Experiments to Relate Acoustic Emission Energy to Fracture Energy of Concrete

Acoustic emission (AE) was used to measure energy associated with fracture of standard concrete test specimens. The goal of the work was to identify ways in which AE could be used to quantify damage in generic laboratory structures for the purpose of tuning damage models. A series of mortar and concrete specimens of different compositions were tested for fracture energy Gf, while simultaneously being monitored for acoustic emission energy release. Reasonable correlation between the two quantities was observed for fine-grained specimens, however the relationship was not as good for coarse-grained specimens. Toughening mechanisms such as friction are suggested as being responsible for the poor relationship observed in the course-grained materials. It is further suggested that AE energy release can be related to actual crack formation energy but not to friction and other internal energy dissipation or toughening mechanisms.     

Analytical capabilities of inductively coupled plasma orthogonal acceleration time-of-flight mass spectrometry (ICP-oa-TOF-MS) for multi-element analysis of food and beverages

Analytical capabilities of ICP-oa-TOF-MS for rapid, simultaneous and reliable determination of more than 50 major, trace and ultra-trace elements in different food and beverages samples (milk and dairy based products, cereals, meat, offal, sugar and sugar products, potatoes, fats, baby food samples, fruit juices, alcoholic beverages), following microwave closed vessel digestion of samples, were described. Under optimum instrumental conditions, and by using Rh as an internal standard and an external calibration method, ICP-oa-TOF-MS enables an accurate analysis, taking about one minute per a sample for all elements and isotopes of interest even for some elements such Zn, Ni, Cu, As or Co whose assay is more difficult when using conventional quadrupole instruments. In order to verify the accuracy and precision of the proposed method, 8 commercially available reference materials representing 3 major groups of food (milk and dairy based products, meat, cereals) were analysed, yielding results in agreement with certified values and the precision bellow 15%. In addition, accuracy was confirmed by spiked analytical recoveries study and accurate isotopic ratio determinations with the precision typically better than 5% with 5 s data acquisition period, also for other elements of interest whose content was not certified and different sample matrices. Limits of detection (3σ) have varied from 0.04 ng g⁻¹ for Th to 1630 ng g⁻¹ for Ca.     

Treatment of odorous sulphur compounds by chemical scrubbing with hydrogen peroxide--stabilisation of the scrubbing solution

To slow down the hydrogen peroxide decomposition in basic aqueous conditions, the addition of stabilizers and co-stabilizers in the scrubbing solution was investigated. Results found with sodium silicate (Na2SiO3) were quite promising but several problems still remained. Based on these observations, this study focused on the research of a better stabilizer. Several ways were investigated: the use of silicate solutions employed in pulp industries, the addition of co-stabilizers to sodium silicate, or the use of an another stabilizer (the poly-alpha-hydroxyacrylic acid). Experiments revealed that the poly-alpha-hydroxyacrylic acid is the best stabilizing compound.     

Regulation of adiponectin and its receptors in rat ovary by human chorionic gonadotrophin treatment and potential involvement of adiponectin in granulosa cell steroidogenesis

