A 65-nm SoC Embedded 6T-SRAM Designed for Manufacturability With Read and Write Operation Stabilizing Circuits

In the sub-100-nm CMOS generation, a large local Vth variability degrades the 6T-SRAM cell stability, so that we have to consider this local variability as well as the global variability to achieve high-yield SRAM products. Therefore, we need to employ some assist circuits to expand the SRAM operating margin. We propose a variability-tolerant 6T-SRAM cell layout and new circuit techniques to improve both the read and the write operating margins in the presence of a large Vth variability. By applying these circuit techniques to a 0.494-mum² SRAM cell with a beta ratio of 1, which is an extremely small cell size, we can achieve a high-yield 8M-SRAM for a wide range of Vth values using a 65-nm low stand-by power (LSTP) CMOS technology     

Occurrence of 2- and 3-hydroxy fatty acids in high concentrations in the extractable and bound lipids of Flavobacterium meningosepticum and Flavobacterium IIb

The major hydroxy fatty acids of cellular lipids in Flavobacterium meningosepticum and Flavobacterium sp. King's group UUb were identified as 2-hydroxy 13-methyltetradecanoic, 3-hydroxy 13-methyltetradecanoic, 3-hydroxy palmitic, and 3-hydroxy 15-methylhexadecanoic acids using gas chromatography-mass spectrometry and GC-mass fragmentography. The concentration of these hydroxy fatty acids comprised up to 30-40% of the total extractable and 20-30% of the bound lipid fatty acids, respectively. From the stability for mild alkaline hydrolysis, 2-hydroxy fatty acids seemed to be attached with ester linkage, and 3-hydroxy fatty acids with amide linkage.     

Semiautomated enzyme immunoassay of thyrotropin as a mass screening test for neonatal hypothyroidism

A sensitive, simple, and rapid semiautomated sandwich enzyme immunoassay (EIA) was developed for measuring thyrotropin in dried blood samples on filter paper for use in screening for neonatal hypothyroidism. Good correlation was found between values for thyrotropin determined by this method and those determined by radioimmunoassay (RIA) (r=0.94). In pilot tests on 17,160 newborn infants in the general population, five cases of primary hypothyroidism were detected by both EIA and RIA. The recall rate was slightly highter in EIA than in RIA.     

Motion control of a magnetically levitated microrobot using magnetic flux measurement

Recent advancements in micro/nano domain technologies have led to a renewed interest in ultra-high resolution magnetic-based actuation mechanisms. This paper deals with the development of a novel research-made magnetic microrobotic station (MMS) with promising potential in biological/biomedical applications. The MMS consists of two separate basic components: a magnetic drive unit and a microrobot. The magnetic drive unit produces and regulates the magnetic field for non-contact propelling of the microrobot in an enclosed environment. Our previous research findings have reported that the MMS should be equipped with high accuracy laser sensors for the position determination of the microrobot in the workspace. However, the laser positioning techniques can be used only in highly transparent environments. This paper seeks to address microrobot position estimation in non-transparent environments. A novel technique based on real-time magnetic flux measurement has been proposed for position estimation of the microrobot in the case of the laser beam blockage. A combination of Hall-effect sensors is employed in the structure of the magnetic drive unit to find the microrobot’s position using the produced magnetic flux. The most effective installation position for the Hall-effect sensors has been determined based on the accuracy sensitivity of experimental measurements. We derived a mathematical function which relates Hall-effect sensors’ voltage output and the position of the microrobot. The motion control capability of the Hall-effect-based positioning method is experimentally verified in the horizontal axis, and it was demonstrated that the microrobot can be operated in most of the workspace range with an accuracy of 0.3?mm as the root-mean-square of the position error.     

MRT letter: Expression of ATP sensor protein in Caenorhabditis elegans

Adenosine 5'-triphosphate (ATP) is the major energy currency and is involved in many biological processes. The ATP-monitoring system for cells in animals can be helpful to study the relationship between energy metabolism and biological processes. The fluorescent ATP biosensor ATeam (ATP indicator based on Epsilon subunit for Analytical Measurements), which has been reported to monitor ATP levels in cultured cells on the basis of fluorescence resonance energy transfer (FRET), was introduced into nematodes by microinjection and UV-irradiation method. To confirm whether ATeam functions as an ATP sensor in nematode cells, the authors measured FRET of ATeam in cells of transgenic nematode. The ATeam was expressed in target cells in nematode. In vulva cells, ATP levels in the cytosol were higher than those in mitochondria. ATeam also sensed ATP level change in cultured cells from the transgenic nematode. These experiments indicated that ATeam is available for detection of changes in ATP levels in nematode cells.     

Highly efficient deep‐blue fluorescent dopant for achieving low‐power OLED display satisfying BT.2020 chromaticity

In this work, novel blue‐fluorescent dopants with a heteroaromatic ring skeleton instead of the conventional pyrene skeleton were investigated. Bottom‐emission organic light‐emitting diodes (OLEDs) fabricated using the novel blue‐fluorescent dopants in light‐emitting layers achieved better deep‐blue chromaticity than OLEDs based on a conventional pyrene‐based dopant, while maintaining both high external quantum efficiency (EQE) and comparable reliability. The attainment of deep‐blue chromaticity without losing high EQE was ascribed to the improvement of the efficiency of energy transfer from the host to the dopant. Furthermore, it was estimated that using this novel dopant in a top‐emission OLED panel that satisfies BT.2020 chromaticity enables the power consumption of the whole panel to be 24% lower than that of the panel with a conventional dopant.     

