Polymers used to influence cell fate in 3D geometry: New trends

The extracellular matrix (ECM) is a hydrogel-like structure comprised of several different biopolymers, encompassing a wide range of biological, chemical, and mechanical properties. The composition, organization, and assembly of the ECM play a critical role in cell function. Cellular behavior is guided by interactions that occur between cells and their local microenvironment, and this interrelationship plays a significant role in determining physiological functions. Bioengineering approaches have been developed to mimic native tissue microenvironments by fabricating novel bioactive hydrogel scaffolds. This review explores material designs and fabrication approaches that are guiding the design of hydrogels as tissue engineered scaffolds. As the fundamental biology of the cellular microenvironment is often the inspiration for material design, the review focuses on modifications to control bioactive cues such as adhesion molecules and growth factors, and summarizes the current applications of biomimetic scaffolds that have been used in vitro as well as in vivo.     

Construction of an expression system of insect lysozyme lacking thermal stability: the effect of selection of signal sequence on level of expression in the Pichia pastoris expression system

Expression systems of human and silkworm lysozymes were constructedusing the methylotrophic yeast Pichia pastoris as a host. Theleader sequence and its prepro peptide of     

Application of magnetic pulse welding technique for flexible printed circuit boards (FPCB) lap joints

The magnetic pulse welding (MPW) is a high speed solid state welding process which has been used successfully to provide metallurgical and electrical bonds between flexible printed circuit board (FPCB) with using: (1) welding with aluminum driver sheet and (2) without driver sheet. The optimum bank energy for reliable bonding was about 1 kJ with 160–180 kA maximum current. The maximum tensile shearing for most welded samples was nearly same as tensile shearing strength of copper layer of FPCB sheet.     

Detection of Verotoxigenic Escherichia coli O157 and O26 in food by plating methods and LAMP method: a collaborative study

In order to establish a rapid and sensitive method for the detection of Verotoxigenic Escherichia coli O157 and O26, a collaborative study was conducted focusing on a comparison of the efficiency of loop-mediated amplification (LAMP) assay targeting the Verocytotoxin (also called Shiga toxin) gene, utilizing a direct plating method and a plating method with immunomagnetic separation (IMS-plating method) using various agar media. In combination with enrichment with the modified EC supplemented with novobiocin, E. coli O157 was detected in most samples of ground beef and alfalfa sprouts by LAMP assay, the direct plating method and the IMS-plating method. E. coli O26 was detected in approximately 100% of the food samples by LAMP assay. However, the IMS-plating and direct plating methods recovered 80 and 50% in ground beef samples, respectively. As a result, it was demonstrated the LAMP assay is superior to the IMS-plating method. Based on these results, it appears LAMP assay is effective as a screening assay to detect E. coli O157 and O26 from positive samples.     

Single-molecular AFM probing of specific DNA sequencing using RecA-promoted homologous pairing and strand exchange

The specific sequence in a linearlized double-stranded DNA target has been identified at a single-molecular level by atomic force microscopy (AFM). This was accomplished using RecA-coated, single-stranded DNA probes which were paired with a specific complementary DNA sequence in a linear double-stranded DNA target by strand-exchange reaction at a homologous sequence site with target DNA. The sites of interaction between the nucleoprotein filaments and the double-stranded DNA targets were directly visualized by AFM in solution containing 4 mM magnesium acetate. Measurements of the position of RecA-coated probes paired to individual target DNA showed that DNA probes specifically paired at their corresponding homologous target sequences. Strand exchange promoted by RecA and the visualization by AFM provided a rapid and efficient way to identify homologous sequence on a single-molecule target DNA.     

Extracellular matrix survival signals transduced by focal adhesion kinase suppress p53-mediated apoptosis

In many malignant cells, both the anchorage requirement for survival and the function of the p53 tumor suppressor gene are subverted. These effects are consistent with the hypothesis that survival signals from extracellular matrix (ECM) suppress a p53-regulated cell death pathway. We report that survival signals from fibronectin are transduced by the focal adhesion kinase (FAK). If FAK or the correct ECM is absent, cells enter apoptosis through a p53-dependent pathway activated by protein kinase C lambda/iota and cytosolic phospholipase A2. This pathway is suppressible by dominant-negative p53 and Bcl2 but not CrmA. Upon inactivation of p53, cells survive even if they lack matrix signals or FAK. This is the first report that p53 monitors survival signals from ECM/FAK in anchorage- dependent cells.     

Expectancy theory prediction of the goal theory postulate, "The harder the goals, the higher the performance."

Goal theory postulates that harder goals lead to higher performance than do easier goals. The present study tested the prediction, based on expectancy valence theory, that this would be true only if the payoff for succeeding at the harder goal is sufficiently greater than the alternatives to compensate for its greater difficulty. 63 undergraduates were each given an easy and a hard task/goal, requiring the comparison of paired sets of 3-digit numbers. Expectancy theory measures for the 2 goals were obtained from Ss. Performance was higher for the hard goal than for the easy goal, supporting the goal theory postulate. Force was also higher for the hard goal than for the easy goal. In addition, force change across the 2 goals was associated with performance change, supporting the conclusion that expectancy valence theory can predict the goal theory postulate. The valence of goal attainment was higher for the hard goal than for the easy goal. Valence change across the 2 goals was associated with performance change to a greater degree than was expectancy change, suggesting that the attained performance difference can be attributed to the valence difference. (10 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Investigation of the voltage life of mica-alumina composite insulation at high temperatures

Experiments and investigations were carried out on the voltage life of a bar-coil model with a nickel-plated copper conductor covered with mica-alumina composite insulation at a temperature range of 550–850 °C. Diffusion of copper into the insulation layer was observed on the specimens aged for a long period. Insulation with mica paper gave faster diffusion speed and shorter voltage life than insulation with mica flake. The diffusion speed became higher with an increase in temperature. The ac current gradually increased with aging time and increased rapidly just before breakdown. There was the relationship I = aEⁿ (a, n: constants) between ac current I and applied stress E, and n was approximately equal to 1 in the region where thermal unbalance did not occur. Arrhenius's law held in the relationship between ac current and aging time. Therefore, the breakdown mechanism might be that the effective insulation thickness decreased due to copper diffusion into the insulation layer and the ac current increased gradually until thermal breakdown in the last stage. If copper diffuses into the insulation layer, even with no voltage application, the aging time required to decrease the breakdown voltage to a certain level (the voltage life) obeys Arrhenius's law. If the voltage life is dominated by diffusion into the insulation layer, the activation energy for voltage life in the aging test becomes twice that for diffusion, both with and without voltage application. © 1999 Scripta Technica, Electr Eng Jpn, 129(4): 24–31, 1999     

A technique of waveform judgment for harmonic analysis on distribution lines

Most general‐purpose analyzers for harmonic analysis on distribution lines are designed to sample the data of one or two power supply periods. In other words, these analyzers process waveforms and display the results based on the assumption that the analyzed waveforms contain no noninteger components, such as subharmonics and interharmonics. When waveforms containing noninteger components are analyzed with these general‐purpose analyzers, the results often indicate components that are not actually present. This paper proposes a technique of waveform judgment and explains its method of application. The technique allows noninteger components to be recognized in analyzed waveforms from the properties of Lissajous figures, and clarifies the types of components and the time windows necessary for appropriate analysis. © 2008 Wiley Periodicals, Inc. Electr Eng Jpn, 166(2): 32– 38, 2009; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20536