全文获取类型
收费全文 | 1012篇 |
免费 | 63篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 13篇 |
化学工业 | 245篇 |
金属工艺 | 8篇 |
机械仪表 | 21篇 |
建筑科学 | 27篇 |
能源动力 | 69篇 |
轻工业 | 113篇 |
水利工程 | 2篇 |
石油天然气 | 2篇 |
无线电 | 117篇 |
一般工业技术 | 170篇 |
冶金工业 | 66篇 |
原子能技术 | 11篇 |
自动化技术 | 212篇 |
出版年
2024年 | 2篇 |
2023年 | 11篇 |
2022年 | 43篇 |
2021年 | 62篇 |
2020年 | 28篇 |
2019年 | 25篇 |
2018年 | 33篇 |
2017年 | 23篇 |
2016年 | 54篇 |
2015年 | 33篇 |
2014年 | 50篇 |
2013年 | 75篇 |
2012年 | 61篇 |
2011年 | 82篇 |
2010年 | 43篇 |
2009年 | 51篇 |
2008年 | 35篇 |
2007年 | 47篇 |
2006年 | 38篇 |
2005年 | 31篇 |
2004年 | 25篇 |
2003年 | 21篇 |
2002年 | 22篇 |
2001年 | 16篇 |
2000年 | 13篇 |
1999年 | 8篇 |
1998年 | 23篇 |
1997年 | 10篇 |
1996年 | 16篇 |
1995年 | 8篇 |
1994年 | 11篇 |
1993年 | 8篇 |
1992年 | 5篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1989年 | 4篇 |
1988年 | 2篇 |
1987年 | 5篇 |
1985年 | 5篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1982年 | 4篇 |
1981年 | 2篇 |
1980年 | 4篇 |
1979年 | 3篇 |
1978年 | 5篇 |
1977年 | 2篇 |
1976年 | 4篇 |
1975年 | 2篇 |
1974年 | 4篇 |
排序方式: 共有1076条查询结果,搜索用时 359 毫秒
11.
Generalizations and unification of the celebrated Paris’ and Wöhler’s laws for fatigue crack propagation are derived by applying the recently developed quantized (or finite) fracture mechanics. In particular, three generalized Paris’, Wöhler’s or unified laws are proposed and compared, demonstrating their applicability for predicting the life time of structures containing from small (the Wöhler’s regime) to large (the Paris’ regime) propagating fatigue cracks. 相似文献
12.
The definite trend towards the use of large-diameter rebars and the introduction of high-strength steels (f
y=500 to 600 MPa) make it necessary to study the effects of longitudinal splitting on the steel-to-concrete bond. The study
of splitting effects requires firstly basic tests to be performed in order to gather experimental information on bond and
confinement stresses acting at the bar-to-concrete interface. For this purpose, three series of tests were recently carried
out at the Politecnico di Milano. The results make it possible to ascertain a few basic properties of the bond after concrete
splitting, and to formulate empirical constitutive laws regarding the stresses and the displacements (bar slip and opening
of the splitting crack). All specimens consisted of a short deformed bar embedded in a concrete block, which had a preformed
splitting crack in the plane passing through the bar axis: twelve specimens (Tests A and C) were fitted up with a round deformed
bar having crescent-shaped lugs (Db=18 mm); seven specimens (Tests B) were fitted up with a specially machined deformed bar having a rectangular cross-section
and straigth, lugs, so that concrete deterioration close to the bar could be investigated at the surface of the specimen,
by means of the moiré technique. The tests were carried out at constant slip rate, up to very large slip values (
); both the ascending and the descending branches of the stress-slip curves were measured, for four different values of the
opening of the splitting crack. The agreement among the results of the three series is generally satisfactory and often very
good: consequently, constitutive laws regarding the four main variables (crack opening and bar slip, shear and confinement
stresses) can be worked out, as will be shown in a companion paper on constitutive relationships and on concrete deterioration
at the bar-to-concrete interface. 相似文献
13.
Marta Guerini Pietro Grisoli Cristina Pane Paola Perugini 《International journal of molecular sciences》2021,22(2)
The aim of this work was the development of microstructured lipid carriers (MLC) based on chitosan (CH) and containing N-acetylcysteine (NAC), a mucolytic and antioxidant agent, to inhibit the formation of Pseudomonas aeruginosa biofilm. MLC were prepared using the high shear homogenization technique. The MLC were characterized for morphology, particle size, Z potential, encapsulation efficiency and drug release. The antioxidant properties of NAC-loaded microstructured carriers were evaluated through an in vitro spectrophotometer assay. Finally, the activity of NAC-CH-MLC on biofilm production by Pseudomonas aeruginosa was also evaluated. Results obtained from this study highlighted that the use of chitosan into the inner aqueous phase permitted to obtain microstructured particles with a narrow size range and with good encapsulation efficiency. NAC-loaded MLC showed higher antioxidant activity than the free molecule, demonstrating how encapsulation increases the antioxidant effect of the molecule. Furthermore, the reduction of biofilm growth resulted extremely high with MLC being 64.74% ± 6.2% and 83.74% ± 9.95%, respectively, at 0.5 mg/mL and 2 mg/mL. In conclusion, this work represents a favorable technological strategy against diseases in which bacterial biofilm is relevant, such as cystic fibrosis. 相似文献
14.
