Genetics of colorectal and breast cancer

Colorectal and breast cancers account for a significant number of deaths due to malignant neoplasia. Laboratory medicine plays an important role in the diagnosis and management of these tumors through the application of histopathology, immunohistochemistry, and serologic identification of tumor markers. Approximately 5% to 10% of colorectal and breast cancers result from an inherited predisposition. The genes responsible for most genetically transmitted cancers have been identified, and the application of findings from molecular pathology are being evaluated. This article reviews the genetic changes that occur as a result of somatic mutation and inherited or germline mutations.     

A general procedure for deriving solutions of dual integral equations

Summary In this paper there is developed an operational procedure for deriving solutions of dual integral equations of a general type-equations (1.1) and (1.2) below. The method depends strongly on properties of the Mellin transform. To illustrate the application of the method solutions are derived of the elementary type of dual integral equations occurring in engineering applications. The method is then applied to the solution of dual integral equations of Titchmarsh type and to those involvingY-andK-transforms.Research supported by a National Research Council of Canada grant.     

Determination of the leaching rate of Cs from the immobilized low-level radioactive sources in the cement and cement mixed with dolomite grains and with natural pozzolan powder

The leaching rate of ¹³⁴Cs from the immobilized low-level radioactive source in the Portland cement was found to be 4.481 × 10⁻⁴ g/cm² per day. Mixing this cement with dolomite grains and natural pozzolan powder increased and reduced significantly the leaching rate to 7.373 × 10⁻⁴ g/cm² and 3.495 × 10⁻⁴ g/cm² per day for 1 wt% mixing, and to 12.340 × 10⁻⁴ g/cm² and 3.215 × 10⁻⁵ g/cm² per day for 3 wt% mixing, respectively. This increase and reduction of the leaching rate is due to dedolomitization reactions between dolomite grains and cement alkalis, and pozzolanic reaction between pozzolan powder and calcium hydroxide, respectively. It was also found that the application of a latex paint reduced these leaching rates by about 8.8 and 8.2% for cement mixed with dolomite and with pozzolan, respectively. The obtained results help to improve the concrete properties used for the storage and disposal of the low-level radioactive wastes.     

In vitro evaluation of biopolymer networks based on crosslinked cellulose with various diamines

In this study, we investigated some hydrogels based on natural, biodegradable, and biocompatible polysaccharides and cellulose, as sustained release carriers and evaluated their controlled drug release. The crosslinking of bromoacetylated cellulose with various aliphatic and aromatic diamines, such as ethylene diamine, hexamethylene diamine, and paraphenylene diamine, were carried out in N,N‐dimethylformamide in the presence of triethylamine as a base. On the other hand, the synthesized hydrogels were characterized with Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, and CHN elemental analysis. Also, the equilibrium swelling behavior of the hydrogels in water was calculated, and this indicated their sustained expansion. The loading of ceftizoxime antibiotic was carried out on the prepared hydrogels, and the in vitro and drug‐release behaviors were investigated in three different media (HCl solution at pH 3 and bicarbonate buffer solutions at pH 6 and 8). Finally, the release profiles of ceftizoxime from the hydrogels and their loading capacities were determined by ultraviolet–visible absorption measurements at λ_max 295 nm. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 42568.     

Analysis of Effective Environmental Flow Release Strategies for Lake Urmia Restoration

Saline lakes have diminished considerably due to large-scale irrigation projects throughout the world. Environmental flow (EF) release from upstream reservoirs could help conserve and restore these lakes. However, experiences from regions lacking environmental legislation or with insufficient water resources management show that, despite EF allocation, farmers tend to use all available water for agriculture. In this study, we employed a new method for designing environmental flow release strategies to restore desiccated terminal lakes in arid and semi-arid regions with intensive cultivation within the catchment. The novelty of the method is that it takes into account farmers’ water use behavior and the natural flow regime in upstream systems to design an optimum monthly EF release strategy for reservoirs. We applied the method to the water resource system of Lake Urmia, once the largest saline lake in the Middle East and now one of the most endangered saline lakes in the world. The analysis showed that the EF released is exploited by lowland farmers before reaching Lake Urmia and that inflow to the lake from some rivers has decreased by up to 80%. We propose a new EF release strategy that requires a considerable change in practice whereby water is released in the shortest possible time (according to reservoir outlet capacity) during the period of lowest irrigation demand in winter. Restoring the lake to minimum ecological level would require 2.4–3.4 km³ EF allocation by different methods of release based on the recent condition (2002–2011) of the lake.     

