Faults and Claims about Thermal Environments in Relation to Energy Saving Measures in Smaller Office Buildings

Building equipment, energy-saving systems, and claims of inappropriate indoor thermal environments were analyzed in relation to the floor area using responses to a questionnaire survey of service managers of 157 buildings built in Osaka, Kyoto and Hyogo prefectures in Kinki area of Japan. Results show the following： （1） In smaller buildings （〈 5,000 m2）, setting temperatures are higher in summer and lower in winter, effects of ＂uncomfortable radiation from windows＂ are greater, energy-saving systems decrease indoor thermal comfort, but claims of ＂hot＂ and ＂cold＂ are fewer; （2） Claims of ＂hot＂ and ＂cold＂ are unrelated to the setting temperature and whether the air-conditioning control system is central or local; （3） The adoption rates of mitigation of dress codes （＂COOL-BIZ＂ and ＂WARM-BIZ＂） are higher than those of temperature mitigation of air conditioning.     

Pharmaceutical quantification with univariate analysis using transmission Raman spectroscopy

The purpose of this study was to investigate the quantification performance of transmission Raman spectroscopy with univariate analysis. Model dosage forms containing acetaminophen and an excipient, lactose monohydrate, were prepared. The Raman spectra of the tablets were obtained using the modes of transmission, backscattering micro-spectroscopy, and wide area illumination. Calibration curves for quantification of acetaminophen in the tablets were created using peak heights of the Raman spectra. Of the three modes of measurement, the quantitative results by transmission had the highest correlation with those by conventional UV–vis methods. In the validation of quantification by the transmission mode with univariate analysis, a certain degree of daily variation was confirmed. Additionally, quantitative results using peak heights were compared with those of partial least squares (PLSs) multivariate analysis. The root mean square error of prediction (RMSEP) suggested that quantification using PLS provided better precision than the peak height method as expected. However, content uniformity test using large sample sizes by the Raman spectra is not required to be very highly predictive because they usually employ non-parametric criteria and include wide specification ranges. Therefore, univariate analysis using transmission Raman spectroscopy was a suitable quantitative method for conducting content uniformity tests of large sample sizes.     

Isolation and characterization of murine hepatocytes following collagenase infusion into left ventricle of heart

A method for obtaining mouse hepatocytes by infusing collagenase solution into the left ventricle was established. This technique was shown to be equivalent to the intra-portal infusion method and more practical, especially in postnatal mice with a small body size.     

Three-layer organic solar cell with high-power conversion efficiency of 3.5%

To prepare organic solar cells with practical level of energy-conversion efficiency, the following strategies were adopted. By using HD as the photosensitizer, which is heterodimer consisting of a weak electron-donating 5, 10, 15, 20-tetra(2, 5-dimethoxyphenyl)porphyrinatozinc and a weak electron-accepting 5, 10, 15-triphenyl-20-(3-pyridyl)porphyrin, intramolecular photoinduced electron transfer is promoted resulting in effective charge separation. To create an energetically well-arranged system, the HD was placed between an electron-acceptor layer of PV (perylene-3, 4, 9, 10-tetracarboxyl-bis-benzimidazole) and strong electron-donor layer of MC (3-carboxymethyl-5-[(3-ethyl-2(3H)-benzothiazolylidine)ethylidene]-2-thioxo-4-thiazolidinone), where photoinduced intermolecular electron transfer from HD to PV and rapid injection of electrons from MC to HD suppress back electron transfer in the charge-separated HD. As a result of this, the three-layer solar cell Al/PV/HD/MC/Au showed fairly good photovoltaic properties, short-circuit photocurrent quantum yield of 49.2%, open-circuit photovoltage of 0.39 V, fill factor of 0.51, and energy conversion yield of 3.51% when irradiated with 445 nm monochromatic light of 12 μW cm⁻² intensity transmitted through the Al/PV interface. Since the photocurrent hardly decreased with age, the photocurrent observed here is really from energy conversion and not from photocorrosion of Al electrode being occasionally responsible for the photocurrent.     

