DNA sequence analysis of a 17 kb fragment of yeast chromosome XI physically localizes the MRB1 gene and reveals eight new open reading frames,including a homologue of the KIN1/KIN2 and SNF1 protein kinases

We report in this paper the sequence of a part of chromosome XI of Saccharomyces cerevisiae. This 17 kbp nucleotide sequence represents the right half of cosmid pUKG151 and contains nine open reading frames, YKL453, 450, 449, 448, 445, 443, 442, 441 and the 5′ part of YKL440. YKL440 was previously identified as the MBR1 gene and plays a role in mitochondrial biogenesis. YKL443 is a homologue of the yeast serine-rich protein (SRP1), while YKL453 presents strong homologies with the KIN1/KIN2/SNF1 kinase family. It must be pointed out that the size of this gene is well above average for yeast.     

Zr-二十面体准晶-十次准晶伪三元系中非晶的形成

分析了块状非晶与稳定准晶结构上的异同点，选择两种稳定准晶(二十面体准晶和十次准晶)相互混合，在保持准晶二十面体基本结构单元的基础上，增加元素组成的混乱度，再添加有利于形成高配位多面体的元素Zr，用急冷方法制备出非晶合金．对此非晶合金的热力学测试表明，该非晶形成能力和稳定性与Inoue的Zr65Al7.5Cu17.5Ni10非晶相近．     

CHOOSE: Towards a metamodel for enterprise architecture in small and medium-sized enterprises

Enterprise architecture (EA) is a coherent whole of principles, methods, and models that are used in the design and realization of an enterprise’s organizational structure, business processes, information systems, and IT infrastructure. Recent research indicates the need for EA in small and medium-sized enterprises (SMEs), important drivers of the economy, as they struggle with problems related to a lack of structure and overview of their business. However, existing EA frameworks are perceived as too complex and, to date, none of the EA approaches are sufficiently adapted to the SME context. Therefore, this paper presents the CHOOSE metamodel for EA in SMEs that was developed and evaluated through action research in an SME and further refined and validated through case study research in five other SMEs. This metamodel is based on the essential dimensions of EA frameworks and is kept simple so that it may be applied in an SME context. The final CHOOSE metamodel includes only four essential concepts (i.e. goal, actor, operation, object), one for each most frequently used EA focus. As an example, an extract is included from the specific model that was created for the SME used in our action research. Finally, the CHOOSE metamodel is evaluated according to the dimensions essential in EA and the requirements for EA in an SME context.     

Première analyse des mesures de propagation oblique effectuées à Gometz-La-Ville sur le signal du satellite SIRIO 1

This paper discusses the results of measurements on the emissions of the Italian satellitesirio 1, carried out at the Gometz-la-Ville earth station during a one year period in order to assess the quality of future Earth-satellite links at frequencies above 10 GHz. The contributions of rain and snow to the measured attenuation are determined. The results are compared with those obtained by theccir prediction method. The attenuation due to a wet plane radome is evaluated. The variations of polarization decoupling as a function of attenuation are compared with those predicted by theoretical models and a method for determining the quality of the liaison when the same frequency is used on two orthogonal polarizations is proposed.     

Phenolic compounds on the pod-surface of pigeonpea,Cajanus cajan,mediate feeding behavior of Helicoverpa armigera larvae

A methanol extract of the pod surfaces of Cajanus cajan, a feeding stimulant for fifth-instar Helicoverpa armigera, was shown to contain four main phenolic compounds. Three of these were identified as isoquercitrin, quercetin, and quercetin-3-methyl ether, by comparing UV spectra and HPLC retention times with authentic standards. The fourth compound was isolated by semipreparative HPLC and determined to be 3-hydroxy-4-prenyl-5- methoxystilbene-2-carboxylic acid (stilbene) by NMR spectroscopy and mass spectrometry. Quercetin, isoquercitrin, and quercetin-3-methyl did not affect the selection-behavior of fifth-instar H. armigera. However, larvae were deterred from feeding on glass-fiber disks impregnated with the stilbene. Furthermore, larvae exposed to quercetin-3-methyl ether consumed significant amounts of both disks. In a binary-choice bioassay, a combination of quercetin-3-methyl ether and the stilbene on one disk and pure quercetin-3-methyl ether on the other disk resulted in increased consumption of both glass-fiber disks by larvae. In contrast, consumption was reduced if the combination was presented to larvae on one disk with purified stilbene on the other disk. Cajanus cajan cultivars that varied in their susceptibility to H. armigera were surveyed for the presence of the four phenolic compounds. An absence of quercetin and higher concentrations of isoquercitrin than the cultivated variety characterized pod surface extracts of pod-borer-resistant cultivars. In addition, the ratio of the stilbene to quercetin-3-methyl ether was greater in the pod-borer-resistant cultivars. These findings are discussed in relation to the identification of chemical characters that can be used for crop improvement.     

Numerical simulation of the transient hydrogen trapping process using an analytical approximation of the McNabb and Foster equation

In order to simulate hydrogen charging and discharging cycles of mechanically loaded structures, an analytical solution for the differential equation of trapping kinetics is proposed, as a generalization of the Oriani's equilibrium relationship.This solution has been implemented in the Abaqus finite element software, and validated by comparison with the one-dimensional kinetic MRE Hydrogen Isotope Inventory Processes Code (HIIPC). Last, the results of an application on a 3D structure are presented.     

