Mesenchymal Stromal Cell Therapy in Novel Porcine Model of Diffuse Liver Damage Induced by Repeated Biliary Obstruction

In liver surgery, biliary obstruction can lead to secondary biliary cirrhosis, a life-threatening disease with liver transplantation as the only curative treatment option. Mesenchymal stromal cells (MSC) have been shown to improve liver function in both acute and chronic liver disease models. This study evaluated the effect of allogenic MSC transplantation in a large animal model of repeated biliary obstruction followed by partial hepatectomy. MSC transplantation supported the growth of regenerated liver tissue after 14 days (MSC group, n = 10: from 1087 ± 108 (0 h) to 1243 ± 92 mL (14 days); control group, n = 11: from 1080 ± 95 (0 h) to 1100 ± 105 mL (14 days), p = 0.016), with a lower volume fraction of hepatocytes in regenerated liver tissue compared to resected liver tissue (59.5 ± 10.2% vs. 70.2 ± 5.6%, p < 0.05). Volume fraction of connective tissue, blood vessels and bile vessels in regenerated liver tissue, serum levels of liver enzymes (AST, ALT, ALP and GGT) and liver metabolites (albumin, bilirubin, urea and creatinine), as well as plasma levels of IL-6, IL-8, TNF-α and TGF-β, were not affected by MSC transplantation. In our novel, large animal (pig) model of repeated biliary obstruction followed by partial hepatectomy, MSC transplantation promoted growth of liver tissue without any effect on liver function. This study underscores the importance of translating results between small and large animal models as well as the careful translation of results from animal model into human medicine.     

Effect of Erythropoietin on the Expression of Murine Transferrin Receptor 2

Erythropoietin (EPO) downregulates hepcidin expression to increase the availability of iron; the downregulation of hepcidin is mediated by erythroferrone (ERFE) secreted by erythroblasts. Erythroblasts also express transferrin receptor 2 (TFR2); however, the possible role of TFR2 in hepcidin downregulation is unclear. The purpose of the study was to correlate liver expression of hepcidin with the expression of ERFE and TFR2 in murine bone marrow and spleen at 4, 16, 24, 48, 72 and 96 h following administration of a single dose of EPO. Splenic Fam132b expression increased 4 h after EPO injection; liver hepcidin mRNA was decreased at 16 h. In the spleen, expression of TFR2 and transferrin receptor (TFR1) proteins increased by an order of magnitude at 48 and 72 h after EPO treatment. The EPO-induced increase in splenic TFR2 and TFR1 was associated with an increase in the number of Tfr2- and Tfr1-expressing erythroblasts. Plasma exosomes prepared from EPO-treated mice displayed increased amount of TFR1 protein; however, no exosomal TFR2 was detected. Overall, the results confirm the importance of ERFE in stress erythropoiesis, support the role of TFR2 in erythroid cell development, and highlight possible differences in the removal of TFR2 and TFR1 from erythroid cell membranes.     

Differential Subcellular Distribution of Cytokinins: How Does Membrane Transport Fit into the Big Picture?

Cytokinins are a class of phytohormones, signalling molecules specific to plants. They act as regulators of diverse physiological processes in complex signalling pathways. It is necessary for plants to continuously regulate cytokinin distribution among different organs, tissues, cells, and compartments. Such regulatory mechanisms include cytokinin biosynthesis, metabolic conversions and degradation, as well as cytokinin membrane transport. In our review, we aim to provide a thorough picture of the latter. We begin by summarizing cytokinin structures and physicochemical properties. Then, we revise the elementary thermodynamic and kinetic aspects of cytokinin membrane transport. Next, we review which membrane-bound carrier proteins and protein families recognize cytokinins as their substrates. Namely, we discuss the families of “equilibrative nucleoside transporters” and “purine permeases”, which translocate diverse purine-related compounds, and proteins AtPUP14, AtABCG14, AtAZG1, and AtAZG2, which are specific to cytokinins. We also address long-distance cytokinin transport. Putting all these pieces together, we finally discuss cytokinin distribution as a net result of these processes, diverse in their physicochemical nature but acting together to promote plant fitness.     

Heat Transfer in Industrial Applications—PRES 2008

This editorial provides an overview of a special issue dedicated to the 11th Conference on Process Integration, Modeling, and Optimization for Energy Saving and Pollution Reduction—PRES 2008. Nine papers have been selected and peer-reviewed covering important subjects of heat transfer engineering. They focus on recent development of various features of heat transfer equipment design and optimization. This issue of Heat Transfer Engineering is the sixth special journal issue dedicated to selected papers from PRES conferences [1 Kleme?, J. and Stehlik, P. 2002. PRES Conference—Challenges in Complex Process Heat Transfer. Heat Transfer Engineering, vol. 23: 1–2.  [Google Scholar], 2 Stehlík, P. and Kleme?, J. 2004. Selected Papers from the PRES 2002 Conference. Heat Transfer Engineering, vol. 25: 1–3.  [Google Scholar], 3 Kleme?, J. and Stehlík, P. 2005. Selected Papers from the PRES 2003 Conference. Heat Transfer Engineering, vol. 26: 1–3.  [Google Scholar], 4 Stehlík, P. and Kleme?, J. 2006. Recent Advances on Heat Transfer Equipment Design and Optimization—Selected Papers from PRES 2004 Conference. Heat Transfer Engineering, vol. 27: 1–3.  [Google Scholar], 5 Stehlík, P. and Kleme?, J. 2008. Achievements in Applied Heat Transfer—PRES 2006. Heat Transfer Engineering, vol. 29: 503–505.  [Google Scholar]].     

