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171.
Althaus R Torres A Peris C Beltran MC Fernandez N Molina MP 《Journal of food protection》2003,66(3):473-478
The presence of drug residues in ewe's milk samples can be determined by microbial assays. The main limitation of these tests is the large number of false-positive results associated with them. False-positive results can be explained by the interaction of certain substances naturally existing in ewe's milk with the growth of the microorganism used in the test. In this study, milk chemical composition (fat, protein, lactose, total solids), somatic cell counts (SCCs), free fatty acid concentrations, and lactoperoxidase system components were determined in order to investigate their influence on the rate of false-positive results for the BRT and Delvotest microbiological inhibitor tests. Milk samples were obtained after morning milking of Manchega ewes at 15, 30, 45, 60, 75, 90, 105, 120, and 135 days after parturition. The animals did not receive any kind of treatment or medicated feed throughout the experiment. The false-positive rates for BRT and Delvotest were 3.75 and 2.4%, respectively. When the logistic regression model was applied, the percentages of total solids for positive samples were significantly different from those for negative samples (16.90 versus 18.42% for BRT, 16.05 versus 18.45% for Delvotest), while the SCC logarithmic transformation was significantly higher for the positive samples than for the negative samples (5.38 versus 5.11 log units for BRT, 5.32 versus 5.11 log units for Delvotest). Moreover, Delvotest-positive samples exhibited thiocyanate concentrations higher than those of Delvotest-negative samples (8.18 mg/liter versus 6.85 mg/liter). Further analyses are needed to confirm the possible presence of antimicrobial residues in this particular type of milk sample. 相似文献
172.
Castillo L Martinez AI Garcerá A Elorza MV Valentín E Sentandreu R 《Yeast (Chichester, England)》2003,20(11):973-983
Identification of PIR/CIS3 gene was carried out by amino-terminal sequencing of a protein band released by beta-mercaptoethanol (beta-ME) from S. cerevisiae mnn9 cell walls. The protein was released also by digestion with beta-1,3-glucanases (laminarinase or zymolyase) or by mild alkaline solutions. Deletion of the two carboxyterminal Cys residues (Cys(214)-12aa-Cys(227)-COOH), reduced but did not eliminate incorporation of Pir4 (protein with internal repeats) by disulphide bridges. Similarly, site-directed mutation of two other cysteine amino acids (Cys(130)Ser or Cys(197)Ser) failed to block incorporation of Pir4; the second mutation produced the appearance of Kex2-unprocessed Pir4. Therefore, it seems that deletion or mutation of individual cysteine molecules does not seem enough to inhibit incorporation of Pir4 by disulphide bridges. In fks1Delta and gsc2/fks2Delta cells, defective in beta-1,3-glucan synthesis, modification of the protein pattern found in the supernatant of the growth medium, as well as the material released by beta-ME or laminarinase, was evident. However, incorporation of Pir4 by both disulphide bridges and to the beta-1,3-glucan of the cell wall continued. Deletion of the repetitive sequence (QIGDGQVQA) resulted in the secretion and incorporation by disulphide bridges of Pir4 in reduced amounts together with substantial quantities of the Kex2-unprocessed Pir4 form. Pir4 failed to be incorporated in alkali-sensitive linkages involving beta-1,3-glucan when the first repetitive sequence was deleted. Therefore, this suggests that this sequence is needed in binding Pir4 to the beta-1,3-glucan. 相似文献
173.
Asmild M Oswald N Krzywkowski KM Friis S Jacobsen RB Reuter D Taboryski R Kutchinsky J Vestergaard RK Schrøder RL Sørensen CB Bech M Korsgaard MP Willumsen NJ 《Receptors & channels》2003,9(1):49-58
Effective screening of large compound libraries in ion channel drug discovery requires the development of new electrophysiological techniques with substantially increased throughputs compared to the conventional patch clamp technique. Sophion Bioscience is aiming to meet this challenge by developing two lines of automated patch clamp products, a traditional pipette-based system called Apatchi-1, and a silicon chip-based system QPatch. The degree of automation spans from semi-automation (Apatchi-1) where a trained technician interacts with the system in a limited way, to a complete automation (QPatch 96) where the system works continuously and unattended until screening of a full compound library is completed. The performance of the systems range from medium to high throughputs. 相似文献
174.
A new analytical measurement parameter based on the effect of amphiphilic substances on the degree of binding of a surfactant to dye molecules, which induce the formation of surfactant premicellar aggregates, is presented. The theory for dye-surfactant intermolecular interactions in mixed surfactant systems, which assumes a mononuclear model for the formation of dye-induced premicellar aggregates, has been used to derive an expression that provides linear calibrations for the determination of amphiphilic compounds. The dye-surfactant interactions involved have been investigated, and the variables affecting the measurement analytical parameter have been discussed. The analytical applicability of the surfactant-dye binding degree method is demonstrated by quantifying major anionic surfactants at the nanograms-per-milliliter level and determining the total concentration of these amphiphilic substances in sewage samples (average recoveries ranged from 98 to 102%). 相似文献
175.
