Successful engraftment of allogeneic CD34-enriched marrow cell transplantation from HLA-mismatched parental donors

The CD34 antigen is expressed on pluripotent stem cells and the CD34+ cell has been shown to be capable of hematopoietic reconstitution in animal and human autologous grafts. We asked if CD34+ cells could reconstitute hematopoiesis in human allogeneic transplantation from a HLA-mismatched donor. Three pediatric patients with advanced leukemia received allogeneic CD34-enriched marrow cell graft from HLA two (two patients) or three (one patient) loci-mismatched parental donors. CD34+ cell selection was performed with mouse anti-CD34 antibody 9C5 and magnetic beads coated sheep anti-mouse IgG1. 1.53 to 2.48 x 10(9) marrow cells were processed and 2.53 to 7.89 x 10(7) positively selected cells were recovered. The selected population showed 93.7 to 99.0% CD34+ cells and total recovery of CD34+ cells from the starting population was 54.6 to 62.3%. CD34+ cell selection resulted in more than 99.9% depletion of CD5+ cells from the bone marrow. The patients received 2.53 to 7.25 x 10(6) CD34-enriched cells/kg after myeloablative therapy. All patients achieved trilineage engraftment that was confirmed by various genetic markers. Acute graft-versus-host disease (GVHD) was grade 0 (two patients) or grade I (one patient), and hematological recovery was successfully achieved as follows; the days to reach granulocytes over 0.5 x 10(9)/I were 11 to 13 days, reticulocytes over 2% was 18 to 28 days, platelets over 50 x 10(9)/I was 33 to 58 days. One patient is surviving without relapse of leukemia and two patients died after either mixed hematopoietic chimerism or leukemia relapse was observed. These studies suggest that CD34+ marrow cells are capable of hematopoietic reconstitution from HLA two or three loci-mismatched donors even with the lowest dose of mature T cells.     

Photoreaction of a ZnO gel film chemically modified with β-diketones

Chelate formation is confirmed by a red shift of the n * absorption peak of benzoylacetone (BzAcH) from 309 to 336 nm with its addition to a sol containing Zn²⁺ ions. The chelate bonds between Zn²⁺ and BzAc^– are mostly maintained in the gel film prepared from the sol. Irradiation of the gel film by a Xe lamp with a cut filter (_ex > 300 nm) in the presence of H₂O leads to decomposition of the chelate ring. As a result of the photolysis, ZnO–H groups, CH₃CHO and other carbonyl compounds are generated with the lost of CH₃ groups of the BzAc^– ligand and H₂O involved in the film. INDO/S calculations on a model complex (Zn(BzAc)(OEt)) assign the n * absorption to the electronic transition from a non-bonding molecular orbital (MO) distributed mainly at the phenyl group to an anti-bonding MO localized at the CO bonds. On the basis of these results, a photo-induced hydrolysis mechanism was presented to explain the formation of positive-type patterned ZnO films.     

Glucose transporter levels in a male spontaneous non-insulin-dependent diabetes mellitus rat of the Otsuka Long-Evans Tokushima Fatty strain

Otsuka Long-Evans Tokushima Fatty (OLETF) rats are a new strain of spontaneous non-insulin-dependent diabetes mellitus (NIDDM) models. To evaluate the role of glucose transporters (GLUT) in the development of diabetes in this model, we examined the action of insulin on the translocation of GLUT4 and GLUT1 in isolated adipocytes, and the GLUT4 protein levels in muscles. Long-Evans Tokushima Otsuka (LETO) rats were used as a control strain. In adipocytes, the GLUT4 protein levels in OLETF rats at 30 weeks of age (diabetic stage) were considerably lower than those in LETO rats at the same age. At a pre-diabetic stage (7 weeks), there were no significant differences in GLUT4 protein levels in adipocytes between LETO and OLETF rats. However, the degree of GLUT4 translocation in OLETF rats was lower than that in LETO rats at 7 weeks of age. There were no differences in GLUT1 levels in adipocytes between the two strains. In muscles, the decrease in GLUT4 protein was observed in OLETF rats at 30 weeks of age. Whether such a difference is under the influence of hyperglycemia was also examined using rats rendered diabetic by 70% pancreatectomy. OLETF rats aged 7 weeks were subjected to partial pancreatectomy (Px) and sham pancreatectomy (sham). At 4 weeks after surgery, GLUT4 protein levels in adipose tissues and skeletal muscles were determined. GLUT4 decrease was observed for both tissues of hyperglycemic Px rats compared with euglycemic sham. Moreover, we examined the direct effect of glucose on GLUT4 protein using primary cultured adipocytes of OLETF rats at 5 weeks of age. After 7-day culture with normal (5.6 mmol/l) or high (25 mmol/l) concentrations of glucose, the GLUT4 protein levels in adipocytes decreased at 25 mmol/l glucose compared with 5.6 mmol/l glucose. These findings suggest an early defect in the insulin resistance of OLETF rats probably reflects impaired GLUT4 translocation. The GLUT4 decrease, which occurs later in the process appears to be a consequence, rather than a cause of diabetes in OLETF rats.     

