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131.
Few studies in counseling and psychotherapy have investigated rater bias. The purpose of this study was to outline a method for studying rater bias. We studied three potential sources of rater bias: (a) characteristics of the rater, client, and therapist; (b) the similarity of characteristics between rater and therapist or client; and (c) perceived similarity between rater and therapist or client. We used a new rater-bias measure. The data for the study were ratings on the Collaborative Study Psychotherapy Rating Scale for 826 sessions of psychotherapy in the Treatment of Depression Collaborative Research Program. High interrater reliability was found for all scales of the measure. We found evidence of rater bias only on the facilitative conditions scale. Rater bias was not found for the other scales, perhaps because of the extensive development of the measure, careful selection of the raters, lengthy rater training, and continued contact with raters throughout the rating period. The rater-bias measure may be useful to other researchers as a means of testing the reactivity of their measures to rater bias. Finally, the method for investigating rater bias can be used by other researchers to evaluate rater bias. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
132.
Ezekiel A Chinyio Paul O Olomolaiye Pauline CorbettAuthor vitae 《International Journal of Project Management》1998,16(6):385-391
The investigation reported established a checklist of 50 different needs from which a client's specific requirements can be identified. The checklist comprises of 34 aspects of needs compiled from literature, and 16 others ascertained from an empirical investigation, through a questionnaire survey of 42 UK building clients. An evaluation of these needs indicated that `functionality of buildings', `timeliness of completion' and `value for money' are the aspects of needs, which at the moment are most frequently desired by clients. In quest of achieving optimal client satisfaction with building schemes, the generated checklist can be developed into a databank which, can easily be used for verifying a client's project requirements. 相似文献
133.
Four experiments that assessed the contributions of each side of the hypothalamus to the control of sexual behavior found the following: (1) Exposing the left, but not the right, ventromedial nucleus to estrogen neonatally defeminized sexual behavior in female rats. This asymmetry did not reverse as sexual differentiation progressed. (2) Unilateral cuts lateral to the medial preoptic area disrupted mounting in females that had mounted regularly before surgery, when given testosterone. The deficits were greater when the cuts were on the left side, but a third of the females with unilateral cuts showed severe deficits regardless of the side. (3) Comparable cuts did not impair masculine sexual behavior in gonadally intact males. In fact, left-side cuts seemed to accelerate copulation in males. (4) Unilateral lesions of the ventromedial nucleus disrupted lordosis in female rats in an essentially all-or-none fashion. This effect did not vary with side. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
134.
135.
Verhaegen PD Marle JV Kuehne A Schouten HJ Gaffney EA Maini PK Middelkoop E Zuijlen PP 《Journal of microscopy》2012,245(1):82-89
Histopathological evaluations of fibrotic processes require the characterization of collagen morphology in terms of geometrical features such as bundle orientation thickness and spacing. However, there are currently no reliable and valid techniques of measuring bundle thickness and spacing. Hence, two objective methods quantifying the collagen bundle thickness and spacing were tested for their reliability and validity: Fourier first-order maximum analysis and Distance Mapping, with the latter constituting a newly developed morphometric technique. Histological slides were constructed and imaged from 50 scar and 50 healthy human skin biopsies and subsequently analyzed by two observers to determine the interobserver reliability via the intraclass correlation coefficient. An intraclass correlation coefficient larger than 0.7 is considered as representing good reliability. The interobserver reliability for the Fourier first-order maximum and for the Distance Mapping algorithms, respectively, showed an intraclass correlation coefficient above 0.72 and 0.89. Additionally, we performed an assessment of validity in the form of responsiveness, in particular, demonstrating medium to excellent results via a calculation of the effect size, highlighting that both methods are sensitive enough to measure a treatment effect in clinical practice. In summary, two reliable and valid measurement methods were demonstrated for collagen bundle morphometry for the first time. Due to its superior reliability and more useful measures (bundle thickness and bundle spacing), Distance Mapping emerges as the preferred and more practical method. Nevertheless, in the future, both methods can be used for reliable and valid collagen morphometry of skin and scars, whereas further applications evaluating the quantitative microscopy of other fibrotic processes are anticipated. 相似文献
136.
