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41.
42.
Dmitrii N. Ilin Nicolas Saintier Jean-Marc Olive Remi Abgrall Isabelle Aubert 《International Journal of Hydrogen Energy》2014
The effect of microstructure-induced strain-stress heterogeneity on the evolution of hydrogen distribution was studied on 316 L stainless steel. A crystal plasticity-transient hydrogen diffusion finite element analysis was developed. To reach the stress-strain heterogeneity, synthetic microstructures were computed under uniaxial loading. The results show that hydrostatic stress gradients is the main cause of the hydrogen redistribution and the strain rate has a crucial effect on the redistribution degree. Statistical analysis of H-segregation reveals clustering of H-enriched zones at the mesoscopic scale. 相似文献
43.
A procedure has been developed to separate and quantitate phospholipids, including phosphatidylinositol and phosphatidylserine,
from animal tissues by means of the Iatroscan TLC/FID technique. The method is based on the use of 0.01 M oxalic acid impregnated
Chromarods-SII and stepwise resolution of the phospholipids in the presence of 1,2-dipalmitoyl-sn-glycero-3-phospho (N,N-dimethylethanolamine) as internal standard. To remove the neutral lipids, the rods are initially developed in a nonpolar
solvent mixture followed by partial scanning. Next, the rods are impregnated with oxalic acid, developed twice in CHCl3/CH3OH/CH3COOH/HCOOH/H2O (80∶35∶2∶1∶3, v/v/v/v/v) and partially scanned for measuring lysophosphatidylcholine, sphingomyelin and phosphatidylcholine.
The subsequent step involves double development in CHCl3/CH3OH/30% NH4OH (60∶35∶0.9, v/v/v) to resolve cardiolipin, internal standard, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine
and phosphatidic acid. For each phospholipid a linear calibration curve with a highly significant correlation coefficient
was obtained. However, the calibration lines extrapolated to negative intercepts on the ordinate, indicating declining sensitivity
at low phospholipid loads. 相似文献
44.
Naoki Iwamoto Kaori Furukawa Yushiro Endo Toshimasa Shimizu Remi Sumiyoshi Masataka Umeda Tomohiro Koga Shin-ya Kawashiri Takashi Igawa Kunihiro Ichinose Mami Tamai Tomoki Origuchi Atsushi Kawakami 《International journal of molecular sciences》2021,22(21)
We aimed to investigate the effect of methotrexate (MTX) on microRNA modulation in rheumatoid arthritis fibroblast-like synovial cells (RA-FLS). RA-FLS were treated with MTX for 48 h. We then performed miRNA array analysis to investigate differentially expressed miRNAs. Transfection with miR-877-3p precursor and inhibitor were used to investigate the functional role of miR-877-3p in RA-FLS. Gene ontology analysis was used to investigate the cellular processes involving miR-877-3p. The production of cytokines/chemokines was screened by multiplex cytokine/chemokine bead assay and confirmed by ELISA and quantitative real-time PCR. The migratory and proliferative activities of RA-FLS were analyzed by wound healing assay and MKI-67 expression. MTX treatment altered the expression of 13 miRNAs (seven were upregulated and six were downregulated). Among them, quantitative real-time PCR confirmed that miR-877-3p was upregulated in response to MTX (1.79 ± 0.46-fold, p < 0.05). The possible target genes of miR-877-3p in RA-FLS revealed by the microarray analysis were correlated with biological processes. The overexpression of miR-877-3p decreased the production of GM-CSF and CCL3, and the overexpression of miR-877-3p inhibited migratory and proliferative activity. MTX altered the miR-877-3p expression on RA-FLS, and this alteration of miR-877-3p attenuated the abundant production of cytokines/chemokines and proliferative property of RA-FLS. 相似文献
45.
