Larissa Midiane Tdero  Carem Gledes Vargas Rechia  Luis Henrique Souza Guimares 《Journal of Food Biochemistry》2019,43(8)

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Sara de Souza Comapa  Luciedry Matheus Souza Carvalho  Carlos Victor Lamarão  Francisca das Chagas do Amaral Souza  Jaime Paiva Lopes Aguiar  Laiane Souza da Silva  Josiana Moreira Mar  Edgar Aparecido Sanches  Felipe Faccini dos Santos  Jaqueline de Araújo Bezerra  Pedro Henrique Campelo 《Journal of Food Processing and Preservation》2019,43(7):e13989

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Ultrasound-assisted homogenization and gum Arabic combined to physicochemical quality of cupuaçu juice     

Larissa Fernanda Rodrigues da Silva  Anice da Silva Gomes  Débora Raquel Gomes Castro  Francisca das Chagas do Amaral Souza  Josiana Moreira Mar  Laiane Souza da Silva  Edgar Aparecido Sanches  Jaqueline de Araújo Bezerra  Amr M. Bakry  Pedro Henrique Campelo 《Journal of Food Processing and Preservation》2019,43(9):e14072

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Liliane de Souza Pires  Katieli Martins Todisco  Natália Soares Janzantti  Maria Aparecida Mauro 《Journal of Food Processing and Preservation》2019,43(10):e14133

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Nadya Panagides  Lucia F. Zacchi  Mitchell J. De Souza  Rodrigo A. V. Morales  Alexander Karnowski  Mark T. Liddament  Catherine M. Owczarek  Stephen M. Mahler  Con Panousis  Martina L. Jones  Christian Fercher 《International journal of molecular sciences》2022,23(15)

Monoclonal antibodies (mAbs) are one of the most successful and versatile protein-based pharmaceutical products used to treat multiple pathological conditions. The remarkable specificity of mAbs and their affinity for biological targets has led to the implementation of mAbs in the therapeutic regime of oncogenic, chronic inflammatory, cardiovascular, and infectious diseases. Thus, the discovery of novel mAbs with defined functional activities is of crucial importance to expand our ability to address current and future clinical challenges. In vitro, antigen-driven affinity selection employing phage display biopanning is a commonly used technique to isolate mAbs. The success of biopanning is dependent on the quality and the presentation format of the antigen, which is critical when isolating mAbs against membrane protein targets. Here, we provide a comprehensive investigation of two established panning strategies, surface-tethering of a recombinant extracellular domain and cell-based biopanning, to examine the impact of antigen presentation on selection outcomes with regards to the isolation of positive mAbs with functional potential against a proof-of-concept type I cell surface receptor. Based on the higher sequence diversity of the resulting antibody repertoire, presentation of a type I membrane protein in soluble form was more advantageous over presentation in cell-based format. Our results will contribute to inform and guide future antibody discovery campaigns against cell surface proteins.  相似文献   

    

Daniela Alexandre  Bernardo Teixeira  Andr Rico  Salete Valente  Ana Craveiro  Pedro V. Baptista  Carla Cruz 《International journal of molecular sciences》2022,23(6)

Lung cancer (LC) is the leading cause of cancer-related death worldwide. Although the diagnosis and treatment of non-small cell lung cancer (NSCLC), which accounts for approximately 80% of LC cases, have greatly improved in the past decade, there is still an urgent need to find more sensitive and specific screening methods. Recently, new molecular biomarkers are emerging as potential non-invasive diagnostic agents to screen NSCLC, including multiple microRNAs (miRNAs) that show an unusual expression profile. Moreover, peripheral blood mononuclear cells’ (PBMCs) miRNA profile could be linked with NSCLC and used for diagnosis. We developed a molecular beacon (MB)-based miRNA detection strategy for NSCLC. Following PBMCs isolation and screening of the expression profile of a panel of miRNA by RT-qPCR, we designed a MB targeting of up-regulated miR-21-5p. This MB 21-5p was characterized by FRET-melting, CD, NMR and native PAGE, allowing the optimization of an in-situ approach involving miR-21-5p detection in PBMCs via MB. Data show the developed MB approach potential for miR-21-5p detection in PBMCs from clinical samples towards NSCLC.  相似文献   

    

Maria Barilli  Maria Cristina Tavera  Serafina Valente  Alberto Palazzuoli 《International journal of molecular sciences》2022,23(9)

