Assessment of nitrification in groundwater filters for drinking water production by qPCR and activity measurement

In groundwater treatment for drinking water production, the causes of nitrification problems and the effectiveness of process optimization in rapid sand filters are often not clear. To assess both issues, the performance of a full-scale groundwater filter with nitrification problems and another filter with complete nitrification and pretreatment by subsurface aeration was monitored over nine months. Quantitative real-time polymerase chain reaction (qPCR) targeting the amoA gene of bacteria and archaea and activity measurements of ammonia oxidation were used to regularly evaluate water and filter sand samples. Results demonstrated that subsurface aeration stimulated the growth of ammonia-oxidizing prokaryotes (AOP) in the aquifer. Cell balances, using qPCR counts of AOP for each filter, showed that the inoculated AOP numbers from the aquifer were marginal compared with AOP numbers detected in the filter. Excessive washout of AOP was not observed and did not cause the nitrification problems. Ammonia-oxidizing archaea grew in both filters, but only in low numbers compared to bacteria. The cell-specific nitrification rate in the sand and backwash water samples was high for the subsurface aerated filter, but systematically much lower for the filter with nitrification problems. From this, we conclude that incomplete nitrification was caused by nutrient limitation.     

Toxicity, accumulation and elimination studies of α-hexachlorocyclohexane (α-HCH) with freshwater organisms of different trophic levels

A short- and long-term toxicity study with α-hexachlorocyclohexane (α-HCH) was carried out with freshwater organisms of different trophic levels (algae, crustaceae and fishes). In this study mortality, immobilization, growth, reproduction, histopathologic changes and enzyme activities were chosen as criteria.Furthermore accumulation and elimination processes were investigated in separate organisms as well as the uptake of α-HCH by one organism and through a food-chain.Finally a tentative method for deriving an “ecological limit” for χ-HCH in surface water is described.     

Terrain analysis for development studies: Typical mountainous area along the Eastern Mediterranean coast-lebanon

The limited areal extent of small countries means that reliance has to be placed on mountainous terrain in developing more land needed for continuous growth. These terrains, especially along the Eastern Mediterranean, usually have rugged morphologies which makes their evaluation difficult. The Australian P.U.C.E. system for terrain assessment and classification proved to be an optimal approach for engineering-geology related studies for the above task in the Aley-Chouf area. Basic land properties having an impact on land development are singled out. They constitute mainly the geological formations, relief, drainage, land forms, soils and the vegetation cover. The land divisions used are Province, Pattern and Unit which cover ten rock formations of varying lithologies, water character and stability features. The area is divided into 7 provinces, 26 terrain patterns and 152 units. These land divisions imply finding similar engineering geological problems and applying similar solutions when planning to develop the area, or some other equivalent areas.     

Lead,cadmium, and arsenic residues in animal tissues in relation to those in their surrounding habitat

The relationships of the residues of lead, cadmium, and arsenic in animal tissues to those in surrounding vegetation and soil were determined in a natural habitat representing a wide range of such residues. The samples studied were selected from 18 different locations. Analyses were performed on a total of 113 animals, 332 plant specimens (representing 33 species), and 408 soil samples (taken at different depths). For the purpose of evaluating relationships, the samples from each location were averaged and reported as parts per million on a dry weight basis. The average lead residues from different locations ranged from 1.4–9.2 ppm in the liver, 45–195 in bones, 4–283 in vegetation, and 15–1399 in soil samples. The cadmium contents ranged from 1–27 ppm in the liver, 3–77 in bones, 0.5–5 in vegetation, and 1–10 in soil. The arsenic contents in different components of the ecosystem varied with an average of 1–9 ppm in liver, 0.6–94 in vegetation, and 7–655 in soil samples. The soil residues varied considerably according to the depth of sampling. Little variation was noted in different species of vegetation or in soil and vegetation samples according to the time and year of sampling.Statistical analyses performed on the averages indicated a significant correlation between vegetation and soil residues for all three elements, and also between animal to vegetation and animal to soil cadmium residues. Multiple linear correlations for all elements in soil, vegetation, and animal tissues were determined. The results indicated a possibility of an increase in cadmium in animal tissues depending upon the increased residues of this metal in the environment.     

