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排序方式: 共有133条查询结果,搜索用时 15 毫秒
81.
Sanjeev K. Sharma Hideki Zushi Yuki Hisano Yuta Higashizono Sigeru Morita Naoaki Yoshida Makoto Hasegawa Kazuo Nakamura Kohnosuke N. Sato Hisatoshi Nakashima Yousuke Nakashima Yuji Hatano Yukio Nakamura Takashi Maekawa Yuichi Takase QUEST Group 《Fusion Engineering and Design》2010,85(6):950-955
Particle retention and recycling in plasma fusion devices are generally associated with the diffusion of atomic hydrogen into the materials. The resulted permeation of atomic hydrogen is known as plasma driven permeation (PDP). This permeation may also be significant, even in the walls, which are not directly exposed to the plasma. Under similar conditions, the permeation flux (Γperm) of hydrogen through a 30 μm thick Ni membrane heated at 412-575 K has been measured in the spherical tokamak QUEST. Γperm is being measured during the scans of different operating parameters like RF power (PRF), chamber pressure (Pchamber), discharge widths (τdis) and vertical magnetic field (BZ). Simultaneously edge plasma density and spectral intensities of atomic (Balmer) lines and molecular (Fulcher) bands have been compared with the permeation measurements. A linear relationship has been established between the time integrated Γperm i.e. permeation fluence (Qperm) and the time integrated Hα intensity i.e. Hα fluence (Qα). Qperm also shows a strong relationship with the edge plasma density and various spectral fluences. The obtained results are discussed for exploring the applicability of the permeation probes in measuring the atomic flux near the first walls. 相似文献
82.
We investigated the efficacy of a loop-mediated isothermal amplification (LAMP) assay for rapid screening of seafood samples naturally contaminated with Vibrio parahaemolyticus. A total of 171 seafood samples enriched in alkaline peptone water (APW) were assessed by LAMP assay and conventional culture methods, which consist of a combination of APW enrichment culture and plating onto CHROMagar Vibrio and TCBS agars. Compared with V. parahaemolyticus isolation using the conventional culture test, LAMP results showed 100% (30/30) and 90.8% (128/141) sensitivity and specificity, respectively. The conventional culture test required more than 3 days to isolate and identify V. parahaemolyticus in the APW enrichment culture. In contrast, the LAMP assay was markedly faster, requiring less than 60 min from the beginning of DNA extraction to final detection of V. parahaemolyticus. In total, the LAMP assay required 17-19 h from the beginning of enrichment culture to final determination. This is the first report of the LAMP assay for rapid screening of seafood samples naturally contaminated by V. parahaemolyticus. 相似文献
83.
Yuji Hatano Jie Shi Naoaki Yoshida Naoki Futagami Yasuhisa Oya Hirofumi Nakamura 《Fusion Engineering and Design》2012,87(7-8):1091-1094
To examine the resolution of isotope analysis of hydrogen with glow-discharge optical emission spectroscopy (GDOES), depth profiles of hydrogen and deuterium in a H-containing Ta/D-containing Ti/Ni layered structure were measured. The depth profiles of deuterium could be measured with sufficient resolution in the presence of relatively large amounts of hydrogen and vice versa. In addition, measurements of depth profiles of He implanted in W at room temperature were also performed with Ne plasma. The intensity of the He emissions was sufficiently high at a fluence of 1020 He m?2 or higher. The depth profiles of He measured in this manner were in good agreement with the results of cross-sectional observations using a transmission electron microscope. Therefore, it was concluded that GDOES with Ne plasma is a promising technique for the depth profile analysis of plasma-facing materials and deposited layers formed on them. 相似文献
84.
Molecular cloning of the gamma-glutamylcysteine synthetase gene of Saccharomyces cerevisiae. 总被引:5,自引:0,他引:5
The 4.4 kb SphI DNA fragment (GSH1) that complements the gamma-glutamylcysteine synthetase-deficient mutation (gsh1) of Saccharomyces cerevisiae YH1 was cloned into vector plasmid YEp24. Gene disruption of the cloned fragment confirmed that this segment was the same gene as gsh1. Mutant strain YH1 with this plasmid not only restored gamma-glutamylcysteine synthetase (GSH-I) activity but the glutathione content and the growth rate. DNA sequence analysis of the SphI fragment showed that the GSH1 structural gene contained 2034 bp and predicted a polypeptide of 678 amino acids. The deduced amino acid sequence had about a 45% homology to that of rat kidney GSH-I, but a very low homology (about 26%) to that of Escherichia coli GSH-I. Northern analysis showed that GSH1 had been transcribed into an approximately 2.7 kb mRNA fragment. Southern analysis showed that GSH1 mapped at chromosome X. 相似文献
85.
