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111.
Gianmarco Griffini Raffaella Suriano Stefano Turri 《Polymer Engineering and Science》2012,52(12):2543-2551
The dynamic mechanical properties and electrical conductivity of a class of polyurethane fluoroelastomers filled with different carbon black types and loadings were investigated. In particular, finely structured and coarsely structured carbon blacks were considered. Dynamic mechanical analysis (DMA) on the unfilled fluoroelastomer confirmed the phase‐segregated nature of this copolymer. The dynamic mechanical behavior of elastomeric compounds reinforced with finely structured carbon black was found to be strongly influenced by the filler content above a threshold value. This behavior may be attributed to the formation of carbon black aggregates and three‐dimensional anisotropic structures at increasing filler loading. Such an effect was not observed in compounds filled with coarsely structured carbon black particles, which do not seem to form higher level structures. These observations were supported by calculations on the hydrodynamic effect of the filler on the storage modulus G′ of carbon black–loaded compounds. Electrochemical impedance spectroscopy measurements on filled fluoroelastomers revealed the presence of an electrical percolation threshold for finely structured carbon black–filled compounds that supports the hypothesis of the presence of a three‐dimensional anisotropic network forming at high filler loading. No percolation threshold was found in coarsely structured carbon black–filled compounds in accordance with DMA. These results can provide useful guidelines for the design of high‐performance carbon black–filled polyurethane fluoroelastomers. POLYM. ENG. SCI., 2012. © 2012 Society of Plastics Engineers 相似文献
112.
Vitali B Minervini G Rizzello CG Spisni E Maccaferri S Brigidi P Gobbetti M Di Cagno R 《Food microbiology》2012,31(1):116-125
This study was aimed at determining the probiotic potential of a large number of autochthonous lactic acid bacteria isolated from fruit and vegetables. Survival under simulated gastric and intestinal conditions showed that 35% of the strains, mainly belonging to the species Lactobacillus plantarum maintained high cell densities. Selected strains did not affect the immune-mediation by Caco-2 cells. All strains stimulated all 27 immune-mediators by peripheral blood mononuclear cells (PBMC). A significant (P < 0.05; P < 0.01) increase of the major part of cytokines and growth factors was found. A few chemokines were stimulated. Immune-mediators with pro-inflammatory activity (IL-17, EOTAXIN and IFNγ) were significantly (P < 0.01) stimulated by all strains, followed by IL-1b > IP-10 > IL-6 > MIP1α. Stimulation of IL-12, IL-2 and IL-7 was strain dependent. Only a few strains increased the synthesis of cytokines with anti-inflammatory activity. Six L. plantarum strains were further selected. Four were defined as the strongly adhesive strains (more than 40 bacteria adhering to one Caco-2 cell), and 2 as the adhesive strains (5–40 bacteria adhering to one Caco-2 cell). Five strains grew and acidified chemically defined medium with fructo-oligosaccharides (FOS) as the only carbon source. End-products of FOS fermentation were found. All strains inhibited enterohemorragic Escherichia coli K12 and Bacillus megaterium F6 isolated from human sources. The results of this study showed that some autochthonous lactic acid bacteria from raw fruit and vegetables have functional features to be considered as novel probiotic candidates. 相似文献
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115.
Loretta Ferrera Raffaella Barbieri Cristiana Picco Paolo Zuccolini Alessia Remigante Sara Bertelli Maria Rita Fumagalli Giovanni Zifarelli Caterina A. M. La Porta Paola Gavazzo Michael Pusch 《International journal of molecular sciences》2021,22(16)
Tumor microenvironments are often characterized by an increase in oxidative stress levels. We studied the response to oxidative stimulation in human primary (IGR39) or metastatic (IGR37) cell lines obtained from the same patient, performing patch-clamp recordings, intracellular calcium ([Ca2+]i) imaging, and RT-qPCR gene expression analysis. In IGR39 cells, chloramine-T (Chl-T) activated large K+ currents (KROS) that were partially sensitive to tetraethylammonium (TEA). A large fraction of KROS was inhibited by paxilline—a specific inhibitor of large-conductance Ca2+-activated BK channels. The TEA-insensitive component was inhibited by senicapoc—a specific inhibitor of the Ca2+-activated KCa3.1 channel. Both BK and KCa3.1 activation were mediated by an increase in [Ca2+]i induced by Chl-T. Both KROS and [Ca2+]i increase were inhibited by ACA and clotrimazole—two different inhibitors of the calcium-permeable TRPM2 channel. Surprisingly, IGR37 cells did not exhibit current increase upon the application of Chl-T. Expression analysis confirmed that the genes encoding BK, KCa3.1, and TRPM2 are much more expressed in IGR39 than in IGR37. The potassium currents and [Ca2+]i increase observed in response to the oxidizing agent strongly suggest that these three molecular entities play a major role in the progression of melanoma. Pharmacological targeting of either of these ion channels could be a new strategy to reduce the metastatic potential of melanoma cells, and could complement classical radio- or chemotherapeutic treatments. 相似文献
116.
Villa C Trucchi B Gambaro R Baldassari S 《International journal of cosmetic science》2008,30(2):139-144
Several alcohols--interesting as cosmetic fragrances whose main preparative route on an industrial scale or in the research laboratory is the reduction of the corresponding carbonyl compound--were obtained by a solvent-free methodology in a green chemistry context. The process involves the simple mixing of the carbonyl compound with sodium borohydride dispersed in wet alumina in the solid state; the conversions of the carbonyl compounds were obtained in good yields within short reaction times, without energy consumption. The following carbinols were studied: octan-3-ol, 2-cineolylols (endo-exo mixture), alpha-ionol, 4-methylbenzyl alcohol, 1-phenylethanol, trans-cinnamyl alcohol, p-anisyl alcohol, 4-phenyl-3-buten-2-ol. 相似文献
117.