Davide Barbagallo Angela Caponnetto Cristina Barbagallo Rosalia Battaglia Federica Mirabella Duilia Brex Michele Stella Giuseppe Broggi Roberto Altieri Francesco Certo Rosario Caltabiano Giuseppe Maria Vincenzo Barbagallo Carmelina Daniela Anfuso Gabriella Lupo Marco Ragusa Cinzia Di Pietro Thomas Birkballe Hansen Michele Purrello 《International journal of molecular sciences》2021,22(4)
Circular RNAs (circRNAs) are a large class of RNAs with regulatory functions within cells. We recently showed that circSMARCA5 is a tumor suppressor in glioblastoma multiforme (GBM) and acts as a decoy for Serine and Arginine Rich Splicing Factor 1 (SRSF1) through six predicted binding sites (BSs). Here we characterized RNA motifs functionally involved in the interaction between circSMARCA5 and SRSF1. Three different circSMARCA5 molecules (Mut1, Mut2, Mut3), each mutated in two predicted SRSF1 BSs at once, were obtained through PCR-based replacement of wild-type (WT) BS sequences and cloned in three independent pcDNA3 vectors. Mut1 significantly decreased its capability to interact with SRSF1 as compared to WT, based on the RNA immunoprecipitation assay. In silico analysis through the “Find Individual Motif Occurrences” (FIMO) algorithm showed GAUGAA as an experimentally validated SRSF1 binding motif significantly overrepresented within both predicted SRSF1 BSs mutated in Mut1 (q-value = 0.0011). U87MG and CAS-1, transfected with Mut1, significantly increased their migration with respect to controls transfected with WT, as revealed by the cell exclusion zone assay. Immortalized human brain microvascular endothelial cells (IM-HBMEC) exposed to conditioned medium (CM) harvested from U87MG and CAS-1 transfected with Mut1 significantly sprouted more than those treated with CM harvested from U87MG and CAS-1 transfected with WT, as shown by the tube formation assay. qRT-PCR showed that the intracellular pro- to anti-angiogenic Vascular Endothelial Growth Factor A (VEGFA) mRNA isoform ratio and the amount of total VEGFA mRNA secreted in CM significantly increased in Mut1-transfected CAS-1 as compared to controls transfected with WT. Our data suggest that GAUGAA is the RNA motif responsible for the interaction between circSMARCA5 and SRSF1 as well as for the circSMARCA5-mediated control of GBM cell migration and angiogenic potential. 相似文献
15.
Margaret Ottaviano Emilio Francesco Giunta Marianna Tortora Marcello Curvietto Laura Attademo Davide Bosso Cinzia Cardalesi Mario Rosanova Pietro De Placido Erica Pietroluongo Vittorio Riccio Brigitta Mucci Sara Parola Maria Grazia Vitale Giovannella Palmieri Bruno Daniele Ester Simeone 《International journal of molecular sciences》2021,22(7)
As widely acknowledged, 40–50% of all melanoma patients harbour an activating BRAF mutation (mostly BRAF V600E). The identification of the RAS–RAF–MEK–ERK (MAP kinase) signalling pathway and its targeting has represented a valuable milestone for the advanced and, more recently, for the completely resected stage III and IV melanoma therapy management. However, despite progress in BRAF-mutant melanoma treatment, the two different approaches approved so far for metastatic disease, immunotherapy and BRAF+MEK inhibitors, allow a 5-year survival of no more than 60%, and most patients relapse during treatment due to acquired mechanisms of resistance. Deep insight into BRAF gene biology is fundamental to describe the acquired resistance mechanisms (primary and secondary) and to understand the molecular pathways that are now being investigated in preclinical and clinical studies with the aim of improving outcomes in BRAF-mutant patients. 相似文献
16.