Rice, a cereal widely used in Venezuela for human and animal nutrition, is susceptible to aflatoxin contamination in the field and during storage. Therefore, the goal of this research was the evaluation of the efficacy and permanence of the ammoniation process through high pressure/high temperature (HP/HT) and atmospheric pressure/moderate temperature (AP/MT) conditions applied to rice samples artificially contaminated with aflatoxin B1. For this purpose a 2k design was drawn up considering the temperature, the rice moisture and the process time as variables. Under both sets of conditions, aflatoxin B1 concentration was reduced in a range of 90% to 100%. After in vitro stomach digestion simulation, toxin reversion ranged from 0% to 19%. In conclusion, the process efficacy and permanence were achieved through the use of high temperature and long process time for both sets of conditions (HP/HT and AP/MT), respectively. 相似文献
176.
Francisco J. López Fraguas Mario Rodríguez Artalejo Rafael del Vado Vírseda 《Higher-Order and Symbolic Computation》2007,20(1-2):73-122
In this paper we propose a new generic scheme CFLP풟, intended as a logical and semantic framework for lazy Constraint Functional Logic Programming over a parametrically given
constraint domain 풟. As in the case of the well known CLP풟 scheme for Constraint Logic Programming, 풟 is assumed to provide domain specific data values and constraints. CFLP풟 programs are presented as sets of constrained rewrite rules that define the behavior of possibly higher order and/or non-deterministic
lazy functions over 풟. As a main novelty w.r.t. previous related work, we present a Constraint Rewriting Logic CRWL풟 which provides a declarative semantics for CFLP풟 programs. This logic relies on a new formalization of constraint domains and program interpretations, which allows a flexible
combination of domain specific data values and user defined data constructors, as well as a functional view of constraints.
This research has been partially supported by the Spanish National Projects MELODIAS (TIC2002-01167), MERIT-FORMS (TIN2005-09207-C03-03)
and PROMESAS-CAM (S-0505/TIC/0407). 相似文献
177.
Rafael Moreno-Vozmediano Srikumar Venugopal Hussein Gibbins 《Journal of Computer and System Sciences》2007,73(8):1164-1175
The financial services industry today produces and consumes huge amounts of data and the processes involved in analysing these data have large and complex resource requirements. The need to analyse the data using such processes and get meaningful results in time, can be met only up to a certain extent by current computer systems. Most service providers attempt to increase efficiency and quality of their service offerings by stacking up more hardware and employing better algorithms for data processing. However, there is a limit to the gains achieved by using such an approach. One viable alternative would be to use emerging technologies such as the Grid. Grid computing and its application to various domains have been actively studied by many groups for more than a decade now. In this paper we explore the use of the Grid in the financial services domain; an area which we believe has not been adequately looked into. 相似文献
178.
Rafael García Victor H. López Andrew R. Kennedy Gabriel Arias 《Journal of Materials Science》2007,42(18):7794-7800
An Al-based composite reinforced with 20%SiCp was welded using the MIG welding process with direct and indirect application of the electric arc (DEA and IEA respectively).
The welds were made on 12.5 mm thick plates in three welding passes for the DEA joint whereas only one pass was required for
the IEA joint. Microstructural examination of the joints revealed DEA welds with light signs of matrix/reinforcement reaction
whilst in the IEA welds the SiC particles remained with their initial angular morphology. Mechanical failure occurred consistently
and independently of the type of joint in the weld zone and the measured strengths were 209 and 234 MPa for DEA and IEA welds
respectively. The greater strength measured for the IEA weld was due to reduced porosity and good incorporation and dispersion
of the SiC particles into the weld pool. 相似文献
179.
In the practice of project management, cost contingencies have the objective of covering probable cost increases (risks) above target estimates. Due to the variable performance nature for a wide range of activities, contingencies not only should be properly calculated but also assigned in the budget estimation process and wisely controlled during project execution. In this article, the Monte Carlo simulation approach is recommended as part of a proposed methodology for cost contingency management, which also includes a heuristics for contingency assignment (allocation) among project activities, as long as the activities have some degree of uncertainty regarding their future costs. 相似文献
180.
Large classes present many challenges in higher education. New digital technologies, such as interactive student response systems (SRSs), are promising tools for large classes. SRSs facilitate interaction between faculty members and students on an on‐going basis by allowing instructors to ask multiple‐choice, true/false and numerical questions during class and then analyse and display the student responses in real time. In general, SRSs consist of three basic components: a student input device (clicker), operating system software loaded onto the classroom computer, and an overhead projection system to display the questions asked and the distribution of student responses. SRSs have already been used in classrooms in a variety of fields; however, to our knowledge, they have not been used previously in packaging education. This article describes the introduction of an SRS in two packaging classes at the School of Packaging at Michigan State University. The SRS implementation is discussed and faculty and student comments and responses regarding the SRS are reported. The system motivated student participation and attendance for both classes: 75% of student respondents stated that the SRS motivated them to attend class; 57% of student respondents reported that the SRS enhanced their classroom experience; and 56% of the student respondents indicated that the system helped them to comprehend the material better. These numbers are supported by the vast majority of research studies, which indicate improved attendance, participation and comprehension when using SRSs for large classrooms. Copyright © 2006 John Wiley & Sons, Ltd. 相似文献