Modellierungs- und Simulationsverfahren für die optische Verbindungstechnik auf elektrischen Leiterplatten

Optical interconnection technology on the printed circuit board level is a key technology for future microelectronic equipment. The consideration of functional, technological, and economical requirements results in a hybrid solution, where electrical and optical interconnects are integrated into one substrate called electrical optical printed circuit board. The significant part of the entire design process for electrical optical printed circuit boards is marked by the design supporting modelling and simulation of optical interconnects. Based on an abstract model for an entire optical interconnect a simulation model for optical multimode-waveguides is presented, taking into account all significant waveguide properties. Apart from that, the modeling of active components (laser- and photo-diodes) is addressed.     

AlGaN/GaN HEMTs on SiC for high power broadband applications up to 40 GHz

An overview of properties and recent achievements for AlGaN/GaN high electron mobility transistors (HEMT) on semi-insulating SiC substrate is given towards high power and broadband applications up to a frequency of 40 GHz. Starting from epitaxial growth and process technology we present state-of-the-art power results obtained at the Fraunhofer Institute (IAF) from AlGaN/GaN HEMTs on SiC. Further, a one-stage 16 GHz MMIC power amplifier circuit with 1.6 W output power is presented. This result represents the first AlGaN/GaN MMIC on SiC fabricated in Europe.     

Elastodynamic parameters for dynamic interface fracture mechanics

Summary Dynamic extension of cracks running along curvilinear interfaces of brittle bimaterials subjected to mechanical crack surface loads and superimposed thermal strains acting along the ligament is considered. This paper especially addresses the provision and discussion of elastodynamic interface parameters in order to assess quantitatively the bimaterial fracture in view of the governing physical features: applied mechanical and thermal strain loading, existence of an interface, crack-tip velocity and curvature of the interface contour. By utilizing the linear theory of thermoelasticity and adopting Stroh's method of generalized complex potentials, from the corresponding boundary and continuity conditions vectorial Hilbert problems are derived. It is shown that the parameters of the eigenvalues and of the eigenvectors of the Hilbert problems can be interpreted as elastodynamic interface mechanics parameters reading (,v _p, _Hf , _Hf ). Generalized Dundurs parameters of dynamics (, ) and consequently an associated generalized Dundurs diagram of dynamics are proposed. While the aforementioned elastodynamic interface parameters (, ,v _p, _Hf , _Hf ) do not assume the interface to be damaged, interfaces with running interface cracks generally cause two additional interface parameters, denoted as bimaterial constants (, _Hf ), where the latter is specific to the curvature of the interface in conjunction with the velocity of the interface crack. However, the bimaterial constants (, _Hf ) can be traced back to interface parameters for an uncracked bimaterial, namely to (, _Hf ).     

Untersuchungen zur Synthese von Polyethern aus Anhydropolyolen

Studies on the Synthesis of Polyethers from Anhydropolyols . By reaction of 1,4:3,6-dianhydro-D-sorbitol with base and αω-dihaloalkanes mono and dialkyl derivatives are obtained. The use of trans-1,4-dichloro-2-butene leads to oligomers up to the heptamer which are isolated and structurally assigned. Polymerisations with super acids of substituted tetrahydrofuranes such as the 1,4-anhydro-2,3-di-O-alkyl-D-erythritols lead to functionalized poly(oxytetramethylenes). By detailed studies of reaction parameters optimized conditions are achieved, and by polymer analyses the linear structures of these polyethers are demonstrated.     

Structural and Functional Characterization of 4-Hydroxyphenylacetate 3-Hydroxylase from Escherichia coli

The hydroxylation of phenols into polyphenols, which are valuable chemicals and pharmaceutical products, is a challenging reaction. The search for green synthetic processes has led to considering microorganisms and pure hydroxylases as catalysts for phenol hydroxylation. Herein, we report the structural and functional characterization of the flavin adenine dinucleotide (FAD)-dependent 4-hydroxyphenylacetate 3-monooxygenase from Escherichia coli, named HpaB. It is shown that this enzyme enjoys a relatively broad substrate specificity, which allows the conversion of a number of non-natural phenolic compounds, such as tyrosol, hydroxymandelic acid, coumaric acid, hydroxybenzoic acid and its methyl ester, and phenol, into the corresponding catechols. The reaction can be performed by using a simple chemical assay based on formate as the electron donor and the organometallic complex [Rh(bpy)Cp*(H₂O)]²⁺ (Cp*: 1,2,3,4,5-pentamethylcyclopentadiene, bpy: 2,2′-bipyridyl) as the catalyst for FAD reduction. The availability of a crystal structure of HpaB in complex with FAD at 1.8 Å resolution opens up the possibility of the rational tuning of the substrate specificity and activity of this interesting class of phenol hydroxylases.     

Complete Photochemical Regulation of 8–17 DNAzyme Activity by Using Reversible DNA Photo-crosslinking

The regulation of DNAzyme activity is an important problem for its in vivo applications. We achieved photochemical regulation of DNAzyme activity by using reversible DNA photo-crosslinking of 3-cyanovinylcarbazole (^CNVK). The ODN containing ^CNVK photo-crosslinked to a pyrimidine base in the complementary strand after a few seconds of photoirradiation, and its photoadduct was split by photoirradiation of another wavelength. The activity of photo-crosslinked DNAzyme with ^CNVK was completely inhibited (OFF state). In contrast, after 312 nm irradiation, DNAzyme activity was recovered upon addition of a substrate strand (ON state). In addition, the photo-crosslinked DNAzyme is prone to enzymatic digestion by exonuclease. This photochemical OFF to ON switching with reversible DNA photo-crosslinking was regulated at the desired time and position; therefore, it might be possible to use it for in vivo application.     

Biosynthetic Plasticity Enables Production of Fluorinated Aurachins

Enzyme promiscuity has important implications in the field of biocatalysis. In some cases, structural analogues of simple metabolic building blocks can be processed through entire pathways to give natural product derivatives that are not readily accessible by chemical means. In this study, we explored the plasticity of the aurachin biosynthesis pathway with regard to using fluoro- and chloroanthranilic acids, which are not abundant in the bacterial producers of these quinolone antibiotics. The incorporation rates of the tested precursor molecules disclosed a regiopreference for halogen substitution as well as steric limitations of enzymatic substrate tolerance. Three previously undescribed fluorinated aurachin derivatives were produced in preparative amounts by fermentation and structurally characterized. Furthermore, their antibacterial activities were evaluated in comparison to their natural congener aurachin D.     

Rapid Discovery of Aspartyl Protease Inhibitors Using an Anchoring Approach

Pharmacophore searches that include anchors, fragments contributing above average to receptor binding, combined with one-step syntheses are a powerful approach for the fast discovery of novel bioactive molecules. Here, we are presenting a pipeline for the rapid and efficient discovery of aspartyl protease inhibitors. First, we hypothesized that hydrazine could be a multi-valent warhead to interact with the active site Asp carboxylic acids. We incorporated the hydrazine anchor in a multicomponent reaction and created a large virtual library of hydrazine derivatives synthetically accessible in one-step. Next, we performed anchor-based pharmacophore screening of the libraries and resynthesized top-ranked compounds. The inhibitory potency of the molecules was finally assessed by an enzyme activity assay and the binding mode confirmed by several soaked crystal structures supporting the validity of the hypothesis and approach. The herein reported pipeline of tools will be of general value for the rapid generation of receptor binders beyond Asp proteases.