Coupling hydrothermal liquefaction and aqueous phase reforming for integrated production of biocrude and renewable H2

Lignin-rich stream from lignocellulosic ethanol production was converted into biocrude by continuous hydrothermal liquefaction (HTL) while hydrogen was produced by aqueous phase reforming (APR) of the HTL aqueous by-product. The effects of Na₂CO₃ and NaOH were investigated both in terms of processability of the feedstock as well as yield and composition of the obtained products. A maximum biocrude yield of 27 wt% was reached in the NaOH-catalyzed runs. A relevant amount of dissolved phenolics were detected in the co-produced aqueous phase (AP), and removed by liquid–liquid extraction using butyl acetate or diethyl ether, preserving the APR catalyst stability and reaching an hydrogen yield up to 146 mmol H₂ L⁻¹ AP. Preliminary mass balances integrating HTL and APR showed that the hydrogen provided by APR may account for up to 46% of the hydrogen amount theoretically required for upgrading the HTL biocrude, thus significantly improving the process performance and sustainability.     

Temperature-Dependent Re-alignment of the Short Multifunctional Peptide BP100 in Membranes Revealed by Solid-State NMR Spectroscopy and Molecular Dynamics Simulations

BP100 is a cationic undecamer peptide with antimicrobial and cell-penetrating activities. The orientation of this amphiphilic α-helix in lipid bilayers was examined under numerous conditions using solid-state ¹⁹F, ¹⁵N and ²H NMR. At high temperatures in saturated phosphatidylcholine lipids, BP100 lies flat on the membrane surface, as expected. Upon lowering the temperature towards the lipid phase transition, the helix is found to flip into an upright transmembrane orientation. In thin bilayers, this inserted state was stable at low peptide concentration, but thicker membranes required higher peptide concentrations. In the presence of lysolipids, the inserted state prevailed even at high temperature. Molecular dynamics simulations suggest that BP100 monomer insertion can be stabilized by snorkeling lysine side chains. These results demonstrate that even a very short helix like BP100 can span (and thereby penetrate through) a cellular membrane under suitable conditions.     

Activity of plant extracts for preserving functional food containing<Emphasis Type="Italic"> n</Emphasis>-3-PUFA

The ability of antioxidants obtained from natural sources to stabilise foodstuffs containing long n-3 fatty acids of marine origin has been determined. Food systems enriched in polyunsaturated fatty acids (PUFA) were: emulsified horse mackerel (Trauchurus trauchurus) muscle, fish oil-in-water emulsions (4% of n-3 PUFA) and fish oils (40% of n-3 PUFA). Rosemary leaves and extra virgin olive oil (EVO) were employed as sources of natural phenolic antioxidants. Both vegetable extracts were able to retard lipid oxidation in the different lipid systems. Rosemary extracts with a high content of carnosoic acid showed a significant synergism with fish proteins, by reinforcing their antioxidative effectiveness. Fish proteins and EVO-phenolics showed minor cooperative effects for inhibiting oxidation. The antioxidative partition into the different phases in the emulsified systems showed minor amounts of phenolics in the aqueous phases, except hydroxytyrosol and tyrosol, and a high adsorption on fish muscle.An erratum to this article can be found at     

Yeast vectors for the integration/expression of any sequence at the TYR1 locus

We have constructed new yeast vectors for targeted integration and conditional expression of any sequence at the Saccharomyces cerevisiae TYR1 locus which becomes disrupted. We show that vector integration is not neutral, causing prototrophy for tyrosine and auxotrophy for the vector's selectable marker (uracil or leucine, depending on the vector used). This feature allows a double screening of transformed yeast cells, improving the identification of colonies with the desired chromosomal structure. The GAL10 gene promoter has been added to drive conditional expression of cloned sequences. Using these vectors, chromosomal structure verification of recombinant clones is no longer necessary, since the noise of non-homologous recombination, as well as spontaneous reversion of the selected phenotype, can easily be identified. The ability of the vector to conditionally control gene expression has been confirmed using the gene for the green fluorescent protein (GFP) as a reporter.     

Overdependence on degraded gist memory in Alzheimer's disease.

Alzheimer's disease (AD) reduces associative effects on false recognition in the Deese-Roediger-McDermott task, either due to impaired memory for gist or impaired use of gist in memory decisions. Gist processes were manipulated by blocking or mixing studied words according to their associations and by varying the associative strength between studied and nonstudied words at test. Both associative blocking and associative strength had smaller effects on false recognition in AD patients than in control participants, consistent with gist memory impairments. However, unlike the case with control participants, blocking influenced true and false recognition equally in AD patients, demonstrating an overdependence on gist when making memory decisions. AD also impaired item-specific recollections, relative to control participants, as true recognition of studied words was reduced even when the two groups were equated on gist-based false recognition. We propose that the overdependence on degraded gist memory in AD is caused by even larger impairments in item-specific recollections. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Polycyclic aromatic hydrocarbon content in commercial Spanish fatty foods

The levels of polycyclic aromatic hydrocarbons (PAHs) were determined by reversed-phase high-performance liquid chromatography with fluorescence detection in different fatty foods from a Spanish market. The average concentration of the sum of total PAHs in edible vegetable oils was below 25 ng/g, whereas the sum of heavy PAHs did not surpass 5 ng/g. Olive pomace oils obtained before the summer of 2001 were an exception because they were highly contaminated. The effects of different technological processes, such as bleaching, deodorization, and hydrogenation, on PAH concentration in edible oils have been studied. The PAH profiles, as well as the influence of cooking procedures, of other fatty foods (margarine, mayonnaise, and oils from canned fishes) have been examined.     

