首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   288篇
  免费   8篇
电工技术   2篇
化学工业   70篇
机械仪表   3篇
建筑科学   10篇
能源动力   7篇
轻工业   53篇
水利工程   1篇
无线电   10篇
一般工业技术   38篇
冶金工业   62篇
原子能技术   9篇
自动化技术   31篇
  2023年   1篇
  2022年   6篇
  2021年   5篇
  2020年   1篇
  2019年   4篇
  2018年   4篇
  2017年   6篇
  2016年   7篇
  2015年   2篇
  2014年   4篇
  2013年   20篇
  2012年   11篇
  2011年   19篇
  2010年   11篇
  2009年   8篇
  2008年   12篇
  2007年   14篇
  2006年   10篇
  2005年   10篇
  2004年   10篇
  2003年   15篇
  2002年   9篇
  2001年   9篇
  2000年   6篇
  1999年   5篇
  1998年   20篇
  1997年   15篇
  1996年   11篇
  1995年   4篇
  1994年   6篇
  1993年   6篇
  1992年   1篇
  1991年   6篇
  1990年   2篇
  1989年   2篇
  1988年   1篇
  1987年   1篇
  1986年   1篇
  1985年   2篇
  1984年   1篇
  1982年   1篇
  1981年   1篇
  1980年   2篇
  1977年   3篇
  1968年   1篇
排序方式: 共有296条查询结果,搜索用时 15 毫秒
291.
The binding property of bovine IgG2 to staphylococcal Protein A was investigated by the methods of gel filtration chromatography and affinity chromatography. High performance gel filtration chromatography was carried out using TSK gel G3000SW and G2000SW columns, and immobilized Protein A column was used for affinity chromatography. Although bovine IgG2 did not form any precipitin lines with Protein A by double diffusion method on agar gel, IgG2 could bind to immobilized Protein A column. Moreover, by gel filtration chromatography, peaks of the complex between bovine IgG2 and Protein A were observed in addition to the IgG2 monomer peak. Thus, it is concluded that bovine IgG2 interacts with staphylococcal Protein A and forms "soluble complexes". Carbethoxylated IgG2 lost its affinity to Protein A indicating that histidyl residues in IgG2 is essential for the binding to Protein A.  相似文献   
292.
Migration from can-coatings into retorted canned food simulants (canned oil and water, 121 degrees C, 30 min) was investigated through HPLC with a fluorescence detector and evaporative light scattering detector, and by measurements of residue on evaporation and consumption of potassium permanganate. HPLC analysis revealed that migration into the canned oil was hundreds of times more than that into n-heptane (25 degrees C, 60 min, the official test conditions according to the Japanese Food Sanitation Law), whereas it was similar to the migration into isooctane-butyl acetate mixtures (60 degrees C, 60 min), and that migration into the canned water was several times more than that into water (95 degrees C, 30 min, the official test conditions). Residue on evaporation for the n-heptane extract was several-fold lower than 30 ppm (the official limit), whereas that for the isooctane-butyl acetate mixtures exceeded 30 ppm. Consumption of potassium permanganate for the canned water was 30 times higher than that for the water extract (95 degrees C, 30 min). The official test conditions for can-coatings, in particular the use of n-heptane as an oil simulant, were suggested to lead to substantial underestimation of migration into canned food.  相似文献   
293.
Anomalous heat effect by interaction of hydrogen isotope gas and metal nanocomposites supported by zirconia or by silica has been examined. Observed absorption and heat evolution at RT were not too large to be explained by some chemical processes. At elevated temperatures of 200–300 °C, most samples with binary metal nanocomposites produced excess power of 3–24 W lasting for up to several weeks. The excess power was observed not only in the D-Pd·Ni system but also in the HPd·Ni system and HCu·Ni system, while single-element nanoparticle samples produced no excess power. The Pd/Ni ratio is one of the keys to increase the excess power. The maximum phase-averaged excess heat energy exceeded 270 keV/D, and the integrated excess heat energy reached 100 MJ/mol-M or 90 MJ/mol-H. It is impossible to attribute the excess heat energy to any chemical reaction; it is possibly due to radiation-free nuclear process.  相似文献   
294.
The model-driven architecture focuses on the evolution and integration of applications across heterogeneous platforms by means of generating implementations from platform-independent models. Most of the existing realizations of this idea are limited to static models. We propose a model-driven approach to the development of reactive information systems, like dynamic Web pages or Web services, modeling their typical request-query-update-response pattern by means of graph transformation rules. Rather than generating executable code from these models we focus on the verification of the consistency between different sub-models and an implementation that may have been produced manually. The main technical tool for achieving this goal is a mapping of graph transformation rules to contracts expressed in the Java Modeling Language.  相似文献   
295.
Pleural mesothelial cells (PMCs) play a central role in the progression of pleural fibrosis. As pleural injury progresses to fibrosis, PMCs transition to mesenchymal myofibroblast via mesothelial mesenchymal transition (MesoMT), and produce extracellular matrix (ECM) proteins including collagen and fibronectin (FN1). FN1 plays an important role in ECM maturation and facilitates ECM-myofibroblast interaction, thus facilitating fibrosis. However, the mechanism of FN1 secretion is poorly understood. We report here that myosin 5b (Myo5b) plays a critical role in the transportation and secretion of FN1 from human pleural mesothelial cells (HPMCs). TGF-β significantly increased the expression and secretion of FN1 from HPMCs and facilitates the close association of Myo5B with FN1 and Rab11b. Moreover, Myo5b directly binds to GTP bound Rab11b (Rab11b-GTP) but not GDP bound Rab11b. Myo5b or Rab11b knockdown via siRNA significantly attenuated the secretion of FN1 without changing FN1 expression. TGF-β also induced Rab11b-GTP formation, and Rab11b-GTP but not Rab11b-GDP significantly activated the actin-activated ATPase activity of Myo5B. Live cell imaging revealed that Myo5b- and FN1-containing vesicles continuously moved together in a single direction. These results support that Myo5b and Rab11b play an important role in FN1 transportation and secretion from HPMCs, and consequently may contribute to the development of pleural fibrosis.  相似文献   
296.
The complex [Ir(cod)Cl]2/DPPF or rac‐BINAP is an efficient catalyst for the [2+2+2] cycloaddition of α,ω‐diynes with cyanamides. A wide range of cyanamides derived from secondary amines are good coupling partners for α,ω‐diynes. The reaction of unsymmetrical α,ω‐diynes possessing two different internal alkyne moieties with cyanamides is regioselective. A competitive experiment showed that cyanamide is more reactive than nitrile. This higher reactivity of cyanamide than nitrile was analyzed based on density functional theory (DFT) calculations at the B3LYP level.

  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号