Gel-entrapment of perfluorocarbons: a fluorine-19 NMR spectroscopic method for monitoring oxygen concentration in cell perfusion systems

Oxygenation is a major determinant of the physiological state of cultured cells. 19F NMR can be used to determine the oxygen concentration available to cells immobilized in a gel matrix by measuring the relaxation rate (1/T1) of perfluorocarbons (PFC) incorporated into the gel matrix. In calcium alginate gel beads without cells the relaxation rate (1/T1) of the trifluoromethyl group of perfluorotripropylamine (FTPA) varies linearly with oxygen concentration, with a slope of 1.26 +/- 0.15 x 10(-3) s-1 microM-1 and an intercept of 0.50 +/- 0.04 s-1. During perfusion with medium equilibrated with 95%/5% O2/CO2, changes in PFC T1s indicate that the average oxygen concentration was reduced from 894 +/- 102 microM in the absence of cells to 476 +/- 65 microM and 475 +/- 50 microM in the presence of 0.7 x 10(8) EMT6/Ro and RIF-1 murine tumor cells per milliliter of gel, respectively. The presence of 0.2 microliters of FTPA/ml of gel had no effect on the energy status of the cells as indicated by 31P NMR spectra. To calculate oxygen gradients within the beads from the average PFC T1 of the sample, a mathematical model was used assuming that oxygen is the limiting nutrient for cell metabolism and that the cellular oxygen consumption rate is independent of oxygen concentration. Data for EMT6/Ro cells were fit using experimentally determined perfusion parameters together with literature values for cell volume and oxygen consumption rate.(ABSTRACT TRUNCATED AT 250 WORDS)     

Numerical simulation model of vibration responses of rectangular plates embedded with piezoelectric actuators

A numerical simulation model for random large amplitude vibration control of composite plate using piezoelectric material is presented. The H_∞ control design is employed to suppress the large amplitude vibrations of composites plates under random loading. The numerical simulation model is developed and based on the finite element method. The finite element governing equation includes fully coupled structural and electrical nodal degrees of freedom, and consider the von Karman large amplitude vibration. The modal reduction method using the structural modes is adopted to reduce the finite element equations into a set of modal equations with fewer degrees of freedom. The modal equations are then employed for controller design and time domain simulation. In the simulations without control, the value of the linear mode to the nonlinear deflection is quantified; and the minimum number of linear modes needed for accurate model is obtained. In the simulations with control, it is shown that the truncated modes, which are neglected in the control design, deteriorate the controller performance. Generally, the vibration reduction level is not monotonically increasing with the size of the piezoelectric actuator. The optimal piezoelectric actuator size depends on the excitation level. For higher excitation level, optimal actuator size is larger. The H_∞ controller based on the linear finite element formulation gives better vibration reduction for small amplitude vibration, but it still gives reasonable performance for large amplitude vibration provided that the piezoelectric actuator is big and powerful enough.     

Numerical simulation of aerosol collection in filters with staggered parallel rectangular fibres

 In this paper, filters with rectangular fibres arranged in a staggered and parallel array and placed transverse to the flow are studied numerically. A two- dimensional flow field is obtained by solving Navier–Stokes equations with the control volume method. Periodic boundary conditions are introduced in the calculation. In order to achieve higher accuracy, a second-order upwind scheme is adopted and a fine mesh is arranged near the fibre and the symmetrical plane of the flow field where large gradients in velocity are expected. Particle trajectories are calculated by solving the corresponding Lagrangian equation of motion to obtain the collection efficiency of a single rectangular fibre, in which positions of the approaching particles on the inlet plane of the flow field are randomly distributed according to the Monte-Carlo principle. The simulation considers all the important mechanisms of particle capture including interception, inertial impaction and Brownian motion. Effects of fibre aspect ratio, filter packing density, particulate size and Reynolds number on the collection efficiency are numerically determined. The volumetric packing density ranges from 0.4 to 4% and the particle diameter is from 0.01 μm to 2 μm. Reynolds number based on the height of computational domain varies from 20 to 100 and the aspect ratio is from 0.1 to 10. Simulations with and without Brownian motion are carried out for different Reynolds numbers, packing densities and aspect ratios and the results show that Brownian effects are significant for particles smaller than 1 μm. Received 25 May 2001     

A new boundary integral equation for notch problem of plane elasticity

A new boundary integral equation for the notch problem of plane elasticity is formulated in this paper. In the formulation, the distributed dislocation density is taken to be the unknown function and the resultant force function to be the right-hand term in the resulting integral equation. As a result the integral equation derived contains a logarithmic kernel. The equation is compact in form and convenient for computation. The accuracy of the method is demonstrated through a number of numerical examples.     

