Synthesis,characterization and use of iron oxide nano particles for antibacterial activity

In this decade, the use of nano particles (NPs) against bacterial growth is increasing day by day due to remarkable alternative properties compared to molecular antibiotics. Thus, the use of iron oxide nanoparticles (IONPs) has proven one of the most important transition metals oxide‐based remedy in nanotechnological advances and biological applications due to enriched biocompatibility of iron. In this study synthesis of IONPs was carried out via co‐precipitation method. The crystallographic morphology of the synthesized particles was studied via X‐ray diffraction which revealed cubic structure of the particles, whereas, the spinal shaped morphology of the prepared NPs was confirmed from scanning electron microscopy. Likewise, the presence of the major elements in the sample was determined through energy dispersive X‐ray analysis characterization. Bactericidal effect of the NPs was assessed at pre‐defined concentrations (50 and 100 μg/ml) against Gram +ve bacteria Staphylococcus aureus, Gram ?ve bacteria Shigella dysentry and Escherichia coli . Bacterial strains, which demonstrate the potential of NPs. The purpose of this study was assessing the structure of the synthesized NPs for protective effect against harmful bacterial activity.     

Systematic approach to the correct identification of Asplenium dalhousiae (Aspleniaceae) with their medicinal uses

In present study, multiple microscope techniques were used for the systematics identification of the species Asplenium dalhousiae. The plant was collected from different phytogeographical and its natural habitat of Pakistan, where it shows higher diversity. Morphology, foliar epidermal anatomy, and spore morphological characters of the species were studied in detailed using multiple microscopic techniques through light microscopy (LM) and scanning electron microscopy (SEM). LM and SEM were used for the systematics identification of the species. Traditionally, the species is used in the ailment of many diseases, so the spore morphology, anatomical features, and morphological characters are relevant to describe the species taxonomy. The importance of multiple methods of taxonomic study (e.g., documentation and morphological characteristics) for characterizing herbs are important step in systematic certification to maintain the efficacy of herbal medicines. The aim of the present study is to examine the morphological, anatomical, and spore morphology of the species A. dalhousiae in more detailed for the correct taxonomic identification and their medicinal validation from Pakistan.     

Foliar and stem epidermal anatomy of the tribe Cynoglosseae (Boraginaceae) and their taxonomic significance

Foliar and stem epidermal anatomical features of the tribe Cynoglosseae have been studied in detail for the taxonomic identification using light microscopy (LM) and scanning electron microscopic (SEM) techniques. A comparative study was conducted on different plant parts (leaf and stem epidermal anatomy) of 25 species belonging to eight genera of the tribe Cynoglosseae (Boraginaceae) collected from different phytogeographical regions of Iran for the first time. Different qualitative and quantitative characteristics were observed in detail using LM and SEM. Results showed that although generally the stem and leaf anatomical traits were similar, but some diagnostic features were examined for distinguishing the closely related genera in the tribe. The ratio of cortex/diameter of stem and phloem/xylem, the average row number of collenchyma, palisade and spongy cells, structure of trichomes, type of indumentum and palisade arrangement were found taxonomically important. The anatomical characters were statistically analyzed using cluster analysis and principal component analysis. The study found that stem and leaf eccentrics are variable in the genus but constant within species of the same genus. Most species had typical isobilateral leaves, but some showed an incipient dorsoventrally symmetry with a layer of abaxial palisade tissue. Eglandular trichomes were observed found in all the studied species, which were recognized based on structure and function. In present study some novel characters have been observed which are of great interest to the taxonomist for the correct identification some genera delimitations. The characters studied here are of less taxonomic value and delimitating at species level.     

Taxonomic study of subfamily Nepetoideae (Lamiaceae) by polynomorphological approach