SEL: A unified algorithm for salient entity linking

The entity linking task consists in automatically identifying and linking the entities mentioned in a text to their uniform resource identifiers in a given knowledge base. This task is very challenging due to its natural language ambiguity. However, not all the entities mentioned in the document have the same utility in understanding the topics being discussed. Thus, the related problem of identifying the most relevant entities present in the document, also known as salient entities (SE), is attracting increasing interest. In this paper, we propose salient entity linking, a novel supervised 2‐step algorithm comprehensively addressing both entity linking and saliency detection. The first step is aimed at identifying a set of candidate entities that are likely to be mentioned in the document. The second step, besides detecting linked entities, also scores them according to their saliency. Experiments conducted on 2 different data sets show that the proposed algorithm outperforms state‐of‐the‐art competitors and is able to detect SE with high accuracy. Furthermore, we used salient entity linking for extractive text summarization. We found that entity saliency can be incorporated into text summarizers to extract salient sentences from text. The resulting summarizers outperform well‐known summarization systems, proving the importance of using the SE information.     

A data fusion and spatial data analysis approach for the estimation of wheat grain nitrogen uptake from satellite data

The selection of the optimal band combination for the estimation of specific crop variables is a key aspect in order to obtain reliable estimation of in-field variability from multi- and hyperspectral remote-sensing data. The selection of the bands is strongly influenced by the phenological stage of the crop at the acquisition time. In this work, the influence of the growing stage on the combination of spectral bands related to grain nitrogen (N) uptake in wheat was evaluated using multispectral (Satellite Pour l’Observation de la Terre – SPOT) and hyperspectral (Compact High Resolution Imaging Spectrometer – CHRIS-PROBA) satellite images at different growth stages over two wheat growth seasons in central Italy. In order to identify the more appropriate covariates (spectral bands) for each phenological stage, stepwise regression with backward selection was combined with stepwise variance inflation factors (VIFs) analysis and linear mixed effect model (LMEM). The results obtained in this study suggest that the spectral region most related to N uptake varies over the growing season of the wheat crop. For SPOT data, near-infrared (NIR) region was selected at all the phenological stages in both growing seasons, except for the latest stage, with low chlorophyll content due to the onset of senescence, in which the red band was selected. At stem elongation, the shortwave infrared (SWIR) band of SPOT data was also selected. At this stage, the best N estimation accuracy was obtained using an LMEM (root mean square error, RMSE = 0.012 t ha^?1). The inclusion of a spatial component in the estimation model by means of LMEMs provided a more accurate estimation than ordinary least square (OLS) models at all growth stages. The test carried out with CHRIS-PROBA data at the fourth stage confirmed the importance of NIR and in particular of the red-edge region for N uptake prediction. A novel methodology is proposed, which involves two crucial aspects in the context of the use of remote-sensing data in precision agriculture: i) the standardization of the spatial resolution for in-field and satellite data by a geostatistical data technique (data fusion); and ii) the selection of the most appropriate spectral bands for each phenological stage, taking into account both correlation with the target variable and collinearity.     

On the Construction of Classes of Suffix Trees for Square Matrices: Algorithms and Applications

We provide a uniform framework for the study of index data structures for a two-dimensional matrixTEXT[1:n, 1:n] whose entries are drawn from an ordered alphabetΣ. An index forTEXTcan be informally seen as the two-dimensional analog of the suffix tree for a string. It allows on-line searches and statistics to be performed onTEXTby representing compactly theΘ(n³) square submatrices ofTEXTin optimalO(n²) space. We identify 4ⁿ⁻¹families of indices forTEXT, each containing ∏ⁿ_i=1 (2i−1)! isomorphic data structures. We also develop techniques leading to a single algorithm that efficiently builds any index in any family inO(n² log n) time andO(n²) space. Such an algorithm improves in various respects the algorithms for the construction of the PAT tree and the Lsuffix tree. The framework and the algorithm easily generalize tod>2 dimensions. Moreover, as part of our algorithm, we provide new algorithmic tools that yield a space-efficient implementation of the “naming scheme” of R. Karpet al.(in“Proceedings, Fourth Symposium on Theory of Computing,” pp. 125–136) for strings and matrices.     

Oxidation of 3,4-dihydroxybenzoic acid by means of hydrogen peroxide in aqueous goethite slurry

The oxidation by H2O2 of 3,4-dihydroxybenzoic acid (3,4-DHB) in aqueous/goethite slurry at varying operating conditions (catalyst load, temperature, pH, substrate and hydrogen peroxide starting concentration) is investigated. At adopted catalyst loads the observed kinetic developments are consistent with a non-radicalic oxidation mechanism involving the interaction between hydrogen peroxide and 3,4-DHB acid both adsorbed on the active sites of the catalyst. The temperature dependence of rate parameters for the proposed model is also assessed.