In mammals, adiponectin and its receptors (AdipoR1 and AdipoR2) mRNAs are expressed in various tissues. However, the cellular expression and the role of adiponectin system have never been investigated in rat ovary. Here, we report the presence of adiponectin, AdipoR1 and AdipoR2 in rat ovaries, and we have investigated its role in granulosa cells. Using RT-PCR and western blot, we show that the mRNAs and proteins for adiponectin, AdipoR1 and AdipoR2 are found in the ovaries. Immunohistochemistry localized adiponectin, AdipoR1 and AdipoR2 in theca-interstitial T-I cells, corpus luteum, oocyte and less abundantly in granulosa cells. In the KGN human granulosa cell line, adiponectin mRNA and protein were undetectable; AdipoR2 was weakly expressed, whereas AdipoR1 was clearly present. Human chorionic gonadotrophin (hCG) injection (48 h) after pregnant mare serum gonadotrophin (PMSG) injection (24 h) in immature rats increased the level of adiponectin (protein) by about threefold (P < 0.05) and those of AdipoR1 by threefold (mRNA, P < 0.05) and 1.5-fold (protein, P < 0.05) in ovary, whereas the mRNA and protein levels of AdipoR2 were unchanged. Interestingly, hCG injection (48 h) after the PMSG treatment (24 h) decreased plasma adiponectin levels and increased insulin plasma levels. In vitro in primary rat granulosa cells, human adiponectin recombinant (5 microg/ml) in the presence or absence of follicle-stimulating hormone (10(-8) M, 48 h) had no effect on the steroidogenesis. However, it increased progesterone secretion (P < 0.05) by about twofold and oestradiol production (P < 0.05) by about 1.6-fold in response to insulin-like growth factor-I (IGF-I) (10(-8) M). Furthermore, it improved IGF-I-induced IGF-I receptor-beta subunit tyrosine phosphorylation and ERK1/2 phosphorylation. In basal state, human adiponectin recombinant also increased rapidly but transiently the ERK1/2, p38 and Akt phosphorylations, whereas it increased more lately the adenosine 5'-monophosphate-activated protein kinase (AMPK) phosphorylation. Thus, AdipoR1 and AdipoR2 are regulated by hCG treatment in rat ovary and adiponectin enhances IGF-I-induced steroidogenesis in granulosa cells.     

Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation, and acquisition of developmental competence in vitro: comparison with gonadotropins

The aim of this work was to assess the FSH-stimulated expression of epidermal growth factor (EGF)-like peptides in cultured cumulus-oocyte complexes (COCs) and to find out the effect of the peptides on cumulus expansion, oocyte maturation, and acquisition of developmental competence in vitro. FSH promptly stimulated expression of amphiregulin (AREG) and epiregulin (EREG), but not betacellulin (BTC) in the cultured COCs. Expression of AREG and EREG reached maximum at 2 or 4 h after FSH addition respectively. FSH also significantly stimulated expression of expansion-related genes (PTGS2, TNFAIP6, and HAS2) in the COCs at 4 and 8 h of culture, with a significant decrease at 20 h of culture. Both AREG and EREG also increased expression of the expansion-related genes; however, the relative abundance of mRNA for each gene was much lower than in the FSH-stimulated COCs. In contrast to FSH, AREG and EREG neither stimulated expression of CYP11A1 in the COCs nor an increase in progesterone production by cumulus cells. AREG and EREG stimulated maturation of oocytes and expansion of cumulus cells, although the percentage of oocytes that had reached metaphase II was significantly lower when compared to FSH-induced maturation. Nevertheless, significantly more oocytes stimulated with AREG and/or EREG developed to blastocyst stage after parthenogenetic activation when compared to oocytes stimulated with FSH alone or combinations of FSH/LH or pregnant mares serum gonadotrophin/human chorionic gonadotrophin. We conclude that EGF-like peptides do not mimic all effects of FSH on the cultured COCs; nevertheless, they yield oocytes with superior developmental competence.     

Screening of veterinary drug residues in food by LC-MS/MS. Background and challenges

Regulatory agencies and government authorities have established maximum residue limits (MRL) in various food matrices of animal origin for supporting governments and food operators in the monitoring of veterinary drug residues in the food chain, and ultimately in the consumer’s plate. Today, about 200 veterinary drug residues from several families, mainly with antibiotic, antiparasitic or antiinflammatory activities, are regulated in a variety of food matrices such as milk, meat or egg. This article provides a review of the regulatory framework in milk and muscle including data from Codex Alimentarius, Europe, the U.S.A., Canada and China for about 220 veterinary drugs. The article also provides a comprehensive overview of the challenge for food control, and emphasizes the pivotal role of liquid chromatography-mass spectrometry (LC-MS), either in tandem with quadrupoles (LC-MS/MS) or high resolution MS (LC-HRMS), for ensuring an adequate consumer protection combined with an affordable cost. The capability of a streamlined LC-MS/MS platform for screening 152 veterinary drug residues in a broad range of raw materials and finished products is highlighted in a production line perspective. The rationale for a suite of four methods intended to achieve appropriate performance in terms of scope and sensitivity is presented. Overall, the platform encompasses one stream for the determination of 105 compounds in a run (based on acidic QuEChERS-like), plus two streams for 23 β-lactams (alkaline QuEChERS-like) and 10 tetracyclines (low-temperature partitioning), respectively, and a dedicated stream for 14 aminoglycosides (molecularly-imprinted polymer).     

