Apolipoprotein B is intracellularly associated with an ER-60 protease homologue in HepG2 cells

Two ALLN (N-acetyl-leucyl-leucyl-norleucinal)-sensitive endoplasmic reticulum (ER)-localized proteases (ER-60 and ER-72) were recently purified from rat liver. We used an antibody to rat ER-60 to investigate the possible role of this protease in apolipoprotein B (apoB) degradation. First, immunoprecipitation and immunoblotting experiments with the anti-rat ER-60 antibody suggested that HepG2 cells contain a homologue of ER-60 with an approximate molecular mass of 58-60 kDa. The ER-60 homologue was mostly associated with the luminal contents of HepG2 microsomes. Evidence from co-immunoprecipitation and cross-linking experiments appear to suggest that the ER-60 homologue in HepG2 cells is associated with apoB intracellularly. A small pool of apoB was recovered when HepG2 lysates were subjected to immunoprecipitation with anti-rat ER-60 antibody followed by a second immunoprecipitation with anti-apoB antibody. Furthermore, cross-linking of permeabilized cells with dithiobis(succinimidylpropionate) further demonstrated association of apoB with the ER-60 homologue in HepG2 cells. Three polypeptides with molecular masses of 78, 66, and 50 kDa were consistently found to be associated with apoB as well as the 58-kDa ER-60 homologue. The 78-kDa protein associated with both apoB and ER-60 appeared to represent immunoglobulin heavy chain-binding protein (BiP) based on immunoprecipitation with a monoclonal antibody. Cross-linking and immunoblotting experiments suggested the association of the 78-kDa BiP with both the 58-kDa ER-60 homologue as well as the 550-kDa apoB. In summary, the data suggests that HepG2 cells contain a 58-kDa protein which is homologous to the rat liver ER-60 in size, antigenecity, and intracellular localization. The ER-60 homologue in HepG2 cells appears to be closely associated with apoB, as well as other proteins possibly representing ER chaperones such as BiP. We hypothesize that the ER-60 homologue may be involved in the degradation of apoB in the ER lumen of HepG2 cells.     

A 2.488–11.2 Gb/s SerDes in 40 nm low-leakage CMOS with multi-protocol compatibility for FPGA applications

This paper presents the design and Silicon verification of a 2.488–11.2 Gbps multi-standard SerDes transceiver in a 40 nm low-leakage CMOS process. The paper explores the architectural and circuit techniques used to meet the stringent requirements of the high-speed SerDes and to mitigate the performance impact of the low-leakage process. A system modeling approach is described, which is used for optimizing the architectural trade-offs. The transceiver makes use of a low-jitter LC phase locked loop to enable high-reliability system design. The design has 420 fs RJ_rms and consumes 30.1 mW/Gbps at 11.2 Gbps.     

Rendezvous Point Relocation in Protocol Independent Multicast-Sparse Mode

Protocol Independent Multicast-Sparse Mode is the most widely used multicast routing architecture. It builds a shared distribution tree centered at a rendezvous point and then builds source-specific trees for those sources whose data rate warrants it. Current implementations of the protocol decide on the rendezvous point administratively, which leads to congestion and delays. An attractive solution would be dynamic relocation of the rendezvous point depending on the members of the multicast group. In this paper we present a rendezvous point calculation and relocation mechanism for Protocol Independent Multicast-Sparse Mode. Simulation of the proposed mechanisms is used to demonstrate the effectiveness of the proposals.     

High-resolution transmission electron microscopy study of electrically-driven reversible phase change in ge2sb2te5 nanowires

By combining high-resolution transmission electron microscopy (HRTEM) characterization and electrical measurements on a unique device platform, we study the reversible electrically-driven phase-change characteristics of self-assembled Ge(2)Sb(2)Te(5) nanowires. Detailed HRTEM analyses are used to correlate and understand the effect of full and intermediate structural transformations on the measured electrical properties of the nanowire devices. The study demonstrates that our unique approach has the potential to provide new information regarding the dynamic structural and electrical states of phase-change materials at the nanoscale, which will aid the design of future phase-change memory devices.     

