The use of noninvasive biological means in assessing lipids in children

Gas chromatographic analysis of the lipid fatty acid spectrum of blood (serum and plasma), sweat, and exhaled air condensate in children with acute pneumonia and dermatitis caused by alimentary allergies showed good correlation of the results. Therefore, biological objects obtained by noninvasive methods can be used for studies of lipid metabolism in children.     

Possiblity of using immobilized urease for degradation of urea in blood plasma

As revealed the velocities of urea decomposition in the citrate donor plasma with soluble urease and urease immobilized by addition to carboxymethyl ester of cellulose, 2-(3'-amino-4'-methoxyphenyl)-sulphonylethyl ester of cellulose, diethylaminoester of cellulose, stained with dichlortriazine stain, or graft copolymere of cellulose and polyglycidylmetacrylate were sufficiently close to one another. Preparations of immobilized urease can be repeatedly used for urea decomposition in the citrate donor blood. Periodical treatment of the mentioned preparations with cystein solution led to a lesser decrease of enzymatic activity of the immobilized urease after repeated use.     

Dynamics of development of the interstitial tissue and seminiferous tubules in the testis of human fetuses

Morphological changes in the testis of human fetuses (period of embryogenesis--from 8 to 28 weeks) during their differentiation were studied by histological and morphological methods. Investigations carried out demonstrated that complete cycle of transformation in the intestitial and embryonic tissue of the fetal testis could be divided into two main periods: the first period was attended by a gradual increase in the count and differentiation of Heydig's cells in the interstitial tissue (from the 8th to the 12th week of embryogenesis); gradual involution of Leydig's cells in the interstitial tissue and development of seminiferous tubules prevailed during the second period (from the 15th to the 28th week of embryogenesis). In the course of prenatal development of the human fetus testis Leydig's cells passed through a number of successive stages, each one being characterized by a definite functional activity level of these cells. The detected changes in the testes were apparently connected both with the changes in the Leydig's cells count and their functional activity, and with progressive growth of the tubules.     

Nuclear morphometry of the myocardial cells as a diagnostic tool in cases of sudden death due to coronary thrombosis

One general signalling mechanism used to transfer the information delivered by agonists into appropriate intracellular compartments involves the rapid redistribution of ionised calcium throughout the cell, which results in transient elevations of the cytosolic free Ca2+ concentration. Various physiological stimuli increase [Ca2+]i transiently and, thereby, induce cellular responses. However, under pathological conditions, changes of [Ca2+]i are generally more pronounced and sustained. Marked elevations of [Ca2+]i activate hydrolytic enzymes, lead to exaggerated energy expenditure, impair energy production, initiate cytoskeletal degradation, and ultimately result in cell death. Such Ca(2+)-induced cytotoxicity may play a major role in several diseases, including neuropathological conditions such as chronic neurodegenerative diseases and acute neuronal losses (e.g. in stroke).     

Esophagogastrostomy with the EEA stapler

Esophagogastric anastomosis was performed with the EEA stapler in 31 patients who underwent esophageal resections. Anastomoses were accomplished at all levels of the thoracic and cervical esophagus by a variety of approaches. Routine barium cine-esophagograms obtained at seven days after operation failed to demonstrate an anastomotic leak in any patient. The operative mortality rate was 3% (1 of 31 patients). Technical problems occurred during the operation in three patients; in two of these an incomplete anastomosis may have resulted from the surgeon's error. All patients were able to swallow normally at the time of discharge. Late anastomotic stricture occurred in five patients, and responded to dilatation in all but one patient who had local tumor recurrence. We conclude that the EEA stapler allows rapid and reliable esophagogastric anastomosis. Successful use of the instrument requires strict attention to technical detail and awareness of possible pitfalls.