Applications of network coding to improve TCP performance over wireless mesh networks: a survey

There is growing interest in the use of wireless mesh network (WMN) as a last‐mile option for Internet access. Despite the many benefits of WMNs, the performance of Internet access may not be ideal. One of the main issues is the interaction of transmission control protocol (TCP) with the underlying network. The poor performance of TCP over multi‐hop networks is well‐documented, and extensive research exists, which addresses TCPs foible and enhance TCP performance for multi‐hop environments. This paper provides a thorough survey of TCP performance issues over WMNs and the available solutions to address these issues. Among the existing methods, we focused on network coding (NC) and the ways that TCP interacts with network coded systems. NC is a technique that encodes the received packets in each node before forwarding them towards the destination. The use of NC in the transport layer to address performance issues raised by wireless access is a recent research topic. This paper presents a detailed study of TCP interaction with NC. Some open research areas in this field are suggested. Copyright © 2012 John Wiley & Sons, Ltd.     

Controlling Mechanical Properties of Cell‐Laden Hydrogels by Covalent Incorporation of Graphene Oxide

Graphene‐based materials are useful reinforcing agents to modify the mechanical properties of hydrogels. Here, an approach is presented to covalently incorporate graphene oxide (GO) into hydrogels via radical copolymerization to enhance the dispersion and conjugation of GO sheets within the hydrogels. GO is chemically modified to present surface‐grafted methacrylate groups (MeGO). In comparison to GO, higher concentrations of MeGO can be stably dispersed in a pre‐gel solution containing methacrylated gelatin (GelMA) without aggregation or significant increase in viscosity. In addition, the resulting MeGO‐GelMA hydrogels demonstrate a significant increase in fracture strength with increasing MeGO concentration. Interestingly, the rigidity of the hydrogels is not significantly affected by the covalently incorporated GO. Therefore, this approach can be used to enhance the structural integrity and resistance to fracture of the hydrogels without inadvertently affecting their rigidity, which is known to affect the behavior of encapsulated cells. The biocompatibility of MeGO‐GelMA hydrogels is confirmed by measuring the viability and proliferation of the encapsulated fibroblasts. Overall, this study highlights the advantage of covalently incorporating GO into a hydrogel system, and improves the quality of cell‐laden hydrogels.     

Local Insulin-Derived Amyloidosis Model Confronted with Silymarin: Histological Insights and Gene Expression of MMP,TNF-α, and IL-6

Amyloidosis is a heterogeneous group of protein deposition diseases associated with the presence of amyloid fibrils in tissues. Analogs of insulin that are used for treating diabetic patients (including regular insulin) can form amyloid fibrils, both in vitro and in vivo as reported in patients. The main purpose of this study was the induction of localized insulin-generated amyloidosis and the observation of silymarin effects on this process. In order to obtain amyloid structures, regular insulin was incubated at 37 °C for 24 h. Congo red absorbance and transmission electron microscopy images validated the formation of amyloid fibrils. Those fibrils were then injected subcutaneously into rats once per day for 6, 12 or 18 consecutive days in the presence or absence of silymarin, and caused development of firm waxy masses. These masses were excised and stained with Hematoxylin and Eosin, Congo red and Thioflavin S. Histological examination showed adipose cells and connective tissue in which amyloid deposition was visible. Amyloids decreased in the presence of silymarin, and the same effect was observed when silymarin was added to normal insulin and injected subsequently. Furthermore, plasma concentrations of MMP2, TNF-α, and IL-6 inflammatory factors were measured, and their gene expression was locally assessed in the masses by immunohistochemistry. All three factors increased in the amyloidosis state, while silymarin had an attenuating effect on their plasma levels and gene expression. In conclusion, we believe that silymarin could be effective in counteracting insulin-generated local amyloidosis.     

Assessment and Rehabilitation of Embankment Dams

A series of observations, studies, and analyses to be made in the field and in the office are presented to gain a proper understanding of how an embankment dam fits into its geologic setting and how it interacts with the presence of the reservoir it impounds. It is intended to provide an introduction to the engineering challenges of assessment and rehabilitation of embankments, with particular reference to a Croton Dam embankment.