Antifeedant and toxic effects of sesquiterpenes fromSenecio palmensis to colorado potato beetle

A bioassay-guided fractionation of the aerial parts ofSenecio palmensis resulted in the isolation of two sesquiterpenes, 2,10-bisaboladien-1-one and 11-acetoxy-5-angeloyloxy-silphinen-3-one. The bisabolene and the silphinene represented 0.012% and 0.024% of the plant dry weight, respectively. Both compounds showed antifeedant activity againstLeptinotarsa decemlineata larvae and adults in short-term choice and no-choice bioassays. Both compounds were also tested against different species of phytopathogenic fungi. The beetles were more sensitive to these compounds in choice than in no-choice assays, with a gradient of increasing sensitivity from second instars to adults. Bisabolene was 45 times less active as an antifeedant than juglone, which was tested as a positive control. The silphinen was more active than the bisabolene, with a range of activity similar to juglone. Furthermore, exposure of fourth instars to these compounds over a 24-hr period resulted in reduced feeding and growth rates. To distinguish between antifeedant and toxic effects, growth efficiencies were calculated as the slope of the regression of relative growth rate on relative consumption rate. The comparison of these results with those of antifeedant simulation and contact toxicity bioassays indicates that feeding inhibition is the primary mode of action of the bisabolene, while the silphinene shows both antifeedant and toxic effects.     

Estimating energy intake of urban women in Colombia: comparison of diet records and recalls

A total of 611 Schistosoma mansoni infected primary school children from three schools in north-east Ethiopia were treated with praziquantel at 40 mg/kg body weight in a single dose. Pre-treatment, 40.4% had no presenting symptoms and 30-40% had nausea, abdominal cramps and/or bloody-mucoid diarrhoea. None of the pre-treatment symptoms was related to nutritional status, intensity of S. mansoni egg excretion, or to the presence of other concomitant intestinal parasitic infections. During the first 4-6 h post-treatment observation period, 90 (14.7%) children self-presented with severe gastro-intestinal symptoms. Children who self-presented with severe symptoms had a higher mean age and mean S. mansoni egg excretion compared with children who did not self-present. The following day a total of 529 (86.6%) children, including all who self-presented during the first 4-6 h post-treatment, reported for clinical check-up and were subjected to a structured questionnaire interview on symptoms they had experienced over the time lapse following treatment. Among these, 91.5% reported one or more treatment related symptoms which were at times severe. Abdominal cramps (86.9%), diarrhoea with blood and/or mucus (49.5%), dizziness (31.2%) and vomiting (24.9%) were the most common treatment related symptoms. Skin rash with oedema were observed in four cases. Among treatment related symptoms, the combination of abdominal cramps with vomiting, bloody diarrhoea, vomiting alone and general weakness were significantly higher among the malnourished. A proportion of these symptoms increased with increasing categories of S. mansoni egg excretion before and after adjusting for nutritional status and concurrent intestinal parasitic infections. Overall, the cure rate of praziquantel, among 541 children who had stool examination 5 weeks after treatment was 83.2% and this rate decreased with increasing pre-treatment egg counts. In conclusion, most of the treatment related symptoms were mild. However, some of the objective symptoms were at times severe and may reduce drug compliance in primary health care based population chemotherapy.     

The importance of orientation in proton transport of a polymer film based on an oriented self-organized columnar liquid-crystalline polyether

We prepared membranes based on a liquid-crystalline side-chain polyether obtained by chemical modification of commercial poly(epichlorohydrin) (PECH) with dendrons. This polymer exhibited a columnar structure, which could form an ion channel in the inner part. The columns were successfully oriented by taking advantage of surface interactions between the polymer and hydrophilic substrates, as confirmed by X-ray diffraction analysis (XRD), environmental scanning electron microscopy (ESEM) and optical microscopy between crossed polars (POM). Column orientation was found to be crucial for effective transport: the oriented membranes exhibited proton transport comparable to that of Nafion® N117 and no water uptake. An increase in sodium ion concentration in the feed phase suggested a proton/cation antiport. On the contrary, no proton transport was detected on unoriented membranes based on the same liquid-crystalline side-chain polyether or on unmodified PECH.     

Heat Transfer and Microbial Kinetics Modeling to Determine the Location of Microorganisms within Cucumber Fruit

ABSTRACT: Microbiological and modeling (combined heat transfer and microbial kinetic equations [HTMK] model) approaches were used to determine the location of microorganisms within cucumber. The total number of aerobes found within/on cucumbers varied from 10⁵ to 10⁷ colony-forming units (CFU) /g. The highest and the least amount of microbiota were respectively found at the blossom end and middle part of the cucumbers and were within the 1st 6 mm of the cucumber surface. A comparison of the calculated thermal death time from the HTMK model with the values obtained from experimental data showed that total aerobic microorganisms were located within 0.65 mm of the fruit surface.     

OWLS: a ten-year history in optical wireless links for intra-satellite communications

The application of Optical Wireless Links to intra- Spacecraft communications (OWLS) is presented here. This work summarizes ten years of developments, ranging from basic optoelectronic parts and front-end electronics, to different inorbit demonstrations. Several wireless applications were carried out in representative environments at ground level, and on in-flight experiments. A completely wireless satellite will be launched at the beginning of 2010. The benefits of replacing standard data wires and connectors with wireless systems are: mass reduction, flexibility, and simplification of the Assembly, Integration and Tests phases (AIT). However, the Aerospace and Defense fields need high reliability solutions. The use of COTS (Commercial-Off-The- Shelf) parts in these fields require extensive analyses in order to attain full product assurance. The current commercial optical wireless technology needs a deep transformation in order to be fully applicable in the aforementioned fields. Finally, major breakthroughs for the implementation of optical wireless links in Space will not be possible until dedicated circuits such as mixed analog/digital ASICs are developed. Once these products become available, it will also be possible to extend optical wireless links to other applications, such as Unmanned Air and Underwater Vehicles (UAV and UUV). The steps taken by INTA to introduce Optical Wireless Links in the Space environment are presented in this paper.     

