Electron microscopy, X-ray diffraction, and atom probe tomography have been used to identify the changes which occur during the tempering of a carbide-free bainitic steel transformed at 473 K (200 °C). Partitioning of solute between ferrite and thin-films of retained austenite was observed on tempering at 673 K (400 °C) for 30 minutes. After tempering at 673 K (400 °C) and 773 K (500 °C) for 30 minutes, cementite was observed in the form of nanometre scale precipitates. Proximity histograms showed that the partitioning of solutes other than silicon from the cementite was slight at 673 K (400 °C) and more obvious at 773 K (500 °C). In both cases, the nanometre scale carbides are greatly depleted in silicon.
Ischemia reperfusion injury is common in transplantation. Previous studies have shown that cooling can protect against hypoxic injury. To date, the protective effects of hypothermia have been largely associated with metabolic suppression. Since kidney transplantation is one of the most common organ transplant surgeries, we used human-derived renal proximal tubular cells (HKC8 cell line) as a model of normal renal cells. We performed a temperature titration curve from 37 °C to 22 °C and evaluated cellular respiration and molecular mechanisms that can counteract the build-up of reducing equivalents in hypoxic conditions. We show that the protective effects of hypothermia are likely to stem both from metabolic suppression (inhibitory component) and augmentation of stress tolerance (activating component), with the highest overlap between activating and suppressing mechanisms emerging in the window of mild hypothermia (32 °C). Hypothermia decreased hypoxia-induced rise in the extracellular lactate:pyruvate ratio, increased ATP/ADP ratio and mitochondrial content, normalized lipid content, and improved the recovery of respiration after anoxia. Importantly, it was observed that in contrast to mild hypothermia, moderate and deep hypothermia interfere with HIF1 (hypoxia inducible factor 1)-dependent HRE (hypoxia response element) induction in hypoxia. This work also demonstrates that hypothermia alleviates reductive stress, a conceptually novel and largely overlooked phenomenon at the root of ischemia reperfusion injury. 相似文献
We present a new combined approach to primary bilateral cleft lip, nose, and alveolus repair using presurgical nasoalveolar molding combined with a one-stage lip, nose, and alveolus repair. Presurgical alveolar molding is used to bring the protruding premaxilla back into proper alignment with the lateral segments in the maxillary arch. Presurgical nasal molding produces tissue expansion of the short columella and nasal lining. A coordinated surgical approach involves a one-stage repair of the lip, nose, and alveolus. The nasal repair uses a retrograde approach in which the prolabial flap and columella are reflected over the nasal dorsum by continuing the dissection behind the prolabium up the membranous septum and over the septal angle. Tissues are dissected out from between the tip cartilages, and the domes are sutured together in the midline. This method joins a new class of bilateral cleft repairs that place the primary emphasis on correction of the deformity of the nasal tip cartilages. 相似文献
This paper presents a study on the coupling between a fluid solver and a motion solver to perform fluid–structure interaction (FSI) simulations of floating bodies such as point absorber wave energy converters heaving under wave loading. The two-phase fluid solver with dynamic mesh handling, interDyMFoam, is a part of the Computational Fluid Dynamics (CFD) toolbox OpenFOAM. The incompressible Navier–Stokes (NS) equations are solved together with a conservation equation for the Volume of Fluid (VoF). The motion solver is computing the kinematic body motion induced by the fluid flow. A coupling algorithm is needed between the fluid solver and the motion solver to obtain a converged solution between the hydrodynamic flow field around and the kinematic motion of the body during each time step in the transient simulation. For body geometries with a significant added mass effect, simple coupling algorithms show slow convergence or even instabilities. In this paper, we identify the mechanism for the numerical instability and we derive an accelerated coupling algorithm (based on a Jacobian) to enhance the convergence speed between the fluid and motion solver. Secondly, we illustrate the coupling algorithm by presenting a free decay test of a heaving wave energy converter. Thirdly and most challenging, a water impact test of a free falling wedge with a significant added mass effect is successfully simulated. For both test cases, the numerical results obtained by using the accelerated coupling algorithm are in a very good agreement with the experimental measurements. 相似文献
Hybrid materials displaying multistage security behavior, where a single material shows both wavelength‐ and temperature‐dependent luminescence properties, are reported. The materials consist of mixed‐lanthanide β‐diketonate complexes grafted into the pores of a nanosized 2,2′‐bipyridine‐5,5′‐dicarboxylate‐acid MOF. A very specific choice of lanthanides and their ratios, as well as β‐diketonate ligand, is crucial for obtaining the desired properties. The wavelength‐dependent luminescence properties of the materials are very well matched with the excitation wavelengths of a standard UV lamp, and a clearly visible change in luminescence is observed in a narrow temperature range (slightly below and above room temperature), proving them to be excellent materials for use in anti‐counterfeit technologies, which would be almost impossible to mimic. 相似文献
ABSTRACT The extraction capability of derivatives of imidazole towards Co(II) and Ni(II) has been investigated in dilute acid medium in the presence of each of chloride, perchlorate and thiocyanate ions. The influence of the stereochemistry of the substituted imidazole on the extraction behaviour has been investigated. Two-phase potentiometric titrations were employed to determine the nature of the metal species extracted into the organic phases. The electronic spectra of the extracted metal species have been employed to provide information of the nature of such species. 相似文献
ABSTRACT A number of derivatives of imidazole have been synthesized. Their extraction behaviour towards Cu2+ in dilute acid medium have been investigated in both chloride and perchlorate media. The influence of electronic and stereochemical effects are discussed. Two-phase potentiometric titrations were employed to determine the two-phase protonation constants and Cu(II) complex species present in the organic phases. The N-decylimidazole derivative has a higher extraction efficiency than the other C-ring substituted imidazoles in spite of their greater protonation constants. 相似文献
Leishmania spp. are human pathogens that utilize a novel beta-1,2-mannan as their major carbohydrate reserve material. We describe a new approach that combines traditional substrate-modification methods and "click chemistry" to assemble a library of modified substrates that were used to qualitatively define the substrate tolerance of the Leishmania beta-1,2-mannosyltransferases responsible for beta-1,2-mannan biosynthesis. The library was assembled by using the highly selective copper(I)-catalysed cycloaddition reaction of azides and alkynes to couple an assortment of azide- and alkyne-functionalized small molecules with complementary alkyne- and azide-functionalized mannose derivatives. All mannose derivatives with alpha-orientated substituents on the anomeric carbon were found to act as substrates when incubated with a Leishmania mexicana particulate fraction containing GDP-mannose. In contrast, 6-substituted mannose derivatives were not substrates. Representative products formed from the library compounds were analysed by mass spectrometry, methylation linkage analysis and beta-mannosidase digestions and showed extension with up to four beta-1,2-linked mannosyl residues. This work provides insights into the substrate specificity of this new class of glycosyltransferases that can be applied to the development of highly specific tools and inhibitors for their study. 相似文献