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101.
A sulfated-β-cyclodextrin (s-β-CD) modified reduced flow micellar electrokinetic chromatography (RF-MEKC) method was developed and validated for the determination of catechins in green tea. The optimal electrolyte consisted of 0.2% triethylamine, 50 mmol/L SDS and 0.8% s-β-CD (pH = 2.9), allowing baseline separation of five catechins in 4 min. The samples and standards were injected at 0.6 psi for 5 s under constant voltage of −30 kV. Sample preparation simply involved extraction of 2 g of tea with 200 mL water at 95 °C under constant stirring for 5 min. The method demonstrated excellent performance, with limits of detection (LOD) and quantification (LOQ) of 0.02–0.1 and 0.1–0.5 μg/mL, respectively, and recovery percentages of 94–101%. The method was applied to six samples of Brazilian green tea infusions. Epigallocatechin gallate (23.4–112.4 μg/mL) was the major component, followed by epigallocatechin (18.4–78.9 μg/mL), epicatechin gallate (5.6–29.6 μg/mL), epicatechin (4.6–14.5 μg/mL) and catechin (3.2–8.2 μg/mL). 相似文献
102.
de Souza Junior Jonas Pereira Frazão Joaquim José de Morais Thais Chagas Barros Espoti Clara Degli dos Santos Sarah Marcilene Machado de Mello Prado Renato 《SILICON》2021,13(4):1269-1275
Silicon - Silicon (Si) combined with foliar spraying of salicylic acid (SA) can affect the absorption of this beneficial element. A study with Si non-accumulators plants (soybean and bean) observed... 相似文献
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Aline Moreira Portela de Melo María López-Pedrouso Roberto Germano Costa Daniel Franco Arlindo de Alencar Araripe Noronha Moura Taciane Alves da Silva Frederico Bruno Mendes Batista Moreno Valdi de Lima Júnior Ana Cristina de Oliveira Monteiro-Moreira Ariosvaldo Nunes de Medeiros Renato de Azevedo Moreira Jose M. Lorenzo 《International Journal of Food Science & Technology》2020,55(3):995-1001
A diet based on sunflower cake for lambs was assayed in order to reuse biodiesel industrial by-products with the aim of reducing livestock costs and evaluating their influence on meat quality. To achieve these goals, sixteen male lambs were fed diets containing different levels of sunflower cake (control, 5%, 10% and 15%). Afterwards, their semimembranosus muscles were analysed by two-dimensional electrophoresis coupled to mass spectrometry and their functional protein association was examined using STRING. Structural and metabolic proteins in the lambs’ proteomes changed significantly according to their diet. Fifteen proteins showed significant changes caused by the inclusion of sunflower cake, and the most differentially abundant structural proteins were detected in 2-DE gels from the lambs. Differentially abundant metabolic proteins such as ENO3 (enolase 3), MDH1 (malate dehydrogenase) and ALDH1A1 (retinal dehydrogenase) have been proposed as biomarkers of quality parameters in other species. 相似文献
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A strategy for improving peroxidase stability via immobilization on surface modified multi‐walled carbon nanotubes 下载免费PDF全文
107.
Pâmela Maria Moreira Fonseca Paulo Luiz De Sá Júnior Walter Miyakawa Álvaro José Damião L.H. Moreira da Silva Melo Renato Amaro Zângaro 《臭氧:科学与工程》2019,41(3):242-249
Ozonated water has been demonstrated to induce significant results in terms of the elimination of microorganisms. The present study assessed the damage to Streptococcus mutans after exposure to ozonated water; the ozone generator was adjusted to provide an outlet concentration of 60 mg/L, the samples were submitted to different ozonation times 1, 2, 4, 6, and 10 mi. Scanning electron microscopy and atomic force images were obtained to identify damage to the bacteria, followed by reactive oxygen species (ROS) evaluation and microbial viability. The results showed a significant reduction in viability and the images evidenced the generation of gaps on the microbial wall and surface layer alterations. Ozone can induce significant damage to S. mutans, thus suggesting that the use of ozonated water to prevent carious lesion formation is extremely promising. 相似文献
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Luana Machado Barros Ana Lúcia Nunes Gutjahr Ruth Leila Ferreira‐ Keppler Renato Tavares Martins 《Microscopy research and technique》2019,82(3):178-189
In the dipteran genus Hermetia, only 6 of the 78 valid species have documented immature stages: H. albitarsis Fabricius, 1805, H. aurata Bellardi, 1859, H. concinna Williston, 1900, H. illucens (Linnaeus, 1758), H. panamensis Greene, 1940 and H. pulchra Weidemann, 1830. In particular, H. illucens stands out due to its reported applicability for forensic, medical and economic purposes. Here, we described the morphology of eggs and immature stages of this species, with a view to detecting differences between instars and in the pupal stage, which should eventually help properly identifying larval age. We utilized both optical and scanning electron microscopy tools. The eggs are elliptical and elongated, and color varies from cream white to yellowish. The larvae are apodal, hemichephalic and holopneustic, flattened dorso‐ventrally and may be recognized by the head elongated, dorsal and ventral chaetotaxy of the cephalic capsule, thoracic and abdominal segments, and the morphology of the anterior and posterior spiracles. The pupae are adecticous and coarctate, tegument dark brown and pruinescence varying from brown to golden. The overall morphology across instars is similar, but marked variations were observed in the shape of the antennal articuli and the shape of the setae (first instar compared to the others). Our results supplement the biological information on Hermetia illucens and should aid the proper identification and aging of juveniles in the field, as a way to minimize errors in the calculation of the post‐mortem interval. 相似文献
109.
Erivan de Souza Oliveira Mighay Lovera Valessa Rios Pires Francisco Rogênio da Silva Mendes Nadya Virginia Lima Peixoto Maia João Pedro Viana Rodrigues Maria do Socorro Rocha Bastos Huai N. Cheng Atanu Biswas Renato de Azevedo Moreira Ana Cristina de Oliveira Monteiro Moreira 《Journal of Food Processing and Preservation》2022,46(3):e16408
110.
Gaspar João Ferreira Renato Sebastião Pedro Souto Nuno 《Wireless Personal Communications》2020,115(4):2729-2754
Wireless Personal Communications - The increased use of unmanned aerial vehicles (UAVs), better known as drones, by civilians has grown exponentially and their autonomous flight control systems... 相似文献