REMOVAL OF IRON FROM KAOLIN ORES USING DIFFERENT MICROORGANISMS. THE ROLE OF THE ORGANIC ACIDS AND FERRIC IRON REDUCTASE

In the present work the removal of iron, present as impurity in a kaolin of industrial interest, was performed. An effective iron reduction using microbial mixed cultures was attained (up to 81%) while a significant iron reduction in the presence of single microbial cultures was achieved (up to 37%).

The active microorganisms belong to the genera Bacillus and Agrobacter. The enzymatic and un-enzymatic iron reduction was evaluated.

Finally the molasses as carbon source for heterotrophic bacteria were succesfully tested.     

Treatment of gasoline-contaminated waters by advanced oxidation processes

In this study, the efficiency of advanced oxidative processes (AOPs) was investigated toward the degradation of aqueous solutions containing benzene, toluene and xylenes (BTX) and gasoline-contaminated waters. The results indicated that BTX can be effectively oxidized by near UV-assisted photo-Fenton process. The treatment permits almost total degradation of BTX and removal of more than 80% of the phenolic intermediates at reaction times of about 30 min. Preliminary investigations using water contaminated by gasoline suggest a good potentiality of the process for the treatment of large volumes of aqueous samples containing these polluting species. Heterogeneous photocatalysis and H2O2/UV system show lower degradation efficiency, probably due to the heterogeneous character of the TiO2-mediated system and lost of photonic efficiency of the H2O2/UV system in the presence of highly colored intermediated.     

Effect of the diuretic furosemide on urinary essential nutrient loss and on body stores in growing rats

Studies using a model of non-infectious diarrhea, have shown that increasing fecal mass by using laxatives resulted in greater fecal losses of nutrients and lower intestinal absorption. In the present study we used a diuretic to determine if increasing urine volume could result in greater urinary losses of essential nutrients. This is a relevant question because diuretics are widely and successfully used in the treatment of diseases associated with water retention and hypertension. They are known to increase potassium losses. However, there is less information on the effect of diuretics on the urinary losses of essential nutrients. Accordingly, urinary nitrogen, phosphorous, sodium, potassium, magnesium, zinc and retinol were measured in young rats consuming increasing concentrations of furosemide (0, 0.5, 1.0, 1.5 mg/g diet) in the diet over 15 days. The results showed that dietary furosemide caused a dose-dependent polyuria. In addition it reduced food intake and feed efficiency and leaded to poor growth and greater urinary losses of all the measured nutrients and electrolytes. These losses were proportional to urine volume and represented an important fraction of the rats daily intake. The losses were negatively associated with the body and liver content of the same electrolytes and nutrients. In general, this study showed that the diuretic furosemide caused malnutrition in a short period of time by reducing food intake as well as the capacity of retaining macro and micronutrients including the liposoluble vitamin A in a relatively short period of time. This study, together with our previous studies on diarrhea, indicate that proper nutrient utilization requires both an adequate intestinal and renal function.     

Transforming Unbalanced Multiway trees into a practical external data structure

Summary Unbalanced Multiway Trees (UM-trees) of degree m are external data structures wherein each node may be linked to at most m subtrees. Although they allow fast searches and updates UM-trees show poor space efficiency. In order to improve their time and space performance an overflow technique similar to that used previously for B ^*-trees is adapted to UM-trees. Analytical results show that when compared with UM-trees the new data structure improves average searching time and substantially increases space efficiency. Asymptotic space efficiency, measured as the ratio of space used to space generated, is 50% in the worst case and in the average case it is upper bounded by at least 85.7% for any m. Simulations suggest that this upper bound is tight. Compared with a homologous variant of B ^*-trees, simulation results indicate that the data structure proposed is highly balanced with comparable average space efficiency and lower average searching time. We conclude that overflow techniques can be applied not only to bottom-up type trees (e.g., B-trees) but also to top-down type trees such as UM-trees, in cases when the performance of an external data structure needs to be improved with little overhead.     

Evaluation of nutritional status by immunologic assessment in bulimia nervosa: influence of body mass index and vomiting episodes

The nutritional status of 21 patients suffering from bulimia nervosa was evaluated by anthropometric and immunologic indexes in comparison with a control group (n = 15). In addition, the influence of body mass index (BMI; in kg/m2) values and vomiting episodes on the nutritional status of bulimic patients was assessed. Anthropometry showed no signs of malnutrition in either group, except for those patients with low weights (BMI < 19). Bulimic patients had lower lymphocyte counts than did control subjects, except for those without vomiting (NVBN). All T lymphocyte subsets tested as well as CD57 cells were lower (22% and 55%, respectively) in bulimic patients than in control subjects, but the CD19 cell subset remained unmodified. The low-weight bulimic group (LWBN) had lower CD4 cell counts than did the normal-weight (BMI > 19) bulimic group. The NVBN group had lymphocyte subpopulations similar to those in the control group, except for CD57, which was lower. The bulimic patients with vomiting had the lowest cell subset values. These results suggest a depleted nutritional status in all bulimic patients studied, even those with normal weights. The LWBN group had the most depleted nutritional status and the NVBN group was least affected at a subclinical level. CD57 can be considered a good marker of nutritional status in this syndrome because it was the only subpopulation altered in all groups.     

