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81.
Using isothermal heating, inactivation of lactoperoxidase (LPO) in goat, sheep and cow milk was studied in the temperature range of 70–77 °C. Kinetic and thermodynamics studies were carried out at different time–temperature combination in order to evaluate the suitability of LPO as marker for the heat-treatment of milk and dairy products from different species. The thermal inactivation of LPO followed the first-order kinetics. D- and k-values decreased and increased, respectively with increasing temperature, indicating a more rapid LPO inactivation at higher temperatures. The influence of temperature on the inactivation rate constant was quantified using the Arrhenius and thermal death time models. The corresponding z-values were 3.38 ± 0.013, 4.11 ± 0.24 and 3.58 ± 0.004 °C in goat, sheep and cow milk, respectively. Activation energy values varied between milk species with 678.96 ± 21.43 kJ mol−1 in goat milk, 560.87 ± 28.18 kJ mol−1 in sheep milk and 641.56 ± 13.12 kJ mol−1 in cow milk, respectively.  相似文献   
82.
The advancement of nanotechnology in the last decade has developed an abundance of novel and intriguing TiO2-based nanomaterials that are widely used in many sectors, including industry (as a food additive and colorant in cosmetics, paints, plastics, and toothpaste) and biomedicine (photoelectrochemical biosensing, implant coatings, drug delivery, and new emerging antimicrobial agents). Therefore, the increased use of engineered nanomaterials in the industry has raised serious concern about human exposure and their unexpected cytotoxic effects. Since inhalation is considered the most relevant way of absorbing nanomaterials, different cell death mechanisms induced in MRC-5 lung fibroblasts, following the exposure to functionalized TiO2 NPs, were investigated. Long-term exposure to TiO2 nanoparticles co-doped with 1% of iron and nitrogen led to the alteration of p53 protein activity and the gene expression controlled by this suppressor (NF-kB and mdm2), DNA damage, cell cycle disruptions at the G2/M and S phases, and lysosomal membrane permeabilization and the subsequent release of cathepsin B, triggering the intrinsic pathway of apoptosis in a Bax- and p53-independent manner. Our results are of major significance, contributing to the understanding of the mechanisms underlying the interaction of these nanoparticles with in vitro biological systems, and also providing useful information for the development of new photocatalytic nanoparticles that are active in the visible spectrum, but with increased biocompatibility.  相似文献   
83.
Levothyroxine (LT4) is known for its use in various conditions including hypothyroidism. LT4 interaction with serum albumin may be influenced by the presence of vitamins. For this reason, we investigated the effect of vitamin C, vitamin B12, and folic acid on the complex of Bovine Serum Albumin with LT4 (BSA-LT4). UV-Vis spectroscopy was used to monitor the influence of vitamins on the BSA-LT4 complex. Fluorescence spectroscopy revealed a static quenching mechanism of the fluorescence of BSA-LT4 complex by the vitamin C and folic acid and a combined mechanism for vitamin B12. The interaction of vitamin C and folic acid with BSA-LT4 was moderate, while the binding of vitamin B12 was much stronger, extending the storage time of LT4 in blood plasma. Synchronous fluorescence found that the vitamins were closer to the vicinity of Trp than to Tyr and the effect was more pronounced for the binding of vitamin B12. The thermal stability of the BSA-LT4 complex was more evident, but no influence on the stability of BSA-LT4 complex was obtained for vitamin C. Molecular docking studies showed that vitamin C and folic acid bound the same site of the protein, while vitamin B12 bonded to a different site.  相似文献   
84.
This article proposes a technique to improve the dependability of circuits under energetic particle irradiation by resizing transistors in the most critical paths. First, the SET vulnerability of a mapped circuit is analyzed to identify the most sensitive nodes. The sensitivity of the circuit is defined by the logical and electrical masking. Once the most critical nodes are selected, a transistor sizing algorithm is able to resize the pull-up and pull-down transistors separately. The asymmetric resizing offers interesting area and performance trade-off in comparison with gate sizing and gate duplication techniques. Results show very small area and performance penalties for circuits operating at ground level for a 130-nm technology process.  相似文献   
85.
86.
In this paper, we studied the scandium adsorption from aqueous solutions on the surface of low-temperature-activated alumina products (GDAH). The GDAH samples are industrially manufactured, coming from the Bayer production cycle of the Sierra Leone bauxite as aluminium hydroxide, and further, by drying, milling, classifying and thermally treating up to dehydroxilated alumina products at low temperature. All experiments related to hydroxide aluminium activation were conducted at temperature values of 260, 300 and 400 °C on samples having the following particle sizes: <10 µm, 20 µm, <45 µm and <150 µm, respectively. The low-temperature-activated alumina products were characterised, and the results were published in our previous papers. In this paper, we studied the scandium adsorption process on the above materials and related thermodynamic and kinetic studies.  相似文献   
87.
The present work reports the influence of the loading mode provided during training under constant stress, in bending, applied to lamellar specimens of Cu-Zn-Al shape memory alloys (SMAs). During training, the specimens were bent by a load fastened at their free end, while being martensitic at room temperature and they lifted the load by one-way effect (1WE), during heating up to austenitic field. On cooling to martensite field, the lower concave surface of bent specimens was compressed, and during heating it was elongated, being subjected to a series of tension-compression cycles, during heating-cooling, respectively. Conversely, the upper convex surface of bent specimens was elongated during cooling and compressed during heating, being subjected to compression-tension cycles. Furthermore, 2WE-trained actuators were tested by means of a hydraulic installation where, this time heating-cooling cycles were performed in oil conditions. Considering that the lower concave surface of the specimens was kept in compressed state, while the upper convex surface was kept in elongated state, the study reveals the influence of the two loading modes and environments on the width of martensite plates of the specimens trained under various numbers of cycles. In this purpose, Cu-Zn-Al specimens, trained under 100-300-500 cycles, were prepared and analyzed by atomic force microscopy (AFM) as well as optical and scanning electron microscopy (OM and SEM, respectively). The analysis also included AFM micrographs corroborated with statistical evaluations in order to reveal the effects of loading mode (tension or compression) in different environmental conditions of the specimens, on the surface profile characteristics of martensite plates, revealed by electropolishing.  相似文献   
88.
89.
In this research, a new Karl Fischer water titration–principal component analysis (KFT–PCA) concept on wheat flour samples was introduced and studied. First, the evaluation of the performance of the determination of water content of wheat flour (Triticum aestivum L. subsp. aestivum) by using the KFT method was performed. The water content of white wheat flour, determined by KFT method, was in the range of 14.9–15.2 %, while for the whole-wheat and Graham flour samples, this concentration was lower (14.7 and 14.5 %, respectively) and was close to the limit of Romanian standards. The new approach on the KFT water reaction rates for wheat flour was performed. Thus, the “surface water” reaction rates of 1.2–2.1 mM/s and the “strongly bound water” reaction rates of 0.13–0.24 mM/s were obtained for flour samples. Further, these water reaction rates together with the primary KFT parameters (e.g., water concentration and normalized titration volume) were used in a multivariate statistical approach (PCA) in order to evaluate similarities–dissimilarities between samples. Among KFT parameters, the water content, the normalized titration volume, and the surface water reaction rate were important for a good classification of the flour products.  相似文献   
90.
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