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151.
Parastoo Memarian Elham Pishavar Federica Zanotti Martina Trentini Francesca Camponogara Elisa Soliani Paolo Gargiulo Maurizio Isola Barbara Zavan 《International journal of molecular sciences》2022,23(3)
The successful clinical application of bone tissue engineering requires customized implants based on the receiver’s bone anatomy and defect characteristics. Three-dimensional (3D) printing in small animal orthopedics has recently emerged as a valuable approach in fabricating individualized implants for receiver-specific needs. In veterinary medicine, because of the wide range of dimensions and anatomical variances, receiver-specific diagnosis and therapy are even more critical. The ability to generate 3D anatomical models and customize orthopedic instruments, implants, and scaffolds are advantages of 3D printing in small animal orthopedics. Furthermore, this technology provides veterinary medicine with a powerful tool that improves performance, precision, and cost-effectiveness. Nonetheless, the individualized 3D-printed implants have benefited several complex orthopedic procedures in small animals, including joint replacement surgeries, critical size bone defects, tibial tuberosity advancement, patellar groove replacement, limb-sparing surgeries, and other complex orthopedic procedures. The main purpose of this review is to discuss the application of 3D printing in small animal orthopedics based on already published papers as well as the techniques and materials used to fabricate 3D-printed objects. Finally, the advantages, current limitations, and future directions of 3D printing in small animal orthopedics have been addressed. 相似文献
152.
Federico Bolognesi Nicola Fazio Filippo Boriani Viscardo Paolo Fabbri Davide Gravina Francesca Alice Pedrini Nicoletta Zini Michelina Greco Michela Paolucci Maria Carla Re Sofia Asioli Maria Pia Foschini Antonietta DErrico Nicola Baldini Claudio Marchetti 《International journal of molecular sciences》2022,23(3)
Defects of the peripheral nervous system are extremely frequent in trauma and surgeries and have high socioeconomic costs. If the direct suture of a lesion is not possible, i.e., nerve gap > 2 cm, it is necessary to use grafts. While the gold standard is the autograft, it has disadvantages related to its harvesting, with an inevitable functional deficit and further morbidity. An alternative to autografting is represented by the acellular nerve allograft (ANA), which avoids disadvantages of autograft harvesting and fresh allograft rejection. In this research, the authors intend to transfer to human nerves a novel technique, previously implemented in animal models, to decellularize nerves. The new method is based on soaking the nerve tissues in decellularizing solutions while associating ultrasounds and freeze–thaw cycles. It is performed without interrupting the sterility chain, so that the new graft may not require post-production γ-ray irradiation, which is suspected to affect the structural and functional quality of tissues. The new method is rapid, safe, and inexpensive if compared with available commercial ANAs. Histology and immunohistochemistry have been adopted to evaluate the new decellularized nerves. The study shows that the new method can be applied to human nerve samples, obtaining similar, and, sometimes better, results compared with the chosen control method, the Hudson technique. 相似文献
153.
Dr. Ivan A. Yaremenko Dr. Paolo Coghi Parichat Prommana Congling Qiu Peter S. Radulov Yuanqing Qu Yulia Yu. Belyakova Dr. Enrico Zanforlin Dr. Vladimir A. Kokorekin Yuki Yu Jun Wu Prof. Fabrice Fleury Dr. Chairat Uthaipibull Dr. Vincent Kam Wai Wong Prof. Alexander O. Terent'ev 《ChemMedChem》2020,15(13):1118-1127
This article discloses a new horizon for the application of peroxides in medical chemistry. Stable cyclic peroxides are demonstrated to have cytotoxic activity against cancer cells; in addition a mechanism of cytotoxic action is proposed. Synthetic bridged 1,2,4,5-tetraoxanes and ozonides were effective against HepG2 cancer cells and some ozonides selectively targeted liver cancer cells (the selectivity indexes for compounds 11 b and 12 a are 8 and 5, respectively). In some cases, tetraoxanes and ozonides were more selective than paclitaxel, artemisinin, and artesunic acid. Annexin V flow-cytometry analysis revealed that the active ozonides 22 a and 23 a induced cell death of HepG2 by apoptosis. Further study showed that compounds 22 a and 23 a exhibited a strong inhibitory effect on P-glycoprotein (P-gp/ABCB5)-overexpressing HepG2 cancer cells. ABCB5 is a key player in the multidrug-resistant phenotype of liver cancer. Peroxides failed to demonstrate a direct correlation between oxidative potential and their biological activity. To our knowledge this is the first time that peroxide diastereoisomers have been found to show stereospecific antimalarial action against the chloroquine-sensitive 3D7 strain of Plasmodium falciparum. Stereoisomeric ozonide 12 b is 11 times more active than stereoisomeric ozonide 12 a (IC50=5.81 vs 65.18 μm ). Current findings mean that ozonides merit further investigation as potential therapeutic agents for drug-resistant hepatocellular carcinoma. 相似文献
154.
