Multi-dimensional modelling of multiphase flow physics: high-speed nozzle and jet flows — a case study

Multi-dimensional modelling of multiphase flows has become more prevalent as computer capabilities have significantly expanded. Such analyses are necessary if the flow physics demonstrates behavior that is fundamentally different from the estimates of one-dimensional analyses. Multiphase multi-dimensional behavior may involve physical mechanisms that interact with the flow field transverse to the main fluid direction and feedback into downstream processes. Consider the physics of high-speed internal nozzle flow, downstream external jet flow and the dynamics of jet breakup. This is a prime example of a coupled problem where multi-dimensional aspects may need to be considered. This paper examines multiphase physics as an illustration of the conditions under which multi-dimensional modelling would be required. Internal nozzle flow can involve cavitation phenomena, and as the geometry becomes more abrupt or asymmetric, multi-dimensional modelling is required. High-speed simulations using our internal flow model, CAVALRY, indicate that cavitation behavior can become oscillatory as the nozzle shape is altered. This exiting internal flow emerges as a multi-dimensional external jet flow, whose downstream breakup can be noticeably influenced by the inlet conditions as well as the jet breakup mechanisms. Jet breakup models first developed for the TEXASV model are utilized in the multi-dimensional KIVA code simulations for gas–liquid flows. The simulation results suggest that similar jet breakup mechanisms are operative for a multi-fluid system. Our comparisons to particular sets of data for high-speed nozzle flow and jet breakup in a gas suggest that the approach can be extended to multiphase systems using similar concepts; i.e. TEXAS-3d.     

考虑器壁侵蚀影响的低压断路器电弧运动特性仿真及实验

以磁流体动力学(MHD)为基础建立了考虑器壁侵蚀影响的低压断路器中的电弧的数学模型.在传统的质量、动量和能量守恒方程的基础上增加了器壁材料的浓度方程,考虑了器壁材料的烧蚀对气体的热动力学特性和传输系数的影响,并且采用"视角因数法"计算到达器壁的辐射能量.利用基于有限容积法的商业软件对上述模型进行求解,得出灭弧室内温度场、浓度场、气流场的分布情况以及电压随时间的变化过程.仿真结果表明,由于灭弧室内"漩涡"的存在使得电弧后方的聚合物蒸气浓度大于电弧前方的聚合物蒸气浓度;由于产气材料的影响,电弧的最大电压增加了19.2%;产气材料对电弧运动有加速作用,由于产气材料的影响,电弧的平均运动速度增加了20.1%.实验结果验证了仿真模型的合理性.     

胶体量子点技术使太阳能转换效率达8％

加拿大多伦多大学的研究人员设计并测试了一种新型固态、稳定的光敏纳米粒子——胶体量子点技术,或将用于开发更为廉价、柔性的太阳能电池,以及更好的气体感应器、红外激光器、红外发光二极管等。     

Peptides Containing meso-Oxa-Diaminopimelic Acid as Substrates for the Cell-Shape-Determining Proteases Csd6 and Pgp2

The enzymes Csd6 and Pgp2 are peptidoglycan (PG) proteases found in the pathogenic bacteria Helicobacter pylori and Campylobacter jejuni, respectively. These enzymes are involved in the trimming of non-crosslinked PG sidechains and catalyze the cleavage of the bond between meso-diaminopimelic acid (meso-Dap) and d -alanine, thus converting a PG tetrapeptide into a PG tripeptide. They are known to be cell-shape-determining enzymes, because deletion of the corresponding genes results in mutant strains that have lost the normal helical phenotype and instead possess a straight-rod morphology. In this work, we report two approaches directed towards the synthesis of the tripeptide substrate Ac-iso-d -Glu-meso-oxa-Dap-d -Ala, which serves as a mimic of the terminus of an non-crosslinked PG tetrapeptide substrate. The isosteric analogue meso-oxa-Dap was utilized in place of meso-Dap to simplify the synthetic procedure. The more efficient synthesis involved ring opening of a peptide-embedded aziridine by a serine-based nucleophile. A branched tetrapeptide was also prepared as a mimic of the terminus of a crosslinked PG tetrapeptide. We used MS analysis to demonstrate that the tripeptide serves as a substrate for both Csd6 and Pgp2 and that the branched tetrapeptide serves as a substrate for Pgp2, albeit at a significantly slower rate.     