Isolation and characterization of novel lipases from a metagenomic library of the microbial community in the pitcher fluid of the carnivorous plant Nepenthes hybrida

Members of the genus Nepenthes are carnivorous plants that use the pitfall method of insect capture as a supplementary nutritional source. We extracted metagenomic DNA from the microbial community found in the pitcher fluid of Nepenthes and constructed a plasmid-based metagenomic library. An activity-based screening method enabled the isolation of two lipase genes, lip1 and lip2. Both Lip1 and Lip2 belong to a novel family or subfamily of lipases and show lipase activities in acidic conditions, such as those found in pitcher fluid. This study was conducted under the assumption that the secreted Lip1 and Lip2 were capable of enzymatic activity in the acidic pitcher fluid.     

Phase separation of polymerized lipids in hybrid liposomes

Summary Hybrid liposomes were prepared from the mixture of dipalmitoyl phosphatidylcholine (DPPC) and di-2,4-octadecadiene phosphatidylcholine (DODPC), and their phase separation behaviours was analyzed. In hybrid liposome systems, two transition temperatures (18 and 41°C for DODPC and DPPC, respectively) were clearly observed by means of DSC and fluorescence techniques. These two transition temperatures were also found after polymerization of DODPC molecules in hybrid liposomes by the UV irradiation. These strongly suggest that DPPC and DODPC molecules do not dissolve well but form clusters in the membrane. This phase separation was directly confirmed by freeze-fracture TEM technique.     

Resonant ion stimulated desorption of O from the TiO2(1 1 0) surface: Effects of oxygenation and ion bombardment

The O⁺ desorption from reduced, oxygenated, and ion-bombarded TiO₂(1 1 0) surfaces has been investigated during He⁺ irradiation. The O⁺ desorption is initiated by creation of an antibonding O 2s core hole state via quasi-resonant charge exchange with the He⁺ 1s state, followed by the intra-atomic Auger decay of the O 2s hole. Upon oxygenation of the reduced TiO₂(1 1 0) surface, the O⁺ yield increases by one order of magnitude. The O₂ molecule is dissociated at the vacancy site of bridging oxygen and the oxygen atoms either fill a vacancy site or chemisorb at a fivefold-coordinated Ti⁴⁺ site as an adatom. The latter is detected with much higher efficiency than the former. The O⁺ yield is increased during He⁺ bombardment of the reduced TiO₂(1 1 0) surface due to formation of lower coordinated oxygen atoms. The oxygen species thus formed by ion bombardment or oxygenation are unstable on the surface and tend to diffuse into bulk vacancy sites or higher coordination surface sites even at room temperature.     

Effects of Wnt-10b on proliferation and differentiation of adult murine skin-derived CD34 and CD49f double-positive cells

Although mouse Wnt-10b has been shown to play various roles in a wide range of biological actions, the effects on epithelial stem/progenitor cells in the skin have not been reported. In the present study, we investigated the effects of Wnt-10b on proliferation and differentiation of murine skin-derived CD34 and CD49f double-positive (CD34⁺CD49f⁺) cells, a supposed fraction as enriched epithelial stem/progenitor cells. The cells were prepared from dorsal skin samples obtained from young adult mice as α6 integrin (CD49f) and CD34 double-positive cells by fluorescent activated cell sorting (FACS), and they were cultured with or without Wnt-10b to investigate its effects on proliferation and differentiation. Involvement of canonical Wnt signaling pathway was confirmed by TOPFLASH assay, and differentiation of the CD34⁺CD49f⁺ cells was assessed by RT-PCR analysis and immunocytochemical examinations. The skin-derived CD34⁺CD49f⁺ cells were immunopositive for Lhx2 and expressed mRNA of classical markers for bulge stem cells, including Lhx2, keratin15, Sox9, S100a6, and NFATc1. Their proliferation was suppressed by Wnt-10b, and the markers for differentiated epithelial cells became to be expressed in the culture with Wnt-10b. These results suggest that Wnt-10b promotes differentiation of epithelial stem/progenitor cells in the skin.