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71.
72.
M Maruyama T Kudo H Kuwahara S Takamatsu N Sugano M Ebuchi 《Canadian Metallurgical Quarterly》1997,24(13):2011-2014
A 28-year-old female underwent sigmoidectomy for sigmoid colon cancer with peritoneal seeding. One month later, a solitary metastasis was found in S3 of the liver. After CDDP/5-FU intravenous chemotherapy, another metastasis appeared in S7. Intravenous administration showed PD. But the metastatic tumors shrank and became inobservable by CT after the 1st round of CDDP/5-FU intraperitoneal chemotherapy, and S7 tumor could not be identified after the 2nd round. Many previous reports demonstrated the concentration of cytotoxic drug in intraperitoneal administration was much higher than in intravenous administration. Theoretically, intraperitoneal chemotherapy is superior to intravenous chemotherapy for the prevention and treatment of liver metastases. This case demonstrated this hypothesis was right. We think adjuvant intraperitoneal chemotherapy should be re-considered for the prevention of the liver metastases of gastrointestinal cancers. 相似文献
73.
The cDNAs and genes for two isozymes of cytochrome P450nor of the fungus Cylindrocarpon tonkinense, P450nor1 and P450nor2, were cloned and sequenced. Their deduced amino acid sequences respectively showed 83 and 70% identity to that of P450nor of Fusarium oxysporum, and 69% identity to each other. The genes for P450nor1 and P450nor2 were termed, respectively, CYP 55A2 and CYP 55A3. The cDNA for P450nor1 contained a targeting-like presequence upstream the N-terminus of mature protein whereas that for P450nor2 did not, suggesting their different intracellular localisations. We also succeeded in expressing these P450nor isoforms in the host-vector system of the yeast Saccharomyces cerevisiae. We purified one of the recombinant proteins, P450nor of F oxysporum. Little difference could be observed between the native and recombinant proteins in catalytic and spectroscopic properties. We constructed chimeric proteins of P450nor of F oxysporum and P450nor2 which are different in their specificity against the electron donors: reduced pyridine nucleotides. The specificity of chimeric proteins against NADH/NADPH showed that the specificity is determined by the N-terminal half of protein. We found a consensus amino acid sequence between three isoforms of P450nor, A-X-G-X-X-A, similar to the NAD-binding motif G-X-G-X-X-G/A in the region that corresponds to the B'-helix in P450cam. 相似文献
74.
75.
Isono N Tochihara T Kusnadi Y Win TT Watanabe K Obae K Ito H Matsui H 《Journal of Bioscience and Bioengineering》2004,97(4):244-249
We have cloned the gene encoding a beta-fructofuranosidase from Arthrobacter globiformis IFO 3062, and subsequently, the gene was heterologously expressed in Escherichia coli. This beta-fructofuranosidase gene encodes a protein of 548 amino acid residues with a calculated molecular mass of 60,519 Da. We have examined the roles of three residues of A. globiformis IFO 3062 beta-fructofuranosidase by site-directed mutagenesis, and found that aspartic acid 130 and glutamic acid 392, which are two of the apparent catalytic residues, are essential for hydrolase activity. This study provides the first experimental evidence showing that these two amino acid residues of beta-fructofuranosidase play a critical role in hydrolyzing sucrose. 相似文献
76.
General reconstruction theory for multislice X-ray computed tomography with a gantry tilt 总被引:3,自引:0,他引:3
This paper discusses image reconstruction with a tilted gantry in multislice computed tomography (CT) with helical (spiral) data acquisition. The reconstruction problem with gantry tilt is shown to be transformable into the problem of reconstructing a virtual object from multislice CT data with no gantry tilt, for which various algorithms exist in the literature. The virtual object is related to the real object by a simple affine transformation that transforms the tilted helical trajectory of the X-ray source into a nontilted helix, and the real object can be computed from the virtual object using one-dimensional interpolation. However, the interpolation may be skipped since the reconstruction of the virtual object on a Cartesian grid provides directly nondistorted images of the real object on slices parallel to the tilted plane of the gantry. The theory is first presented without any specification of the detector geometry, then applied to the curved detector geometry of third-generation CT scanners with the use of Katsevich's formula for example. Results from computer-simulated data of the FORBILD thorax phantom are given in support of the theory. 相似文献
77.
Matsumoto T Harano H Ito Y Uritani A Emi K Kudo K 《Radiation protection dosimetry》2004,110(1-4):223-226
A new fast neutron spectrometer has been developed. The spectrometer is composed of a silicon surface barrier detector and three position-sensitive proportional counters with methane gas working as counting gas and a radiator. A collimated incident neutron interacts with a hydrogen atom in the methane gas to generate a recoil proton. The position information on the path of the recoil proton obtained from the three position-sensitive proportional counters gives the recoil angle. In the meanwhile, the energy of the recoil protons is measured with the three proportional counters and the silicon surface barrier detector. The characteristics of the spectrometer were evaluated with a monoenergetic neutron beam. The best energy resolution was 1.8% for 5.0 MeV neutrons. 相似文献
78.
Ito Y Katano G Harano H Matsumoto T Uritani A Kudo K Kobayashi K Yoshimoto T Sakurai Y Kobayashi T Mori C 《Radiation protection dosimetry》2004,110(1-4):619-622
We have developed a tiny neutron probe detector as a monitor of a thermal neutron flux for boron neutron capture therapy. The detector consists of an optical fibre and a small neutron probe. We have used a film-like ZnS(Ag) scintillator and a 6LiF neutron converter for the neutron probe. In order to improve the gamma-neutron discrimination ability, vacuum evaporation of 6LiF onto the ZnS(Ag) film has been done. In order to improve the neutron detection efficiency, we made use of a wavelength-shifting fibre as the probe material. The characteristics of the above two types of fibre probe detector have been evaluated experimentally. 相似文献
79.
Tanimura Y Yoshizawa M Saegusa J Fujii K Shimizu S Yoshida M Shibata Y Uritani A Kudo K 《Radiation protection dosimetry》2004,110(1-4):85-89
Monoenergetic neutron calibration fields of 144, 565 keV and 5.0 MeV have been developed at the Facility of Radiation Standards of JAERI using a 4 MV Pelletron accelerator. The 7Li(p,n)7Be and 2H(d,n)3He reactions are employed for neutron production. The neutron energy was measured by the time-of-flight method with a liquid scintillation detector and calculated with the MCNP-ANT code. A long counter is employed as a neutron monitor because of the flat response. The monitor is set up where the influence of inscattered neutrons from devices and their supporting materials at a calibration point is as small as possible. The calibration coefficients from the monitor counts to the neutron fluence at a calibration point were obtained from the reference fluence measured with the transfer instrument of the primary standard laboratory (AIST), a 24.13 cm phi Bonner sphere counter. The traceability of the fields to AIST was established through the calibration. 相似文献
80.
The perovskite-type compounds (Ln, M)M′O3 (Ln: lanthanoid, M: alkaline earth, M′: transition metal) are synthesized, and the related perovskite formation region is determined. The resistivities of these compounds at 25°C decrease by several orders of magnitude when they are doped with Ca, Sr or Ba. The resistivity minima range from 10?4 to 10?1 Ωcm, depending on the composition. The oxygen ion diffusion constants are evaluated from the electrode potentials in a KOH solution. The diffusion constant for Nd0.8Sr0.2 CoO3 is 1.4 × 10?11 cm2/s at 25°C. These compounds are examined as potential oxygen electrode materials for alkaline solution metal fuel cells, and (Nd, Sr)CoO3 provides good performance for several hundred hours. 相似文献