Spice use in food: Properties and benefits

Spices are parts of plants that due to their properties are used as colorants, preservatives, or medicine. The uses of spices have been known since long time, and the interest in the potential of spices is remarkable due to the chemical compounds contained in spices, such as phenylpropanoids, terpenes, flavonoids, and anthocyanins. Spices, such as cumin (cuminaldehyde), clove (eugenol), and cinnamon (cinnamaldehyde) among others, are known and studied for their antimicrobial and antioxidant properties due to their main chemical compounds. These spices have the potential to be used as preservatives in many foods namely in processed meat to replace chemical preservatives. Main chemical compounds in spices also confer other properties providing a variety of applications to spices, such as insecticidal, medicines, colorants, and natural flavoring. Spices provide beneficial effects, such as antioxidant activity levels that are comparable to regular chemical antioxidants used so they can be used as a natural alternative to synthetic preservatives. In this review, the main characteristics of spices will be described as well as their chemical properties, different applications of these spices, and the advantages and disadvantages of their use.     

Production of human growth hormone by Lactococcus lactis

A synthetic gene coding for human growth hormone was expressed in Lactococcus lactis. The presence of the recombinant protein was assayed and quantified using ELISA tests. Human growth hormone was detected at high concentrations and displayed a biological activity similar to the one shown by commercial human growth hormone.     

Endothelial repair process and its relevance to longitudinal neointimal tissue patterns: comparing histology with in silico modelling

Re-establishing a functional endothelium following endovascular treatment is an important factor in arresting neointimal proliferation. In this study, both histology (in vivo) and computational simulations (in silico) are used to evaluate neointimal growth patterns within coronary arteries along the axial direction of the stent. Comparison of the growth configurations in vivo and in silico was undertaken to identify candidate mechanisms for endothelial repair. Stent, lumen and neointimal areas were measured from histological sections obtained from eight right coronary stented porcine arteries. Two re-endothelialization scenarios (endothelial cell (EC) random seeding and EC growth from proximal and distal ends) were implemented in silico to evaluate their influence on the morphology of the simulated lesions. Subject to the assumptions made in the current simulations, comparison between in vivo and in silico results suggests that endothelial growth does not occur from the proximal and distal ends alone, but is more consistent with the assumption of a random seeding process. This may occur either from the patches of endothelium which survive following stent implantation or from attachment of circulating endothelial progenitor cells.     

Genetic analysis of geraniol metabolism during fermentation

Geraniol produced by grape is the main precursor of terpenols which play a key role in the floral aroma of white wines. We investigated the fate of geraniol during wine fermentation by Saccharomyces cerevisiae. The volatile compounds produced during fermentation of a medium enriched with geraniol were extracted by Stir-bar sorptive extraction and analysed by GC–MS. We were able to detect and quantify geranyl acetate but also citronellyl- and neryl-acetate. The presence of these compounds partly explains the disparition of geraniol. The amounts of terpenyl esters are strain dependant. We demonstrated both by gene overexpression and gene-deletion the involvement of ATF1 enzyme but not ATF2 in the acetylation of terpenols. The affinity of ATF1 enzyme for several terpenols and for isoamyl alcohol was compared. We also demonstrated that OYE2 is the enzyme involved in geraniol to citronellol reduction. Fermenting strain deleted from OYE2 gene produces far less citronellol than wild type strain. Moreover lab strain over-expressing OYE2 allows 87% geraniol to citronellol reduction in bioconversion experiment compared to about 50% conversion with control strain.     

Using pore space 3D geometrical modelling to simulate biological activity: Impact of soil structure

This study is the follow-up to a previous one devoted to soil pore space modelling. In the previous study, we proposed algorithms to represent soil pore space by means of optimal piecewise approximation using simple 3D geometrical primitives: balls, cylinders, cones, etc. In the present study, we use the ball-based piecewise approximation to simulate biological activity. The basic idea for modelling pore space consists in representing pore space using a minimal set of maximal balls (Delaunay spheres) recovering the shape skeleton. In this representation, each ball is considered as a maximal local cavity corresponding to the “intuitive” notion of a pore as described in the literature. The space segmentation induced by the network of balls (pores) is then used to spatialise biological dynamics. Organic matter and microbial decomposers are distributed within the balls (pores). A valuated graph representing the pore network, organic matter and microorganism distribution is then defined. Microbial soil organic matter decomposition is simulated by updating this valuated graph. The method has been implemented and tested on real data. As far as we know, this approach is the first one to formally link pore space geometry and biological dynamics. The long-term goal is to define geometrical typologies of pore space shape that can be attached to specific biological dynamic properties. This paper is a first attempt to achieve this goal.     

