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71.
Microstructural observations and creep measurements at elevated temperatures were carried out on nickel-base cast superalloys strengthened by 0.65 atomic fraction gamma-prime (y'J phase with varied concentrations of Ta, Wand Mo and with elements commonly used in practice. The variations in the amounts of the refractory elements considerably altered the morphologies and the quantities of carbides as well as γ and γ’ eutectics. The steady-state creep rate for these alloys was related to lattice mismatch between the γ and γ’ phases as well as to the aluminium concentration in the γ’ phase. The effect of the elements on creep rupture life was in the order W>Ta>Mo. 相似文献
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74.
Hiroshi Isakari Kazuki Niino Hitoshi Yoshikawa Naoshi Nishimura 《International journal for numerical methods in engineering》2012,90(4):484-505
Preconditioning methods based on Calderon's formulae for the periodic fast multipole method for elastodynamics in 3D are investigated. Three different types of formulations are proposed. The first type is a preconditioning just by appropriately ordering the coefficient matrix without multiplying preconditioners. The other two types utilise preconditioners constructed using matrices needed in the main fast multipole method algorithms. We make several numerical experiments with proposed preconditioners to confirm the efficiency of these proposed methods. We also conclude that the preconditioning of the first type is faster with respect to the computational time than other preconditioning methods discussed in this article. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
75.
A Multiple‐Labeling Strategy for Nonribosomal Peptide Synthetases Using Active‐Site‐Directed Proteomic Probes for Adenylation Domains 下载免费PDF全文
Dr. Fumihiro Ishikawa Dr. Takehiro Suzuki Dr. Naoshi Dohmae Prof. Hideaki Kakeya 《Chembiochem : a European journal of chemical biology》2015,16(18):2590-2594
Genetic approaches have greatly contributed to our understanding of nonribosomal peptide biosynthetic machinery; however, proteomic investigations are limited. Here, we developed a highly sensitive detection strategy for multidomain nonribosomal peptide synthetases (NRPSs) by using a multiple‐labeling technique with active‐site‐directed probes for adenylation domains. When applied to gramicidin S‐producing and ‐nonproducing strains of Aneurinibacillus migulanus (DSM 5759 and DSM 2895, respectively), the multiple technique sensitively detected an active multidomain NRPS (GrsB) in lysates obtained from the organisms. This functional proteomics method revealed an unknown inactive precursor (or other inactive form) of GrsB in the nonproducing strain. This method provides a new option for the direct detection, functional analysis, and high‐resolution identification of low‐abundance active NRPS enzymes in native proteomic environments. 相似文献
76.
Guo-Dong Zhan Yuichi Ikuhara Mamoru Mitomo Rong-Jun Xie Taketo Sakuma Amiya K. Mukherjee 《Journal of the American Ceramic Society》2002,85(2):430-436
The microstructures of fine-grained β-SiC materials with α-SiC seeds annealed either with or without uniaxial pressure at 1900°C for 4 h in an argon atmosphere were investigated using analytical electron microscopy and high-resolution electron microscopy (HREM). An applied annealing pressure can greatly retard phase transformation and grain growth. The material annealed with pressure consisted of fine grains with β-SiC as a major phase. In contrast, the microstructure in the material annealed without pressure consisted of elongated grains with half α-SiC. Energy-dispersive X-ray analysis showed no differences in the amount of segregation of aluminum and oxygen atoms at grain boundaries, but did show a significant difference in the segregation of yttrium atoms at grain boundaries along SiC grains for the two materials. The increased segregation of yttrium ions at grain boundaries caused by the applied pressure might be the reason for the retarded phase transformation and grain growth. HREM showed a thin secondary phase of 1 nm at the grain boundary interface for both materials. The development of a composite grain consisting of a mixture of β/α polytypes during annealing was a feature common to both materials. The possible mechanisms for grain growth and phase transformation are discussed. 相似文献
77.
