Release kinetics of antioxidant compounds from Hibiscus sabdariffa L. encapsulated in gelatin beads and coated with sodium alginate

Gelatin beads containing a concentrated extract of Roselle (Hibiscus sabdariffa L.) calyx rich in polyphenolic compounds were coated with sodium alginate and ionotropically gelled using CaCl₂. Single‐coated beads and double‐coated beads were obtained by this technique, and the release pattern of the loaded extract was evaluated. As a result, release pattern of these compounds fits properly to a first–order Weibull distribution equation. The release rate constant decreased linearly with the number of alginate coats and with the increase in immersion time in CaCl₂ and the Lag period increased significantly with the number of alginate coats. The release of H. sabdariffa's polyphenols can be well controlled manipulating the number of alginate coats and the immersion time in a CaCl₂ solution, allowing not only to control the gastrointestinal segment where they could be released but also to control the release rate with the certainty that the initial concentration will be completely released showing a highly significant antioxidant activity as well.     

A study on the texture diversity of the Artisan Ranchero Cheese from Central Mexico

The objective of the present study was to determine the diversity and causative factors in the textural profile of the Artisan Ranchero Cheese (ARC), a popular soft cheese made with raw cow's milk, which is consumed fresh. These factors included climatic conditions, production zone, cheese supplies, cheesemaking procedure and chemical attributes. Texture attributes were determined using texture profile analysis method. The relationship between texture and chemical attributes of the ARC were determined by a Factor Analysis. An Agglomerative Hierarchical Clustering Analysis was used to differentiate the ARC manufactures into groups. Two groups were identified, production zone being the variable which explained the variation in the data. Finally, a Discriminant Analysis was performed to determine standardisation of the ARC attributes through the sampling periods. The 89% of cheese samples were correctly classified into their own cheese manufacture. This last analysis demonstrated that there was an important degree of standardisation of the cheesemaking procedure within each cheese manufacture, something that was not demonstrated before with artisan cheesemakers.     

Nixtamalised flour and tortillas from transgenic maize (Zea mays L.) expressing amarantin: Technological and nutritional properties

Nixtamalised flour from transgenic maize (genetically modified maize with the cDNA of amarantin) and its tortillas were evaluated for some technological and nutritional properties and compared to the commercial brand MASECA^™. Nixtamalised transgenic maize flour (NTMF) showed higher protein content, total colour difference, pH, water solubility index, essential amino acids content and lower Hunter “L” value, water absorption index, resistant starch and retrograded resistant starch than MASECA^™ flour. Tortillas from NTMF had higher protein content (12.64% vs 8.93%, db), essential amino acids content and calculated protein efficiency ratio (C-PER; 2.05 vs 1.04) than tortillas from MASECA^™. Tortillas from both nixtamalised transgenic maize and MASECA^™ flours showed similar sensory properties (puffing and acceptability). The use of transgenic maize for flour and tortilla preparation may have a positive impact on the nutritional status of people from countries where maize is the basic staple food.     

Microbial ecology of a lactate-driven dark fermentation process producing hydrogen under carbohydrate-limiting conditions

Despite the high prevalence of lactic acid bacteria in dark fermentation (DF) processes, their ecological role is not yet completely elucidated, preventing their systematic use as “helpers” for hydrogen production. The aim of this study was to investigate the microbial community structure of a lactate-driven DF process that successfully produced hydrogen under carbohydrate-limiting conditions using tequila vinasse as a substrate. Microbial responses to stepwise decreases in hydraulic retention time (HRT) from 24 to 4 h were assessed by using Illumina MiSeq sequencing. HRTs above 12 h and below 6 h led to a lower hydrogen production rate (HPR; 0.2–3.3 L/L-d) and process stability (HPR variations within 25–65%), which were associated with the presence of Acetobacter lovaniensis, Clostridium luticellari, Blautia coccoides, and the high abundance of propionate and lactate. Interestingly, transient conditions from unsteady-to-steady state occurred at an HRT of 12 h, where species richness and evenness decreased remarkably. Accordingly, HRTs between 12 and 6 h resulted in higher HPRs of up to 11.7 ± 0.7 L/L-d with HPR variations of less than 10%, which closely matched with the dominance of Clostridium sp., and butyrate and acetate as the main aqueous products. Overall, the results indicate that the successfulness of exploiting the ‘unwanted’ LAB proliferation through lactate-driven DF processes requires the enrichment of lactate-consuming and hydrogen-producing bacteria, which entails the selection of proper biocatalysts and operating conditions/strategies such as the operation of DF reactors under carbohydrate-limiting conditions and low HRTs.     