Carbon deposition by electrolytic heating of a water-ethanol solution

A new approach was undertaken to deposit carbon from organic solutions. The method consisted of discharge-heating a tungsten cathode in a water-ethanol electrolyte under a high d.c. voltage. At high temperatures the cathode was covered with a sheath of vapour. In the low voltage region, no carbon deposition occurred. Instead, an uneven surface with spikes of tungsten or a relatively smooth surface, which evolved with increasing voltage, were observed. High voltages and high concentrations of ethanol were favourable for the deposition of bulky carbon and also for the carburization of tungsten to WC. Glassy carbon and disordered graphitic carbon were obtained at the highest applied voltages.     

Barotolerance of Staphylococcus aureus is increased by incubation at below 0 degrees C prior to hydrostatic pressure treatment

The effect of preincubation under low temperatures on inactivation of Staphylococcus aureus IFO 13276 by hydrostatic pressure treatment (HPT) was investigated. Preincubation before HPT was carried out by submerging cell suspension in an ethylene glycol bath at temperatures from 30 to −20 °C for 15 min. After HPT at the same temperatures, survivors of incubated S. aureus was not significantly (P>0.05) influenced when preincubation took place at temperatures above 0 °C. Survivors of incubated S. aureus, however, were approximately two log cycles higher when preincubation took place at temperatures below 0 °C. This increase in barotolerance of S. aureus was not observed in the presence of 40 μg/ml of chloramphenicol.     

A mathematical model of pacemaker activity recorded from mouse small intestine

The pacemaker activity of interstitial cells of Cajal (ICCs) has been known to initiate the propagation of slow waves along the whole gastrointestinal tract through spontaneous and repetitive generation of action potentials. We studied the mechanism of the pacemaker activity of ICCs in the mouse small intestine and tested it using a mathematical model. The model includes ion channels, exchanger, pumps and intracellular machinery for Ca2+ regulation. The model also incorporates inositol 1,4,5-triphosphate (IP3) production and IP3-mediated Ca2+ release activities. Most of the parameters were obtained from the literature and were modified to fit the experimental results of ICCs from mouse small intestine. We were then able to compose a mathematical model that simulates the pacemaker activity of ICCs. The model generates pacemaker potentials regularly and repetitively as long as the simulation continues. The frequency was set at 20 min(-1) and the duration at 50% repolarization was 639 ms. The resting and overshoot potentials were -78 and +1.2 mV, respectively. The reconstructed pacemaker potentials closely matched those obtained from animal experiments. The model supports the idea that cyclic changes in [Ca2+]i and [IP3] play key roles in the generation of ICC pacemaker activity in the mouse small intestine.     

Animated human agents with motion planning capability for 3D-space postural goals

In this paper, we present a rule-based heuristic method of motion planning for an animated human agent with massively redundant degrees of freedom. It constructs motion plans to achieve 3D-space goals of control points on the body, e.g. a hand, while avoiding collisions. Like the artificial potential field approach, the method performs motion decisions in 3D world space rather than in joint space. To handle the massively redundant degrees of freedom, we use a qualitative kinematic model, which specifies motions of body parts and dependencies among them, without specifying the exact distance parameters. This model helps the body select appropriate primitive motions for given goals of control points more globally than does the gradient vector of an artificial potential field of the body. The method simulates (in imagination) the suggested plan to find whether some body parts hit objects, and how much they penetrate the objects. Based on this simulated collision information, the method suggests intermediate goals of the collision body parts. A subplan to achieve these intermediate goals is again postulated by using the qualitative kinematic model. This explicit reasoning helps alleviate the basic cause of local minima in the potential field approach, namely, conflicts between attractive potential fields due to goal positions of control points and repulsive potential fields due to obstacles.     

Automatic background music generation based on actors' mood and motions

Since adding background music and sound effects even to short animations is not simple, an automatic music generation system would help improve the total quality of computer generated animations. This paper describes a prototype system which automatically generates background music and sound effects for existing animations. The inputs to the system are music parameters (mood types and musical motifs) and motion parameters for individual scenes of an animation. Music is generated for each scene. The key for a scene is determined by considering the mood type and its degree, and the key of the previous scene. The melody for a scene is generated from the given motifs and the chord progression for the scene which is determined according to appropriate rules. The harmony accompaniment for a scene is selected based on the mood type. The rhythm accompaniment for a scene is selected based on the mood type and tempo. The sound effects for motions are determined according to the characteristics and intensity of the motions. Both the background music and sound effects are generated so that the transitions between scenes are smooth.