Rapeseed Proteins for Paperboard Coating

Protein samples of rapeseed were prepared with different compositions as protein isolate, protein concentrate and acetylated protein concentrate. They showed functional properties which are consolidated in the coating. The manufactured protein samples were tested in a pilot‐scale paperboard coating plant. The results indicated that the use of rapeseed proteins in the coating preparation is technically feasible and that the color and roughness of the coated paperboard show no negative effect on the quality. Industry printing experiments with this coated paperboard achieved the standard quality.     

Experimental setup for lensless imaging via soft x-ray resonant scattering

We have developed a setup for measuring holographically formed interference patterns using an integrated sample-mask design. The direct space image of the sample is obtained via a two-dimensional Fourier transform of the X-ray diffraction pattern. We present the details of our setup, commenting on the influence of geometrical parameters on the imaging capabilities. As an example, we present and discuss the results of test experiments on a patterned Co film.     

A new identification method of viscoelastic behavior: Application to the generalized Maxwell model

This paper focuses on the generalized Maxwell model (GMM) identification. The formulation of the transfer function of the GMM is defined, as well as its asymptotes. To compare identification methods of the parameters of the GMM, a test transfer function and two quality indicators are defined. Then, three graphical methods are described, the enclosing curve method, the CRONE method and an original one. But the results of graphical methods are not good enough. Thus, two optimization recursive processes are described to improve the results of graphical methods. The first one is based on an unconstrained non-linear optimization algorithm and the second one is original and allows constraining identified parameters. This new process uses the asymptotes of the modulus and the phase of the transfer function of the GMM. The result of the graphical method optimized with the new process is very accurate and fast.     

Update strategies for kriging models used in variable fidelity optimization

Many optimization methods for simulation-based design rely on the sequential use of metamodels to reduce the associated computational burden. In particular, kriging models are frequently used in variable fidelity optimization. Nevertheless, such methods may become computationally inefficient when solving problems with large numbers of design variables and/or sampled data points due to the expensive process of optimizing the kriging model parameters in each iteration. One solution to this problem would be to replace the kriging models with traditional Taylor series response surface models. Kriging models, however, were shown to provide good approximations of computer simulations that incorporate larger amounts of data, resulting in better global accuracy. In this paper, a metamodel update management scheme (MUMS) is proposed to reduce the cost of using kriging models sequentially by updating the kriging model parameters only when they produce a poor approximation. The scheme uses the trust region ratio (TR-MUMS), which is a ratio that compares the approximation to the true model. Two demonstration problems are used to evaluate the proposed method: an internal combustion engine sizing problem and a control-augmented structural design problem. The results indicate that the TR-MUMS approach is very effective; on the demonstration problems, it reduced the number of likelihood evaluations by three orders of magnitude compared to using a global optimizer to find the kriging parameters in every iteration. It was also found that in trust region-based method, the kriging model parameters need not be updated using a global optimizer—local methods perform just as well in terms of providing a good approximation without affecting the overall convergence rate, which, in turn, results in a faster execution time.     

Cell culture imaging using microimpedance tomography

We present a novel, inexpensive, and fast microimpedance tomography system for two-dimensional imaging of cell and tissue cultures. The system is based on four-electrode measurements using 16 planar microelectrodes (5 microm x 4 mm) integrated into a culture chamber. An Agilent 4294A impedance analyzer combined with a front-end amplifier is used for the impedance measurements. Two-dimensional images are obtained using a reconstruction algorithm. This system is capable of accurately resolving the shape and position of a human hair, yielding vertical cross sections of the object. Human epithelial stem cells (YF 29) are also grown directly on the device surface. Tissue growth can be followed over several days. A rapid resistivity decrease caused by permeabilized cell membranes is also monitored, suggesting that this technique can be used in electroporation studies.     

Characteristic quantities of microvascular structures in CLSM volume datasets

A method for fully automated morphological and topological quantification of microvascular structures in confocal laser scanning microscopy (CLSM) volume datasets is presented. Several characteristic morphological and topological quantities are calculated in a series of image-processing steps and can be used to compare single components as well as whole networks of microvascular structures to each other. The effect of the individual image-processing steps is illustrated and characteristic quantities of measured volume datasets are presented and discussed.     

Small group multicast: a new solution for multicasting on theInternet

The Internet's global ubiquity has fostered numerous applications that use many different communications models. Applications like FTP Web browsing, and e-mail employ a unicast model where two parties exchange data over logical point-to-point connections. In other applications, such as multiparty audio/video conferencing and collaborative gaming, a source sends data to multiple parties. One way to support multiparty communications is with unicast connections between the source and all of the receivers. If a group has N parties, then a source must set up N-1 unicast connections and transmit the data N times over the network. When N is large, scalability becomes an issue for the source and the network. IP multicast solves this problem by sending a single copy of the data over a distribution tree that is rooted at the source and that branches out to the various destinations. Because the source transmits a single copy of the data, only one copy of the data appears on the branches in the distribution tree     

Electrical power from sea and river water by reverse electrodialysis: a first step from the laboratory to a real power plant

Electricity can be produced directly with reverse electrodialysis (RED) from the reversible mixing of two solutions of different salinity, for example, sea and river water. The literature published so far on RED was based on experiments with relatively small stacks with cell dimensions less than 10 × 10 cm(2). For the implementation of the RED technique, it is necessary to know the challenges associated with a larger system. In the present study we show the performance of a scaled-up RED stack, equipped with 50 cells, each measuring 25 × 75 cm(2). A single cell consists of an AEM (anion exchange membrane) and a CEM (cation exchange membrane) and therefore, the total active membrane area in the stack is 18.75 m(2). This is the largest dimension of a reverse electrodialysis stack published so far. By comparing the performance of this stack with a small stack (10 × 10 cm(2), 50 cells) it was found that the key performance parameter to maximal power density is the hydrodynamic design of the stack. The power densities of the different stacks depend on the residence time of the fluids in the stack. For the large stack this was negatively affected by the increased hydrodynamic losses due to the longer flow path. It was also found that the large stack generated more power when the sea and river water were flowing in co-current operation. Co-current flow has other advantages, the local pressure differences between sea and river water compartments are low, hence preventing leakage around the internal manifolds and through pinholes in the membranes. Low pressure differences also enable the use of very thin membranes (with low electrical resistance) as well as very open spacers (with low hydrodynamic losses) in the future. Moreover, we showed that the use of segmented electrodes increase the power output by 11%.     

Phase-sensitive spatially-modulated surface plasmon resonance polarimetry for detection of biomolecular interactions

We here describe a scheme of spatially modulated surface plasmon resonance (SPR) polarimetry that enables to combine ultra-high phase sensitivity with good signal-to-noise background. The proposed approach uses spatial modulation of s-polarized component by birefringent elements and the extraction of phase-polarization information by Fourier-transform methods. This scheme was tested for monitoring the interactions between an antibody and its biological partner. Our experimental data, collected by amplitude-sensitive and phase-sensitive polarimetry demonstrate that the latter scheme provides at least one order of magnitude improvement in terms of detection limit.