N‐Acylhomoserine lactones (AHLs) are used by a wide variety of bacteria for cell–cell communication in “quorum‐sensing”. These compounds are derived from L ‐homoserine lactone and a fatty acid, which varies in chain‐length, degree of saturation, and the presence or absence of an oxygen atom at C‐3. In this study we describe for the first time the occurrence of acyl chains carrying a methyl branch, and present a GC‐MS‐based method that can be used to distinguish these compounds from unbranched isomers. The bacterium Aeromonas culicicola produces several methyl branched AHLs. In Jannaschia helgolandensis—a marine bacterium of the Roseobacter clade—a doubly unsaturated AHL, (2E,9Z)‐N‐(2,9‐hexadecadienoyl)‐L ‐homoserine lactone, occurs. The location and configuration of the double bonds was proven by spectrometric investigation and synthesis. Finally, a method was developed to establish the absolute configuration of 3‐hydroxyalkanoyl‐HSLs by mild cleavage and chiral gas chromatography. The AHLs synthesized during this study were tested in sensor systems specific for certain AHL types. The results show that these compounds display varying responses to the respective sensors; this underlines the importance of determining the whole bouquet of AHLs and its function to fully understand their importance for regulatory functions in bacteria.相似文献
Recent developments in nanotechnology provided an opportunity to solve many complex problems in the field of energy. Performance investigation of the nanoscale thermal cycles can prove crucial in the development of efficient and less polluting energy system. Due to the influence of boundary phenomenon and quantum degeneracy effects, a nanoscale engine performs according to statistical quantum thermodynamics instead of classical thermodynamics. In this study, a nanoscale Stirling engine operating on an ideal Maxwell‐Boltzmann gas is investigated for multiobjective optimization. Optimization problem of Stirling cycle is formed considering the thermal efficiency, ecological coefficient of performance and entropy generation. An application example of a nanoscale Stirling engine is presented and solved using Heat Transfer Search algorithm. Maxwell‐Boltzmann gas restricted in a finite domain is studied and the effect of different parameters, such as surface area ratio, volume ratio, and temperature ratio of the domain, is investigated. Sensitivity analysis is carried out to identify the effect of design variables on the performance parameters. Further, influence of the source temperature and the number of particles of working fluid on the objective functions is studied and presented. 相似文献
ABSTRACT Fossil fuel resource is on the draining stage which leads to an increment in the cost of the petroleum products. Nowadays, research is focused on the development of environmental friendly lubricants which are derivatives of renewable sources. Inedible plant oil-driven bio-lubricants are environmentally friendly because they are non-hazardous, biodegradable, as well as there is no emission of toxic gases. This study involves the characterisations, advantages, as well as utilisation of inedible plant oil-driven bio-lubricants as an alternative for tribological applications. This report presents the status about the global lubricant market as well as potential outlook. Inedible plant oil-driven bio-lubricants bear high viscosity, high lubricity, and high viscosity index which can enhance the equipment service life and has the ability to carry high load and results in minimum amount of metal traces during combustion. 相似文献
To ensure highest security in handheld devices, biometric authentication has emerged as a reliable methodology. Deployment of mobile biometric authentication struggles due to computational complexity. For a fast response from a mobile biometric authentication method, it is desired that the feature extraction and matching should take least time. In this article, the periocular region captured through frontal camera of a mobile device is considered under investigation for its suitability to produce a reduced feature that takes least time for feature extraction and matching. A recently developed feature Phase Intensive Local Pattern (PILP) is subjected to reduction giving birth to a feature termed as Reduced PILP (R-PILP), which yields a matching time speed-up of 1.56 times while the vector is 20% reduced without much loss in authentication accuracy. The same is supported by experiment on four publicly available databases. The performance is also compared with one global feature: Phase Intensive Global Pattern, and three local features: Scale Invariant Feature Transform, Speeded-up Robust Features, and PILP. The amount of reduction can be varied with the requirement of the system. The amount of reduction and the performance of the system bears a trade-off. Proposed R-PILP attempts to make periocular suitable for mobile devices. 相似文献
Clean Technologies and Environmental Policy - There is a dire need to replace the chemical buffers that regulate the redox environment in single-stage anaerobic digestion of food waste. Hence, the... 相似文献
Multimedia Tools and Applications - Performance of computerized diagnostic systems yearning to be approved by medical regulatory bodies must meet the expectations of human experts. Highly accurate... 相似文献
Person re-identification which aims at matching people across disjoint cameras has received increasing attention due to the widespread use of video surveillance applications. Existing methods concentrate either on robust feature extraction or view-invariant feature transformation. However, the extracted features suffer from various limitations such as color inconsistency and scale variations. Besides, during matching, a probe is compared against each gallery instance which represents only the pairwise relationship and ignores the high order relationship among them. To address these issues, we propose a multi-shot person re-identification framework that first performs a preprocessing task on images to address illumination variations for maintaining the color consistency. Subsequently, we formulate an approach to handle scale variations in the pedestrian appearances for keeping them with relatively a fixed scale ratio. Overlapped visual patches representing appearance cues are then extracted from the processed images. A structured multi-class feature selection approach is employed to select a set of relevant patches that simultaneously discriminates all distinct persons. These selected patches use a hypergraph to represent the visual association among a probe and gallery images. Finally, for matching, we formulate a hypergraph-based learning scheme, which considers both the pairwise and high-order association among the probe and gallery images. The hypergraph structure is then optimized to yield an improved similarity score for a probe against each gallery instance. The effectiveness of our proposed framework is validated on three public datasets and comparison with state-of-the-art methods shows the superior performance of our framework.
Journal of Failure Analysis and Prevention - A dragline is the largest mobile equipment on earth, and it is called the “kingpin” of any mine site. In this present investigation, a case... 相似文献
In the present study, fatigue and fracture characteristics of sensitized marine grade Al‐Mg (AA 5754) alloy are experimentally evaluated. Received alloy is sensitized at temperatures of 150°C (SENS50) and 175°C (SENS75) for 100 hours. Fracture parameters, KIc and JIc, are experimentally evaluated. Slow strain rate tensile tests at a crosshead speed of 0.004, 0.006, and 0.01 mm/min; fatigue crack growth tests at load ratios (R = Pmin/Pmax) of 0.1, 0.2, and 0.5; and low cycle fatigue tests at four strain amplitudes of (0.3‐0.6)% are performed for SENS50 and SENS75 alloys. Relatively lower magnitude of fracture toughness values are observed for SENS75 specimen. Severe degradation in tensile properties, fatigue crack growth characteristics, and low cycle fatigue lives are observed for SENS75 samples. Extended finite element method is adopted to simulate the elasto‐plastic crack growth during fracture toughness evaluation. Scanning electron microscopy (SEM) is used to understand the failure mechanism of sensitized alloys. 相似文献