The effect of temperature on the removal of non-ionic surfactants during small-scale activated-sludge sewage treatment—I. Comparison of alcohol ethoxylates with a branched-chain alkyl phenol ethoxylate

The degree of biodegradability of three non-ionic surfactants has been assessed using small-scale activated-sludge sewage treatment plants. The effect of temperature on biodegradability was studied by operation at 15°, 11° and 8°C.The two alcohol ethoxylate surfactants tested were well degraded at all three operating temperatures. The alkyl phenol ethoxylate surfactant tested was well degraded at 15°C but at lower temperatures the biodegradability was dependent on concentration. At 5 mg 1⁻¹, greater than 90 per cent removal was achieved but at 20 mg 1⁻¹ the degree of removal fluctuated between 40 and 95 per cent at 11°C and between 20 and 80 per cent at 8°C.The present results have been compared with those obtained using the same surfactants in community trials at a small sewage works employing biological filtration. Although a similar temperature effect was observed with the alkyl phenol ethoxylate, the biodegradabilities obtained in the laboratory were consistently greater than those obtained at the sewage works, perhaps because of the constant conditions of the laboratory test.     

Interstitial inorganic phosphorus concentrations in lakes Mendota and Wingra

Interstitial P levels in Lake Mendota and Lake Wingra were evaluated as a function of season and water column and sediment depth. Interstitial water was obtained by the centrifugation-filtration method. Temporal variations were observed over the entire 15 cm sediment depth interval examined in all four locations evaluated. Interstitial reactive P (IRP) levels in Lake Mendota ranged from 0.014–1.67 mg l⁻¹ at the 5–6 m water column depth and from 1.20–5.75 mg l⁻¹ at the 18–19.5 m depth. IRP levels in Lake Wingra ranged from 0.029–2.15 mg l⁻¹ at 3.5 m and from 0.191–3.96 mg l⁻¹ at 2 m. Variations in interstitial P were attributed to variations in oxidation state of Fe as influenced by oxygen transport and reduction rates.     

Structural analysis of assemblages of thin-walled members

A matrix method for the analysis of structural systems composed of thin-walled members is presented. The matrix displacement analysis includes the effects of thin-walled non-uniform torsion theory, cross-section asymmetry, eccentric restraint as well as joint types peculiar to thin-walled members. The method is used for a prediction of the elastic behaviour of a set of representative test frames. The test frames were pitched-roof portals constructed from channel sections bent about their major-axis and supported by eccentric restraints simulating purlins and girts.     

Features of disinfection kinetics of water containing <Emphasis Type="Italic">Escherichia Coli</Emphasis> in conditions of hydrodynamic cavitation

The paper has investigated the disinfecting effect of hydrodynamic cavitation on the microorganisms of E. coli in water. It has been found that disinfection kinetics depend on the intensity of the hydrodynamic cavitation field, the microbial load, and gas content. We have studied structure and morphology variation of the E. coli bacteria. It has been demonstrated that the antimicrobial effect is achieved owing to the physicochemical effects of the cavitation field, under whose influence there occurs mechanical destruction of cells and chemical disinfection by the radicals of hydroxyl and hydrogen peroxide.     

Upgrading the Activated-Sludge Process for Total Nitrogen Removal in Hong Kong

In order to protect the sensitive waters in Tolo harbour (Hong Kong), Sha Tin and Tai Po sewage-treatment works were first designed to remove 70% of the nitrogen load from the sewage. Since then, due to continuing serious eutrophication problems in the harbour, both plants have been modified to increase the removal efficiency to 90%.
The modifications were based on the Bardenpho process. However, the designers of the two plants adopted different approaches to process intensity, complexity and control of bacterial foam. At Sha Tin, the average monthly results have shown an increase of total nitrogen removal from 60–70% to about 80% since its completion. The addition of methanol was found to be ineffective on further enhancement of the denitrification rate due to difficulties in the apportioning of the second anoxic zone. The overall monthly results for Tai Po have also shown an increase in the removal rate to about 80%, even though 90% was achieved for a short period of time. The major problem encountered at the latter plant was that the process design did not provide an effective control on bacterial foaming, which had affected the smooth operation of the process.     

The Hydro-Ecology of Limestone Springs in the Wye Valley, Derbyshire

Springs are unique freshwater habitats and represent ideal sites in which to examine the relationship between environmental variables and macro-invertebrate communities. The results of three case studies infer that species assemblages are determined by the physical habitat conditions within a spring and the ecological requirements of the component species. The composition of the macro-invertebrate community changes significantly along the longitudinal gradient of a spring-brook, as predicted by ecological theory. The results of the case studies are examined in relation to the conservation and protection of these little-studied habitats, and suggestions are made as to how to achieve this in the Wye Valley.