Comparative Analysis of Antioxidative Activity of Flavonoids Using HPLC–ED and Photometric Assays

The present investigation reports on the application of a new antioxidant activity assay for the examination of flavonoids. It has been shown that the high-performance liquid chromatography with electrochemical detection (HPLC–ED) measurements allow to obtain additional information about the antioxidative properties of pure compounds by measuring their half-wave potential, the chromatographic peak height, and the product of the peak height and exponent of potential. In comparison to the classical electrochemical measurements, the HPLC–ED is characterized by a much smaller detection limit. The results were compared with the standard photometric measurement based on 1,1-diphenyl-2-picrylhydrazyl radicals. The possible antioxidant activity forecasting is also discussed.     

Effect of relative humidity and light conditions on the oxidative stability of sunflower oil blends stabilised with synthetic and natural antioxidants

The effect of relative humidity (RH) and light conditions on the oxidative stability of sunflower oil (SO) stabilised with oleoresin rosemary + ascorbyl palmitate (SOR), sage + ascorbyl palmitate (SOS) and tertiary butyl hydroquinone (SOT) was investigated. The SO without additive (SOC) served as positive control. Oil samples were subjected to 3 RH (29%, 52% and 75%) and two light conditions (dark, D and fluorescent light (600 lx), L) at 60 °C for 7 days. The oxidative stability was monitored by peroxide value (PV), conjugated diene value (CDV), free fatty acids (FFAs) and Rancimat induction period (IP). A direct relationship was noted between RH and increase in PV, CDV and FFA levels irrespective of light conditions. The detrimental effect of light on the oxidative stability was prominently noted at higher RH (75%) which followed an order: SOC > SOT > SOR > SOS. The samples stored under lower RH (29% and 52%), and dark conditions were better stabilised than light‐exposed counterparts.     

Ultrastructural and developmental toxicity of potato and tomato leaf extracts to beet armyworm,Spodoptera exigua (lepidoptera: noctuidae)

Beet Armyworm, Spodoptera exigua is a herbivorous moth and a serious pest of many economically important plants, which are used as food sources. Because of rigorous standards of food quality, usage of synthetic insecticides in crop protection, against pests, is limited. Solanaceae plant extracts may be a relatively cheap source of efficient natural insecticides that can limit usage of synthetic substances. Their biological activity is not fully known. In particular, ultrastructural studies, using transmission electron microscopy, are not usual. In the present article we describe the effects of sublethal concentrations of tomato and potato leaf extracts against S. exigua. Acute lethal effects were not observed. Both extracts exerted similar effects within midgut and fat body cells. Midgut cells were not significantly altered while fat body cells showed prominent swelling of nuclear envelope and endoplasmic reticulum, vacuolization of mitochondria and fusion of fat droplets. These changes were much more intensive within groups exposed to potato than tomato extracts at highest concentration at least. Light microscopy was used to observe and document developmental alterations of S. exigua exposed to potato and tomato leaf extracts. Potato leaf extracts significantly decreased hatching success and caused morphological malformations of imagoes. Among them, malformations of wings were the most prominent. Interestingly, these effects were not observed within populations exposed to tomato extracts at highest concentration at least.     

Structure of lean premixed hydrogen-air flames in an annular microcombustor

Self-sustainable combustion and narrow stability limits are the most critical issues in microcombustors (length scale 1 mm) as increased heat losses can lead to thermal quenching of the flame. Hydrogen is a potential fuel for microcombustion due to its high specific energy and wide flammability limits. This work focuses on the lean premixed hydrogen-air flames stabilized in a newly developed annular microcombustor. Detailed axisymmetric numerical calculations involving multistep kinetics, multicomponent mass-heat transport, conjugate heat transfer, and thermal radiation in gas and solid media are performed. It has been shown that flame stabilization occurs by preheating even though wall temperatures are higher than the autoignition temperature. Results unravel the importance of H radical reactions in the kinetics of microflames in low- and high-temperature regions of the microcombustor.     

The effect of temperature and radiation on flavonol aglycones and flavonol glycosides of kale (Brassica oleracea var. sabellica)

The winter crop kale has a complex profile of different glycosylated and acylated flavonol glycosides which may be affected by global warming. To the best of our knowledge, compound–climate relationships for flavonol aglycones and flavonol glycosides were established for the first time. The investigated 10 major flavonol glycosides responded structure-dependent in the investigated temperature range between 0 and 12 °C and the photosynthetic active radiation range between 4 and 20 mol m⁻² d⁻¹, e.g. the decrease in temperature led to an increase in sinapic acid monoacylated and diacylated quercetin glycosides, while the sinapic acid monoacylated kaempferol glycosides showed a maximum at 4.5 °C. Furthermore, the hydroxycinnamic acid residues and the different number of glucose moieties in the 7-O position affected the response of kaempferol triglucosides. Consequently, global warming would result in lower concentrations of antioxidant-relevant quercetin glycosides in winter crops, suggesting a production at e.g. higher altitudes due to lower temperature.     

Fatigue lifetime study of piezoceramic patch transducers

This study investigates the loading limits and fatigue behavior of laminar piezoceramic patch transducers, based on monolithic lead zirconate titanate (PZT) plates encapsulated in epoxy resin matrix. Testing includes tensile and compressive mechanical loading, at different temperatures, as well as electric actuation. The objective is the identification of typical failure mechanisms and suitable models for failure and lifetime analysis. In tensile loading, which is observed to have the strongest influence on the patch lifetime, the relevant failure mechanism in quasi-static and cyclic loading is found to be cracking of the PZT plate, causing degradation of the patch performance. Based on the micro-structural investigations and FE simulation, the fracture toughness of the PZT plate is analyzed and a micro-crack-propagation-based fatigue lifetime model is derived. The effect of the fragmentation of the piezoceramic plate on the patch performance is analyzed by the presented functional length concept, based on the shear lag model, yielding very good quantitative agreement.     

Numerical Analysis of Bird Impact on Glass-Reinforced Leading Edge of an Aircraft Wing

The paper describes the methodology of modeling and simulation of bird impact mechanism of GLARE laminate structures. The bird is modeled using Lagrangian concept. Explicit finite element techniques have been developed to simulate the impact mechanics. The study involves deeper understanding of impact dynamics and contact mechanics. The bird impact analysis has been carried out on typical configuration of GLARE 3/2, 4/3, 5/4, 6/5, 7/6 and 8/7. The results of stress propagation and material deformation at high strain rate have been obtained. Results from the numerical analysis are compared with experimental results, and the material is found to be capable of absorbing the impact energy. The results also show that the bird material model chosen to simulate for carrying out impact mechanics analysis is found to be capable of capturing most of the complex behavior exhibited by functional structural material GLARE.