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81.
82.
The effect of astaxanthin on the aging rat brain: gender‐related differences in modulating inflammation
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83.
Fabio Forghieri Giovanni Riva Ivana Lagreca Patrizia Barozzi Francesca Bettelli Ambra Paolini Vincenzo Nasillo Beatrice Lusenti Valeria Pioli Davide Giusti Andrea Gilioli Corrado Colasante Laura Galassi Hillary Catellani Francesca Donatelli Annalisa Talami Rossana Maffei Silvia Martinelli Leonardo Potenza Roberto Marasca Enrico Tagliafico Rossella Manfredini Tommaso Trenti Patrizia Comoli Mario Luppi 《International journal of molecular sciences》2021,22(17)
The C-terminal aminoacidic sequence from NPM1-mutated protein, absent in normal human tissues, may serve as a leukemia-specific antigen and can be considered an ideal target for NPM1-mutated acute myeloid leukemia (AML) immunotherapy. Different in silico instruments and in vitro/ex vivo immunological platforms have identified the most immunogenic epitopes from NPM1-mutated protein. Spontaneous development of endogenous NPM1-mutated-specific cytotoxic T cells has been observed in patients, potentially contributing to remission maintenance and prolonged survival. Genetically engineered T cells, namely CAR-T or TCR-transduced T cells, directed against NPM1-mutated peptides bound to HLA could prospectively represent a promising therapeutic approach. Although either adoptive or vaccine-based immunotherapies are unlikely to be highly effective in patients with full-blown leukemia, these strategies, potentially in combination with immune-checkpoint inhibitors, could be promising in maintaining remission or preemptively eradicating persistent measurable residual disease, mainly in patients ineligible for allogeneic hematopoietic stem cell transplant (HSCT). Alternatively, neoantigen-specific donor lymphocyte infusion derived from healthy donors and targeting NPM1-mutated protein to selectively elicit graft-versus-leukemia effect may represent an attractive option in subjects experiencing post-HSCT relapse. Future studies are warranted to further investigate dynamics of NPM1-mutated-specific immunity and explore whether novel individualized immunotherapies may have potential clinical utility in NPM1-mutated AML patients. 相似文献
84.
Daniela Lisini Sara Lettieri Sara Nava Giulia Accordino Simona Frigerio Chandra Bortolotto Andrea Lancia Andrea Riccardo Filippi Francesco Agustoni Laura Pandolfi Davide Piloni Patrizia Comoli Angelo Guido Corsico Giulia Maria Stella 《International journal of molecular sciences》2021,22(16)
Malignant Pleural Mesothelioma (MPM) is a rare and aggressive neoplasm of the pleural mesothelium, mainly associated with asbestos exposure and still lacking effective therapies. Modern targeted biological strategies that have revolutionized the therapy of other solid tumors have not had success so far in the MPM. Combination immunotherapy might achieve better results over chemotherapy alone, but there is still a need for more effective therapeutic approaches. Based on the peculiar disease features of MPM, several strategies for local therapeutic delivery have been developed over the past years. The common rationale of these approaches is: (i) to reduce the risk of drug inactivation before reaching the target tumor cells; (ii) to increase the concentration of active drugs in the tumor micro-environment and their bioavailability; (iii) to reduce toxic effects on normal, non-transformed cells, because of much lower drug doses than those used for systemic chemotherapy. The complex interactions between drugs and the local immune-inflammatory micro-environment modulate the subsequent clinical response. In this perspective, the main interest is currently addressed to the development of local drug delivery platforms, both cell therapy and engineered nanotools. We here propose a review aimed at deep investigation of the biologic effects of the current local therapies for MPM, including cell therapies, and the mechanisms of interaction with the tumor micro-environment. 相似文献
85.
Carletti P Masi A Spolaore B Polverino De Laureto P De Zorzi M Turetta L Ferretti M Nardi S 《Journal of chemical ecology》2008,34(6):804-818
Humic substances are known to affect plant metabolism at different levels. We characterized humic substances extracted from earthworm feces by diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy and used them to treat corn, Zea mays L., seedlings to investigate changes in patterns of root protein expression. After root plasma membrane extraction and purification, proteins were separated by two-dimensional gel electrophoresis, and differential spot intensities were evaluated by image analysis. Finally, 42 differentially expressed proteins were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The majority of them were downregulated by the treatment with humic substances. The proteins identified included malate dehydrogenase, ATPases, cytoskeleton proteins, and different enzymes belonging to the glycolytic/gluconeogenic pathways and sucrose metabolism. The identification of factors involved in plant responses to humic substances may improve our understanding of plant-soil cross-talk, and enable a better management of soil resources. 相似文献
86.
Cecchini P De Franceschi G Frare E Fontana A Polverino de Laureto P 《Protein engineering, design & selection : PEDS》2012,25(4):199-203
In order to understand the role of tryptophan in the mechanisms of fibrils formation, the ability of a series of analogs of the residue 7-18 span of myoglobin to form amyloid-like fibrils was investigated. Alternatively one or both tryptophans were substituted with alanine and leucine, to determine the contribution of hydrophobicity and aromaticity. The scale of aggregation propensity of the peptides determined indicates that tryptophan is crucial for the amyloidogenic process. Since the rare tryptophan residue is generally engaged in structural roles in proteins, or when exposed serves as binding sites, we surmise that its exposure in the amyloidogenic fragments allows for intermolecular clustering with residues from other molecules leading to the formation of amyloid aggregates. 相似文献
87.
