Antonio Gentile  Chiara Ferrara  Sergio Tosoni  Marcella Balordi  Stefano Marchionna  Federico Cernuschi  Min‐Ho Kim  Hyun‐Wook Lee  Riccardo Ruffo 《Small Methods》2020,4(9)

  相似文献   

    

Riccardo Balzarotti  Alessandra Bisaccia  Maria Celeste Tripi  Matteo Ambrosetti  Gianpiero Groppi  Enrico Tronconi 《Journal of Advanced Manufacturing and Processing》2020,2(4):e10068

Additive manufacturing by 3D printing comprises a set of methods for production of 3D objects starting from a CAD file. Advantages of additive manufacturing combine high manufacturing resolution, a reduction of waste material, and the possibility of computer-aided design (CAD). When applied to the manufacturing of structured catalyst substrates, the latter enables the optimization of transport properties of the catalyst support. Despite several methods have been introduced for a variety of materials, copper, well known for its high thermal conductivity, is still difficult to be handled. In this work, a novel approach for the additive manufacturing of copper periodic open cellular structures (POCS) is proposed and investigated. It consists in the use of the replica manufacturing procedure starting from resin supports produced by 3D printing stereolithography. Micrometric high purity copper powder was effectively dispersed using a liquid medium based on organic components; the resulting slurry was used for the washcoat deposition on the resin supports. Structures with diamond unit cell shape (cell size of 2.5 mm and void fractions in the 0.8-0.9 range) were washcoated by dip-spin coating. Homogeneous washcoat layers were obtained without occurrence of cell clogging phenomena. Optimized thermal treatment procedure was assessed for sintering the copper POCS. The resulting matrices preserved the morphology of the original structure, reaching a resolution in the range of 70 to 120 μm. These materials can eventually be used as catalyst supports for heat-transfer limited applications (eg, steam reforming of methane), where copper-based substrates were demonstrated to be an effective solution for process intensification.  相似文献   

    

Riccardo Ruixi Chen  Yuanmiao Sun  Samuel Jun Hoong Ong  Shibo Xi  Yonghua Du  Chuntai Liu  Ovadia Lev  Zhichuan J. Xu 《Advanced materials (Deerfield Beach, Fla.)》2020,32(10):1907976

Exploring highly efficient catalysts for the oxygen evolution reaction (OER) is essential for water electrolysis. Cost-effective transition-metal oxides with reasonable activity are raising attention. Recently, OER reactants' and products' differing spin configurations have been thought to cause slow reaction kinetics. Catalysts with magnetically polarized channels could selectively remove electrons with opposite magnetic moment and conserve overall spin during OER, enhancing triplet state oxygen molecule evolution. Herein, antiferromagnetic inverse spinel oxide LiCoVO₄ is found to contain d⁷ Co²⁺ ions that can be stabilized under active octahedral sites, possessing high spin states S = 3/2 (t_2g⁵e_g²). With high spin configuration, each Co²⁺ ion has an ideal magnetic moment of 3 µ_B, allowing the edge-shared Co²⁺ octahedra in spinel to be magnetically polarized. Density functional theory simulation results show that the layered antiferromagnetic LiCoVO₄ studied contains magnetically polarized channels. The average magnetic moment (µ_ave) per transition-metal atom in the spin conduction channel is around 2.66 µ_B. Such channels are able to enhance the selective removal of spin-oriented electrons from the reactants during the OER, which facilitates the accumulation of appropriate magnetic moments for triplet oxygen molecule evolution. In addition, the LiCoVO₄ reported has been identified as an oxide catalyst with excellent OER activity.  相似文献   

    

Marco Monticelli  Andrea Torti  Matteo Cantoni  Daniela Petti  Edoardo Albisetti  Alessandra Manzin  Erica Guerriero  Roman Sordan  Giacomo Gervasoni  Marco Carminati  Giorgio Ferrari  Marco Sampietro  Riccardo Bertacco 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(7):921-929

Methods for the manipulation of single magnetic particles have become very interesting, in particular for in vitro biological studies. Most of these studies require an external microscope to provide the operator with feedback for controlling the particle motion, thus preventing the use of magnetic particles in high‐throughput experiments. In this paper, a simple and compact system with integrated electrical feedback is presented, implementing in the very same device both the manipulation and detection of the transit of single particles. The proposed platform is based on zig‐zag shaped magnetic nanostructures, where transverse magnetic domain walls are pinned at the corners and attract magnetic particles in suspension. By applying suitable external magnetic fields, the domain walls move to the nearest corner, thus causing the step by step displacement of the particles along the nanostructure. The very same structure is also employed for detecting the bead transit. Indeed, the presence of the magnetic particle in suspension over the domain wall affects the depinning field required for its displacement. This characteristic field can be monitored through anisotropic magnetoresistance measurements, thus implementing an integrated electrical feedback of the bead transit. In particular, the individual manipulation and detection of single 1‐μm sized beads is demonstrated.  相似文献   