Raffaele Raimondi Piero Zollet Francesco Paolo De Rosa Panagiotis Tsoutsanis Matteo Stravalaci Marianna Paulis Antonio Inforzato Mario R. Romano 《International journal of molecular sciences》2022,23(2)
The retinal pigmented epithelium (RPE) plays a pivotal role in retinal homeostasis. It is therefore an interesting target to fill the unmet medical need of different retinal diseases, including age-related macular degeneration and Stargardt disease. RPE replacement therapy may use different cellular sources: induced pluripotent stem cells or embryonic stem cells. Cells can be transferred as suspension on a patch with different surgical approaches. Results are promising although based on very limited samples. In this review, we summarize the current progress of RPE replacement and provide a comparative assessment of different published approaches which may become standard of care in the future. 相似文献
155.
Biagio Barone Luigi Napolitano Marco Abate Luigi Cirillo Pasquale Reccia Francesco Passaro Carmine Turco Simone Morra Francesco Mastrangelo Antonio Scarpato Ugo Amicuzi Vincenzo Morgera Lorenzo Romano Francesco Paolo Calace Savio Domenico Pandolfo Luigi De Luca Achille Aveta Enrico Sicignano Massimiliano Trivellato Gianluca Spena Carlo DAlterio Giovanni Maria Fusco Raffaele Vitale Davide Arcaniolo Felice Crocetto 《International journal of molecular sciences》2022,23(7)
Testosterone is the most important hormone in male health. Aging is characterized by testosterone deficiency due to decreasing testosterone levels associated with low testicular production, genetic factors, adiposity, and illness. Low testosterone levels in men are associated with sexual dysfunction (low sexual desire, erectile dysfunction), reduced skeletal muscle mass and strength, decreased bone mineral density, increased cardiovascular risk and alterations of the glycometabolic profile. Testosterone replacement therapy (TRT) shows several therapeutic effects while maintaining a good safety profile in hypogonadal men. TRT restores normal levels of serum testosterone in men, increasing libido and energy level and producing beneficial effects on bone density, strength and muscle as well as yielding cardioprotective effects. Nevertheless, TRT could be contraindicated in men with untreated prostate cancer, although poor findings are reported in the literature. In addition, different potential side effects, such as polycythemia, cardiac events and obstructive sleep apnea, should be monitored. The aim of our review is to provide an updated background regarding the pros and cons of TRT, evaluating its role and its clinical applicability in different domains. 相似文献
156.
Elena Bresciani Nicola Squillace Valentina Orsini Roberta Piolini Laura Rizzi Laura Molteni Ramona Meanti Alessandro Soria Giuseppe Lapadula Alessandra Bandera Andrea Gori Paolo Bonfanti Robert John Omeljaniuk Vittorio Locatelli Antonio Torsello 《International journal of molecular sciences》2022,23(7)
Combined AntiRetroviral Treatments (cARTs) used for HIV infection may result in varied metabolic complications, which in some cases, may be related to patient genetic factors, particularly microRNAs. The use of monozygotic twins, differing only for HIV infection, presents a unique and powerful model for the controlled analysis of potential alterations of miRNAs regulation consequent to cART treatment. Profiling of 2578 mature miRNA in the subcutaneous (SC) adipose tissue and plasma of monozygotic twins was investigated by the GeneChip® miRNA 4.1 array. Real-time PCR and ddPCR experiments were performed in order to validate differentially expressed miRNAs. Target genes of deregulated miRNAs were predicted by the miRDB database (prediction score > 70) and enrichment analysis was carried out with g:Profiler. Processes in SC adipose tissue most greatly affected by miRNA up-regulation included (i) macromolecular metabolic processes, (ii) regulation of neurogenesis, and (iii) protein phosphorylation. Furthermore, KEGG analysis revealed miRNA up-regulation involvement in (i) insulin signaling pathways, (ii) neurotrophin signaling pathways, and (iii) pancreatic cancer. By contrast, miRNA up-regulation in plasma was involved in (i) melanoma, (ii) p53 signaling pathways, and (iii) focal adhesion. Our findings suggest a mechanism that may increase the predisposition of HIV+ patients to insulin resistance and cancer. 相似文献
157.
