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91.
Patricia Bondia Rocío Jurado Santiago Casado José M. Domínguez‐Vera Natividad Gálvez Cristina Flors 《Small (Weinheim an der Bergstrasse, Germany)》2017,13(17)
The combination of complementary techniques to characterize materials at the nanoscale is crucial to gain a more complete picture of their structure, a key step to design and fabricate new materials with improved properties and diverse functions. Here it is shown that correlative atomic force microscopy (AFM) and localization‐based super‐resolution microscopy is a useful tool that provides insight into the structure and emissive properties of fluorescent β‐lactoglobulin (βLG) amyloid‐like fibrils. These hybrid materials are made by functionalization of βLG with organic fluorophores and quantum dots, the latter being relevant for the production of 1D inorganic nanostructures templated by self‐assembling peptides. Simultaneous functionalization of βLG fibers by QD655 and QD525 allows for correlative AFM and two‐color super‐resolution fluorescence imaging of these hybrid materials. These experiments allow the combination of information about the topography and number of filaments that compose a fibril, as well as the emissive properties and nanoscale spatial distribution of the attached fluorophores. This study represents an important step forward in the characterization of multifunctionalized hybrid materials, a key challenge in nanoscience. 相似文献
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Thermal Scanning at the Cellular Level by an Optically Trapped Upconverting Fluorescent Particle
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Daniel Vázquez-Nion Federica Troiano Patricia Sanmartín Chiara Valagussa Francesca Cappitelli Beatriz Prieto 《Materials and Structures》2018,51(6):158
Salt crystallisation is a very common and powerful weathering agent that can modify the petrophysical properties of building stone such as granite. In addition, the weathering can affect the susceptibility of the stone to biological colonisation. The aims of the present study were to examine the properties of a granite weathered by sodium chloride crystallisation and to evaluate the effects of the weathering on the secondary bioreceptivity of the stone to subaerial phototrophic biofilms. For this purpose, granite samples were subjected to a laboratory-based accelerated salt weathering test, and changes in weight, open porosity, bulk density, capillary water content, abrasion pH and surface roughness of the samples were determined. Samples of both weathered and non-weathered granite were then inoculated with a multi-species phototrophic culture derived from a natural subaerial biofilm and incubated under standardised laboratory conditions for 3 months. The weight loss produced by the weathering process was consistent with significant changes in abrasion pH and surface roughness. The bioreceptivity of the stone was also altered. According to the bioreceptivity index, the granite under study was characterised by ‘mild primary bioreceptivity’, but ‘high secondary bioreceptivity’ after the salt weathering process. Study of the secondary bioreceptivity of stone materials can provide very useful information about response to weathering effects, and the findings can be used to improve the selection of materials for building purposes. 相似文献
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Mónica Giménez‐Marqués Elena Bellido Thomas Berthelot Teresa Simón‐Yarza Tania Hidalgo Rosana Simón‐Vázquez África González‐Fernández José Avila Maria Carmen Asensio Ruxandra Gref Patrick Couvreur Christian Serre Patricia Horcajada 《Small (Weinheim an der Bergstrasse, Germany)》2018,14(40)
Controlling the outer surface of nanometric metal–organic frameworks (nanoMOFs) and further understanding the in vivo effect of the coated material are crucial for the convenient biomedical applications of MOFs. However, in most studies, the surface modification protocol is often associated with significant toxicity and/or lack of selectivity. As an alternative, how the highly selective and general grafting GraftFast method leads, through a green and simple process, to the successful attachment of multifunctional biopolymers (polyethylene glycol (PEG) and hyaluronic acid) on the external surface of nanoMOFs is reported. In particular, effectively PEGylated iron trimesate MIL‐100(Fe) nanoparticles (NPs) exhibit suitable grafting stability and superior chemical and colloidal stability in different biofluids, while conserving full porosity and allowing the adsorption of bioactive molecules (cosmetic and antitumor agents). Furthermore, the nature of the MOF–PEG interaction is deeply investigated using high‐resolution soft X‐ray spectroscopy. Finally, a cell penetration study using the radio‐labeled antitumor agent gemcitabine monophosphate (3H‐GMP)‐loaded MIL‐100(Fe)@PEG NPs shows reduced macrophage phagocytosis, confirming a significant in vitro PEG furtiveness. 相似文献
98.
D. Kennedy D. Miller Victor QuintanaAuthor vitae 《International Journal of Electrical Power & Energy Systems》1998,20(8):501-515
In this paper, a unified approach to the design of a nonlinear excitation controller/power system stabilizer for a synchronous generator/infinite bus power system is presented. The approach is based on a form of state feedback linearization, known as input–output feedback linearization, which provides an exact semi-global state transformation that is valid for a large class of operating points of the power system. With this transformation, the terminal voltage becomes a linear function of the control input. The excitation controller/power system stabilizer is then synthesized by using linear controller design techniques. The controller is proven to provide small signal stability and to provide local asymptotic tracking of admissible constant reference signals for a large class of operating points. A procedure is given to tune the controller gains to provide significant damping of the power angle oscillations. 相似文献
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Pille Mänd Lea Hallik Josep Peñuelas Pierpaolo Duce Claus Beier János Garadnai Inger Kappel Schmidt Patricia Prieto Joke W. Westerveld 《Remote sensing of environment》2010,114(3):626-297
The aim of this study was to evaluate the use of ground-based canopy reflectance measurements to detect changes in physiology and structure of vegetation in response to experimental warming and drought treatment at six European shrublands located along a North-South climatic gradient. We measured canopy reflectance, effective green leaf area index (green LAIe) and chlorophyll fluorescence of dominant species. The treatment effects on green LAIe varied among sites. We calculated three reflectance indices: photochemical reflectance index PRI [531 nm; 570 nm], normalized difference vegetation index NDVI680 [780 nm; 680 nm] using red spectral region, and NDVI570 [780 nm; 570 nm] using the same green spectral region as PRI. All three reflectance indices were significantly related to green LAIe and were able to detect changes in shrubland vegetation among treatments. In general warming treatment increased PRI and drought treatment reduced NDVI values. The significant treatment effect on photochemical efficiency of plants detected with PRI could not be detected by fluorescence measurements. However, we found canopy level measured PRI to be very sensitive to soil reflectance properties especially in vegetation areas with low green LAIe. As both soil reflectance and LAI varied between northern and southern sites it is problematic to draw universal conclusions of climate-derived changes in all vegetation types based merely on PRI measurements. We propose that canopy level PRI measurements can be more useful in areas of dense vegetation and dark soils. 相似文献
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