Cover Image,Volume 68, Issue 5

The cover image is based on the Research Article Effect of different compatibilizers on environmentally friendly composites from poly(lactic acid) and diatomaceous earth by Angel Aguero et al. DOI: 10.1002/pi.5779 .

     

The Effect of Exogenous Jasmonic Acid on Induced Resistance and Productivity in Amaranth (Amaranthus hypochondriacus) Is Influenced by Environmental Conditions

Amaranthus hypochondriacus is a C4 pseudocereal crop capable of producing reasonable grain yields in adverse environmental conditions that limit cereal performance. It accumulates trypsin inhibitors and alpha-amylase inhibitors in seeds and leaves that are considered to act as insect feeding deterrents. Foliar trypsin and alpha-amylase inhibitors also accumulate by treatment with exogenous jasmonic acid (JA) in controlled laboratory conditions. Three field experiments were performed in successive years to test if two nonphytotoxic dosages of JA were capable of inducing inhibitor activity in A. hypochondriacus in agronomical settings, and if this induced response reduced insect herbivory and insect abundance in foliage and seed heads. The performance of JA-treated plants was compared to insecticide-treated plants and untreated controls. The effect of exogenous JA on the foliar levels of six additional putatively defence proteins was also evaluated. Possible adverse effects of JA induction on productivity were evaluated by measuring grain yield, seed protein content, and germination efficiency. The results present a complex pattern and were not consistent from year to year. To some extent, the yearly variability observed could have been consequence of growth under drought versus nondrought conditions. In a drought year, JA-treated plants had lower levels of insect herbivory-derived damage in apical leaves and panicle than control plants, whereas in nondrought years, there was an inconsistent effect on aphids, with no effect on lepidopteran larvae. JA treatments reduced the size of the insect community in seed heads. The effect varied with year. Exogenous JA did not adversely affect productivity, and in the absence of drought stress, the higher dosage enhanced grain yield. Induction of defensive proteins by JA, although sporadic, was more effective in nondrought conditions. The patterns of foliar protein accumulation observed suggest that they may be part of a constitutive, rather than inducible, chemical defense mechanism that is developmentally regulated and critically dependent on the environment. The results emphasize the difficulties that are often encountered when evaluating the performance of chemical elicitors of induced resistance in field settings.     

Corruptible social agents

Agent‐based virtual simulations of social systems susceptible to corruption (e.g., police agencies) require agents capable of exhibiting corruptible behaviors to achieve realistic simulations and enable the analysis of corruption as a social problem. This paper proposes a formal belief‐desire‐intention framework supported by the functional event calculus and fuzzy logic for modeling corruption based on the integrity level of social agents and the influence of corrupters on them. Corruptible social agents are endowed with beliefs, desires, intentions, and corrupt‐prone plans to achieve their desires. This paper also proposes a fuzzy logic system to define the level of impact of corruption‐related events on the degree of belief in the truth of anti‐corruption factors (e.g., the integrity of the leader of an organization). Moreover, an agent‐based model of corruption supported by the proposed belief‐desire‐intention framework and the fuzzy logic system was devised and implemented. Results obtained from agent‐based simulations are consistent with actual macro‐level patterns of corruption reported in the literature. The simulation results show that (i) the bribery rate increases as more external entities attempt to bribe agents and (ii) the more anti‐corruption factors agents believe to be true, the less prone to perpetrate acts of corruption. Copyright © 2014 John Wiley & Sons, Ltd.     

Removing τ‐fluvalinate residues from press‐extracted honey

Honey is obtained from the hives of European honeybees. Honeybees can be infested by Varroa parasitism, which affects honey production. The acaricide τ‐fluvalinate used in beehives to control Varroa mites, can leave its residues in honey, pollen and wax. A monitoring study was undertaken to determine the τ‐fluvalinate residues in honey samples taken from treated hives. The τ‐fluvalinate residues were determined by gas chromatography using a short, non‐polar packed column with electron capture detection. Analysis of 50 samples showed an average residue level of 15 µg kg⁻¹. The same samples were filtered through Whatman filter paper to separate the honey from residual suspended particles. The filtered honeys showed no acaricide residues above the detection limit of 3 µg kg ⁻¹, while the average residue level determined in the sediments was 392 µg kg ⁻¹. Thus the residues of τ‐fluvalinate in honey can be eliminated by separating the honey from residual suspended particles that contain acaricide residues. Copyright © 2003 Society of Chemical Industry