An electromigration failure model for interconnects under pulsedand bidirectional current stressing

An electromigration failure model which can be used to project the electromigration lifetime under pulsed DC and AC current stressing has been reported. The experimental results indicate that different metallization systems (Al-2%Si, Al4%Cu/TiW, and Cu) show similar failure behaviors, which can be explained and predicted by this model. The pulsed DC lifetime is found to be longer than DC lifetime, and the AC lifetime is found to be very much longer. This recognition can provide significant relief to circuit designs involving metals carrying pulsed DC and AC currents, and allow a more aggressive design to improve circuit density and speed     

Characterization and comparison of PECVD silicon nitride and silicon oxynitride dielectric for MIM capacitors

In this letter, we report on the electrical characteristic and the comparison of the metal-insulator-metal (MIM) capacitors with PECVD silicon nitride (SiN) and silicon oxynitride (SiON). Both capacitors are found to exhibit low leakage and high breakdown field strength, as well as absence of dispersive behavior, good linearity, and comparable quality factor behaviors.     

Field dependence of impact ionization coefficients in In/sub 0.53/Ga/sub 0.47/As

Electron and hole ionization coefficients in In/sub 0.53/Ga/sub 0.47/As are deduced from mixed carrier avalanche photomultiplication measurements on a series of p-i-n diode layers, eliminating other effects that can lead to an increase in photocurrent with reverse bias. Low field ionization is observed for electrons but not for holes, resulting in a larger ratio of ionization coefficients, even at moderately high electric fields than previously reported. The measured ionization coefficients are marginally lower than those of GaAs for fields above 250 kVcm/sup -1/, supporting reports of slightly higher avalanche breakdown voltages in In/sub 0.53/Ga/sub 0.47/As than in GaAs p-i-n diodes.     

The Effect of Moisture Content on the Conversion of Ergosterol to Vitamin D in Shiitake Mushrooms

The distribution of ergosterol in different parts of shiitake mushrooms was studied in order to optimize the conversion of ergosterol to vitamin D₂ during drying. The effect of moisture on the conversion of ergosterol to vitamin D₂ was also investigated by adjusting the moisture content of fresh mushrooms to different levels in a large vacuum desiccator and subjecting them to UV irradiation at 290-320 nm wavelength, for 2 h. The moisture, ergosterol and vitamin D₂ contents were determined using standard procedures. It was found that the ergosterol content on a dry matter basis of fresh shiitake mushroom was highest in the gills (10.6 ± 0.99 mg/g DM), followed by the cap or pileus (5.34 ± 0.64 mg/g DM) and was least in the stalk or stipe (2.97 ± 0.56). The moisture content had a marked influence on the conversion of ergosterol to vitamin D₂, the best conversion taking place at a moisture content of about 70% on a wet basis.     

Apply geometric duality to energy-efficient non-local phenomenon awareness using sensor networks

A powerful concept to cope with resource limitations and information redundancy in wireless sensor networks is the use of collaboration groups to distill information within the network and suppress unnecessary activities. When the phenomena to be monitored have large geographical extents, it is not obvious how to define these collaboration groups. This article presents the application of geometric duality to form such groups for sensor selection and non-local phenomena tracking. Using a dual-space transformation, which maps a non-local phenomenon (e.g., the edge of a half-plane shadow) to a single point in the dual space and maps locations of distributed sensor nodes to a set of lines that partitions the dual space, one can turn off the majority of the sensors to achieve resource preservation without losing detection and tracking accuracy. Since the group so defined may consist of nodes that are far away in physical space, we propose a hierarchical architecture that uses a small number of computationally powerful nodes and a massive number of power constrained motes. By taking advantage of the continuity of physical phenomena and the duality principle, we can greatly reduce the power consumption in non-local phenomena tracking and extend the lifetime of the network.