Visceral Adipose Tissue Displays Unique Metabolomic Fingerprints in Obesity,Pre-Diabetes and Type 2 Diabetes

Visceral adipose tissue (VAT) metabolic profiling harbors the potential to disentangle molecular changes underlying obesity-related dysglycemia. In this study, the VAT exometabolome of subjects with obesity and different glycemic statuses are analyzed. The subjects (n = 19) are divided into groups according to body mass index and glycemic status: subjects with obesity and euglycemia (Ob+NGT, n = 5), subjects with obesity and pre-diabetes (Ob+Pre-T2D, n = 5), subjects with obesity and type 2 diabetes under metformin treatment (Ob+T2D, n = 5) and subjects without obesity and with euglycemia (Non-Ob, n = 4), used as controls. VATs are incubated in culture media and extracellular metabolite content is determined by proton nuclear magnetic resonance (¹H-NMR). Glucose consumption is not different between the groups. Pyruvate and pyroglutamate consumption are significantly lower in all groups of subjects with obesity compared to Non-Ob, and significantly lower in Ob+Pre-T2D as compared to Ob+NGT. In contrast, isoleucine consumption is significantly higher in all groups of subjects with obesity, particularly in Ob+Pre-T2D, compared to Non-Ob. Acetate production is also significantly lower in Ob+Pre-T2D compared to Non-Ob. In sum, the VAT metabolic fingerprint is associated with pre-diabetes and characterized by higher isoleucine consumption, accompanied by lower acetate production and pyruvate and pyroglutamate consumption. We propose that glucose metabolism follows different fates within the VAT, depending on the individuals’ health status.     

Current advances concerning the most cited metal ions doped bioceramics and silicate-based bioactive glasses for bone tissue engineering

Studies related to biomaterials that stimulate the repair of living tissue have increased considerably, improving the quality of many people's lives that require surgery due to traumatic accidents, bone diseases, bone defects, and reconstructions. Among these biomaterials, bioceramics and bioactive glasses (BGs) have proved to be suitable for coating materials, cement, scaffolds, and nanoparticles, once they present good biocompatibility and degradability, able to generate osteoconduction on the surrounding tissue. However, the role of biomaterials in hard tissue engineering is not restricted to a structural replacement or for guiding tissue regeneration. Nowadays, it is expected that biomaterials develop a multifunctional role when implanted, orchestrating the process of tissue regeneration and providing to the body the capacity to heal itself. In this way, the incorporation of specific metal ions in bioceramics and BGs structure, including magnesium, silver, strontium, lithium, copper, iron, zinc, cobalt, and manganese are currently receiving enhanced interest as biomaterials for biomedical applications. When an ion is incorporated into the bioceramic structure, a new category of material is created, which has several unique properties that overcome the disadvantages of primitive material and favors its use in different biomedical applications. The doping can enhance handling properties, angiogenic and osteogenic performance, and antimicrobial activity. Therefore, this review aims to summarize the effect of selected metal ion dopants into bioceramics and silicate-based BGs in bone tissue engineering. Furthermore, new applications for doped bioceramics and BGs are highlighted, including cancer treatment and drug delivery.     

Effects of enzymatic modification of milk proteins on flavour and textural qualities of set yoghurt

Milk proteins were enzymatically modified, using neutrase and trypsin immobilised on CM‐Sephadex C‐50, to the extent of 3, 4 and 5% degrees of hydrolysis. The neutrase‐treated milk and the trypsin‐treated milk were used separately to prepare set yoghurt. The set yoghurt prepared from neutrase‐treated milk showed an improvement in body, texture and flavour, a faster rate of acid development and a reduction in setting time compared with the control. However, the set yoghurt prepared from trypsin‐treated milk showed either a small or no improvement in textural and sensory properties. © 2000 Society of Chemical Industry     

Antimicrobial effects of chitosans and chitooligosaccharides, upon Staphylococcus aureus and Escherichia coli, in food model systems