Electrochemical behavior of multifunctional graphene–polyimide nanocomposite film in two different electrolyte solutions

The effect of solvent on specific capacitance, bulk resistance, and charge/discharge capacity of graphene/polyimide composite films is studied by electrochemical methods. Composite films are synthesized by in situ condensation polymerization of poly (amic acid) in the presence of 50 wt % partly exfoliated graphene sheets followed by thermal curing at 250°C. Raman spectrum of the exfoliated graphene sheets show an increase in the ratio of I_D to I_G peak intensities from 0.167 to 0.222, suggesting increased defects in graphene basal planes. Electrochemical measurements carried out by using 0.4M potassium hexafluorophosphate (KPF₆) dissolved in propylene carbonate and N‐methylpyrrolidone at 25°C show that the composite system exhibits both pseudocapacitance and supercapacitance behaviors, with an average capacitance of 40 and 36.5 F g^?1, respectively. Bulk resistance of the composite obtained by using KPF₆–propylene carbonate electrolyte solution is 300% lower than that obtained in KPF₆–N‐methylpyrrolidone solution, with a fairly stable specific capacity of 85 μAhr g^?1, with 80% retention observed after 30 charge–discharge cycles. Fourier transform infrared spectroscopy measurements show shifts in the cyclic imide carbonyl peak from 1778 to 1774 cm^?1, which suggests that some form of interaction exists between the graphene and polyimide. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 42673.     

The impact of childhood symptoms of conduct disorder on driver aggression in adulthood

Background

Despite limited empirical investigation, existing scientific literature suggests that individuals with a history or current diagnosis of conduct disorder (CD) may be more likely to demonstrate reckless and aggressive driving. Much of the limited research in this field examines the impact of childhood CD on driver behaviour and collision risk in young adults. Few if any, studies assess the impact of this disorder on driver behaviour beyond age 21 years. The current research is a population-based study of the impact of CD symptoms during childhood on the risk of engaging in driver aggression during adulthood.

Methods

Data are based on telephone interviews with 5230 respondents who reported having driven in the past year. Data are derived from the 2011–2013 cycles of the CAMH Monitor, an ongoing cross-sectional survey of adults in Ontario, Canada aged 18 years and older. A binary logistic regression analysis of self-reported driver aggression in the previous 12 months was conducted, consisting of measures of demographic characteristics, driving exposure, problem substance use, alcohol- and drug-impaired driving, symptoms of attention deficit hyperactivity disorder, and childhood (before age 15) symptoms of CD.

Results

When entered with demographic characteristics, driving exposure, and other potential confounders, childhood symptoms of CD increased the odds of reporting driver aggression more than two-fold (adjusted OR = 2.12). Exploratory analyses of the interaction between childhood symptoms of CD and age was not a significant predictor of driver aggression.

Conclusions

Results suggest that symptoms of CD during childhood are associated with significantly increased odds of self-reported driver aggression during adulthood. Limitations and future directions of the research are discussed.     

Conference news

RESOURCES: ENVIRONMENT AND POLICY, by John Fernie and Alan Pitkethly, Harper and Row, London, 1985, 338 pp. (#bp12.95, paperback)

THE ECONOMICS OF FORESTRY AND NATURAL RESOURCES, by Per‐Olov Johansson and Karl‐Gustaf Lofgren, Basil Blackwell, Oxford, 1985, 292 pp. (#bp29.50, hardback only)

ENVIRONMENTAL PROTECTION: STANDARDS, COMPLIANCE AND COSTS, edited by T. J. Lack, J. Wiley &; Son, Chichester, 1984, 329 pp. (#bp30, hardback)

ENVIRONMENT AND ENFORCEMENT: REGULATION AND THE SOCIAL DEFINITION OF POLLUTION, by K. Hawkins, Clarendon Press, Oxford, 1984, 253 pp. (#bp15 hardback; #bp6.95, paperback)

ENVIRONMENTAL POLICY, VOLUME 1: ELEMENTS OF ENVIRONMENTAL ANALYSIS, by George Tolley, Philip Graves and Glenn Blomquist, Ballinger, Cambridge, Mass, and Harper and Row, London, 1981. (#bp27.50, hardback)

ENVIRONMENTAL POLICY, VOLUME 4: SOLID WASTES, by George Tolley, Jospeh Havlicek and Robert Fabian, Ballinger, Cambridge, Mass. and Harper and Row, London, 1985. (#bp44.00, hardback)

World Health Organisation, ENVIRONMENTAL POLLUTION CONTROL IN RELATION TO DEVELOPMENT, World Health Organisation, Geneva, Technical Report Series, 718, 1985, 63 pp.

TRANSPORT IS FOR PEOPLE: NINTH INTERNATIONAL SYMPOSIUM ON THEORY AND PRACTICE IN TRANSPORT ECONOMICS, European Conference of Ministers of Transport, 1983, Paris (#bp15.00)

RECREATION MANAGEMENT AND PRICING, by A. G. Bovaird, M. J. Tricker and R. Stoakes, Gower Press, Aldershot, 1984, 182 pp. (#bp15, hardback)

URBAN LAND AND SHELTER FOR THE POOR, by Patrick McAuslan, Earthscan#shInternational Institute for Environment and Development, London, 1985, 145 pp.

CARING FOR THE UNEMPLOYED. Prepared for the Association of Metro‐polital Authorities by Susan Balloch, Chris Hume, Brian Jones and Peter Westland. Publishers: Bedford Square Press, London, 1985, for the National Council for Voluntary Organisations.