Pfannkuchen M Godrijan J Pfannkuchen DM Iveša L Kružić P Ciminiello P Dell'Aversano C Dello Iacovo E Fattorusso E Forino M Tartaglione L Godrijan M 《Environmental science & technology》2012,46(10):5574-5582
Mass appearances of the toxic dinoflagellate genus Ostreopsis are known to cause dangerous respiratory symptoms in humans exposed to aerosols. The outbreaks can appear in shallow marine waters of temperate regions around the globe. We followed a massive bloom event on a public beach on the northern Adriatic coast near Rovinj, Croatia. We identified the responsible species and the produced toxins as well as the dynamics of the event with respect to environmental conditions. Ostreopsis cf. ovata appeared in masses from September through October 2010 on a public beach near Rovinj, Croatia but stayed undetected by public health organizations. Respiratory symptoms were observed whenever humans were exposed to substrate samples containing large numbers of Ostreopsis cells. During the mass abundance of O. cf. ovata also exposure to the aerosols on the beach evoked respiratory symptoms in humans. Our measurements showed high cell abundances and high toxin contents with a stable relative contribution of putative Palytoxin and Ovatoxins a-e. Artificial beach structures proved to dramatically reduce settling of the observed Ostreopsis biofilm. Blooms like those reported herein have a high potential to happen undetected with a high potential of affecting the health of coastal human populations. Increased monitoring efforts are therefore required to understand the ecology and toxicology of those bloom events and reduce their negative impact on coastal populations. 相似文献
88.
Patrizia Restani Francesca Uberti Chiara Tarantino Cinzia Ballabio Francesca Gombac Erica Bastiani Laura Bolognini Francesco Pavanello Roberta Danzi 《Food Analytical Methods》2012,5(3):480-486
The clarification or fining of wine removes undesirable substances such as proteins, phenols, and tannin compounds that would
cloud the wine and cause bitterness and astringency. Caseinates have useful fining properties, but their residues could present
a risk for allergic subjects. A commercial kit that was developed to detect caseinates in food has been examined for its applicability
to a wine matrix; it is sensitive to caseinates at concentrations as low as 1 ppm. The general characteristics of the caseinate
assay, described below in detail, are as follows. It is a sandwich enzyme-linked immunosorbent assay (ELISA): the microplate
is first coated with a specific anti-casein antibody; and after incubation with the wine sample, a secondary anti-casein antibody
conjugated with horseradish peroxidase is added to form a sandwich. The antibodies have been tested for their immunoreactivity
and the reproducibility of antigen recognition has been measured. An interlaboratory collaborative trial was organized to
evaluate the performance of this ELISA method and its ease of use by laboratories routinely dealing with wine/food analyses.
The results satisfy the criteria established by the International Organisation of Vine and Wine in the Compendium of International
Methods of Analysis MA-EAS1-07-ETCOL. 相似文献
89.
Phenotypic and genotypic characterization of Oenococcus oeni strains isolated from Italian wines 总被引:3,自引:0,他引:3
Guerrini S Bastianini A Blaiotta G Granchi L Moschetti G Coppola S Romano P Vincenzini M 《International journal of food microbiology》2003,83(1):1-14
A phenotypic and genotypic characterization of 84 Oenococcus oeni isolates from Italian wines of different oenological areas was carried out. Numerical analysis of fatty acid profiles grouped the isolates into two clusters at low level of similarity (63%), the minor cluster containing seven isolates besides the type and the reference strains. Forthy-eight O. oeni isolates, representative of the two clusters, showed no differences in their metabolic properties (heterolactic fermentation pattern, citrate degradation capability and formation of some secondary metabolites). Moreover, the analysis of species-specific randomly amplified polymorphic DNA and 16S-23S rDNA intergenic spacer region polymorphism as well as the sequence-specific separation of V3 region from 16S rDNA by denaturing gradient gel electrophoresis demonstrated a substantial homogeneity among the isolates. On the basis of ApaI Pulsed Field Gel Electrophoresis (PFGE) restriction patterns, the 84 isolates were grouped into five different clusters at 70% similarity, but no correlation with the phenotypic groups could be demonstrated. However, by combining phenotypic and genotypic data, the 84 O. oeni isolates grouped into eight phenotypic-genotypic combined profiles and a relationship between the origin of the isolates and their combined profile became evident, so that a sort of strain specificity can be envisaged for each wine-producing area. 相似文献
90.
Giorgina B. Piccoli Francesca Bechis Candida Iacuzzo Patrizia Anania Anna M. Iadarola Elisabetta Mezza Margherita Vischi Massimo Gai Barbara Martino Yara Garofletti Alberto Jeantet Giuseppe P. Segoloni 《Hemodialysis international. International Symposium on Home Hemodialysis》2000,4(1):47-50
The option of daily hemodialysis (HD) was discussed in November 1998 with a group of 35 HD patients on home or self‐care/limited‐care HD in a single, freestanding unit. After the meeting, 3 patients on home HD chose to switch to daily HD. The clinical success of the first patient and the immediate followers was one of the main reasons for further extension of this experience. At the time of this writing (February 2000), 10 patients were on a daily HD program (8 at home and 2 in a self‐care/limited‐care center) and one was in training for home daily HD. One further patient who tried 1 month of daily HD dropped out for logistic reasons. On daily HD, patients are dialyzed 2 – 3 hours/day, 6 days/ week, with blood flow of 270 – 300 mL/min, on bicarbonate dialysate with individually determined levels of Na and K. The schedule is flexible and a switch to 3 – 4 dialyses/week is occasionally allowed for working needs or for vacation. In addition to the well‐known clinical advantages (better well‐being, blood pressure control, nutrition, etc.), some patients preferred daily HD because of easier organization of daily activities, including work schedule. Patients initially feared frequent needle punctures and excessive burden on partners, but those concerns proved to be less a problem than anticipated. All current patients are willing to continue daily HD; only a nursing shortage limits further extension of the program in the self‐care/limited‐care center. 相似文献