Enhanced sample concentration on a three-dimensional origami paper-based analytical device with non-uniform assay channel

Traditional microfluidic paper-based analytical devices (μPADs) consist of a flat straight channel printed on a paper substrate. Such devices provide a promising low-cost solution for a variety of biomedical assays. However, they have a relatively high sample consumption due to their use of external reservoirs. Moreover, in μPADs based on the ion concentration polarization (ICP) effect, controlling the cross-sectional area of the Nafion membrane relative to that of the hydrophilic channel is difficult. Accordingly, the present study utilizes an origami technique to create a μPAD with a three-dimensional (3D) structure. The μPAD features short channels and embedded reservoirs, and therefore reduces both the driving voltage requirement and the sample consumption. Moreover, the preconcentration effect is enhanced through the use of an additional hydrophilic area adjacent to the Nafion membrane. The existence of electroosmotic flow (EOF) within the proposed device is confirmed using a current-monitoring method. In addition, the occurrence of ICP is evaluated by measuring the current–voltage response of the device at external voltages ranging from 0 to 50 V. The experimental results obtained for a fluorescein sample with an initial concentration of 10^?5 M show that a 100-fold enhancement factor can be achieved given the use of a non-uniform-geometry design for the assay channel and an additional hydrophilic region with an area equal to approximately 10% of the channel cross-sectional area. Finally, a 100-fold factor can also be achieved for a fluorescein isothiocyanate sample with an initial concentration of 10^?6 M given an external driving voltage of 40 V.     

Molecular Verification of Rule-Based Systems Based on DNA Computation

Various graphic techniques have been developed to analyze structural errors in rule-based systems that utilize inference (propositional) logic rules. Four typical errors in rule-based systems are: redundancy (numerous rule sets resulting in the same conclusion); circularity (a rule leading back to itself); incompleteness (deadends or a rule set conclusion leading to unreachable goals); and inconsistency (rules conflicting with each other). This study presents a new DNA-based computing algorithm mainly based upon Adleman's DNA operations. It can be used to detect such errors. There are three phases to this molecular solution: rule-to-DNA transformation design, solution space generation, and rule verification. We first encode individual rules using relatively short DNA strands, and then generate all possible rule paths by the directed joining of such short strands to form longer strands. We then conduct the verification algorithm to detect errors. The potential of applying this proposed DNA computation algorithm to rule verification is promising given the operational time complexity of O(n*q), in which n denotes the number of fact clauses in the rule base and q is the number of rules with longest inference chain.     

Electrostatic model for an asymmetric combdrive

This paper presents an analytical solution to the electrostatic actuation of an asymmetric combdrive in out-of-plane and torsional motions. The exact solutions to force in the out-of-plane motion and the integral for torque in the torsional motion are obtained. The dependence of the peak force on the thickness of the movable fingers and the amount of overlap of the combs is given in closed form. Using our model, the shift of the natural resonant frequency due to a dc bias is analyzed. Furthermore, our solution also applies to the in-plane motion of an in-plane interdigitated combdrive. We found that an in-plane interdigitated combdrive generates a constant force within 0.1% when the minimum initial engagement length of the combs is twice the separation gap     

A Piecewise Grey System Model for Study the Effects of Anthropogenic Activities on Karst Hydrological Processes

Sustainable groundwater development must rely on a good understanding of hydrological processes, especially under effects of anthropogenic activities. This paper develops a piecewise analysis based on grey system model for study effects of anthropogenic activities on hydrological processes. The time series of precipitation and spring discharge were segmented into three time periods depending on whether variations are due to climate variation: the predevelopment stage, the transition stage, and the new equilibrium stage. Then we modeled hydrological process of the predevelopment stage and new equilibrium stage by the grey system model. By comparing the model results, we can quantify the effects of human activities on hydrological processes. We applied the model to Liulin Springs China. The results indicated that the hydraulic response time of the spring discharge to precipitation from the predevelopment stage to the new equilibrium stage, ranges from less than 1 year to up to 4 years. The results revealed that human activities slow the groundwater flow. GM (1,N) models of Liulin Springs discharge showed that the driving coefficients of precipitation to the spring discharge decreased from 0.012272 in the predevelopment stage to 0.007753 in the new equilibrium stage, which means that groundwater recharge ability has reduced about 36.82 %. Human activities in Liulin Springs have drastically changed the groundwater system. Piecewise grey system model is a robust method for hydrological process simulation.     

Penalty guided bees search for redundancy allocation problems with a mix of components in series–parallel systems

This paper uses a penalty guided strategy based on an artificial bee colony algorithm (PGBC) to solve the redundancy allocation problem (RAP) in reliability series–parallel systems. The penalty strategy was designed to eliminate the equalities in constraints and formulate new objective operators which guarantee feasibility within a reasonable execution time. The PGBC is used to deal with two kinds of RAPs with a mix of components. In the first example, the RAPs are designed to find the appropriate mix of components and redundancies within a system in order to either minimize the cost in the context of a minimum level of reliability, or maximize reliability subject to a maximum cost and weight. The second example involves RAPs of multi-state series–parallel reliability structures, wherein each subsystem can consist of a maximum of two types of redundant components. The objective is to minimize the total investment cost of system design while satisfying system reliability constraints and the consumer load demands. There are five multi-state system design problems which have been solved for illustration in this example. The experimental results show that the PGBC can significantly outperform other existing methods in the literature with less cost, higher reliability, and a significantly shorter computational time.     

Effects of the heterogeneity of game complexity and user population in learning performance of business simulation games

Previous studies on business simulation games (BSGs) have concluded that improved performance may not be the primary benefit of using BSGs, due to mixed results between student performance and perceptions. Two relevant and insightful issues attract our attention, namely, the impacts of the heterogeneous student population and the different complexity levels of BSG software. To address these issues, the present study aims to understand the relationship between student profile/characteristics and performance in the classroom with BSG-facilitated learning. An in-depth case study is conducted on a general college course designed to teach three different complexities of BSGs to students enrolled in different majors. Four student profile factors are individually tested for differences in performance scores as evaluated by the teacher. Additionally, the influences of 11 student characteristics are assessed with regard their self-reported perceived learning performances. Regression analysis and ANOVA are used to investigate the impacts of heterogeneous users and game complexity on student performance. Based on the regression analyses of the data collected from 43 respondents who participated in the general course, the study concludes that knowledge and skill may influence the heterogeneous student population; moreover, student participation and tacit learning preference improve performance, and students with an auditory learning preference or high learning motivation may not perform well in classroom BSG learning. However, the low value of adjusted R square implies that more dimensions or variables are needed to increase the explaining power of the performance scores in the regression analyses. In contrast, heterogeneous BSG software with different complexity levels present different results. The current research contributes practical and incremental knowledge on the complexity of heterogeneous BSG software on performance scores and the perceived learning performance of heterogeneous student populations. With the research limitations acknowledged, a series of suggestions for teachers pertaining to appropriate applications of BSGs in classes is offered as well as recommendations to BSG providers. Nevertheless, in-depth analyses are required, preferably with larger student population samples, to further explore the insignificant relationship between student perceptions and attitude under nonlinear extended complexity.     

Uniform parallel-machine scheduling to minimize makespan with position-based learning curves

Scheduling with learning effects has become a popular topic in the past decade; however, most of the research focuses on single-machine problems. In many situations, there are machines in parallel and the skills of workers might be different due to their individual experience. In this paper, we study a uniform parallel machine problem in which the objective is to jointly find an optimal assignment of operators to machines and an optimal schedule to minimize the makespan. Two heuristic algorithms are proposed and computational experiments are conducted to evaluate their performance.