Exploration of regional and global cost–supply curves of biomass energy from short-rotation crops at abandoned cropland and rest land under four IPCC SRES land-use scenarios

We explored the production cost of energy crops at abandoned agricultural land and at rest land at a regional and a global level to the year 2050 using four different land-use scenarios. The estimations were based on grid cell data on the productivity of short-rotation crops on the available land over time and assumptions regarding the capital and the labour input required to reach these productivity levels. It was concluded that large amounts of grown biomass at abandoned agricultural land and rest land, 130–270 EJ yr^?1 (about 40–70% of the present energy consumption) may be produced at costs below $2 GJ^?1 by 2050 (present lower limit of cost of coal). Interesting regions because of their low production cost and significant potentials are the Former USSR, Oceania, Eastern and Western Africa and East Asia. Such low costs presume significant land productivity improvements over time and cost reductions due to learning and capital-labour substitution. An assessment of biomass fuel cost, using the primary biomass energy costs, showed that the future costs of biomass liquid fuels may be in the same order of the present diesel production costs, although this may change in the long term. Biomass-derived electricity costs are at present slightly higher than electricity baseload costs and may directly compete with estimated future production costs of fossil fuel electricity with CO₂ sequestration. The present world electricity consumption of around 20 PWh yr^?1 may be generated in 2050 at costs below $45 MWh^?1 in A1 and B1 and below $55 MWh^?1 in A2 and B2. At costs of $60 MWh^?1, about 18 (A2) to 53 (A1) PWh yr^?1 can be produced.     

Antimicrobial activity of mustard essential oil against Escherichia coli O157:H7 and Salmonella typhi

The aim of this study was to investigate how mustard essential oil (EO) affected the cell membrane of Escherichia coli O157:H7 and Salmonella typhi. Intracellular pH and ATP concentration and the release of cell constituents were measured when mustard EO was in contact with E. coli and S. typhi at its minimal inhibitory concentration (MIC) and maximal tolerated concentration (MTC). The treatment with mustard EO affected the membrane integrity of bacteria and induced a decrease of the intracellular ATP concentration. Also, the extracellular ATP concentration increased and a reduction of the intracellular pH was observed in both bacteria. A significantly (P ? 0.05) higher release of cell constituent was observed when both bacteria cells were treated with mustard EO. Electronic microscopy observations showed that the cell membranes of both bacteria were apparently damaged by mustard EO. In conclusion, mustard EO affects the concentration of intracellular component, such as ATP in both bacteria and affects the pH suggesting that cytoplasmic membrane is involved in the antimicrobial action of mustard EO. Mustard EO can be used as an effective antimicrobial agent. We have demonstrated that mustard EO affected the cell membrane integrity, resulting in a loss of cell homeostasis.     

Neurotrophic and Immunomodulatory Lanostane Triterpenoids from Wood-Inhabiting Basidiomycota

Neurotrophins such as nerve growth factor (ngf) and brain-derived neurotrophic factor (bdnf) play important roles in the central nervous system. They are potential therapeutic drugs for the treatment of neurodegenerative diseases, including Alzheimer’s disease and Parkinson’s disease. In this study, we investigated the neurotrophic properties of triterpenes isolated from fruiting bodies of Laetiporus sulphureus and a mycelial culture of Antrodia sp. MUCL 56049. The structures of the isolated compounds were elucidated based on nuclear magnetic resonance (NMR) spectroscopy in combination with high-resolution electrospray mass spectrometry (HR-ESIMS). The secondary metabolites were tested for neurotrophin (ngf and bdnf) expression levels on human astrocytoma 1321N1 cells. Neurite outgrowth activity using rat pheochromocytoma (PC-12) cells was also determined. Twelve triterpenoids were isolated, of which several potently stimulated the expression of neurotrophic factors, namely, ngf (sulphurenic acid, 15α-dehydroxytrametenolic acid, fomefficinic acid D, and 16α-hydroxyeburicoic acid) and bdnf (sulphurenic acid and 15α-dehydroxytrametenolic acid), respectively. The triterpenes also potentiated ngf-induced neurite outgrowth in PC-12 cells. This is, to the best of our knowledge, the first report on the compound class of lanostanes in direct relation to bdnf and ngf enhancement. These compounds are widespread in medicinal mushrooms; hence, they appear promising as a starting point for the development of drugs and mycopharmaceuticals to combat neurodegenerative diseases. Interestingly, they do not show any pronounced cytotoxicity and may, therefore, be better suited for therapy than many other neurotrophic compounds that were previously reported.     

Flexible corrections of juror judgments: Implications for jury instructions.

The Flexible Correction Model (FCM, D. T. Wegener & R. E. Petty, 1997; D. T. Wegener, R. E. Petty, & M. Dunn, 1998) conceptualizes efforts at bias correction (i.e., attempts to remove influences that are perceived as illegitimate or unwanted) as guided by people's naive theories (perceptions) of the influences at work in that judgment setting. In this article, the authors present this model, discuss the general support for this model outside of courtroom judgment, and discuss a variety of implications of this model for courtroom judgment in general and for the impact of judges' instructions to juries in particular. (PsycINFO Database Record (c) 2010 APA, all rights reserved)