Chloroplastic glycolipids fuel aldehyde biosynthesis in the marine diatom Thalassiosira rotula

Enzymatic preparations and specialized analytical tools have shown that chloroplast-derived glycolipids are the main substrates for the biosynthetic pathway that produces antiproliferative polyunsaturated aldehydes in broken cells of the marine diatom Thalassiosira rotula. This process, which is associated with the formation of free fatty acids and lyso compounds from polar lipids but not triglycerides, is largely dependent on glycolipid hydrolytic activity, rather than phospholipase A(2) as previously suggested. Preliminary characterization of lipolytic enzymes has revealed protein bands of 40-45 kDa. Under native conditions these proteins seem to be associated with soluble aggregates that have an apparent molecular weight of approximately 200 kDa. The biochemical process, which is similar to that described in the algal-bloom forming diatom Skeletonema costatum, suggests a mechanism based on decompartmentalization and mixing of preexisting enzymes and substrates.     

Influence of ageing on the dynamic behaviour and the electrochemical characteristics of a 500 We PEMFC stack

In this study, a 500 We 19 cells Proton exchange membrane fuel cell (PEMFC) stack was aged for ∼1200 h and submitted to current steps between different operating levels. Using two different multi-channel data acquisition systems (one at 100 kHz and one at 1 Hz). the evolution with ageing of individual cells and full stack's short term (∼10 s) and medium term (∼4 min) dynamic performances was followed. Undershoots and overshoots behaviours were observed for respective current step-up and step-down. It appeared that, in studied operating conditions, the first time constant was related to the charge transfer at electrode/electrolyte interfaces. After the first “plateau”, the voltage evolution was explained by a membrane water content evolution.     

Brominated flame retardants in Belgian home-produced eggs: levels and contamination sources

The extent and the sources of contamination with brominated flame retardants (BFRs), such as polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecane (HBCD), in home-produced eggs from free-foraging chicken of Belgian private owners were investigated. Various factors, such as seasonal variability, exposure of chickens through diet (kitchen waste) and soil, and elimination of BFRs through eggs and faeces were assessed. PBDEs were more important than HBCD in terms of concentrations and detection frequency. Concentrations of PBDEs and HBCD in Belgian home-produced eggs were relatively low and comparable with reported levels from other European countries and the US. The concentrations of PBDEs (sum of 13 congeners, including BDE 209) ranged between not detected and 32 ng/g lipid weight (lw), with medians of 3.0 and < 2.0 ng/g lw for the autumn 2006 and spring 2007 campaigns, respectively. When present, BDE 209 was the major PBDE congener (45% of sum PBDEs). When BDE 209 was not detected, the PBDE profile was composed of PentaBDE (BDE 99 and BDE 47), with, in some cases, higher contribution of OctaBDE (BDE 183 and BDE 153). HBCD was also detected (< 0.4 and 2.9 ng/g lw for the autumn 2006 and spring 2007 campaigns, respectively), but at lower detection frequency. The highest HBCD value was 62 ng/g lw. The similarity between profiles and seasonal variations in the concentrations of BFRs in soil and eggs indicate that soil is an important source, but not the sole source, for eggs laid by free-foraging chicken. The contamination of eggs with PBDEs and HBCD appears to be of low concern for public health and the contribution of eggs to the total daily intake of PBDEs appears to be limited (10% for chicken owners and 5% for the average Belgian consumer).     

Distribution of polychlorinated biphenyls in the Newark Bay Estuary

As part of the NJ Toxics Reduction Workplan for NY/NJ Harbor, ambient water samples were collected at 15 locations along the tidal portions of the Hackensack, Passaic, Raritan, Rahway and Elizabeth Rivers, and in Newark Bay, the Arthur Kill, and Kill van Kull. A Trace Organics Platform Sampler was used to collect a total of 73 dissolved phase and 73 suspended sediment phase samples between June 2000 and May 2002. These samples were analyzed for spatial and wet versus dry weather trends in the 114 polychlorinated biphenyls (PCBs; modified USEPA Method 1668A). Mean total PCB concentrations at the sampling locations ranged between 3.45 and 56 ng/L. PCB homolog groups distribution patterns at the sampling locations are presented.     

Discovery and Pharmacophore Mapping of a Low-Nanomolar Inhibitor of P. falciparum Growth

The treatment of malaria, the most common parasitic disease worldwide and the third deadliest infection after HIV and tuberculosis, is currently compromised by the dramatic increase and diffusion of drug resistance among the various species of Plasmodium, especially P. falciparum (Pf). In this view, the development of new antiplasmodial agents that are able to act via innovative mechanisms of action, is crucial to ensure efficacious antimalarial treatments. In one of our previous communications, we described a novel class of compounds endowed with high antiplasmodial activity, characterized by a pharmacophore never described before as antiplasmodial and identified by their 4,4’-oxybisbenzoyl amide cores. Here, through a detailed structure-activity relationship (SAR) study, we thoroughly investigated the chemical features of the reported scaffolds and successfully built a novel antiplasmodial agent active on both chloroquine (CQ)-sensitive and CQ-resistant Pf strains in the low nanomolar range, without displaying cross-resistance. Moreover, we conducted an in silico pharmacophore mapping.     