Silicon Lens Optimization to Create Diffuse,Uniform Illumination from Incoherent THz Source Arrays

Journal of Infrared, Millimeter, and Terahertz Waves - Arrays of terahertz (THz) sources provide a pathway to overcoming the radiation power limitations of single sources. Several independent...     

Structural,morphological, and optical properties of strontium doped lead-free BCZT ceramics

We report the synthesis of Sr²⁺ doped Ba_0.9-xCa_0.1Sr_xTi_0.8Zr_0.2O₃ nano-ceramics by the conventional solid-state reaction method. Phase formation of single-phase orthorhombic ABO₃ type structure with space group P2mm was confirmed through X-ray diffraction (XRD). The crystallite size increased with increasing doping concentration from 25.46 nm to 52.96 nm as calculated by the Scherrer formula and from 47.1 nm to 88.5 nm by the Williamson-Hall method. The lattice parameter, dislocation density, and apparent density decreased with doping, except for when x = 0.05. The porosity was found to increase up to 16.8% with increasing doping. Field emission scanning electron microscopy (FESEM) shows that samples exhibit a flake-like structure. X-ray photoelectron spectroscopy (XPS) analysis confirms that Sr-ions occupy the Ca-site, for x = 0.05, and force the Ca ions to occupy the Ti-sites. For the higher concentration of Sr, i.e. x ≥ 0.15, no more forced substitution is observed and Sr-ions occupy the Ba-site only, which decreases oxygen vacancies. Diffused rings observed in selective area electron diffraction (SAED) patterns indicate the high crystalline order of the samples. The Fourier-transformed infrared spectroscopy (FTIR) measurements show a single broad peak between 544 and 594 cm^?1 for all the compositions, while two prominent peaks are observed for the composition x = 0.05 at 528 cm^?1 and 592 cm^?1. The Raman spectra show a shift in the most prominent peak, observed approximately 517 cm^?1.     

An investigation of molybdenum surface reinforcement on the hardness and wear properties of AISI 630

AISI 630 stainless steel (SS) surface has been reinforced with molybdenum (Mo) using gas tungsten arc (GTA) as a heat source. The optimum GTA heat source conditions have been finalised based on the proper fusion of base metal. The microhardness of the Mo-reinforced AISI 630 SS was found to be 502?HV which shows an improvement of 35% with respect to the base metal (371?HV). To improve the properties, the reinforced alloy was heat treated. The microhardness of the reinforced and aged AISI 630 was 725?HV, which was higher than the base metal by 95%. Dry sliding wear tests using a Pin-on-Disc machine were performed and it was found that the wear resistance of the reinforced surface was improved by 52% with respect to base metal. Characterisation techniques such as optical microscopy, scanning electron microscope, energy-dispersive X-ray spectrometry and XRD were used to establish morphology, structure and composition, and the presence of Mo was confirmed.     

Above-ground morphological predictors of rooting success in rooted cuttings of Jatropha curcas L.

Cuttings of 1–3 cm diameter and 45 cm length were collected during the first week of February from branches of previous year’s growth in a mature plantation of Jatropha curcas. The cuttings, without application of any growth regulator, were planted in nursery beds having loam: gravel (1:1 v/v) mixture rooting media. The nursery beds existed inside a polyethylene tunnel where intermittent misting was done. When sprouting percentage had stabilised, sprouted cuttings were removed from the media, and root and shoot characteristics of the cuttings were recorded. The number of roots and root length were found to be significantly correlated (P < 0.01) with one another as well as with sprout length, number of sprouts and number of leaves. The following equations were fitted for prediction of root characteristics of a cutting from of its above-ground characteristics: (i) No. of roots = −0.409 + 0.452 (no. of leaves) + 0.395 (sprout length), and (ii) Root length = 2.656 + 0.206 (no. of leaves) + 0.270 (sprout length); the sprout length and root length are in centimetres in both equations. Thicker cuttings possessed better root and above-ground characteristics.