Assessing the Delivery of Molecules to the Mitochondrial Matrix Using Click Chemistry

Mitochondria are central to health and disease, hence there is considerable interest in developing mitochondria‐targeted therapies that require the delivery of peptides or nucleic acid oligomers. However, progress has been impeded by the lack of a measure of mitochondrial import of these molecules. Here, we address this need by quantitatively detecting molecules within the mitochondrial matrix. We used a mitochondria‐ targeted cyclooctyne (MitoOct) that accumulates several‐ hundredfold in the matrix, driven by the membrane potential. There, MitoOct reacts through click chemistry with an azide on the target molecule to form a diagnostic product that can be quantified by mass spectrometry. Because the membrane potential‐dependent MitoOct concentration in the matrix is essential for conjugation, we can now determine definitively whether a putative mitochondrion‐targeted molecule reaches the matrix. This “ClickIn” approach will facilitate development of mitochondria‐targeted therapies.     

Limited hydrolysis of soy proteins with endo- and exoproteases

Changes in the native state and functional properties of soy protein achieved by limited proteolysis of soy flour were investigated. Different enzyme-to-substrate ratios (E/S) were used to obtain low (3–5%) and medium (5–10%) degrees of hydrolysis (DH). Six protease preparations (three with predominately exopeptidase activities and three with predominately endopeptidase activities) were evaluated, and their effects on solubility, emulsification capacity, SDS-PAGE profiles, and denaturation enthalpies were characterized. Endoproteases (Multifect^® Neutral, Protex^? 6L, and Multifect^® P-3000) and exoproteases (Fungal Protease Concentrate, Experimental Fungal Protease #1, and Experimental Fungal Protease #2) yielded similar increases in soy protein solubility. The modifications to the soy peptide profile were similar for the three exoprotease mixtures at a 1% E/S ratio, whereas the extent of hydrolysis with Protex^? 6L was more pronounced than with the two other endoproteases (Multifect^® Neutral and Multifect^® P-3000). The emulsification capacity of protease-modified soy flour declined regardless of DH and enzyme type (exo- or endoprotease). After hydrolysis to >4% DH, denaturation enthalpies of glycinin and β-conglycinin decreased significantly, whereas hydrolysis to lower DH did not affect these values.     

Mitochondrial Glycerol-3-Phosphate Acyltransferase-Dependent Phospholipid Synthesis Modulates Phospholipid Mass and IL-2 Production in Jurkat T Cells

Changes in glycerophospholipid metabolism with age and disease can have a profound effect on immune cell activation and effector function. We previously demonstrated that glycerol‐3‐phosphate acyltransferase‐1, the first and rate limiting step in de novo glycerophospholipid synthesis, plays a role in modulating murine T cell function. The resultant phenotype is characterized by decreased IL‐2 production, increased propensity toward apoptosis, and altered membrane glycerophospholipid mass similar to that of an aged T cell. Since T cells in previous experiments were harvested from GPAT‐1^?/? mice, questions remained as to what extent the macro environment of the model influenced the observed cellular phenotype. Therefore, we generated and phenotypically characterized a mitochondrial glycerol‐3‐phosphate acyltransferase (GPAM) deficient Jurkat T cell. Furthermore, this line was used to probe possible mechanisms by which GPAT‐1/GPAM regulates T cell function. We report here that many of the key dysfunctional characteristics of murine GPAT‐1^?/? T cells are recapitulated in the GPAMKD Jurkat T cell. We found striking decreased IL‐2 production along with altered phospholipid mass and increased incidence of apoptosis. Since PtdOH is an indirect downstream product of GPAM, we attempted to rescue IL‐2 production with PtdOH supplementation; however, this addition did not return IL‐2 production to normal levels. Interestingly, we did find significantly decreased Zap‐70 phosphorylation following stimulation, suggesting that GPAM deficiency may alter membrane based stimulatory signaling. These data show for the first time that GPAM deficiency results in an inherent defect in Jurkat T cell function and glycerophospholipid composition and that this defect cannot be rescued by addition of exogenous PtdOH.