Size exclusion chromatography to characterize DOC removal in drinking water treatment

A full-scale (110 ML/d) potable water treatment plant (WTP) based on the MIEX process, an innovative new process based on a strong base anion-exchange resin with magnetic properties, has been operating in Perth Western Australia since 2001. This plant has been configured so that a combined MIEX-coagulation (MIEX-C) process can be operated in parallel with a conventional enhanced coagulation (EC) process, allowing comparison of the performance of the two processes. Here, we report the use of size exclusion chromatography (SEC) to compare the removal of different apparent molecular weight (AMW) fractions of DOC by the two processes. Water was sampled from five key locations within the WTP, and SEC was carried out using three different on-line detector systems, DOC-specific detection, UV absorbance detection at lambda = 254 nm, and fluorescence detection (lambda(ex) = 282 nm; lambda(em) = 353 nm). This approach provided information on the chemical nature of the DOC comprising the various AMW fractions. The study showed that the MIEX-C process outperformed the EC process with greater removal of DOC in each of the eight separate AMW fractions identified. While EC preferentially removed the fractions of highest AMW, and those exhibiting the greatest aromatic (humic) character, MIEX-C removed DOC across all AMW fractions and did not appear to discriminate as strongly on the basis of differences in aromatic character or AMW. The results demonstrate the benefits of combining these complementary treatment processes. The study also demonstrates the utility of SEC coupled with multiple detection systems in determining the characteristics of various AMW components of DOC.     

Resistance of European and hybrid aspen wood against two brown-rot fungi

The decay resistance of European (Populus tremula L.) and hybrid (P. tremula x tremuloides) aspen wood against brown-rot fungi was investigated after three different treatments, i.e. conventional drying, press drying and heat treatment. For both aspen species, the mass loss after exposure to Gloeophyllum trabeum was higher than after exposure to Coniophora puteana, regardless of the wood treatment. Conventionally and press dried aspen wood had similar mass losses. However, heat treatment appears to be an effective method to improve the decay resistance of aspen wood, reducing the mass loss by about 30% compared with conventional and press drying. As a function of mass loss, wood exposed to G. trabeum had lower moisture content than wood exposed to C. puteana. This is thought to be due to differences in the degradation pattern between the two brown-rot fungi. On the other hand, European aspen appears to be slightly more resistant to decay than hybrid aspen.     

Inhibition of Ran guanosine triphosphatase-dependent nuclear transport by the matrix protein of vesicular stomatitis virus

Transport of macromolecules into and out of nuclei, essential steps in gene expression, are potential points of control. The matrix protein (M protein) of vesicular stomatitis virus (VSV) was shown to block transport of RNAs and proteins between the nucleus and cytoplasm of Xenopus laevis oocytes. The pattern of inhibition indicated that M protein interfered with transport that is dependent on the ras-like nuclear guanosine triphosphatase (GTPase) Ran-TC4 and its associated factors. This inhibition of nuclear transport by M protein explains several observations about the effects of VSV infection on host cell gene expression and suggests that RNA export is closely coupled to protein import.     

Characterization of DOM as a function of MW by fluorescence EEM and HPLC-SEC using UVA,DOC, and fluorescence detection

To investigate the composition of dissolved organic matter (DOM) as a function of apparent molecular weight (MW) by rapid analytical methods, high performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) was conducted with sequential on-line detectors consisting of UV, fluorescence, and quantitative DOC measurement. Fluorescence excitation-emission matrix (EEM) spectrophotometry was used to select wavelengths for the HPSEC on-line fluorescence system. The chosen peak maxima locations of excitation-emission wavelengths were 278-353 nm for protein-like substances and 337-423 nm for fulvic-like substances based on an analysis of EEM spectra for various samples and reference materials. This system provides quantitative and qualitative information on the specific MW components of DOM, including proportion of DOC (by DOC measurement), aromaticity (by comparison of UV and DOC measurements), and chemical properties (by fluorescence measurement). It further allows determination of organic matter characteristics (e.g., fulvic-like, protein-like, and polysaccharide-like substances) as a function of MW. Three types of samples (Irvine Ranch ground water (IRWD-GW), Barr Lake surface water (BL-SW), and Hawaii wastewater secondary effluent) were analyzed by the HPSEC-UVA-fluorescence-DOC system. These results were compared with fluorescence EEM for samples fractionated by HPLC-SEC. The DOM fraction in the high apparent MW range (over 10,000g/mol) consisted of polysaccharide-like substances for IRWD-GW and a mixture of polysaccharide-like/protein-like substances for BL-SW and wastewater secondary effluent. Minimal amounts of fulvic-like substances were found in the wastewater secondary effluent sample. The DOM fractions in a medium apparent MW range (5000-1000 g/M) showed higher aromaticity (fulvic in character) than any other fractions for all samples. For the DOM fraction in the low apparent MW range (below 680 g/M), additional aliphatic organic matter was found in IRWD-GW, while BL-SW contained protein-like processes. DOM plays an important role in drinking water and wastewater treatment processes. An enhanced HPSEC technique with multiple on-line detectors enables a better understanding of quantitative and qualitative DOM properties and can help to design and optimize water/wastewater treatment facilities.     