Chui Michael H.; Papanikolaou Yanni; Fontaine-Bisson Bénédicte; Turcotte Josée; Wolever Thomas M. S.; El-Sohemy Ahmed; Chiasson Jean-Louis; Rabasa-Lhoret Remi; Maheux Pierre; Ryan Edmond; Greenwood Carol E. 《Canadian Metallurgical Quarterly》2007,121(3):619
Inflammatory markers predict memory dysfunction in elderly patients, but their contribution to memory deficits in adults with Type 2 diabetes mellitus (T2DM) is less well understood. The present study determined whether specific single-nucleotide polymorphisms in the promoter region of tumor necrosis factor-alpha (TNF-α) predict verbal memory in older patients with T2DM. Immediate and delayed verbal memory were assessed using word list and paragraph recall tests in a cohort of subjects with T2DM during 2 sessions, separated by 48 weeks. The presence of the TNF-α-238A allele, which has been shown to decrease gene expression, consistently predicted better baseline performance and protected against memory decline over a period of 48 weeks. Therefore, inflammatory mediators may be important modulators of memory function in individuals with T2DM. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
46.
Guillaume Brousse Gilles Arnaud‐Fassetta Frdric Libault Mlanie Bertrand Gabriel Melun Remi Loire Jean‐Ren Malavoi Guillaume Fantino Laurent Borgniet 《河流研究与利用》2020,36(6):880-893
The Saint‐Sauveur dam was built in 1992 in the middle section of the Buëch River. Downstream of the dam, a channel incision by several meters was observed. A gravel replenishment operation was planned in order to restore the active channel. An equivalent of two times the mean annual bedload‐transport capacity (43,500 m3) was replenished downstream of the dam in September 2016. The aim of this paper is to quantify morphological change associated with sediment remobilization in order to evaluate the efficiency of the restoration works. The monitoring was based on a combination of (a) change detection using sequential high‐resolution digital elevation models (from airborne LiDAR data), (b) bedload tracing using active ultrahigh‐frequency radio‐frequency identification technology, and (c) complementary field surveys of channel grain‐size distribution and morphology for bedload‐transport computation. Field monitoring allows us to capture a net aggradation along a 2‐km reach after the first post‐replenishment flood. A sediment balance analysis was performed to back‐calculate bedload supply coming from the sluicing operation during the flood. Although the sediment replenishment operation clearly had a positive impact on the morphological conditions of the starved river reach, the effective bedload supply from artificial berms (22,650 m3) was insufficient to initiate substantial channel shifting along the restored reach and a subsequent amplification of the sediment recharge. The combination of high‐resolution topographic resurveys and sediment tracing was successful to evaluate the downstream propagation of sediment replenishment effects. 相似文献
47.
Intact washed human platelets aggregated in response to paf-acether (paf) and did not metabolize [3H]paf at concentrations up to 10 nM. However, when platelets were lysed by exposure to pH 9.5, resulting in 37.5±2.5% (mean
±SD, n=3) lactic dehydrogenase (LDH) release, 20.5±5.7% of the radioactivity was detected as labeled lyso paf and 5.7±3.1%
as labeled alkylacylglycerophosphocholine. When platelets were aggregated with 0.5 IU/mL thrombin or high concentrations of
paf (100 nM), they released a part of their acetylhydrolase without releasing LDH. In supernatants obtained from aggregated
platelets, 21±2% or 10±2% (n=3), respectively, of the total platelet acetylhydrolase activity was detectedvs. none in supernatants of resting cells. The release of acetylhydrolase was concentration-and time-dependent and paralleled
the release of PF 4, a marker for α-granules. The acetylhydrolase affinity for paf (Km) measured in sonicates of resting and thrombin-activated platelets was 8.3±1.5 μMvs. 10.6±1.5 μM, n=5, n.s. in a “Mann Whitney” test. The latter Km was slightly but significantly different (P<0.05, n=5) from that of the thrombin-released acetylhydrolase (7.9 ±1.5 μM) and that of the latter was itself different from
plasma acetylhydrolase (5.3±0.5,P<0.05, n=5). Addition of plasma (acid-treated to inactivate acetylhydrolase) decreased the Km value of supernatant acetylhydrolase to 6.1±1.4 μM. All preparations of acetylhydrolase exhibited similar pH requirements
and sensitvity to various inhibitors. Thus paf and thrombin cause release of acetylhydrolase from platelets in parallel with
release of the α-granule marker PF4. This phenomenon might represent a protective mechanism against paf-mediated effects in
thrombotic and cardiovascular diseases.