The objective of this study was to elucidate the controversial relationship between the molecular weight (MW) of chitosans and their antibacterial activity (upon different inoculum levels, at several concentrations). The influence of food components on the activity was also ascertained, as well as acceptance by a sensory panel. All the compounds tested exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli. This activity was shown to be closely dependent on the inoculum level, MW and concentration used. Within 4h at 10(3) cells/mL, all five compounds, at every concentration (0.5%, 0.25% and 0.1%, w/v), proved to be bactericidal; for higher inocula, 0.1% (w/v) was only bacteriostatic; at 10(7) or 10(5) cells/mL, and independently of the inoculum level, 0.25% (w/v) of any chitooligosaccharide (COS) mixture was sufficient to reduce the E. coli initial population by at least 3 log cycles; COS never exhibited bactericidal action over S. aureus, unlike high and medium MW chitosans-which, at 0.5% (w/v), presented a bactericidal effect even against 10(7) cells/mL. When incorporated in liquid food matrices, medium and high MW chitosans maintained their activity, for both matrices and bacteria, although a slower activity was noticeable in milk; however, COS lost their activity upon both bacteria in milk after 4-8h. Furthermore, addition of chitosans to apple juice led to several unpleasant off-flavors, such as astringency and after taste--which increased in magnitude with MW.     

EuQoS: End-to-End Quality of Service over Heterogeneous Networks

The EuQoS (End-to-End QoS over Heterogeneous Networks) IST Integrated European Project aimed to define a Next Generation Network architecture that builds, uses and manages end-to-end QoS across different administrative domains and heterogeneous networks (UMTS, xDSL, Ethernet, WiFi, Satellite and IP/MPLS). The EuQoS architecture preserves the openness and the decentralized decision model of the actual Internet, runs on off-the-shelf hardware and network equipment, and allows end users to request various services without changing the Application Signaling protocol, while meeting regulators’ and users’ Net Neutrality requirements. This paper presents the key elements of the EuQoS architecture and describes the main results obtained in field trials performed on a fully-functional EuQoS system prototype developed over a pan-European testbed. Furthermore, the paper discusses the main strengths of the system and the issues related to its actually deployment on a large scale, from both technical and market points of view.     

Sustainability metrics for eco-technologies assessment, Part II. Life cycle analysis

This work presents a sustainability analysis of CO₂ reuse to produce dimethyl carbonate (DMC) via ethylene oxide (ROUTE A) and via urea methanolysis (ROUTE B). Two different technologies are considered in ROUTE A: reaction followed by separation and reactive distillation. Life cycle analyses of the ROUTES are presented employing sustainability analysis performed in HYSYS process simulator, along with WAR Algorithm. Process economical optimization is performed to maximize the processes profitability. A sustainability function, defined as a 2D indicator involving both economical and environmental aspects, is calculated for the optimized processes. Additional sustainability indexes are evaluated: material index, energy index, and ecoefficiency. The results, under both economical and environmental aspects, show that ROUTE A is the most sustainable. The study also points to the relevance of the frontier set between the domains cradle-to-gate and gate-to-gate in assessing process sustainability during LCA.     

Analysis of cubic and orthorhombic C<Subscript>3</Subscript>A hydration in presence of gypsum and lime

Field emission scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD) have been used to study the microstructural changes and phase development that take place during the hydration of cubic (pure) and orthorhombic (Na-doped) tricalcium aluminate (C₃A) and gypsum in the absence and presence of lime. The results demonstrate that important differences occur in the hydration of each C₃A polymorph and gypsum when no lime is added; orthorhombic C₃A reacts faster with gypsum than the cubic phase, forming longer ettringite needles; however, the presence of lime slows down the formation of ettringite in the orthorhombic sample. Additional rheometric tests showed the possible effects on the setting time in these cementitious mixes.     

Characterizing the nano and micro structure of concrete to improve its durability

New and advanced methodologies have been developed to characterize the nano and microstructure of cement paste and concrete exposed to aggressive environments. High resolution full-field soft X-ray imaging in the water window is providing new insight on the nano scale of the cement hydration process, which leads to a nano-optimization of cement-based systems. Hard X-ray microtomography images of ice inside cement paste and cracking caused by the alkali–silica reaction (ASR) enables three-dimensional structural identification. The potential of neutron diffraction to determine reactive aggregates by measuring their residual strains and preferred orientation is studied. Results of experiments using these tools are shown on this paper.