    

Greta Radaelli  Jos Alejandro Heredia‐Guerrero  Muhammad T. Masood  Luca Ceseracciu  Alexander Davis  Riccardo Carzino  Mirko Prato  Ilker S. Bayer  Athanassia Athanassiou 《Advanced Materials Interfaces》2016,3(13)

A pH‐based method to tune the tribological and wetting properties of a coating obtained from water‐dispersed perfluorinated acrylic copolymer is demonstrated. The surface‐exposed fluorinated chains and chemical charges of the sprayed coatings can be controlled simply by tuning the pH of the initial water dispersion. The surface properties of the sprayed polymeric coatings remain unmodified when the pH of the water dispersion is reduced (addition of HCl). On the contrary, from dispersions with increased pH (addition of NaOH) a fluoroacrylic polyelectrolyte polymer is formed and clear variations in wetting and tribological properties of the polymeric coatings are observed. Interestingly, coatings' surface adhesion to vulcanizing rubber can be strongly reduced when the pH of the dispersions is in the range 4.5–6.0, which corresponds to a specific ratio between fluorinated chains and chemical charges. Consequently, such coatings can be proposed as low‐cost and easy‐to‐apply alternatives to aqueous release agents for the rubber tires demolding processes, thus having significant implications for the automotive tire industry.  相似文献   

    

Alessandro Marchioni  Roberto Tonelli  Alessandro Andreani  Gaia Francesca Cappiello  Matteo Fermi  Fabiana Trentacosti  Ivana Castaniere  Riccardo Fantini  Luca Tabbì  Dario Andrisani  Filippo Gozzi  Giulia Bruzzi  Linda Manicardi  Antonio Moretti  Serena Baroncini  Anna Valeria Samarelli  Massimo Pinelli  Giorgio De Santis  Alessandro Stefani  Daniele Marchioni  Francesco Mattioli  Enrico Clini 《International journal of molecular sciences》2022,23(5)

Laryngotracheal stenosis (LTS) is a complex and heterogeneous disease whose pathogenesis remains unclear. LTS is considered to be the result of aberrant wound-healing process that leads to fibrotic scarring, originating from different aetiology. Although iatrogenic aetiology is the main cause of subglottic or tracheal stenosis, also autoimmune and infectious diseases may be involved in causing LTS. Furthermore, fibrotic obstruction in the anatomic region under the glottis can also be diagnosed without apparent aetiology after a comprehensive workup; in this case, the pathological process is called idiopathic subglottic stenosis (iSGS). So far, the laryngotracheal scar resulting from airway injury due to different diseases was considered as inert tissue requiring surgical removal to restore airway patency. However, this assumption has recently been revised by regarding the tracheal scarring process as a fibroinflammatory event due to immunological alteration, similar to other fibrotic diseases. Recent acquisitions suggest that different factors, such as growth factors, cytokines, altered fibroblast function and genetic susceptibility, can all interact in a complex way leading to aberrant and fibrotic wound healing after an insult that acts as a trigger. However, also physiological derangement due to LTS could play a role in promoting dysregulated response to laryngo-tracheal mucosal injury, through biomechanical stress and mechanotransduction activation. The aim of this narrative review is to present the state-of-the-art knowledge regarding molecular mechanisms, as well as mechanical and physio-pathological features behind LTS.  相似文献   

    

Valentina Doldi  Mara Lecchi  Silva Ljevar  Maurizio Colecchia  Elisa Campi  Giovanni Centonze  Cristina Marenghi  Tiziana Rancati  Rosalba Miceli  Paolo Verderio  Riccardo Valdagni  Paolo Gandellini  Nadia Zaffaroni 《International journal of molecular sciences》2022,23(14)

Prostate cancer (PCa) ranges from indolent to aggressive tumors that may rapidly progress and metastasize. The switch to aggressive PCa is fostered by reactive stroma infiltrating tumor foci. Therefore, reactive stroma-based biomarkers may potentially improve the early detection of aggressive PCa, ameliorating disease classification. Gene expression profiles of PCa reactive fibroblasts highlighted the up-regulation of genes related to stroma deposition, including periostin and sparc. Here, the potential of periostin as a stromal biomarker has been investigated on PCa prostatectomies by immunohistochemistry. Moreover, circulating levels of periostin and sparc have been assessed in a low-risk PCa patient cohort enrolled in active surveillance (AS) by ELISA. We found that periostin is mainly expressed in the peritumoral stroma of prostatectomies, and its stromal expression correlates with PCa grade and aggressive disease features, such as the cribriform growth. Moreover, stromal periostin staining is associated with a shorter biochemical recurrence-free survival of PCa patients. Interestingly, the integration of periostin and sparc circulating levels into a model based on standard clinico-pathological variables improves its performance in predicting disease reclassification of AS patients. In this study, we provide the first evidence that circulating molecular biomarkers of PCa stroma may refine risk assessment and predict the reclassification of AS patients.  相似文献   