Valorization of Beef Tallow by Lipase-Catalyzed Interesterification with High Oleic Sunflower Oil

Although beef tallow (BT) has been considered a hard low-trans fat convenient to be used in several bakery applications, it has some undesirable characteristics like fatty acid composition, crystallization behavior, graininess formation and poor plastic range. This work studied the modification of BT by blending at different percentages with high oleic sunflower oil (HOSFO) followed by the enzyme-catalyzed interesterification of the blends. The reduction in the solid fat content achieved by the simple blending was enhanced by the interesterification process, as a result of the increase in the concentration of the diunsaturated monosaturated type triacylglycerols. Interesterification strongly impacted too on the crystallization behavior of the blends, since products showed more homogeneous and regular crystals than the starting mixture. Results show that lipase catalyzed interesterification of BT with HOSFO offers a useful tool for the design of fats with adjustable physicochemical properties, improved with respect to that of the starting fats.     

Apron design for protecting double‐skin façade fires

Although double‐skin façade (DSF) is an environmental‐friendly architectural feature, its fire behaviour is a deep concern. The interior glass system including the glass pane, metal frame and associated accessories will be hotter than the exterior glass system as demonstrated by earlier studies. The glass pane above the fire room will be broken to spread flame into the upper compartment. Aprons are proposed to protect the air cavity of DSF in a way similar to those outside a single‐skin façade. In this paper, the effect of aprons in protecting against fire spread from an underlying compartment to the compartments above by preventing glass breakage of the inner glass pane was studied. Fire and smoke from a post‐flashover room fire adjacent to the DSF would be trapped in the air cavity between the two glass panes. Spreading of hot gases with different apron widths was studied by numerical simulations with CFD first. Fire environment with and without breaking the apron immediately above the fire room was studied. Full‐scale burning tests on part of an experimental DSF rig were then carried out to demonstrate the performance of horizontal apron in the DSF rig of 6 m tall and air cavity depth of 2 m with different apron widths. All demonstrated that providing apron is appropriate in protecting DSF fires. Copyright © 2014 John Wiley & Sons, Ltd.     

Prediction of Cloud Ranking in a Hyperconverged Cloud Ecosystem Using Machine Learning

Cloud computing is becoming popular technology due to its functional properties and variety of customer-oriented services over the Internet. The design of reliable and high-quality cloud applications requires a strong Quality of Service QoS parameter metric. In a hyperconverged cloud ecosystem environment, building high-reliability cloud applications is a challenging job. The selection of cloud services is based on the QoS parameters that play essential roles in optimizing and improving cloud rankings. The emergence of cloud computing is significantly reshaping the digital ecosystem, and the numerous services offered by cloud service providers are playing a vital role in this transformation. Hyperconverged software-based unified utilities combine storage virtualization, compute virtualization, and network virtualization. The availability of the latter has also raised the demand for QoS. Due to the diversity of services, the respective quality parameters are also in abundance and need a carefully designed mechanism to compare and identify the critical, common, and impactful parameters. It is also necessary to reconsider the market needs in terms of service requirements and the QoS provided by various CSPs. This research provides a machine learning-based mechanism to monitor the QoS in a hyperconverged environment with three core service parameters: service quality, downtime of servers, and outage of cloud services.     

Trace elements in home-produced eggs in Belgium: Levels and spatiotemporal distribution

The purpose of this study was to evaluate the levels of arsenic, cadmium, lead, copper and zinc in home-produced eggs, soils and kitchen waste samples of private chicken owners in Belgium, and to determine spatiotemporal differences in trace element contents in eggs. Eggs were sampled in all provinces of Belgium in autumn 2006 and spring 2007. A total number of 59 private chicken owners participated in the study. Trace elements were determined by inductively coupled plasma-mass spectrometry except for mercury, which was determined by atomic absorption of mercury vapour. The mean fresh weight concentrations in eggs in autumn and spring respectively were < 8.0 and < 8.0 µg/kg for arsenic, 0.5 and < 0.5 µg/kg for cadmium, 116 and 74 µg/kg for lead, 0.43 and 0.52 mg/kg for copper, 20.3 and 19.2 mg/kg for zinc, and 3.15 and 4.44 µg/kg for mercury. Analysis of variance determined significant differences in some trace element concentrations in eggs among seasons and regions in Belgium. Average concentrations of arsenic, cadmium and mercury corresponded well with values measured in other countries, while copper and zinc concentrations were within the same order of magnitude as in other countries. Average lead concentrations were high compared to concentrations in eggs from other countries and correlated well with lead concentrations in soil, indicating that the soil is an important source. Other sources of trace elements in eggs might be home-grown vegetables and forage (grass and herbs), and indirectly, air pollution.     

Quantum-inspired design of resilient substitution boxes: From coding to hardware implementation

S-boxes constitute a cornerstone component in symmetric-key cryptographic algorithms, such as DES and AES encryption systems. In block ciphers, they are typically used to obscure the relationship between the plaintext and the ciphertext. Non-linear and non-correlated S-boxes are the most secure against linear and differential cryptanalysis. In this paper, we focus on a twofold objective: first, we evolve regular S-boxes with high non-linearity and low auto-correlation properties; then automatically generate evolvable hardware for the obtained S-box. Targeting the former, we use a quantum-inspired evolutionary algorithm to optimize regularity, non-linearity and auto-correlation, which constitute the three main desired properties in resilient S-boxes. Pursuing the latter, we exploit the same algorithm to automatically generate the evolvable hardware designs of substitution boxes that minimize hardware space and encryption/decryption time, which form the two main hardware characteristics. We compare our results against existing and well-known designs, which were produced by using conventional methods as well as through genetic algorithm. We will show that our approach provides higher quality S-boxes coding as well as circuits.