This study has been presented in part as a preliminary report at the 72nd Annual Meeting of the Federation of American Societies
for Experimental Biology, Las Vegas, NV, May 1988 (1). 相似文献
48.
Two-fluid plasma equations are derived by taking moments of Boltzmann equations. Ignoring collisions and viscous terms and assuming local thermodynamic equilibrium we get five moment equations for each species (electrons and ions), known as two-fluid plasma equations. These equations allow different temperatures and velocities for electrons and ions, unlike ideal magnetohydrodynamics equations. In this article, we present robust second order MUSCL schemes for two-fluid plasma equations based on Strang splitting of the flux and source terms. The source is treated both explicitly and implicitly. These schemes are shown to preserve positivity of the pressure and density. In the case of explicit treatment of source term, we derive explicit condition on the time step for it to be positivity preserving. The implicit treatment of the source term is shown to preserve positivity, unconditionally. Numerical experiments are presented to demonstrate the robustness and efficiency of these schemes. 相似文献
49.
Giuseppe Rossini Dario Rossi Christophe Betoule Remi Clavier Gilles Thouenon 《Computer Networks》2013,57(14):2802-2816
In this work, we propose Aplasia, an holistic architecture with a radical design. Aiming at simplifying the inner network devices (and so their cost), we tradeoff node architecture- and algorithmic-complexity for an increased (but tunable) communication cost. The main ingredients of our recipe are (i) the use of complete paths directly in the frames header, that allows core devices to perform data-plane switching functions without lookup and (ii) the use of a greedy probabilistic routing algorithm to quickly discover multiple, near optimal, paths in the control plane. We extensively simulate, analyze and implement our proposal to testify its soundness. 相似文献
50.
Sanderson H Dyer SD Price BB Nielsen AM van Compernolle R Selby M Stanton K Evans A Ciarlo M Sedlak R 《The Science of the total environment》2006,368(2-3):695-712
Alkyl sulfates (AS), alkyl ethoxysulfates (AES) and linear alkyl benzene sulfonates (LAS) are all High Production Volume (HPV) and 'down-the-drain' chemicals used globally in detergent and personal care products, resulting in low levels ultimately released to the environment via wastewater effluent. Due to their surfactant properties, they preferentially sorb to sediments. Hence, assessment of their levels and potential perturbations on benthos are of interest. The relative levels of AS/AES decreased with distance from the wastewater treatment plant outfall. However, this was not evident for LAS. Short chained AES and especially AS dominated the homologue distribution for AES. There were no evident patterns in LAS homologue distribution. The overall mean margin of exposure (MoE) for AS/AES and LAS is approximately 40 (range: 3 to 100) suggesting no noteworthy perturbation on biota. The findings in this study are in concordance with previous preliminary hazard screening. Comparative sediment contamination analyses principally based on Chapman and Anderson [Chapman PM, Anderson, J. A decision-making framework for sediment contamination. Integr Environ Assess Mana. 2005; 1: 163-173.] and the U.S. Environmental Protection Agency RAPID assessment methods [USEPA. Rapid bioassessment protocols for use in wadeable streams and rivers: Periphyton, benthic, macroinvertebrates, and fish. 1999. Second Edition. U.S. Environmental Protection Agency Office of Water, Washington, D.C. EPA 841-B-99-002.] did not reveal significant correlations between the surfactant concentrations and ecological status of the sampling locations. Several Lines of Evidence (LoE) of the Weight-of-Evidence (WoE) lead to the conclusion of low aquatic risk associated to the monitored compounds. 相似文献