Effect of IGF-II genotype and pig rearing system on the final characteristics of dry-cured Iberian hams

The effect of the IGF-II genotype (AG vs. GG) on the morphological and compositional parameters, the fatty acid composition of intramuscular fat, the odour concentration (analysed by dynamic olfactometry) and the volatile compound profile (analysed by proton transfer reaction time-of-flight mass spectrometry) of dry-cured Iberian ham was studied for the first time, and compared to the effect of pig rearing system (high-oleic concentrated feed vs. acorn and grass). The IGF-II genotype had no effect on most variables. However, it influenced the concentration of some odorants (methanethiol and octanal), although it did not affect odour concentration. Conversely, the rearing system had a significant effect on a large number of ham variables. Results indicate a negligible effect of the IGF-II genotype on the final ham quality and confirm that the rearing system has a marked effect.     

Comparative Effects of Graphene and Molybdenum Disulfide on Human Macrophage Toxicity

Graphene and other 2D materials, such as molybdenum disulfide, have been increasingly used in electronics, composites, and biomedicine. In particular, MoS₂ and graphene hybrids have attracted a great interest for applications in the biomedical research, therefore stimulating a pertinent investigation on their safety in immune cells like macrophages, which commonly engulf these materials. In this study, M1 and M2 macrophage viability and activation are mainly found to be unaffected by few‐layer graphene (FLG) and MoS₂ at doses up to 50 µg mL^?1. The uptake of both materials is confirmed by transmission electron microscopy, inductively coupled plasma mass spectrometry, and inductively coupled plasma atomic emission spectroscopy. Notably, both 2D materials increase the secretion of inflammatory cytokines in M1 macrophages. At the highest dose, FLG decreases CD206 expression while MoS₂ decreases CD80 expression. CathB and CathL gene expressions are dose‐dependently increased by both materials. Despite a minimal impact on the autophagic pathway, FLG is found to increase the expression of Atg5 and autophagic flux, as observed by Western blotting of LC3‐II, in M1 macrophages. Overall, FLG and MoS₂ are of little toxicity in human macrophages even though they are found to trigger cell stress and inflammatory responses.     

A two-stage mobile robot localization method by overlapping segment-based maps

This paper presents a new method for accurately estimating the pose (position and orientation) of a mobile robot by registering a segment-based local map observed from the current robot pose and a global map. The method works in a two-stage procedure. First, the orientation is determined by aligning the local and global map through a voting process based on a generalized Hough transform. Second, it uses a coarse-to-fine approach for selecting candidate positions and a weighted voting scheme to determine the degree of overlap of the two maps at each of these poses. Unlike other methods previously proposed, this approach allows us to uncouple the problem of estimating the robot orientation and the robot position which may be useful for some applications. In addition it can manage environments described by many (possibly short) segments. This paper presents some experimental results based on our mobile robot RAM-2 that show the accuracy and the robustness of the proposed method even for poor quality maps and large dead-reckoning errors.     

3D registration of laser range scenes by coincidence of coarse binary cubes

Scene registration of a pair of three-dimensional (3D) range images is a 6D optimization problem usually required in mobile robotics. This problem is different from object registration, since all scan directions and depths may contain relevant data, and because farther regions are sampled with lower densities. The paper proposes an efficient scene matching method based on the concept of coarse binary cubes. An integer objective function is defined as the number of coincident cubes between both scans. This is a metric of the degree of overlap that does not employ point distances. Its value is obtained without actually using any 3D grid data structure, with a computational complexity of order O(n), where n represents the number of laser points. This objective function is optimized with a globalized version of the downhill Simplex algorithm to avoid local minima. Experimental results are presented from indoor and outdoor environments with different degrees of structuring. The effect of cube size and the number of vertices on registration performance has been analyzed. Besides, experiments show that the proposed method achieves similar accuracy as iterative closest points (ICP) and normal distribution transform (NDT), while it improves both computation time and robustness against initial misalignments.     

The Chemokine Receptor CCR3 Is Potentially Involved in the Homing of Prostate Cancer Cells to Bone: Implication of Bone-Marrow Adipocytes

Bone metastasis remains the most frequent and the deadliest complication of prostate cancer (PCa). Mechanisms leading to the homing of tumor cells to bone remain poorly characterized. Role of chemokines in providing navigational cues to migrating cancer cells bearing specific receptors is well established. Bone is an adipocyte-rich organ since 50 to 70% of the adult bone marrow (BM) volume comprise bone marrow adipocytes (BM-Ads), which are likely to produce chemokines within the bone microenvironment. Using in vitro migration assays, we demonstrated that soluble factors released by human primary BM-Ads are able to support the directed migration of PCa cells in a CCR3-dependent manner. In addition, we showed that CCL7, a chemokine previously involved in the CCR3-dependent migration of PCa cells outside of the prostate gland, is released by human BM-Ads. These effects are amplified by obesity and ageing, two clinical conditions known to promote aggressive and metastatic PCa. In human tumors, we found an enrichment of CCR3 in bone metastasis vs. primary tumors at mRNA levels using Oncomine microarray database. In addition, immunohistochemistry experiments demonstrated overexpression of CCR3 in bone versus visceral metastases. These results underline the potential importance of BM-Ads in the bone metastatic process and imply a CCR3/CCL7 axis whose pharmacological interest needs to be evaluated.