Synthesis of Polyurethane Foam Considering Mixture Blowing Agents for Application to Cryogenic Environments

Polyurethane foams, which are among the most widely used insulating materials, are generally fabricated by the chemical reaction between isocyanate and polyol‐containing chemical additives and blowing agents. Recently, many kinds of blowing agents have been used for the fabrication process of polyurethane foam, such as hydrochlorofluorocarbons (HCFCs) and chlorofluorocarbons (CFCs). However, issues have continuously arisen regarding the destruction of the ozone layer due to these compounds. In the present study, polyurethane foams are manufactured using a mixture of water blowing agents and hydrofluorocarbons (HFC‐365mfc). These samples are subjected to mechanical tests to investigate the effects of the blowing agents on the mechanical properties of the polyurethane foam within a temperature range of 25 to ?163 °C. In addition, thermal and microstructural investigations are conducted depending on the content of the HFC‐365mfc. From the scanning electron microscopy observations, the sizes of the structure cells and the windows are found to decrease with the increase in HFC‐365mfc content. In addition, from the Fourier transform‐infrared spectroscopy observations, the chemical bonds that affect mechanical performance are found to be more distributed with the increase in HFC‐365mfc content.     

Automated tracking of liquid velocities in a refractive index matched porous medium

Abstract

Particle tracking velocimetry is applied to flow inside a porous column at Reynolds number Re = 28. The column is composed of refractive‐index‐matched solid and liquid materials, allowing seeding particles to be tracked in a laser‐illuminated axial slice. To complement earlier results acquired for 7 mm spheres, we conduct new experiments with larger 12 mm spheres. By improving the image acquisition and analysis, we are able to process the new experiments using fully automated algorithms instead of manual tracking. As a result, greater vector yields, more accurate velocity data, and a more complete spatial coverage are achieved.     

A comparison of sitting posture adaptations of pregnant and non-pregnant females

The postural differences between seated pregnant and non-pregnant subjects were studied, and the effects of various sitting workstation designs were assessed. Five pregnant and five non-pregnant subjects participated in this study. The experimental variables were the table-chair-vertical-distance (35 cm and 30 cm) and the seat-inclination-angle (0°, +10° and +20°). The seated working postures of the last 10 min of a 50-min sitting session were videotaped and analyzed using a 2D motion analysis system. Postural differences were observed between the pregnant and non-pregnant groups which may be attributed to the changes and/or mechanisms associated with pregnancy. The increased size of the abdomen requires more room in front of the body; in the pregnant subjects this was obtained by holding the upper-arm further from the body, inclining the upper trunk more anterior, and extending the hip more. A seat with a sloping angle of 10° was found to reduce the biomechanical loading on the lower back and in subjective ratings from both groups, was preferred over the other seat angles.

Relevance to industry

Pregnant women make up a small but significant part of the workforce. Changes in body form and working posture due to pregnancy change the effective working envelope and increase the biomechanical loads on the musculoskeletal system resulting in an increase in the risk of lower-back pain. This study examined the posture adaptations and the effects of workstation design on pregnant and non-pregnant subjects.     

Virtual Reality Application for Direct-Drive Robot with Force Feedback

Most of the current virtual reality systems use geometric models and non-sensing robots for simulation, which cannot provide an insight into the real operation of the task. In this paper, we present a new type of telerobotics based on the haptic virtual reality (VR) approach for simulating hitting a ball with force feedback attached to a human arm. The main components of the approach include a user interface, simulation, and a robot control scheme. The user interface is implemented as a combination of a virtual paddle and a graphic interface. The human operator manipulates a direct-drive type handler in the simulated environment, and the environment is simulated by the haptic virtual system that enables the operator to feel the actual force feedback from the virtual environment just as she/he would from the real environment. The haptic virtual system integrates the dynamics of the direct-drive active paddle and the virtual environment, whereas the paddle actuator consists of the dynamics of the paddle and the operator on the physical side. The control scheme employs a dynamic controller that is designed considering the force from the operator imposed on the paddle, and the force from the environment imposed on the paddle and feedback to the human arm. Experiments in the virtual environment on hitting a virtual ball system are used to validate the theoretical developments. ID="A1"Correspondance and offprint requests to: M.-G. Her, Graduate Institute of Mechanical Engineering, Tatung University, 40 Chung-Sang North Road, 3rd Secto, Taipei, 10451, Taiwan. E-mail: kshsu@kyit.edu.tw