THE LONG‐TERM UNEMPLOYED. ACTION FOR A FORGOTTEN MILLION, by Peter Ashby (Head, NVCO Policy Analysis Unit). An NCVO Policy Discussion document, Bedford Square Press, London (1985).

ANTHROPOGENIC COMPOUNDS: Volume 3 Part C of The Handbook of Environmental Chemistry, edited by O. Hutzinger, Springer‐Verlag, Germany, 248 pp. 1986. (DM 188.00)

HEAVY METALS IN NATURAL WATERS: APPLIED MONITORING AND IMPACT ASSESSMENT, by James W. Moore and S. Ramamoorthy, Springer‐Verlag, U.S.A. and Germany, viii #pl 268 pages, index, 1984. (DM 108.00)

DESTRUCTION AND DISPOSAL OF PCBs BY THERMAL AND NON‐THERMAL METHODS, by D. G. Ackerman, L. L. Scinto, P. S. Bakshi, R. G. Delumyea, R. J. Johnson, G. Richard, A. M. Takata and E. M. Sworzyn. Noyes Data Corporation, Park Ridge, New Jersey, 1983, xiv #pl 417 pages (no index). (#dl48.00)

ELECTROSTATIC PRECIPITATOR MANUAL (Pollution Technology Review No. 91), by Jack R. McDonald and Alan H. Dean, Noyes Data Corporation, U.S.A., 1982. (#dl48.00)

PATTY'S INDUSTRIAL HYGIENE AND TOXICOLOGY, edited by Lewis J. Cralley and Lester V. Cralley, Volume 3 (2nd Edition)—Theory and Rationale of Industrial Hygiene Practice: (i) Volume 3A—The Work Environment (#bp97.20); (ii) Volume 3B—Biological Responses (#bp92.00) J. Wiley and Sons Ltd., Chichester U.K. 1985.

SAFE DRINKING WATER. THE IMPACT OF CHEMICALS ON A LIMITED RESOURCE, edited by R. G. Rice, J. Wiley and Sons, Chichester, U.K., Lewis Publishers Inc. Chelsea MI, 1985. (#bp36.00)

PLANTS FOR ARID LANDS. (Proceedings of the Kew International Conference on Economic Plants for Arid Lands held in the Jodrell Laboratory, Royal Botanic Gardens, Kew, England, 23‐27 July 1984), edited by G. E. Wickens, J. R. Goodwin and D. V. Field. George Allen and Unwin, London, 1985. (#bp30.00)

REGULATED RIVERS, edited by A. Lillehammer and S. J. Saltveit, Univer‐sitetsforlaget AS, Oslo, 1984. (#bp47.50)

NUTRIENT CYCLING IN TROPICAL FOREST ECOSYSTEMS, by C. F. Jordan, Wiley, Chichester, 1985. (#bp13.45)     

Artificial Maturation of the Highly Active Heterodimeric [FeFe] Hydrogenase from Desulfovibrio desulfuricans ATCC 7757

Hydrogenases catalyze the reduction of protons and oxidation of molecular hydrogen with high turnover frequencies and low overpotentials under ambient conditions. The heterodimeric [FeFe] hydrogenase from Desulfovibrio desulfuricans has an exceptionally high activity, and can be purified aerobically in an oxygen-stable inactive state. Recently, it was demonstrated that monomeric [FeFe] hydrogenases produced recombinantly in Escherichia coli can be artificially maturated by simply incubating the inactive “apo” enzymes with the synthetic [2Fe] cofactor mimic [Fe₂(adt)(CO)₄(CN)₂]²⁻. Here, we use the same technique to produce the heterodimeric “apo” hydrogenase from D. desulfuricans in E. coli with a high yield and purity, and maturate the “apo” enzyme with [Fe₂(adt)(CO)₄(CN)₂]²⁻ to generate fully active “holo” enzyme. Interestingly, the rate of the artificial maturation process with D. desulfuricans is significantly slower than that for all other hydrogenases tested so far. The artificially maturated enzyme is spectroscopically and electrochemically identical to the native enzyme and shows high rates of hydrogen production (3700 s⁻¹) and hydrogen oxidation (63,000 s⁻¹). We expect that our highly efficient production method will facilitate future studies of this enzyme and other related [FeFe] hydrogenases from Desulfovibrio species.     

Potential process ‘hurdles’ in the use of macroalgae as feedstock for biofuel production in the British Isles

This review examines the potential technical and energy balance hurdles in the production of seaweed biofuel, and in particular for the MacroBioCrude processing pipeline for the sustainable manufacture of liquid hydrocarbon fuels from seaweed in the UK. The production of biofuel from seaweed is economically, energetically and technically challenging at scale. Any successful process appears to require both a method of preserving the seaweed for continuous feedstock availability and a method exploiting the entire biomass. Ensiling and gasification offer a potential solution to these two requirements. However there is need for more data particularly at a commercial scale. © 2016 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.