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排序方式: 共有861条查询结果,搜索用时 15 毫秒
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Y Kurata I Hisatome M Tsuboi H Uenishi G Zhang M Oyaizu R Sato S Imanishi 《Canadian Metallurgical Quarterly》1998,63(12):1023-1035
Effects of sulfhydryl oxidizing and reducing agents on permeability of the tetrodotoxin (TTX)-insensitive Na-channel were investigated in guinea-pig ventricular myocytes using the whole-cell patch-clamp technique. Mercury chloride (HgCl2) at 1-100 microM irreversibly blocked Na+ currents with no significant changes in the gating kinetics. In contrast, the hydrophilic sulfhydryl oxidizing agent, thimerosal at 50-100 microM little affected Na+ permeation through the Na-channel. The Hg2+-induced block of Na+ current could be readily reversed by 1,4-dithiothreitol (DTT), an agent that reduces disulfide bonds. These results indicate that the formation of sulfur-Hg-sulfur bridge is essential for Hg2+ block. Pretreatment with DTT prevented the Hg2+ block of Na+ current, whereas Zn2+ and Cd2+ retained their abilities to block Na+ current after DTT treatment. An application of Zn2+ or Cd2+ resulted in the restoration of Hg2+ sensitivity of the DTT-treated channel. A conformational model for the Na-channel with multiple free sulfhydryl groups and native disulfide bonds could account for our experimental data regarding the effects of sulfhydryl modifying agents on the channel permeability. We conclude that the cardiac TTX-insensitive Na-channel contains functionally important free sulfhydryl groups and disulfide bonds which are accessible from the extracellular side by an aqueous pathway. These sulfhydryls would be capable of modulating the Na-channel permeability by affecting the conformation of channel pore region. 相似文献
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Methacrylic acid (MAA) and methyl methacrylate (MMA) were grafted onto nylon 6, cellulose triacetate, cotton, viscose rayon, and polyester fibers, and the stereoregularities of the grafted polymers were determined. The graft polymerization was carried out with preirradiation techniques using γ-rays from a Co 60 source. The grafted copolymers were then separated from the homopolymers by Soxhlet extraction. The grafted (branch) polymers were isolated from the trunk polymers by acid hydrolysis and their stereoregularity was determined with a 100 MHz NMR spectrometer. The stereo-regularity of PMAA or PMMA grafted onto viscose rayon or cotton fiber was different from that of the polymers formed in ordinary radical polymerization. 相似文献
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Endothelial cells (ECs) lining the inner lumen of blood vessels are continuously subjected to hemodynamic shear stress, which is known to modify EC morphology and biological activity. This paper describes a self-contained microcirculatory EC culture system that efficiently studies such effects of shear stress on EC alignment and elongation in vitro. The culture system is composed of elastomeric microfluidic cell shearing chambers interfaced with computer-controlled movement of piezoelectric pins on a refreshable Braille display. The flow rate is varied by design of channels that allow for movement of different volumes of fluid per variable-speed pump stroke. The integrated microfluidic valving and pumping system allowed primary EC seeding and differential shearing in multiple compartments to be performed on a single chip. The microfluidic flows caused ECs to align and elongate significantly in the direction of flow according to their exposed levels of shear stress. This microfluidic system overcomes the small flow rates and the inefficiencies of previously described microfluidic and macroscopic systems respectively to conveniently perform parallel studies of EC response to shear stress. 相似文献
99.
Noriaki?koudaEmail author Nobuyuki?Matsui Haruhiko?Nishimura Ferdinand?Peper 《Neural Processing Letters》2005,22(3):277-290
The Qubit neuron model is a new non-standard computing scheme that has been found by simulations to have efficient processing
abilities. In this paper we investigate the usefulness of the model for a non linear kinetic control application of an inverted
pendulum on a cart. Simulations show that a neural network based on Qubit neurons would swing up and stabilize the pendulum,
yet it also requires a shorter range over which the cart moves as compared to a conventional neural network model. 相似文献
100.
Capillary rheometry was performed over a temperature range of 170°–200°C and a shear-rate range of 3–3000 sec?1 on an unplasticized poly(vinyl chloride) compound. The data were corrected for the effect of pressure on viscosity, for pressure loss in the barrel and at the capillary entrance, and for the non-Newtonian velocity profile. The pressure coefficient of viscosity was found to be in the same order of magnitude as those previously found with linear polyethylene and butadieneacrylonitrile copolymers. The pressure–shear-rate superposition of the flow curves is valid at least approximately, although the temperature–shear-rate superposition is inapplicable. The shape of flow curves at 180°, 190°, and 200°C are concave downward when they are expressed as log-shear-stress-log-shear-rate. Similar plots at 170° and 175°C, however, are very different; shear stress is independent of shear rate at low shear rates, increases somewhat and becomes independent of shear rate again at high shear rates. There is no detectable temperature dependence of flow behavior at 170° and 175°C. Irregularly shaped extrudates were obtained at higher shear rates. At constant shear rate the irregularity increased with the length of the capillary. The effect of thermal-mechanical history on the particulate and crystalline structure is discussed with possible influence on the reproducibility of the rheological data. 相似文献