Polymer‐based catalysts for toluene hydrogenation

Crosslinked poly(4‐vinylpyridine‐co‐styrene) was synthesized by radical polymerization. Catalysts having 1 wt % Pd were obtained by impregnation of a copolymer, poly(4‐vinylpyridine‐co‐styrene) with a Pd colloidal dispersion. We modified metal particle sizes by changing the aging period of the colloidal dispersion, with the average size in the range of 2.5–4.3 nm. The most probable structure of the metal cluster attached to the polymers is described. X‐ray diffraction, transmission electron microscopy (TEM), and H₂? O₂ titrations were used as characterization techniques. The H₂ consumption during titration was extremely low, and the calculated metal dispersion was between 15 and 25 times lower than those estimated from TEM. This suggests that the Pd crystals were almost completely covered by the polymer. The vapor‐phase hydrogenation of toluene on resins supported Pd catalysts were studied. The catalysts in the hydrogenation of toluene exhibited low activity, and the obtention of significant selectivities to partial hydrogenation products (close to 60 mol %) was remarkable. The results are explained in terms of a significant decrease in the hydrogenation capacity due to the coverage of metal particles by the resin. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 86: 381–385, 2002     

External barium block of Shaker potassium channels: evidence for two binding sites

External barium ions inhibit K+ currents of Xenopus oocytes expressing ShH4 delta 6-46, the non-inactivating deletion of the Shaker K+ channel. At the macroscopic level, Ba2+ block comprises both a fast and a slow component. The fast component is less sensitive to Ba2+ (apparent dissociation constant at 0 mV, K(0), approximately 19.1 mM) than the slow component and is also less voltage dependent (apparent electrical distance, delta, approximately 0.14). The slow component (K(0), approximately 9.4 mM, delta approximately 0.25) is relieved by outward K+ current, which suggests that the corresponding binding site resides within the channel conduction pathway. At the single channel level, the fast component of block is evidenced as an apparent reduction in amplitude, suggesting an extremely rapid blocking and unblocking reaction. In contrast, the slow component appears to be associated with long blocked times that are present from the beginning of a depolarizing command. Installation of the slow component is much slower than a diffusion limited process; for example, the blocking time constant (tau) produced by 2 mM Ba2+ is approximately 159 s (holding potential, HP = -90 mV). However, the blocking rate of this slow component is not a linear function of external Ba2+ and tends to saturate at higher concentrations. This is inconsistent with a simple bi-molecular blocking reaction. These features of external Ba2+ block can be accounted for by a simple model of two sequential Ba2+ binding sites, where the deeper of the two sites produces the slow component of block.     

Gravity and elevation changes in the Travale geothermal field (Tuscany) Italy

The results of precise levelling measurements on a specially constructed network of benchmarks in the Travale geothermal area (Tuscany, Italy) revealed the subsidence of the central part of this area, at an average rate of 20 mm/year in the period 1978 – 1980. Two sets of gravity measurements over the same time-interval, using two Lacoste — Romberg gravimeters, have an average standard error of 2–4 μGal for the main network, and 4–8 μGal for the auxiliary network. The observed g variations fall within the error range in most of the stations. The variations noted in the stations in the south-western area of the field clearly fall outside the confidence interval, and cannot entirely be attributed to changes in elevation.An absolute gravity station was set up at Palazzo at Piano (Siena), where measurements were made by the IMGC absolute gravimeter, to detect any long-term gravity variations induced by geodynamic events.     

New Methodology to Evaluate Flow Rates in On-Demand Irrigation Networks

Although Clément methodology is the most commonly used model for obtaining the design flow rate in on-demand irrigation networks, studies have shown that the Clément methodology does not always fit properly. A new stochastic methodology is proposed in this paper [random daily demand curve (RDDC)], in order to achieve a more accurate design flow. Results from Clément and the proposed RDDC methodology are compared to measured flow data in an on-demand irrigation network located in Tarazona de La Mancha (Albacete, Spain). RDDC is shown to have a better fit with the measured data compared to the Clément methodology, which underestimated the design flow by 35%–40%. RDDC methodology avoids the problem of using average opening hydrant probability, resulting in a better estimation of the network behavior.