Development and benchmarking of a mechanical model for GFR plate-type fuel

The reference fuel design currently being considered within the Generation-IV Gas-cooled Fast Reactor (GFR) project is a ceramic plate matrix with a honeycomb inner structure containing small fuel cylinders. The fuel is mixed plutonium–uranium carbide, while the matrix material is silicon carbide. The present paper describes the mechanical part of a thermal–mechanical model being developed for studying the transient behavior of this highly heterogeneous fuel type. Benchmarking has been carried out against detailed finite-elements modeling (FEM).The resultant thermal–mechanical model can provide reliable fuel and cladding (matrix) stress/strain conditions to evaluate temperatures and neutronic feedbacks. As such, it has been integrated into PSI’s coupled code system “FAST”, which aims at the comprehensive safety analysis of advanced fast reactor systems.The detailed FEM analysis of the GFR fuel has been useful not only for benchmarking the new model, but also for obtaining an in-depth understanding of fuel stress/strain characteristics, which cannot be reproduced with simplified models. Thereby, the range of applicability of the new model has clearly been defined. In particular, the 3D FEM analysis has revealed a concentration of stresses at the pellet corners during pellet/matrix contact, which could lead to fuel element failure. This effect is found to be mitigated considerably, if the fuel pellets are shaped in a manner which enhances the contact area.     

On the Universal Computing Power of Amorphous Computing Systems

Amorphous computing differs from the classical ideas about computations almost in every aspect. The architecture of amorphous computers is random, since they consist of a plethora of identical computational units spread randomly over a given area. Within a limited radius the units can communicate wirelessly with their neighbors via a single-channel radio. We consider a model whose assumptions on the underlying computing and communication abilities are among the weakest possible: all computational units are finite state probabilistic automata working asynchronously, there is no broadcasting collision detection mechanism and no network addresses. We show that under reasonable probabilistic assumptions such amorphous computing systems can possess universal computing power with a high probability. The underlying theory makes use of properties of random graphs and that of probabilistic analysis of algorithms. To the best of our knowledge this is the first result showing the universality of such computing systems. This research was carried out within the institutional research plan AV0Z10300504 and partially supported by the GA ČR grant No. 1ET100300517 and GD201/05/H014. A preliminary, shorter version of this paper has been presented at the Third Conference on Computability in Europe, CiE 2007, Siena, Italy, June 2007 and published in the proceedings from this conference.     

The Rengen Grassland Experiment: soil contamination by trace elements after 65 years of Ca,N, P and K fertiliser application

The Rengen Grassland Experiment (RGE) was established in the Eifel Mts. (Germany) on a low productive Nardetum in 1941. Since then, the following fertiliser treatments have been applied along with a two cut system: unfertilised control, Ca, CaN, CaNP, CaNP–KCl and CaNP–K₂SO₄ with basic slag (syn. Thomas phosphate) as the only P fertiliser. The effect of long-term fertilisation on plant-available (extracted with 0.01 mol l⁻¹ CaCl₂), easily-mobilisable (extracted with 0.05 mol l⁻¹ EDTA), potentially-mobilisable (extracted with 2 mol l⁻¹ HNO₃) and total concentrations of trace elements (As, Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) in the top 0–10 and 10–20 cm of soil were investigated in 2006. According to redundancy analysis (RDA), the effect of treatment on the concentrations of risk elements was significant and explained 82.3 and 90.6% of the variability in the data in the 0–10 and 10–20 cm soil layers, respectively. Basic slag supplied the soil with considerable amounts of As, Cr, Cu, Fe, Mn and Zn. Following 65 years of fertiliser application the concentrations of risk elements in the soil profile had increased substantially, especially with basic slag. However, threshold limits for total trace element concentration in soil permitted by Czech national legislation were exceeded only in the case of As. The increase in plant-available As concentrations was most critical as it increased the potential uptake of As by plants in plots fertilised with P. Although P treatments received more than 300 g of Cr ha⁻¹ annually, no effect on plant-available Cr soil content was detected. This contrasted with the accumulation of total Cr in the 0–10 and 10–20 cm soil layers. Furthermore, plant availability of Cd, Fe, Mn and Zn was affected by soil pH and generally decreased with the application of quick lime. Plant availability of these elements was not correlated with amounts supplied by fertilisers.     

Conformation and electrical conductivity of poly(γ-methyl-L(LD)-glutamates) reticulate-doped with 1,3-dimethylimidazolium-7,7,8,8-tetracyano-p-quinodimethane complex salt

Polymeric composites were prepared by reticulate doping of poly(γ-methyl-L-glutamate) (PMLGA) and poly(γ-methyl-LD-glutamate) (PMLDGA, 1 : 1) having different conformation forms with 1,3-dimethylimidazolium-TCNQ complex salt. The heat induced α-helix to coil transition in PMLGA and α-helix to β-form and coil transition in PMLDGA were carried out in chlorobenzene and o-dichlorobenzene at temperatures between 70 and 150°C. The conductivity of polyglutamate composites thermally treated and prepared at 70°C is higher by approximately two orders of magnitude than that of thermally not treated films cast at ambient temperature. The conductivity of the most conductive reticulate-doped PMLGA film is higher by more than four orders of magnitude than that of the 1,3-dimethylimidazolium-TCNQ complex salt in pressed pellets.     

Regional differences in price levels across the European Union and their implications for its regional policy

Although regional price levels should be accounted for in any analysis of well-being in regions, the European Union fails to reflect them because they are not currently available for most EU member states. To fill this gap, we estimate regional price levels for all EU regions on the basis of available regional price level data from six EU countries. Once socio-economic indicators are recalculated using our estimates, substantially higher differences in living standards emerge. These results also have important implications for the European cohesion policy, with 9% of regions currently misclassified as a consequence of regional price levels not being used.