The present study is insight into pollen morphology for characterizing species and their utility in the taxonomic separation of certain taxa of subfamily Nepetoideae (Lamiaceae) from Pakistan. The pollen micromorphology of 11 species of the Nepetoideae was analyzed and documented using light microscopy and scanning electron microscopy (SEM) for both qualitative and quantitative characteristics. Most species have hexazonocolpate pollen grains but trizonocolpate and tetrazonocolpate pollen with circular and oval amb were also rarely observed in Mentha spicata. The basic pollen shape in most of the studied species was subspheroidal but prolate grains were also observed in M. spicata, S. coccinea, and S. plebeia. The exine sculpturing of Nepetoideae pollen was taxonomically very informative particularly at subfamily level. Observations of exine sculpturing with SEM revealed various types of pollen grains: reticulate, bireticulate, microreticulate, perforate, aerolate, and gammate. The bireticulate type further subdivided into three subtypes based on the number of secondary lumina in each primary lumen and is characterized by varying characteristics of the secondary reticulum and primary muri. A significant variation was observed in colpus surface ornamentation. The maximum polar diameter was found in O. americanum (58 ± 5.8 μm) and the maximum equatorial diameter observed in O. basilicum (50.25 ± 1.37 μm). Pollen features of the studied species were discussed and compared based on the current taxonomical concepts. The results showed that pollen traits of the subfamily Nepetoideae was found significant to classify the taxa. Furthermore, pollen features provide additional evidence to distinguish macromorphologically similar taxa from each other.     

Taxonomic importance of spore morphology in Thelypteridaceae from Northern Pakistan

Spore morphology of Thelypteridaceae species growing in Malakand Division, Northern Pakistan, was studied using both light microscopy and scanning electron microscopy. The taxa are Christella dentata and Glaphyropteridopsis erubescens in the subfamily Thelypteridoideae, and Phegopteris connectilis, Pseudophegopteris pyrrhorhachis, and Pseudophegopteris levingei in the subfamily Phegopteridoideae. The studied species exhibit differences in spore size, exospore thickness, color, and ornamentation. Spores of the studied species are monolete and medium‐sized, and shape is ellipsoidal in both polar and equatorial views. The average measurement of the polar diameter ranges from 27 μm to 31 μm, whereas in the equatorial direction it varied from 20 μm to 40 μm. The exospore thickness ranges from 1.2 μm to 2.4 μm. Reticulate, laevigate with microgranules, cristate, and coarsely echinate surface ornamentation are observed among the species. Multivariate analysis including unweighted pair group method with arithmetic mean and principal component analysis was used for the grouping and discrimination of species and genera.     

Optimization of cutting parameters in micro end milling operations in dry cutting condition using genetic algorithms

Micro end milling with fine grained carbide end mills is an economical way to machine small and medium lots of micro components. Considering the sensitivity of the slender end mills which are very costly compared to conventional end mills, it is imperative that the machining parameters are chosen appropriately so as to ensure maximum tool life and minimum possible production cost without violating any of the imposed constraints. Unlike in conventional end milling operations the tool life in micro end milling operations increases with axial depth of cut till it equals the tool diameter and this makes it even difficult to ascertain the appropriate depth of cut to machine a specific component. In this paper the influence of depth of cut on tool life is illustrated and depth of cut is also considered as one of the decision variables in the optimization problem. More over in this paper Genetic Algorithms (GA) based on natural laws of evolution is used to optimize the cutting parameters. Finally a test case is presented to give a clear picture of the application of the optimization algorithm.     

Systematics study through scanning electron microscopy; a tool for the authentication of herbal drug Mentha suaveolens Ehrh

In all over the world, herbal drugs are usually adulterated with similar species or varieties due to incorrect identification. Most of herbal products devoid purity and quality, therefore an attempt was carried out to identify plant species and authenticate its herbal drug products from Mentha suaveolens. Microscopy tools provide an excellent platform to identify plants at species level. In this study, microscopic and pharmacokinetic parameters of M. suaveolens were observed. Plant species were collected from high diverse areas of Northern Pakistan. Macro and micro‐morphology including palynology and anatomical features were analyzed to study M. suaveolens. Species characteristics were studied, while implementing microscopic techniques for the delimitation and identification of the species. Traditionally Mentha species are used to cure several diseases that is, digestive disorders, respiratory disorders. Micromorphology (stem, leaves, flowers structure, length etc.), palynology (shape, size of pollen etc.), and anatomical characters (types of stomata, epidermal cell shape, and trichomes) were studied. Micromorphology and anatomical characters were of great interest and significance to discuss the taxonomy of the species. Taxonomic characters were studied to characterize and authenticate the species. The aim of the present study is to observe in detail the taxonomic identification of the species in term of morphology, palynology, and foliar epidermal anatomy for the correct identification along with their medicinal uses in the area.     