Martina Magni Chiara Paolizzi Chiara Monfrini Cristina Vella Paolo Corradini Cristiana Carniti 《International journal of molecular sciences》2022,23(7)
Mature T-cell lymphomas (MTCLs) represent a heterogeneous group of aggressive non-Hodgkin lymphomas comprising different entities. Anthracycline-based regimens are considered the standard of care in the front-line treatment. However, responses to these approaches have been neither adequate nor durable, and new treatment strategies are urgently needed to improve survival. Genomic instability is a common feature of cancer cells and can be caused by aberrations in the DNA damage response (DDR) and DNA repair mechanisms. Consistently, molecules involved in DDR are being targeted to successfully sensitize cancer cells to chemotherapy. Recent studies showed that some hematological malignancies display constitutive DNA damage and intrinsic DDR activation, but these features have not been investigated yet in MTCLs. In this study, we employed a panel of malignant T cell lines, and we report for the first time the characterization of intrinsic DNA damage and basal DDR activation in preclinical models in T-cell lymphoma. Moreover, we report the efficacy of targeting the apical kinase ATM using the inhibitor AZD0156, in combination with standard chemotherapy to promote apoptotic cell death. These findings suggest that DDR is an attractive pathway to be pharmacologically targeted when developing novel therapies and improving MTCL patients’ outcomes. 相似文献
158.
Francesca Antonucci Federico Pallottino Graziella Paglia Amedeo Palma Salvatore D��Aquino Paolo Menesatti 《Food and Bioprocess Technology》2011,4(5):809-813
Sugar content is one of the most important quality attributes of citrus fruit, either for fresh or for processing market.
Since sugars in citrus juice are highly correlated with total soluble solids (TSS) content, which can be determined easily
even by the means of a hand refractometer, TSS is one of the most frequently used quality index. Since TSS can be measured
only destructively, the results are representative only if carried out on large samples and do not allow classifying marketable
fruit one by one according to their specific sugar content. Objective of this experiment was to assess possibility and limits
of a non-destructive estimation of citrus fruits internal quality parameters (TSS and titratable acidity) presenting thick
peel by the use of a spectrophotometric portable VIS-NIR system. Four hundred fruit of “Miho” satsuma and 150 fruit of “Page”
tangelo were used. Each fruit was first subjected to spectrophotometric acquisition and soon after was juiced and TSS and
titratable acidity (TA) determined. Partial least squares (PLS) regression analysis was applied for constructing a predictive
model based on the spectral normalized response, constructing the model on a sub-sample and verifying the model (prediction
test) on independent ones. The TA relative to Page mandarin was predicted in the test with an r = 0.88 and a standard error of prevision (SEP) coefficient of variability of 3.8% while the TSS scored an r = 0.85 and a SEP coefficient of variability equal to 4%. The TA of Miho mandarin was predicted in the test with an r = 0.81 and a SEP coefficient of Variability of 8.3% while the TSS scored an r = 0.84 and a SEP coefficient of variability equal to 5.6%. 相似文献
159.
Supervised Multivariate Analysis of Hyper-spectral NIR Images to Evaluate the Starch Index of Apples 总被引:4,自引:0,他引:4
Paolo Menesatti Angelo Zanella Stefano D’Andrea Corrado Costa Graziella Paglia Federico Pallottino 《Food and Bioprocess Technology》2009,2(3):308-314
Fruit maturity indexes are crucial in harvest time determination and commercial context. The harvest time of apples, matching
the desired commercial characteristics, is assessed through starch–iodine test in practice. Fruit halves are dipped into iodine
solution and patterns are visually evaluated by experts comparing them to reference charts. Aim of the work was to study the
relationships of near infrared (NIR) spectral images (1,000–1,700 nm), starch/starch-free patterns visually assessed and RGB
color images. Spectral images of 88 Golden Delicious Klon B apples were sampled at seven different maturity stages. Partial
least-squares discriminant analysis (PLSDA) technique was used on hyper-spectral NIR images to classify single pixels using
its NIR reflectance spectrum. The response variable (i.e. the classification for each pixel) was identified through the matching
of single pixel obtained with the color images, segmented in two classes (starch and starch-free), and the NIR hyper-spectral
matrix. Mean hyper-spectral classification obtained through PLSDA modeling on individual apple correctly classified 80.81%
of the total pixels, while the unique model, i.e. a single model including all the fruits, resulted in 66.33%. In the latter
case, the relationship with the RGB classification showed high values (Pearson correlation coefficient r = 0.95). The present work shows the feasibility of NIR imaging spectroscopy as a tool for apple fruit maturity determination,
avoiding expert’s subjective interpretation by traditional starch index assignments. 相似文献
160.