    

Davide Loizzo  Savio Domenico Pandolfo  Devin Rogers  Clara Cerrato  Nicola Antonio di Meo  Riccardo Autorino  Vincenzo Mirone  Matteo Ferro  Camillo Porta  Alessandro Stella  Cinzia Bizzoca  Leonardo Vincenti  Marco Spilotros  Monica Rutigliano  Michele Battaglia  Pasquale Ditonno  Giuseppe Lucarelli 《International journal of molecular sciences》2022,23(7)

Autophagy is a complex process involved in several cell activities, including tissue growth, differentiation, metabolic modulation, and cancer development. In prostate cancer, autophagy has a pivotal role in the regulation of apoptosis and disease progression. Several molecular pathways are involved, including PI3K/AKT/mTOR. However, depending on the cellular context, autophagy may play either a detrimental or a protective role in prostate cancer. For this purpose, current evidence has investigated how autophagy interacts within these complex interactions. In this article, we discuss novel findings about autophagic machinery in order to better understand the therapeutic response and the chemotherapy resistance of prostate cancer. Autophagic-modulation drugs have been employed in clinical trials to regulate autophagy, aiming to improve the response to chemotherapy or to anti-cancer treatments. Furthermore, the genetic signature of autophagy has been found to have a potential means to stratify prostate cancer aggressiveness. Unfortunately, stronger evidence is needed to better understand this field, and the application of these findings in clinical practice still remains poorly feasible.  相似文献   

    

Maria Addolorata Bonifacio  Riccardo Laterza  Angela Vinella  Annalisa Schirinzi  Mariangela Defilippis  Francesca Di Serio  Angelo Ostuni  Antonio Fasanella  Maria Addolorata Mariggi 《International journal of molecular sciences》2022,23(17)

Serological assays are useful in investigating the development of humoral immunity against SARS-CoV-2 in the context of epidemiological studies focusing on the spread of protective immunity. The plaque reduction neutralization test (PRNT) is the gold standard method to assess the titer of protective antibodies in serum samples. However, to provide a result, the PRNT requires several days, skilled operators, and biosafety level 3 laboratories. Therefore, alternative methods are being assessed to establish a relationship between their outcomes and PRNT results. In this work, four different immunoassays (Roche Elecsys^® Anti SARS-CoV-2 S, Snibe MAGLUMI^® SARS-CoV-2 S-RBD IgG, Snibe MAGLUMI^® 2019-nCoV IgG, and EUROIMMUN^® SARS-CoV-2 NeutraLISA assays, respectively) have been performed on individuals healed after SARS-CoV-2 infection. The correlation between each assay and the reference method has been explored through linear regression modeling, as well as through the calculation of Pearson’s and Spearman’s coefficients. Furthermore, the ability of serological tests to discriminate samples with high titers of neutralizing antibodies (>160) has been assessed by ROC curve analyses, Cohen’s Kappa coefficient, and positive predictive agreement. The EUROIMMUN^® NeutraLISA assay displayed the best correlation with PRNT results (Pearson and Spearman coefficients equal to 0.660 and 0.784, respectively), as well as the ROC curve with the highest accuracy, sensitivity, and specificity (0.857, 0.889, and 0.829, respectively).  相似文献   

    

Antonio Tursi  Valerio Papa  Loris Riccardo Lopetuso  Carlo Romano Settanni  Antonio Gasbarrini  Alfredo Papa 《International journal of molecular sciences》2022,23(23)

Gut microbiota (GM) composition and its imbalance are crucial in the pathogenesis of several diseases, mainly those affecting the gastrointestinal tract. Colon diverticulosis and its clinical manifestations (diverticular disease, DD) are among the most common digestive disorders in developed countries. In recent literature, the role of GM imbalance in the onset of the different manifestations within the clinical spectrum of DD has been highlighted. This narrative review aims to summarize and critically analyze the current knowledge on GM dysbiosis in diverticulosis and DD by comparing the available data with those found in inflammatory bowel disease (IBD). The rationale for using probiotics to rebalance dysbiosis in DD is also discussed.  相似文献