In vitro and in vivo satietogenic effect of yeast extracts and control of food intake in rats

Yeast extracts, by their nutritional characteristics, have a high potential as a source of biologically active molecules and functional food ingredients. In this work, in vitro and in vivo satietogenic effect of yeast extracts was examined. The in vitro results obtained for the first time showed that a yeast extract strongly stimulated the secretion of cholecystokinin (CCK) from endocrine STC-1 cells. The effects obtained with this yeast extract were compared to those obtained with other food products known for their potential involvement in satiety (milk proteins, soybean). This yeast extract showed a stronger ability to stimulate CCK secretion than all other products tested. Lack of toxicity of this yeast extract was demonstrated through cell proliferation assays. In vivo, the tests in rats confirmed the satietogenic potential of this yeast extract, particularly in terms of food intake and weight loss, but also for hormone levels.     

Deep‐fat frying of cassava: influence of raw material properties on chip quality

Thirteen cultivars of cassava (Manihot esculenta Crantz) were used to obtain chips by deep frying slices of fresh cassava flesh in palm oil. The cultivars were representative of three different levels of four major characteristics (water, cyanide, starch and amylose content) in parenchyma. The effects of raw material composition and crop age (10 and 12 months) on mass transfer (dehydration and oil uptake), texture and colour were assessed for 1.5 mm thick chips with a final water content of 0.04 kg kg⁻¹ wet basis, corresponding to a water activity of about 0.3. Frying time varied from 70 to 90 s and oil bath temperature from 140 to 160 °C. All cultivars gave a high frying yield (>0.5 kg chips kg⁻¹ fresh cassava) and a chip fat content of between 0.23 and 0.37 kg kg⁻¹ wet basis, with the highest frying yields and lowest fat contents being obtained from roots with the lowest water content and cyanide content. The intensity of darkening reactions increased in accordance with the level of reducing sugars, while the rigidity modulus of the chips was negatively correlated with the fibre content. The other characteristics (starch, amylose and total sugar content) were either not or poorly correlated with any of the chip quality parameters studied. Cyanoglucosides were only partially eliminated during frying (over 40% retention), so cultivars with a high cyanide content gave bitter chips. For a similar composition, drying rates and cooking rates were much lower when crop age increased. This could be attributed to a structural effect characterising crop age. © 2000 Society of Chemical Industry     

On the effect of various active carbon catalysts on the behaviour of carbon gas-diffusion air electrodes: 2. Acid solutions

The electrochemical performance of carbon gas-diffusion air electrodes was studied in acid solutions. Electrodes with defined structure prepared from 11 different active carbon samples were investigated when working with air and oxygen. The catalytic activity of these samples both in acid and in alkaline solutions was compared. The extent of mass transport hindrances was estimated in terms of ΔE(i) curves. The effect of platinum and polymeric phthalocyanines as promoters on the overall electrochemical characteristics of oxygen and air electrodes was studied. In the case of oxygen electrodes at E = 700 mV (vs. RHE) 120 mA/c² were obtained with NORIT NK active carbon promoted with 2.36 mg platinum/cm² and 30 mA/cm² with P-33-active carbon promoted with 13 mg iron phthalocyanine/cm² . At low current loads the ΔE(i) curves and the slopes of the linear portion of the E vs. log i curves of oxygen electrodes reflect a different electrochemical mechanism and a different rate of mass transport for pure active carbon and active carbon promoted with the above-mentioned catalysts.