Proton transverse magnetization decay curves of rice flour starch–water samples were measured and analysed for the presence of four components in the relaxation curve. T2 values were interpreted on the basis of the diffusive and chemical exchange model that provided evidence for extra granular bulk water and three more water populations whose relaxation rate is governed by diffusive and chemical exchange with starch components. The analysis of relaxation data provided information on dynamics of water molecules as well as on the size and dispersion of diffusive domains. Furthermore, by measuring solid to liquid ratio, transverse and longitudinal relaxation curves of starch–water mixtures at increasing temperatures – from 20 to 77 °C – swelling and gelatinisation processes were monitored. 相似文献
118.
Laura Micheli Emma Mitidieri Carlotta Turnaturi Domenico Vanacore Clara Ciampi Elena Lucarini Giuseppe Cirino Carla Ghelardini Raffaella Sorrentino Lorenzo Di Cesare Mannelli Roberta dEmmanuele di Villa Bianca 《International journal of molecular sciences》2022,23(11)
Sarcopenia is a gradual and generalized skeletal muscle (SKM) syndrome, characterized by the impairment of muscle components and functionality. Hydrogen sulfide (H2S), endogenously formed within the body from the activity of cystathionine-γ-lyase (CSE), cystathionine- β-synthase (CBS), and mercaptopyruvate sulfurtransferase, is involved in SKM function. Here, in an in vitro model of sarcopenia based on damage induced by dexamethasone (DEX, 1 μM, 48 h treatment) in C2C12-derived myotubes, we investigated the protective potential of exogenous and endogenous sources of H2S, i.e., glucoraphanin (30 μM), L-cysteine (150 μM), and 3-mercaptopyruvate (150 μM). DEX impaired the H2S signalling in terms of a reduction in CBS and CSE expression and H2S biosynthesis. Glucoraphanin and 3-mercaptopyruvate but not L-cysteine prevented the apoptotic process induced by DEX. In parallel, the H2S-releasing molecules reduced the oxidative unbalance evoked by DEX, reducing catalase activity, O2− levels, and protein carbonylation. Glucoraphanin, 3-mercaptopyruvate, and L-cysteine avoided the changes in myotubes morphology and morphometrics after DEX treatment. In conclusion, in an in vitro model of sarcopenia, an impairment in CBS/CSE/H2S signalling occurs, whereas glucoraphanin, a natural H2S-releasing molecule, appears more effective for preventing the SKM damage. Therefore, glucoraphanin supplementation could be an innovative therapeutic approach in the management of sarcopenia. 相似文献
119.
Use of sourdough fermented wheat germ for enhancing the nutritional, texture and sensory characteristics of the white bread 总被引:3,自引:1,他引:3
Carlo Giuseppe Rizzello Luana Nionelli Rossana Coda Raffaella Di Cagno Marco Gobbetti 《European Food Research and Technology》2010,230(4):645-654
Lactobacillus plantarum LB1 and Lactobacillus rossiae LB5, isolated from wheat germ and selected based on the kinetics of acidification, were used as starters for the manufacture
of sourdough fermented wheat germ. A bread containing sourdough fermented wheat germ as an ingredient (SFWGB) was compared
to breads made with (raw wheat germ bread, RWGB) or without (wheat flour bread, WFB) raw wheat germ. The higher concentration
of free amino acids mainly differentiated SFWGB from WFB and RWGB. The in vitro protein digestibility of WFB was the highest,
even if sourdough fermentation of wheat germ attenuated the difference. Phytase and antioxidant activities of SFWGB were highest.
The specific volume and cell-total areas were also the highest for SFWGB. As determined by texture profile analysis, the values
of hardness, resilience and fracturability of breads containing wheat germ were lower than those found in WFB. The crust lightness
showed a decrease from WFB to SFWGB. As determined by sensory analysis, SFWGB had mainly acid taste and flavour and resulted
more salty. Sourdough fermented wheat germ is an ingredient able to enhance nutritional, texture and sensory properties of
bread. 相似文献
120.
Clara Bernardelli Silvia Ancona Melania Lazzari Antonella Lettieri Piera Selvaggio Valentina Massa Cristina Gervasini Fabiano Di Marco Raffaella Chiaramonte Elena Lesma 《International journal of molecular sciences》2022,23(13)
Senescence is a stress-response process characterized by the irreversible inhibition of cell proliferation, associated to the acquisition of a senescence-associated secretory phenotype (SASP), that may drive pathological conditions. Lymphangioleiomyomatosis (LAM) is a rare disease in which LAM cells, featuring the hyperactivation of the mammalian Target of Rapamycin Complex 1 (mTORC1) for the absence of tuberin expression, cause the disruption of the lung parenchyma. Considering that LAM cells secrete SASP factors and that mTOR is also a driver of senescence, we deepened the contribution of senescence in LAM cell phenotype. We firstly demonstrated that human primary tuberin-deficient LAM cells (LAM/TSC cells) have senescent features depending on mTOR hyperactivation, since their high positivity to SA-β galactosidase and to phospho-histone H2A.X are reduced by inducing tuberin expression and by inhibiting mTOR with rapamycin. Then, we demonstrated the capability of LAM/TSC cells to induce senescence. Indeed, primary lung fibroblasts (PLFs) grown in LAM/TSC conditioned medium increased the positivity to SA-β galactosidase and to phospho-histone H2A.X, as well as p21WAF1/CIP1 expression, and enhanced the mRNA expression and the secretion of the SASP component IL-8. Taken together, these data make senescence a novel field of study to understand LAM development and progression. 相似文献