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51.
Tamara Schad Natalie Preisig Wiebke Drenckhan Cosima Stubenrauch 《Journal of surfactants and detergents》2022,25(3):377-385
Liquid foams of intermediate stability have been shown to be very efficient in the cleaning of sensitive surfaces because of the synergy between imbibition and foam decay. While we quantified these mechanisms for contaminations with liquid oils in our previous work, we show here their extension to oils containing soot particles in an effort to simulate increasingly realistic contaminations. Using foams with a wide range of liquid fractions and with different stabilities, we show that the main cleaning mechanisms remain very similar, with the oil entraining the soot particles. However, we find much less efficient soot removal when the liquid channels of the foams are small enough to hinder efficient transport of the soot particles. 相似文献
52.
Petra Fallier-Becker Maike Nieser Ulrike Wenzel Rainer Ritz Susan Noell 《International journal of molecular sciences》2016,17(8)
The astrocytic endfoot membranes of the healthy blood-brain barrier—contacting the capillary—are covered with a large number of the water channel aquaporin 4 (AQP4). They form orthogonal arrays of particles (OAPs), which consist of AQP4 isoform M1 and M23. Under pathologic conditions, AQP4 is distributed over the whole cell and no or only small OAPs are found. From cell culture experiments, it is known that cells transfected only with AQP4-M1 do not form OAPs or only small ones. We hypothesized that in astrocytomas the situation may be comparable to the in vitro experiments expecting an upregulation of AQP4-M1. Quantitative Real-time PCR (qRT-PCR) of different graded astrocytomas revealed an upregulation of both isoforms AQP4 M1 and M23 in all astrocytomas investigated. In freeze fracture replicas of low-grade malignancy astrocytomas, more OAPs than in high-grade malignancy astrocytomas were found. In vitro, cultured glioma cells did not express AQP4, whereas healthy astrocytes revealed a slight upregulation of both isoforms and only a few OAPs in freeze fracture analysis. Taken together, we found a correlation between the decrease of OAPs and increasing grade of malignancy of astrocytomas but this was not consistent with an upregulation of AQP4-M1 in relation to AQP4 M23. 相似文献
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54.
Ahmed El‐Hadi Rainer Schnabel Ekkehard Straube Gert Müller M. Riemschneider 《大分子材料与工程》2002,287(5):363-372
Poly(3‐hydroxybutyrate) (PHB) is sensitive to high processing temperatures. This leads to a decrease in molar mass as well as a lower melt viscosity. The crystallization temperature shifts to lower values, and crystallization kinetics is slow. A mixture was developed in order to improve the manufacturing properties and the final product. The blends exhibit a slight reduction in molar mass because they have a lower melting point than pure PHB, and can be extruded at their melt temperature of 170 to 180°C. Then they immediately crystallize at 125 to 100°C. Differential scanning calorimetry (DSC) shows the effect of holding time in the melt on crystallization behavior. It has been shown that the crystallization time has to be longer in the case of PHB and shorter for the blends. Thermal degradation of PHB and its blends has been investigated using thermogravimetry analysis (TG). Derivative thermogravimetry coupled with TG (TG/DTG) curves show three decomposition stages for blends at 290, 340 and 445°C, respectively. Acetic acid, water, carbon dioxide and methane are produced by degradation at a higher temperature. 相似文献
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56.
Schmidt Uli; Rudolph Rainer; Bohm Gerald 《Protein engineering, design & selection : PEDS》2001,14(10):769-774
The development of novel delivery systems for therapeutic substancesincludes targeting of the carriers to a specific site or tissuewithin the body of the recipient. This can be accomplished byappropriate receptor-binding domains and requires linking ofthese domains to the carrier. We have used recombinantly expressedpolyomavirus-like particles as a model system and inserted thesequence of a WW domain into different surface loops of theviral capsid protein VP1. In one variant, the WW domain maintainedits highly selective binding properties of proline-rich ligandsand showed an increased affinity but also an accelerated association/dissociationequilibrium compared to the isolated WW domain, thus allowinga short-term coupling of external ligands onto the surface ofthe virus-like particles. 相似文献
57.
Richter Susanne A.; Stubenrauch Kay; Lilie Hauke; Rudolph Rainer 《Protein engineering, design & selection : PEDS》2001,14(10):775-783
The de novo design of a molecular adapter for directed associationand covalent linkage of two polypeptides is presented. Usingpeptides containing charged amino acid residues and an additionalcysteine residue (AlaCysLys8 and AlaCysGlu8) we demonstratethat the electrostatic interaction promotes the associationof two synthetic peptides and, subsequently, disulfide bondformation. The reaction depends on both the redox potentialand on the ionic strength of the buffer. Varying the redox potential,the interaction of the peptides was quantified by a G0' of 6.6± 0.2 kcal/mol. Heterodimerization of the peptides ishighly specific, a competition of association by other cysteinecontaining compounds could not be observed. Two proteins comprisingcysteine-containing polyionic fusion peptides, a modified Fabfragment and an -glucosidase fusion, could be specifically conjugatedby directed association and subsequent disulfide bond formation.Both proteins retain their functional characteristics withinthe bifunctional conjugate: enzymatic activity of the glucosidaseand antigen-binding capacity of the Fab fragment are equivalentto the non-conjugated components. 相似文献
58.
Hisashi Kaga Ellen M. Heian Zuhair A. Munir Clemens Schmalzried Rainer Telle 《Journal of the American Ceramic Society》2001,84(12):2764-2770
The synthesis of solid solutions of (Ti,W,Cr)B2 from elemental reactants using the field-activated, pressure-assisted synthesis method and employing the SPS apparatus was investigated. The nature of the products depended on temperature; they were nearly pure solid solutions at 1900°C with minor amounts of β-WB. The product density and microhardness depended on the temperature of synthesis for the same value of applied pressure (64 MPa). Samples with the highest density (94%) corresponded to a hardness of 22.7 GPa. When annealed at 1500°C, the solid solutions decomposed, precipitating a (W,Ti,Cr)B2 phase in a spinodal form. In addition, β-WB precipitates in the form of thin (0.4–5.3 nm) layers were observed. They existed in a 60°/120° orientation to the (Ti,W,Cr)B2 matrix, in agreement with previous observations. Highly faceted, small (nanosized) pores associated with the β-WB precipitates were also observed. 相似文献
59.
Rainer Dahlhaus 《时间序列分析杂志》2012,33(1):13-31
In this article, we consider frequency and phase estimation in a noisy oscillation with potentially non‐constant phase increments resulting from an underlying non‐constant frequency. A maximum periodogram method on segments is used to estimate the time‐varying frequency and a subsequent least squares approach to estimate the phase. A key problem addressed in this article is the question how to set up a meaningful concept of asymptotic statistics for this model. This problem is solved by a special infill asymptotics concept. We use this concept to prove consistency and asymptotic normality of the estimates. Furthermore, the phase estimate is compared to the Hilbert transform in a simulation. 相似文献
60.