Hydrothermal Synthesis and Low-Temperature Sintering of Zinc Gallate Spinel Fine Particles 总被引:1,自引:0,他引:1
Nanosized powders of single-phase zinc gallate (ZnGa2 O4 ) spinel were hydrothermally synthesized from solutions in the presence of NaOH over the pH range of 1.9 to 7.0 and from solutions above pH 7.0, i.e., the very basic medium (pH of 13.85), by removing the residual ZnO phase by washing with aqueous H2 SO4 from the precipitate mixtures of zinc gallate spinel particles and ZnO. A very wide compositional range (Zn/2Ga = 0.705–1.157) of zinc gallate spinel solid solutions could be hydrothermally synthesized in the form of nanosized particles from acid and very basic mediums (pH of 2.4–13.85) in the presence of NaOH. These hydrothermally synthesized spinel powders showed good sinterability and almost full densification at 1100°C for 1 h. Dense sintered bodies consisting of single-phase zinc gallate spinel were fabricated at 1100°C using zinc gallate spinel powders having almost stoichiometric composition formed from the solution at pH 9.95 in the presence of aqueous ammonia. 相似文献
78.
B. Carol Johnson F. Sakuma J. J. Butler S. F. Biggar J. W. Cooper J. Ishida K. Suzuki 《Journal of research of the National Institute of Standards and Technology》1997,102(6):627-646
As a part of the pre-flight calibration and validation activities for the Ocean Color and Temperature Scanner (OCTS) and the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) ocean color satellite instruments, a radiometric measurement comparison was held in February 1995 at the NEC Corporation in Yokohama, Japan. Researchers from the National Institute of Standards and Technology (NIST), the National Aeronautics and Space Administration/Goddard Space Flight Center (NASA/GSFC), the University of Arizona Optical Sciences Center (UA), and the National Research Laboratory of Metrology (NRLM) in Tsukuba, Japan used their portable radiometers to measure the spectral radiance of the OCTS visible and near-infrared integrating sphere at four radiance levels. These four levels corresponded to the configuration of the OCTS integrating sphere when the calibration coefficients for five of the eight spectral channels, or bands, of the OCTS instrument were determined. The measurements of the four radiometers differed by −2.7 % to 3.9 % when compared to the NEC calibration of the sphere and the overall agreement was within the combined measurement uncertainties. A comparison of the measurements from the participating radiometers also resulted in agreement within the combined measurement uncertainties. These results are encouraging and demonstrate the utility of comparisons using laboratory calibration integrating sphere sources. Other comparisons will focus on instruments that are scheduled for spacecraft in the NASA study of climate change, the Earth Observing System (EOS). 相似文献
79.
S Suzuki T Sakuma K Koike S Ono T Tanita S Fujimura 《Canadian Metallurgical Quarterly》1996,34(5):506-510
To determine the effect of time in culture on epithelial cell function, we evaluated the modulation of Na(+)-K(+)-ATPase activity in rat alveolar type II cells in culture. Ouabain sensitivity testing revealed that the alpha-1 predominance in the enzyme's isoforms was maintained over the 120 hours in culture. Basal Na(+)-K(+)-ATPase activity in the whole cell homogenate did not differ significantly between cells cultured for 48 hours and those cultured for 120 hours. Terbutaline (10 mM) did not activate Na(+)-K(+)-ATPase in the cells cultured for 48 hours, but, it significantly increased the activity of this enzyme in the cells cultured for 120 hours cells cultured for 48 hours, produced intracellular cyclic AMP after exposure to 10 mM of terbutaline. These results indicate that the coupling between Na(+)-K(+)-ATPase and the beta-adrenergic pathway in alveolar type II cells can be influenced by the time in cell culture. 相似文献
80.
Fukawa M. Kawai T. Okano Y. Sakuma S. Asai S. Kanaoka M. Yamanouchi H. 《Power Delivery, IEEE Transactions on》1996,11(2):627-634
500-kV XLPE-insulated cable with an insulation thickness of 27 mm has been developed for long-distance transmission lines. Basic studies on 500-kV XLPE cable have shown that contaminants in the insulation may be the factor determining electrical performance. This hypothesis is justified by the good correlation obtained between statistical estimations of the size of the largest contaminant in the insulation and the electrical characteristics of full-sized cables. Voltage-withstand and long-term tests have confirmed the design values for minimum breakdown stress, for AC and impulse voltage, and for the degradation coefficients 相似文献