One of the most important diagnostic challenges in clinical practice is the distinction between pulmonary hypertension (PH) due to primitive pulmonary arterial hypertension (PAH) and PH due to left heart diseases. Both conditions share some common characteristics and pathophysiological pathways, making the two processes similar in several aspects. Their diagnostic differentiation is based on hemodynamic data on right heart catheterization, cardiac structural modifications, and therapeutic response. More specifically, PH secondary to heart failure with preserved ejection fraction (HFpEF) shares features with type 1 PH (PAH), especially when the combined pre- and post-capillary form (CpcPH) takes place in advanced stages of the disease. Right ventricular (RV) dysfunction is a common consequence related to worse prognosis and lower survival. This condition has recently been identified with a new classification based on clinical signs and progression markers. The role and prevalence of PH and RV dysfunction in HFpEF remain poorly identified, with wide variability in the literature reported from the largest clinical trials. Different parenchymal and vascular alterations affect the two diseases. Capillaries and arteriole vasoconstriction, vascular obliteration, and pulmonary blood fluid redistribution from the basal to the apical district are typical manifestations of type 1 PH. Conversely, PH related to HFpEF is primarily due to an increase of venules/capillaries parietal fibrosis, extracellular matrix deposition, and myocyte hypertrophy with a secondary “arteriolarization” of the vessels. Since the development of structural changes and the therapeutic target substantially differ, a better understanding of pathobiological processes underneath PH-HFpEF, and the identification of potential maladaptive RV mechanisms with an appropriate diagnostic tool, become mandatory in order to distinguish and manage these two similar forms of pulmonary hypertension.  相似文献   

    

Filipa Amaro  Carolina Pisoeiro  Maria Joo Valente  Maria de Lourdes Bastos  Paula Guedes de Pinho  Mrcia Carvalho  Joana Pinto 《International journal of molecular sciences》2022,23(17)

Sunitinib and pazopanib are tyrosine kinase inhibitors (TKIs) used as first-line therapy for metastatic renal cell carcinoma (RCC). Although these TKIs are associated with similar survival outcomes, some differences have been reported in their safety profiles. In this work, traditional toxicological endpoints (cell viability and growth, oxidative stress, and nuclear morphology) and ¹H NMR spectroscopy-based metabolomics analysis were used to provide new insights into the cytotoxicity and metabolic mechanisms underlying sunitinib and pazopanib treatments. Tumoral (Caki-1) and non-tumoral (HK-2) human renal cells were exposed to clinically relevant concentrations of sunitinib (2 µM) or pazopanib (50 µM). Sunitinib showed selectivity for cancer cells, inhibiting proliferation, and inducing apoptotic death of Caki-1 cells, whereas pazopanib had a similar cytotoxic effect in both tumoral and non-tumoral cells. ¹H-NMR metabolomics unveiled a higher impact of sunitinib on the levels of intracellular metabolites of Caki-1 cells (seven dysregulated metabolites), suggesting dysregulations on amino acid, glutathione and glycerophospholipid metabolisms. In contrast, pazopanib had a higher impact on the levels of extracellular metabolites of Caki-1 cells (seven dysregulated metabolites in culture medium), unveiling alterations on amino acid and energetic metabolisms. In HK-2 cells, sunitinib caused only a minor increase in intracellular isoleucine levels, whereas pazopanib induced several alterations on the intracellular (three dysregulated metabolites) and extracellular (three dysregulated metabolites) compartments suggesting changes on amino acid, glycerophospholipid, and energy metabolisms. Our results demonstrate that these TKIs elicit distinct cellular and metabolic responses, with sunitinib showing better in vitro efficacy against target RCC cells and lesser nephrotoxic potential than pazopanib.  相似文献   

    

Marisa Market  Gayashan Tennakoon  Marlena Scaffidi  David P. Cook  Leonard Angka  Juliana Ng  Christiano Tanese de Souza  Michael A. Kennedy  Barbara C. Vanderhyden  Rebecca C. Auer 《International journal of molecular sciences》2022,23(23)

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Diana Pereira  Tiago Soares Carreira  Nuno Alves  ngela Sousa  Joana F. A. Valente 《International journal of molecular sciences》2022,23(3)

The current worldwide pandemic caused by coronavirus disease 2019 (COVID-19) had alerted the population to the risk that small microorganisms can create for humankind’s wellbeing and survival. All of us have been affected, directly or indirectly, by this situation, and scientists all over the world have been trying to find solutions to fight this virus by killing it or by stop/decrease its spread rate. Numerous kinds of microorganisms have been occasionally created panic in world history, and several solutions have been proposed to stop their spread. Among the most studied antimicrobial solutions, are metals (of different kinds and applied in different formats). In this regard, this review aims to present a recent and comprehensive demonstration of the state-of-the-art in the use of metals, as well as their mechanisms, to fight different pathogens, such as viruses, bacteria, and fungi.  相似文献