Nano-crystalline LiNi0.5Mn1.5O4 synthesized by emulsion drying method

LiNi_0.5Mn_1.5O₄ spinel has been prepared by an emulsion drying method which can intermix cations very homogeneously at the atomic scale. When the emulsion-dried precursor was fired at 750 °C for 24 h, the observed particle of the LiNi_0.5Mn_1.5O₄ was nano-crystallite, being about 50 nm in diameter. The Rietveld refinement result clearly exhibited that the cubic spinel phase was successfully formed without any secondary phases, indicating that Li and transition metal cations occupied the 8a and 16d sites of the Fd3m structure, respectively. Li deintercalation from the spinel framework brings about a shift in the XRD peak toward higher angles and a peak splitting in the composition range δ=0-0.2 in Li_δNi_0.5Mn_1.5O₄, implying that the host structure is progressively oxidized from Ni²⁺ to Ni⁴⁺ and accompanied by a two phase reaction. The sample calcined at 750 °C for 24 h showed the best cyclability upon cycling due probably to better crystallinity and a smaller particle size. We suggest that this material can be used as a 4.5 V cathode material for Li-ion battery.     

Influence of manganese(II), cobalt(II), and nickel(II) additives in electrolyte on performance of graphite anode for lithium-ion batteries

Manganese dissolution into an electrolyte from the spinel LiMn₂O₄ in the lithium-ion cell has been recently investigated. In order to study the influence of the dissolved manganese species on the lithium intercalation/deintercalation into a natural graphite electrode, the electrochemical behavior of graphite was investigated in 1 mol dm⁻³ LiClO₄ electrolyte solution containing a small amount of Mn(II) by the addition of manganese(II) perchlorate. During the charging process, Mn(II) ions were firstly electroreduced on the electrode around 1.0 V versus Li/Li⁺ followed by irreversible decomposition of the electrolyte and lithium intercalation into the graphite. By microscopic observation of the graphite surface, manganese deposition was confirmed after the charge/discharge test. Due to the manganese deposition, the reversible capacity of the graphite electrode was drastically decreased. Furthermore, the cyclability of the anode was degraded with the amount of the manganese additive increasing. We compared these results with those of the cobalt(II) and nickel(II) additives by dissolving the corresponding perchlorates. Furthermore, we discussed the influence in practical cells based on the consideration of electrochemistry of the deposited metals.     

Metal cluster complex primary ion beam source for secondary ion mass spectrometry (SIMS)

To develop a solution-type ion beam source utilizing a wide variety of metal cluster complexes that are stable only in organic solvents, we have investigated an electrospray method for transferring ions from solutions to gas phase. As initial experiments, we have studied electrospray characteristics of ethanol solutions containing a room-temperature molten salt (i.e., an ionic liquid) and acetic acid as alternatives to solutions of metal cluster complexes. In electrospray experiments, we used a stainless-steel capillary with an inner diameter of 30 μm. Experimental results showed that electrosprayed currents increased with applied voltage in both positive-ion and negative-ion modes. In addition to positive currents, stable negative currents were also confirmed to be produced. Current exceeding 250 nA was produced at 2 kV with a flow rate of 2 μL/min at a concentration of 1 × 10⁻³ mol/L. It was confirmed that several nA out of electrosprayed currents were delivered through an orifice (120 μm internal diameter) into a vacuum chamber. Experimental results indicate that the electrospray method seems to be applicable to an ion beam source for utilizing massive metal cluster complexes in solutions.     

Suppressed Hepatic Production of Indoxyl Sulfate Attenuates Cisplatin-Induced Acute Kidney Injury in Sulfotransferase 1a1-Deficient Mice

Endogenous factors involved in the progression of cisplatin nephropathy remain undetermined. Here, we demonstrate the toxico-pathological roles of indoxyl sulfate (IS), a sulfate-conjugated uremic toxin, and sulfotransferase 1A1 (SULT1A1), an enzyme involved in its synthesis, in cisplatin-induced acute kidney injury using Sult1a1-deficient (Sult1a1^-/- KO) mice. With cisplatin administration, severe kidney dysfunction, tissue damage, and apoptosis were attenuated in Sult1a1^-/- (KO) mice. Aryl hydrocarbon receptor (AhR) expression was increased by treatment with cisplatin in mouse kidney tissue. Moreover, the downregulation of antioxidant stress enzymes in wild-type (WT) mice was not observed in Sult1a1^-/- (KO) mice. To investigate the effect of IS on the reactive oxygen species (ROS) levels, HK-2 cells were treated with cisplatin and IS. The ROS levels were significantly increased compared to cisplatin or IS treatment alone. IS-induced increases in ROS were reversed by downregulation of AhR, xanthine oxidase (XO), and NADPH oxidase 4 (NOX4). These findings suggest that SULT1A1 plays toxico-pathological roles in the progression of cisplatin-induced acute kidney injury, while the IS/AhR/ROS axis brings about oxidative stress.