THE FLOW BEHAVIOR OF SUBMICRON α-Al2O3 AQUEOUS SUSPENSIONS

The viscosity of aqueous, charged a-Al₂O₃ suspensions with and without added polyelectrolytes (polyacrylic acid (PAA 1800)) is investigated over a wide range of volume fractions and shear rates. The Carreau-Yasuda model is used for both cases for examining the shear-rate dependence; for the electrostatically-stabilized suspensions the Krieger-Dougherty and Quemada models are used to determine the maximum packing fraction. The latter analysis shows that the suspension behavior changes from liquid-like to solid-like at a volume fraction of 0.71 in the low-shear limit and at 0.89 at the high-shear limit (because of the polydispersity of the suspension). The results with polyelectrolytes indicate that the dosage of the polymer plays an important role in the viscosity of suspensions and that there is an optimum dosage of polyelectrolyte that must be added to reduce the viscosity at high volume fractions of solids.     

Structural performance of steel plate shear walls with trapezoidal corrugations and centrally-placed square perforations

Steel plate shear walls (SPSWs) are an efficient lateral force-resisting system, and can be designed with corrugated and/or perforated infill plates, depending on structural considerations, architectural requirements, and service design. This paper presents a study on the structural performance of SPSWs with horizontal trapezoidal corrugations and centrally-placed square perforations under monotonic loading. Finite element models were developed for assessment of the buckling stability, stiffness, strength, and ductility performances of the shear walls. To this end, parametric studies were performed by considering the web-plate corrugation angle, thickness, and size of opening as the varying parameters in the nonlinear pushover analyses. It was found that the design of the boundary frame members can be effective in minimizing the deformations imposed by infill plates, providing system ductility, and developing lateral load resistance through stable development of diagonal tension-field action in the web plate. The effects of introducing web-plate perforations, and increasing the size of the opening, on the structural performance were also investigated. Proper design and detailing of the SPSW, along with optimal selection of the web-plate geometrical and corrugation parameters, can ensure desirable structural behavior and seismic performance for such lateral force-resisting systems.     

Characterisation of sol–gel method synthesised MgZnFe2 O4 nanoparticles and its cytotoxic effects on breast cancer cell line,MDA MB‐231 in vitro

In this study, nanocrystalline magnesium zinc ferrite nanoparticles were successfully prepared by a simple sol–gel method using copper nitrate and ferric nitrate as raw materials. The calcined samples were characterised by differential thermal analysis/thermogravimetric analysis, Fourier transform infrared spectroscopy and X‐ray diffraction. Transmission electron microscopy revealed that the average particle size of the calcined sample was in a range of 17–41 nm with an average of 29 nm and has spherical size. A cytotoxicity test was performed on human breast cancer cells (MDA MB‐231) and (MCF‐7) at various concentrations starting from (0 µg/ml) to (800 µg/ml). The sample possessed a mild toxic effect toward MDA MB‐231 and MCF‐7 after being examined with MTT (3‐[4, 5‐dimethylthiazol‐2‐yl]‐2, 5 diphenyltetrazolium bromide) assay for up to 72 h of incubation. Higher reduction of cells viability was observed as the concentration of sample was increased in MDA MB‐231 cell line than in MCF‐7. Therefore, further cytotoxicity tests were performed on MDA MB‐231 cell line.Inspec keywords: sol‐gel processing, nanoparticles, nanofabrication, magnesium compounds, zinc compounds, toxicology, biological organs, cancer, cellular biophysics, nanomedicine, calcination, differential thermal analysis, Fourier transform infrared spectra, X‐ray diffraction, transmission electron microscopy, particle size, organic compoundsOther keywords: sol‐gel method, cytotoxic effects, breast cancer cell line, MDA MB‐231 in vitro, nanocrystalline magnesium zinc ferrite nanoparticles, copper nitrate, ferric nitrate, raw materials, calcined samples, differential thermal analysis, thermogravimetric analysis, Fourier transform infrared spectroscopy, X‐ray diffraction, transmission electron microscopy, average particle size, cytotoxicity testing, human breast cancer cells, mild toxic effect, 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyltetrazolium bromide) assay, cell viability, MCF‐7, MDA MB‐231 cell line, size 17 nm to 41 nm