A rapid quenching apparatus employing a laser beam was developed to obtain the glassy state of oxides which have high melting temperatures. Using this apparatus, the glassy state in Ln-Ti-O, Ln-Nb-O and Ln-Ta-O (Ln=lanthanides) systems was investigated. The range of compositions which produced glasses and the crystallization process were studied in detail, and the metastable phases, which appear during the crystallization process, were examined by differential thermal and X-ray diffraction analysis. 相似文献
The mechanism of gap junction enhancer (PQ1) induced cytotoxicity is thought to be attributed to the change in connexin 43 (Cx43) expression; therefore, the effects of Cx43 modulation in cell survival were investigated in mammary carcinoma cells (FMC2u) derived from a malignant neoplasm of a female FVB/N-Tg(MMTV-PyVT)634Mul/J (PyVT) transgenic mouse. PQ1 was determined to have an IC50 of 6.5 µM in FMC2u cells, while inducing an upregulation in Cx43 expression. The effects of Cx43 modulation in FMC2u cell survival was determined through transfection experiments with Cx43 cDNA, which induced an elevated level of protein expression similar to that seen with PQ1 exposure, or siRNA to silence Cx43 protein expression. Overexpression or silencing of Cx43 led to a reduction or an increase in cell viability, respectively. The mitogen-activated protein kinase (MAPK) family has been implicated in the regulation of cell survival and cell death; therefore, the gap junctional intercellular communication (GJIC)-independent function of PQ1 and Cx43 in the Raf/Mitogen-activated protein kinase/ERK kinase/extracellular-signal-regulated kinase (Raf-MEK-ERK) cascade of cellular survival and p38 MAPK-dependent pathway of apoptosis were explored. PQ1 treatment activated p44/42 MAPK, while the overexpression of Cx43 resulted in a reduced expression. This suggests that PQ1 affects the Raf-MEK-ERK cascade independent of Cx43 upregulation. Both overexpression of Cx43 and PQ1 treatment stimulated an increase in the phosphorylated form of p38-MAPK, reduced levels of the anti-apoptotic protein Bcl-2, and increased the cleavage of pro-caspase-3. Silencing of Cx43 protein expression led to a reduction in the phosphorylation of p38-MAPK and an increase in Bcl-2 expression. The mechanism behind PQ1-induced cytotoxicity in FMC2u mammary carcinoma cells is thought to be attributed to the change in Cx43 expression. Furthermore, PQ1-induced apoptosis through the upregulation of Cx43 may depend on p38 MAPK, highlighting that the effect of PQ1 on gap junctions as well as cellular survival via a MAPK-dependent pathway. 相似文献
We examined the in vitro metabolism of (+)-terpinen-4-ol by human liver microsomes and recombinant enzymes. The biotransformation of (+)-terpinen-4-ol was investigated by gas chromatography-mass spectrometry (GC-MS). (+)-Terpinen-4-ol was found to be oxidized to (+)-(1R,2S,4S)-1,2-epoxy-p-menthan-4-ol, (+)-(1S,2R,4S)-1,2-epoxy-p-menthan-4-ol, and (4S)-p-menth-1-en-4,8-diol by human liver microsomal P450 enzymes. The identities of (+)-terpinen-4-ol metabolites were determined through the relative abundance of mass fragments and retention times on GC-MS. Of 11 recombinant human P450 enzymes tested, CYP1A2, CYP2A6, and CYP3A4 were found to catalyze the oxidation of (+)-terpinen-4-ol. Based on several lines of evidence, CYP2A6 and CYP3A4 were determined to be major enzymes involved in the oxidation of (+)-terpinen-4-ol by human liver microsomes. First, of the 11 recombinant human P450 enzymes tested, CYP1A2, CYP2A6 and CYP3A4 catalyzed oxidation of (+)-terpinen-4-ol. Second, oxidation of (+)-terpinen-4-ol was inhibited by (+)-menthofuran and ketoconazole, inhibitors known to be specific for these enzymes. Finally, there was a good correlation between CYP2A6 and CYP3A4 activities and (+)-terpinen-4-ol oxidation activities in the 10 human liver microsomes. 相似文献
Combination of the reactions by means of membrane separation techniques are of interest. The CO2 methanation was combined with NH3 decomposition by in situ H2 separation through a Pd membrane. The CO2 methanation reaction in the permeate side was found to significantly enhance the H2 removal rate of Pd membrane compared to the use of sweep gas. The reaction rate of CO2 methanation was not influenced by H2 supply through the Pd membrane in contrast to NH3 decomposition in the retentate side. However, the CH4 selectivity could be improved by using a membrane separation technique. This would be caused by the active dissociated H species which might immediately react with adsorbed CO species on the catalysts to CH4 before those CO species desorbed. From the reactor configuration tests, the countercurrent mode showed higher H2 removal rate in the combined reaction at 673 K compared to the cocurrent mode but the reaction rate in CO2 methanation should be improved to maximize the perfomance of membrane reactor. 相似文献
Zucker fatty diabetes mellitus (ZFDM) rats harboring the missense mutation (fa) in a leptin receptor gene have been recently established as a novel animal model of obesity and type 2 diabetes (T2D). Here, we explored changes in cardiovascular dynamics including blood pressure and heart rate (HR) associated with the progression of obesity and T2D, as well as pathological changes in adipose tissue and kidney. There was no significant difference in systolic blood pressure (SBP) in ZFDM-Leprfa/fa (Homo) compared with ZFDM-Leprfa/+ (Hetero) rats, while HR and plasma adrenaline in Homo were significantly lower than Hetero. The mRNA expression of monocyte chemotactic protein-1 in perirenal white adipose tissue (WAT) from Homo was significantly higher than Hetero. Interscapular brown adipose tissue (BAT) in Homo was degenerated and whitened. The plasma blood urea nitrogen in Homo was significantly higher than Hetero. In summary, we demonstrated for the first time that HR and plasma adrenaline concentration but not SBP in Homo decrease with obesity and T2D. In addition, inflammation occurs in WAT from Homo, while whitening occurs in BAT. Further, renal function is impaired in Homo. In the future, ZFDM rats will be useful for investigating metabolic changes associated with the progression of obesity and T2D. 相似文献
This paper describes the numerical method of a distributed parameter analysis code SPIRAL for the calculation of fluid flow and temperature in arbitrary channel geometries, discusses the numerical method in the modeling and solution of the problem, and presents some results, including comparison with experiments.
The derivation and solution of the finite element equations is discussed. In order to overcome difficulties arising from the geometry, the Galerkin finite element method using isoparametric elements was employed, and a procedure of finite element generation using curvilinear coordinate system was developed.
The SPIRAL code permits calculation of the fine structure of the multi-dimensional steady-state single-phase fluid flow and temperature fields in LMFBR fuel pin subassemblies in the presence of wire spacers. Calculated results are presented for crossflow velocity distributions and crossflow pressure drop characteristics in a tube bundle geometry with and without wire spacers, natural convection and heat transfer in horizontal annuli, flow in a wire-wrapped 7-pin bundle geometry and fully developed turbulent flow in a parallel 4-rod array contained in a rectangular duct. 相似文献
The basidiomycete Lentinus edodes (Le.) ras gene (or its cDNA clone) [Hori et al., Gene 105 (1991) 91-96] was utilized to identify and clone the corresponding gene (Cc.ras)-containing genomic fragment from the basidiomycete, Coprinus cinereus. Cc.ras encodes 215 amino acids (aa) interrupted by six small introns. The deduced Cc.RAS protein exhibits significant homology (84.7% identical) to the Le.RAS protein (217 aa) in size and aa sequence. 相似文献