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61.
Prostaglandin A2 (PGA2) potently inhibits cell proliferation and suppresses tumor growth in vivo, but little is known regarding the molecular mechanisms mediating these effects. Here we demonstrate that treatment of breast carcinoma MCF-7 cells with PGA2 leads to G1 arrest associated with a dramatic decrease in the levels of cyclin D1 and cyclin-dependent kinase 4 (cdk4) and accompanied by an increase in the expression of p21. We further show that these effects occur independent of cellular p53 status. The decline in cyclin D and cdk4 protein levels is correlated with loss in cdk4 kinase activity, cdk2 activity is also significantly inhibited in PGA2-treated cells, an effect closely associated with the upregulation of p21. Immunoprecipitation experiments verified that p21 was indeed complexed with cdk2 in PGA2-treated cells. Additional experiments with synchronized MCF-7 cultures stimulated with serum revealed that treatment with PGA2 prevents the progression of cells from G1 to S. Accordingly, the kinase activity associated with cdk4, cyclin E, and cdk2 immunocomplexes, which normally increases following serum addition, was unchanged in PGA2-treated cells. Furthermore, the retinoblastoma protein (Rb), a substrate of cdk4 and cdk2 whose phosphorylation is necessary for cell cycle progression, remains underphosphorylated in PGA2-treated serum-stimulated cells. These findings indicate that PGA2 exerts its growth-inhibitory effects through modulation of the expression and/or activity of several key G1 regulatory proteins. Our results highlight the chemotherapeutic potential of PGA2, particularly for suppressing growth of tumors lacking p53 function. 相似文献
62.
数字电视系统在提高图像质量方面已显示出极大的优越性。因此,世界各国电视台都已开始数字化的进程,中国也不例外。本文详细介绍了中央电视台第一个数字化演播室-800m^2演播室的视频系统,包括视频系统数字信号格式的确定,主要设备的特点以及演播室数字视频系统的主要特点等,供广大同行参考。 相似文献
63.
Q Hu M Trevisan Y Xu W Dong SA Berger SD Lyman MD Minden 《Canadian Metallurgical Quarterly》1995,95(6):2530-2538
The growth of human leukemic cells in culture and in vivo is dependent upon the presence of hematopoietic growth factors. Most populations of human leukemic acute myeloblastic leukemia (AML) cells express c-Kit on their surface and respond to Kit ligand (KL) in culture. To determine if this interaction was of potential significance in vivo we used a mouse model system. 32D cells, a murine IL-3-dependent myeloid cell line, were rendered KL responsive by transfection of the murine c-Kit. After injection of 32D or 32D-Kit cells into syngeneic hosts, animals bearing 32D-Kit cells, but not 32D cells, became moribund and were killed. These animals had circulating leukemic blast cells, infiltration of bone marrow, spleen, brain, liver, lung, and kidney. Cells recovered from some of the animals continued to be dependent upon IL-3 or KL for growth while in other cases the cells were factor independent. This model illustrates that the constitutive expression of c-Kit enhances the leukemic potential of 32D cells. The model will be useful for studying the progression of leukemia in vivo and testing whether interruption of the interaction of Kit and KL can affect the growth of leukemic cells. 相似文献
64.
The problem of joint estimation of time delay and Doppler shift is considered from the point of view of the Wigner distribution of the signal. A very efficient method of obtaining the optimum signal with minimum estimation error based on the convexity of the design region is developed. Practical applications, however, require the signal to satisfy other constraints which present complications in acquiring the optimum signal. A design approach based on the method of simulated annealing is suggested to solve for the optimum signal under constraints. The performance of the signals so obtained is evaluated and compared with that of signals obtained by synthesis 相似文献
65.
Preparation of Metal-Coated Carbon Fiber Reinforced Composites and Their Electromagnetic Properties 总被引:1,自引:0,他引:1
Medium temperature curing by infrared radiation was used to prepare electromagnetic wave absorbing multilayered plastic matrix composite materials containing metal-coated carbon fibers (MCFs). The electromagnetic properties of the materials were investigated by frequency scanning tests with microwave vector network analyzer. It is found that the electromagnetic parameters, mainly permittivity of the composites increase significantly with the addition of MCFs, which gives the composites good designability as thin, light-weight EM wave absorbers. It is also shown that MCFs can improve on the EM wave energy attenuating property of the composites by widening the work frequency band width and raising the absorptive peak 相似文献
66.
67.
On the basis of Odum's ecological economic measure of emergy as embodied solar energy, a system account of biomass resource exploitation by agriculture in China 2004 is developed in this paper, which supplements a former study on corresponding long-term historical trends during 1978 to 2000 (Chen et al., 2006. Emergy-based analysis of the Chinese agriculture. Agriculture, Ecosystems and Environment 115, 161–173). The aggregate fluxes and indicators for biomass resource exploitation in China 2004 are calculated and illustrated when compared with those for 2000 to elucidate the latest status of the Chinese agriculture as the exploitation sector for biomass resource. Data sources and algorithm are presented in detail as basic references for related analysis involving the ecological economy of biomass exploitation in agriculture. 相似文献
68.
Mode-beating spectra are investigated in long-perimeter (1.028 cm) monolithically integrated multiple-quantum-well semiconductor ring lasers. The beating line near 8 GHz is found to be split into two components. With increasing pumping current, a normal component shifts to lower frequencies, while an anomalous higher-frequency component shifts in the opposite direction. It is suggested the anomalous blue-shifting line results from power-induced perturbation of the refractive index in the vicinity of a strong mode. 相似文献
69.
In industrial systems, the formation of biofilms can cause many problems, such as an increase in the flow resistance of pipelines, energy losses in fluid transport and heat exchangers, product contamination, materials deterioration, and biocorrosion. As a result, biofilms contribute substantially to economic losses in the industry. Corrosion is particularly an issue in the petroleum industry and its implications range from down-hole completion through petroleum processing units. Much of this corrosion is attributed to microbial activities. This paper proposes a mathematical model for predicting substrate concentration for such microbial growth. Substrate concentrations in the system and near the biofilm surface are one of the parameters that has a great effect in determining the extent of the problems associated with biofilms. In this study, a convective-diffusion model under various flow conditions (stagnant, laminar, and turbulent) has been solved using the finite difference technique, employing the alternating direction implicit (ADI) method. The model assumes that a liquid containing substrate and bacteria is flowing in a pipeline with known concentrations at the inlet and then predicts the variation of the transient (as a function of time) substrate concentration along the pipeline and as a function of the pipe radius. The model is then used to predict and estimate the substrate concentration profiles on the biofilm surface under different environmental conditions. A parametric study was also conducted to study the effect of the different parameters influencing the substrate concentration profiles in the system and on the biofilm surface. 相似文献
70.