Luisa Siculella Laura Giannotti Benedetta Di Chiara Stanca Matteo Calcagnile Alessio Rochira Eleonora Stanca Pietro Alifano Fabrizio Damiano 《International journal of molecular sciences》2021,22(8)
Reactive intermediate deaminase (Rid) proteins are enzymes conserved in all domains of life. UK114, a mammalian member of RidA subfamily, has been firstly identified as a component of liver perchloric acid-soluble proteins (L-PSP). Although still poorly defined, several functions have been attributed to the mammalian protein UK114/RIDA, including the reactive intermediate deamination activity. The expression of UK114/RIDA has been observed in some tumors, arousing interest in this protein as an evaluable tumor marker. However, other studies reported a negative correlation between UK114/RIDA expression, tumor differentiation degree and cell proliferation. This work addressed the question of UK114/RIDA expression in human non-tumor HEK293 cell lines and in some human tumor cell lines. Here we reported that human RIDA (hRIDA) was expressed in all the analyzed cell line and subjected to lysine (K-)succinylation. In HEK293, hRIDA K-succinylation was negatively correlated to the cell proliferation rate and was under the control of SIRT5. Moreover, K-succinylation clearly altered hRIDA quantification by immunoblotting, explaining, at least in part, some discrepancies about RIDA expression reported in previous studies. We found that hRIDA was able to deaminate reactive enamine-imine intermediates and that K-succinylation drastically reduced deaminase activity. As predicted by in silico analysis, the observed reduction of deaminase activity has been related to the drastic alterations of hRIDA structure inferred by K-succinylation. The role of hRIDA and the importance of its K-succinylation in cell metabolism, especially in cancer biology, have been discussed. 相似文献
17.
The report presents an innovative solar barometric distillation technology for seawater desalting (SW-SBD) which has several attractive features such as: (1) efficient vacuum solar collectors of simple construction; (2) a barometric layout for quasi-steady operation at subatmospheric pressure; (3) a demister system of simple mechanical construction; and (4) an electronic control system regulating plant productivity parameters on available solar radiation flux. The proposed seawater desalting technology consists of single-effect distillation with water vapor produced and heated at subatmospheric pressure in a solar collector loop; the basic layout, operational features, and energy efficiency are presented and analysed in detail. Subsequent reports will provide technico-economical data from prototype desalting plants in view of industrial implementation. A promising specific electric energy consumption,∼2 kWm3 offresh water produced, was found by estimating pumping power requirements of major SW-SBD plant pumps. A sizable reduction in produced water cost with respect to an existing solar seawater desalting plant in Abu Dhabi is expected on the basis of a preliminary economic evaluation for a ∼100 m3/d plant prototype. 相似文献
18.
DellaGreca M Isidori M Lavorgna M Monaco P Previtera L Zarrelli A 《Journal of chemical ecology》2004,30(4):867-879
Twenty-five 9,10-dihydrophenanthrenes, four phenanthrenes, a dihydrodibenzoxepin, and a pyrene, isolated from the wetland plant Juncus acutus, were tested to detect their effects on the green alga Selenastrum capricornutum. Nine of the compounds were isolated and identified for the first time. Most of the compounds caused inhibition of algal growth. The 9,10-dihydrophenanthrenes 1, 5, 21, and 22 were the most active. 相似文献
19.
Given a collection of parameterized multi-robot controllers associated with individual behaviors designed for particular
tasks, this paper considers the problem of how to sequence and instantiate the behaviors for the purpose of completing a
more complex, overarching mission. In addition, uncertainties about the environment or even the mission specifications
may require the robots to learn, in a cooperative manner, how best to sequence the behaviors. In this paper, we approach this
problem by using reinforcement learning to approximate the solution to the computationally intractable sequencing problem,
combined with an online gradient descent approach to selecting the individual behavior parameters, while the transitions
among behaviors are triggered automatically when the behaviors have reached a desired performance level relative to a task
performance cost. To illustrate the effectiveness of the proposed method, it is implemented on a team of differential-drive
robots for solving two different missions, namely, convoy protection and object manipulation. 相似文献
20.
Cristiano Ceglie Giuseppe Piro Domenico Striccoli Pietro Camarda 《Multimedia Tools and Applications》2016,75(8):4411-4440
The progress of three-dimensional 3D technologies, together with the wide diffusion of both Internet and broadband technologies, is paving the way to emerging live streaming services which have been conceived for delivering 3D video contents in real-time fashion to end users. Nowadays, the only available tools supporting stereoscopic 3D video services cannot be freely downloaded and require the adoption of owner stereoscopic players. Motivated by the lack of an effective solution, we developed a freeware and open source 3D live streaming framework, namely 3DStreaming. It provides stereoscopic 3D live streaming services over the Internet. In particular, it realizes a complete server implementation, offering the support for any transmission protocol and encoding scheme, as well as the full compatibility with any network architecture (i.e., LAN, MAN, Internet, and so on). At the same time, it allows users to use the preferable stereoscopic player and to render the video through any technique available for the chosen player. The overall performances of the proposed tool have been presented by testing its behavior in several network configurations (i.e., by varying network topology, coding technique, 3D representation format, and average encoding rate). All the measured metrics, which include the number of RTP segments that are transmitted and received, the frame loss ratio, and the PSNR, fully demonstrate the right behavior of the implemented tool in all the considered scenarios. We believe that, thanks to its high flexibility, this tool can be exploited by researchers working on stereoscopic-3D related issues to design, test, and evaluate novel and innovative algorithms, protocols, and network architectures. 相似文献