera, the majority belonging to Firmicutes and Enterobacteriaceae, reduced growth rates of E. coli O157:H7 in vitro by either nutrient competition or acid production. Reduced numbers of E. coli O157:H7 were recovered from detached spinach leaves that were co-inoculated with epiphytic isolates belonging to five genera. A 1.8 log reduction in E. coli O157:H7 was achieved when co-inoculated with Erwinina perscinia and 20% cellobiose, a carbon source used by the phylloepiphytes but not E. coli O157:H7. The reduction on leaves was significantly less than reduction measured in vitro. Phylloepiphytic bacteria belonging to eight different genera, increased numbers of E. coli O157:H7 when co-cultured in vitro on spent medium and when co-cultured on detached spinach leaves. The results, showing reduction of E. coli O157:H7 numbers by natural epiphytic bacteria, support the hypothesis that native plant microbiota can be used for bio-control of foodborne pathogens, however, other epiphytes may promote the persistence of enteric pathogens on the phyllosphere.     

A Rapid Size-Exclusion Solid-Phase Extraction Step for Enhanced Sensitivity in Multi-Allergen Determination in Dark Chocolate and Biscuits by Liquid Chromatography–Tandem Mass Spectrometry

Enhanced sensitivity for the simultaneous determination of five nut allergens in biscuit and in dark chocolate complex matrices was obtained by introduction of a rapid size-exclusion solid-phase extraction-based step before liquid chromatography–electrospray ionization-tandem mass spectrometry (LC-ESI-MS²) analysis. A very fast and efficient separation (<12 min) of marker peptides with selected reaction monitoring detection was obtained. Limits of detection in the 0.1–1.3 mg nut/kg and 5–15 mg nut/kg ranges for biscuit and dark chocolate samples as well as high recoveries (84(±6)–106(±4)% for biscuits and 98(±5)–108(±6)% for dark chocolate) proved the excellent capabilities of the exploited sample treatment method combined with the LC-MS² analysis. Good precision in terms of intra- and inter-day repeatability was calculated, being always lower than 19 % (n?=?75). Linearity was demonstrated up to four and three orders of magnitude for biscuit and dark chocolate, respectively. Finally, the validated method was successfully applied to the investigation of hidden nut trace allergens in commercially available biscuits and chocolates of different brands aiming to ascertain possible discrepancies between allergen content and food allergen labelling.     

MedISys: An early-warning system for the detection of (re-)emerging food- and feed-borne hazards

We evaluated the Medical Information System (MedISys) as an early-warning system for the detection of food- and feed-borne hazards. Nine hazards were selected in the period from January 2007 to March 2009 from the Rapid Alert System for Food and Feed (RASFF) and traced back on MedISys and ProMED-mail. In addition, from January to March 2009, food- and feed-borne (re-)emerging hazards were monitored on MedISys and traced back on ProMED-mail and RASFF. MedISys has demonstrated to be an effective early-warning system for food- and feed-borne hazards. However, further customization is required to improve its sensitivity, in particular by increasing the number of multi-lingual categories related to food and feed items. MedISys tended to detect food- and feed-borne hazards earlier and more frequently than ProMED-mail, but the information from both systems was often complementary.     

Acrylamide removal from heated foods

The possibility to remove acrylamide from foods by exploiting its chemical physical properties was studied. Commercial biscuits and potato chips were subjected to vacuum treatments at different combinations of pressure, temperature and time. Results showed that acrylamide removal was achieved only in samples previously hydrated at water activity values higher than 0.83, and that, a maximum of acrylamide removal was obtained between 5 and 15 min of vacuum treatment at 6.67 Pa and 60 °C. By applying these process conditions, it was possible to remove 43% and 18% acrylamide from the biscuits and the potato chips, respectively. It was hypothesised that the vacuum treatment could favour acrylamide formation by promoting the decarboxylation of the Schiff base, a key intermediate of acrylamide formation. Although further research is needed to find out for each food category the process conditions that can maximise acrylamide removal while minimising its formation as well as to evaluate the effects on the sensory properties, this technology would represent a promising and alternative strategy to mitigation interventions aimed at reducing acrylamide levels in foods.     

Atmospheric pressure mass spectrometry: a new analytical chemical characterization method for dissolved organic matter in rainwater

The complex mixture of organic compounds in the atmosphere influences climate, air quality, and ecosystem processes. Atmospheric pressure electrospray ionization mass spectrometry (APESI-MS) was evaluated as a potential tool for direct measurement of the total suite of individual dissolved organic matter (DOM) compounds in rainwater. The APESI-MS response was linear to all DOM compounds of atmospheric significance examined as standard solutions. Urban precipitation samples from New Brunswick, NJ (USA) were analyzed by APESI-MS over the mass-to-charge (m/z) range 50-3,000. Over 95% of the m/z ions detected were in the low m/z range (50-500). Over 300 unique m/z ions were detected across the 11 rainwater samples indicating the complexity of the mixture of DOM in rainwater. Forty percent of the organic bases (positive mode detection) and 22% of the organic acids (negative mode) occurred in at least 6 of the 11 rainwater samples. Ions corresponding to the m/z of carboxylic acids standards (nonanedioic acid; 1,4-butanedicarboxylic acid; pentanedioic acid; hydroxybutanedioic acid; and butanedioic acid) and to reduced N standards (allylurea; caffeine; imidazole; and N-2-propenylurea) occurred in at least one of the 11 rainwater samples. Total dissolved organic carbon (DOC) estimated from the APESI-MS analysis and measured by standard DOC methods were not statistically different.     

Exposure to fine and ultrafine particles from secondhand smoke in public places before and after the smoking ban, Italy 2005

Background

A smoking ban in all indoor public places was enforced in Italy on 10 January 2005.

Methods

We compared indoor air quality before and after the smoking ban by monitoring the indoor concentrations of fine (<2.5 μm diameter, PM_2.5) and ultrafine particulate matter (<0.1 μm diameter, UFP). PM_2.5 and ultrafine particles were measured in 40 public places (14 bars, six fast food restaurants, eight restaurants, six game rooms, six pubs) in Rome, before and after the introduction of the law banning smoking (after 3 and 12 months). Measurements were taken using real time particle monitors (DustTRAK Mod. 8520 TSI; Ultra‐fine Particles Counter‐TRAK Model 8525 TSI). The PM_2.5 data were scaled using a correction equation derived from a comparison with the reference method (gravimetric measurement). The study was completed by measuring urinary cotinine, and pre‐law and post‐law enforcement among non‐smoking employees at these establishments

Results

In the post‐law period, PM_2.5 decreased significantly from a mean concentration of 119.3 μg/m³ to 38.2 μg/m³ after 3 months (p<0.005), and then to 43.3 μg/m³ a year later (p<0.01). The UFP concentrations also decreased significantly from 76 956 particles/cm³ to 38 079 particles/cm³ (p<0.0001) and then to 51 692 particles/cm³ (p<0.01). Similarly, the concentration of urinary cotinine among non‐smoking workers decreased from 17.8 ng/ml to 5.5 ng/ml (p<0.0001) and then to 3.7 ng/ml (p<0.0001).

Conclusion

The application of the smoking ban led to a considerable reduction in the exposure to indoor fine and ultrafine particles in hospitality venues, confirmed by a contemporaneous reduction of urinary cotinine.