Molecular Subtyping of Invasive Breast Cancer Using a PAM50-Based Multigene Expression Test-Comparison with Molecular-Like Subtyping by Tumor Grade/Immunohistochemistry and Influence on Oncologist’s Decision on Systemic Therapy in a Real-World Setting

In intermediate risk hormone receptor (HR) positive, HER2 negative breast cancer (BC), the decision regarding adjuvant chemotherapy might be facilitated by multigene expression tests. In all, 142 intermediate risk BCs were investigated using the PAM50-based multigene expression test Prosigna® in a prospective multicentric study. In 119/142 cases, Prosigna® molecular subtyping was compared with local and two central (C1 and C6) molecular-like subtypes relying on both immunohistochemistry (IHC; HRs, HER2, Ki-67) and IHC + tumor grade (IHC+G) subtyping. According to local IHC, 35.4% were Luminal A-like and 64.6% Luminal B-like subtypes (local IHC+G subtype: 31.9% Luminal A-like; 68.1% Luminal B-like). In contrast to local and C1 subtyping, C6 classified >2/3 of cases as Luminal A-like. Pairwise agreement between Prosigna® subtyping and molecular-like subtypes was fair to moderate depending on molecular-like subtyping method and center. The best agreement was observed between Prosigna® (53.8% Luminal A; 44.5% Luminal B) and C1 surrogate subtyping (Cohen’s kappa = 0.455). Adjuvant chemotherapy was suggested to 44.2% and 88.6% of Prosigna® Luminal A and Luminal B cases, respectively. Out of all Luminal A-like cases (locally IHC/IHC+G subtyping), adjuvant chemotherapy was recommended if Prosigna® testing classified as Prosigna® Luminal A at high / intermediate risk or upgraded to Prosigna® Luminal B.     

Serum Tocopherol Levels in Healthy Workers

The serum tocopherol levels were determined on 923 workers (18–62 years of age) from the textile, chemical and metallurgical industries. Tocopherol levels varied between 0,20 and 1,86 mg per 100 ml serum, the mean value being 0,77 ± 0,01 mg per 100 ml, but in 148 subjects (16%) concentrations under 0,5 mg per 100 ml were found. For women the average was significantly (p < 0,05) lower than that for men (0,75 ± 0,02 compared to 0,79 ± 0,01) and the prevalence of levels below 0,5 mg per 100 ml greater (18,4% compared to 12,6%). Independent of sex the serum tocopherol level increased with age (from 0,59 ± 0,02 under 26 years, to 0,87 ± 0,05 mg per 100 ml for the group over 55 years, p < 0,001). By grouping the persons according to the tocopherol levels (A = up to 0,5 mg per 100 ml; B = 0,51–1,0 and C = over 1,0 mg per 100 ml) and by calculating for each group the average values of the daily fat intake (supplied especially by sunflower oil) a relationship was found between the two series of values, but overall this did not reach statistical significance.     

Influence of air drying temperature on kinetics,physicochemical properties,total phenolic content and ascorbic acid of pears

This study was conducted to evaluate quality and structural changes in parallelepipedic pieces of pears during convective drying at different air temperatures (30–70 °C).Submitted to atmospheric O₂ conditions, ascorbic acid deterioration demonstrated first-order kinetic behaviour and was found to depend on air temperature and pear moisture content. Loss of ascorbic acid content increased with increasing air temperature. Possible explanation could be the irreversible oxidative reaction occurring during drying. Phenol content degradation fitted a pseudo first-order reaction and was significantly influenced by air temperature.Variations in bulk density, shrinkage and porosity essentially depended on changes in moisture content. Porosity exhibited a nonlinear variation with respect to moisture content. Volume change showed, as expected, a linear variation with moisture content. Drying temperature significantly induced the increase of a* and b* colorimetric parameters due to non-enzymatic browning reaction, which turned the samples more reddish and yellow when the temperature rose.     

Distribution of polychlorinated biphenyls in the Newark Bay Estuary

As part of the NJ Toxics Reduction Workplan for NY/NJ Harbor, ambient water samples were collected at 15 locations along the tidal portions of the Hackensack, Passaic, Raritan, Rahway and Elizabeth Rivers, and in Newark Bay, the Arthur Kill, and Kill van Kull. A Trace Organics Platform Sampler was used to collect a total of 73 dissolved phase and 73 suspended sediment phase samples between June 2000 and May 2002. These samples were analyzed for spatial and wet versus dry weather trends in the 114 polychlorinated biphenyls (PCBs; modified USEPA Method 1668A). Mean total PCB concentrations at the sampling locations ranged between 3.45 and 56 ng/L. PCB homolog groups distribution patterns at the sampling locations are presented.     

Valorization of Beef Tallow by Lipase-Catalyzed Interesterification with High Oleic Sunflower Oil

Although beef tallow (BT) has been considered a hard low-trans fat convenient to be used in several bakery applications, it has some undesirable characteristics like fatty acid composition, crystallization behavior, graininess formation and poor plastic range. This work studied the modification of BT by blending at different percentages with high oleic sunflower oil (HOSFO) followed by the enzyme-catalyzed interesterification of the blends. The reduction in the solid fat content achieved by the simple blending was enhanced by the interesterification process, as a result of the increase in the concentration of the diunsaturated monosaturated type triacylglycerols. Interesterification strongly impacted too on the crystallization behavior of the blends, since products showed more homogeneous and regular crystals than the starting mixture. Results show that lipase catalyzed interesterification of BT with HOSFO offers a useful tool for the design of fats with adjustable physicochemical properties, improved with respect to that of the starting fats.     

A comparative study of voltage controllers for series active power filter

The series active power filter seems an ideal device to improve power quality. It allows to suppress and isolate voltage based distortion such as voltage unbalance, sag, interruption and voltage harmonics. In this paper, the determination of voltage references for series active power filter based on a robust three Phase digital Locked Loop system is analysed. Different control strategies are evaluated. In first hand, performances of two linear regulators: a polynomial controller based on a pole placement theory (called RST controller) and a Proportional Integral Derivative (PID) controller are studied and compared, in order to compensate different voltage perturbations in network. In second hand, a novel hysteresis band voltage control for a series active power filter is proposed to derive the switching signals and to improve the power quality. The study was carried on simulation via software Matlab/Simulink.