Gabriel Ohlsson Pauline Axelsson Joshua Henry Sarunas Petronis Sofia Svedhem Bengt Kasemo 《Microfluidics and nanofluidics》2010,9(4-5):705-716
A miniaturized flow chamber for quartz crystal microbalance with dissipation monitoring (QCM-D) has been developed. The main purpose was to reduce the total liquid sample consumption during an experiment, but also to gain advantages with respect to kinetics and mass transport by reducing the boundary diffusion layer. The bottom of the flow chamber is a QCM-D sensor surface, on which a polydimethylsiloxane spacer ring, fabricated onto a poly(methyl methacrylate) lid, is placed symmetrically around the QCM-D electrode (diameter ~10 mm). The spacer ring defines the inner chamber height (typically 40–50 μm) and provides sealing. Through the lid, there are inlet and outlet channels. The typical chamber volume is in the range of 2.5–3.5 μl (with a 10 μl dead volume). In flow mode, we have operated the cell at flow rates of 6–50 μl/min, i.e., volume turnovers of 2–17 per min. As a model system, to test the microcell, the formation of supported phospholipid bilayers on a SiO2 surface was studied. For comparison, the same process was studied in a commercially available QCM-D equipment with significantly larger total volume (by a factor of 20). The decrease in effective sample consumption to produce a bilayer on the sensor surface in the chamber was approximately proportional to the decrease in chamber volume. Smaller volume also reduced the liquid exchange time. Potential improvements of the chamber include further optimization of the flow profile and, in addition, further miniaturization by decreasing the chamber height and the sensor radius. 相似文献
137.
138.
Coaggregation of κ‐Casein and β‐Lactoglobulin Produces Morphologically Distinct Amyloid Fibrils
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Jared K. Raynes Li Day Pauline Crepin John A. Carver 《Small (Weinheim an der Bergstrasse, Germany)》2017,13(14)
The unfolding, misfolding, and aggregation of proteins lead to a variety of structural species. One form is the amyloid fibril, a highly aligned, stable, nanofibrillar structure composed of β‐sheets running perpendicular to the fibril axis. β‐Lactoglobulin (β‐Lg) and κ‐casein (κ‐CN) are two milk proteins that not only individually form amyloid fibrillar aggregates, but can also coaggregate under environmental stress conditions such as elevated temperature. The aggregation between β‐Lg and κ‐CN is proposed to proceed via disulfide bond formation leading to amorphous aggregates, although the exact mechanism is not known. Herein, using a range of biophysical techniques, it is shown that β‐Lg and κ‐CN coaggregate to form morphologically distinct co‐amyloid fibrillar structures, a phenomenon previously limited to protein isoforms from different species or different peptide sequences from an individual protein. A new mechanism of aggregation is proposed whereby β‐Lg and κ‐CN not only form disulfide‐linked aggregates, but also amyloid fibrillar coaggregates. The coaggregation of two structurally unrelated proteins into cofibrils suggests that the mechanism can be a generic feature of protein aggregation as long as the prerequisites for sequence similarity are met. 相似文献
139.
Anal sac fluid from the red fox (Vulpes vulpes) was cultured for anaerobic bacteria by two techniques. The more strictly anaerobic technique yielded a wider range of species. Considerable biochemical differences were noted within named species, but no correlation could be found between the foxes and the organisms of their anal sac fluids. 相似文献
140.
Today’s reconfigurable technology provides vast parallelism that may be exploited in the design of a cellular computing machine
(CCM). In this work a virtual Sblock FPGA is implemented on an existing FPGA, achieving not only an architecture in keeping
with cellular computing principles but also suited to biologically inspired design methods. The design method proposed is
a combination of evolution and development and results of running a developmental model on the CCM are presented. 相似文献