All-solid-state thin-filmed lithium-ion rechargeable batteries composed of amorphous Nb2O5 negative electrode with the thickness of 50–300 nm and amorphous Li2Mn2O4 positive electrode with a constant thickness of 200 nm, and amorphous Li3PO4−xNx electrolyte (100 nm thickness), have been fabricated on glass substrates with a 50 mm × 50 mm size by a sputtering method, and their electrochemical characteristics were investigated. The charge–discharge capacity based on the volume of positive electrode increased with increasing thickness of negative electrode, reaching about 600 mAh cm−3 for the battery with the negative electrode thickness of 200 nm. But the capacity based on the volume of both the positive and negative electrodes was the maximum value of about 310 mAh cm−3 for the battery with the negative electrode thickness of 100 nm. The shape of charge–discharge curve consisted of a two-step for the batteries with the negative electrode thickness more than 200 nm, but that with the thickness of 100 nm was a smooth S-shape curve during 500 cycles. 相似文献
86.
Suzuki M Iwashima A Tsutsui N Ohira T Kogure T Nagasawa H 《Chembiochem : a European journal of chemical biology》2011,12(16):2478-2487
The nacreous layer of molluscan shells consists of a highly organised, layered structure comprising calcium carbonate aragonite crystals, each surrounded by an organic matrix. In the Japanese pearl oyster Pinctada fucata, the Pif protein from the nacreous layer functions in aragonite binding, and plays a key role in nacre formation. Here, we investigated whether the blue mussel Mytilus galloprovincialis also has a protein with similar functions in the nacreous layer. By using a calcium carbonate-binding assay, we identified the novel protein blue mussel shell protein (BMSP) 100 that can bind calcium carbonate crystals of both aragonite and calcite. When the entire sequence of a cDNA encoding BMSP 100 was determined, it was found that BMSP is a preproprotein consisting of a signal peptide and two proteins, BMSP 120 and BMSP 100. BMSP 120 contains four von Willebrand factor A (VWA) domains and one chitin-binding domain, thus suggesting that it has a role in maintaining structure within the matrix. Immunohistochemical analysis revealed that BMSP 100 is present throughout the nacreous layer with dense localisation in the myostracum. Posttranslational modification analysis indicated that BMSP 100 is phosphorylated and glycosylated. These results suggest that there is a common molecular mechanism between P. fucata and M. galloprovincialis that underlies the nacreous layer formation. 相似文献
87.
Morihara N Nishihama T Ushijima M Ide N Takeda H Hayama M 《Molecular nutrition & food research》2007,51(11):1329-1334
More than three thousand publications in the past have confirmed the efficacy of garlic for the prevention and treatment of a variety of diseases, acknowledging and validating its traditional uses. Garlic is also used for the treatment of fatigue, although the mechanism involved remain unclear. The anti-fatigue function of garlic may be closely related to its many favorable biological and pharmacological effects. In animal studies, garlic has been shown to promote exercise endurance. Differences in the methods of processing garlic result in differences in the intensity of its anti-fatigue effect, and the most favorable form of processing has been shown to be extraction of raw garlic followed by its natural aging for a long period in a water-ethanol mixture. In human studies, it has been confirmed that garlic produces symptomatic improvement in persons with physical fatigue, systemic fatigue due to cold, or lassitude of indefinite cause, suggesting that garlic can resolve fatigue through a variety of actions. Recently, primarily in Japan, attempts have been made to measure the intensity of fatigue objectively and quantitatively using biomarkers. Currently available data strongly suggest that garlic may be a promising anti-fatigue agent, and that further studies to elucidate its application are warranted. 相似文献
88.
Crystallization and preliminary X-ray analysis of salicylate hydroxylase from Pseudomonas putida S-1
T Yabuuchi K Suzuki T Sato K Ohnishi E Itagaki Y Morimoto 《Canadian Metallurgical Quarterly》1996,119(5):829-831
Apo-salicylate hydroxylase from Pseudomonas putida S-1 has been crystallized by the dialysis method, using ammonium sulfate as the precipitant. The crystals belong to hexagonal space group P6(2) or P6(4) with unit cell dimensions of a = b = 142.8 A and c = 63.8 A, and diffract X-rays at higher than 3.5 A resolution. A heavy-atom derivative has been prepared by soaking a crystal in an ammonium sulfate solution containing p-chloromercuriphenylsulfonate. 相似文献
89.
Large-scale high-energy batteries with electrode materials made from the Earth-abundant elements are needed to achieve sustainable energy development. On the basis of material abundance, rechargeable sodium batteries with iron- and manganese-based positive electrode materials are the ideal candidates for large-scale batteries. In this review, iron- and manganese-based electrode materials, oxides, phosphates, fluorides, etc, as positive electrodes for rechargeable sodium batteries are reviewed. Iron and manganese compounds with sodium ions provide high structural flexibility. Two layered polymorphs, O3- and P2-type layered structures, show different electrode performance in Na cells related to the different phase transition and sodium migration processes on sodium extraction/insertion. Similar to layered oxides, iron/manganese phosphates and pyrophosphates also provide the different framework structures, which are used as sodium insertion host materials. Electrode performance and reaction mechanisms of the iron- and manganese-based electrode materials in Na cells are described and the similarities and differences with lithium counterparts are also discussed. Together with these results, the possibility of the high-energy battery system with electrode materials made from only Earth-abundant elements is reviewed. 相似文献
90.